洋葱伯克氏菌无细胞培养物的生防及促生活性研究

详细信息    查看全文 | 推荐本文 |

英文篇名：Studies of Bio-control and Plant Growth Promotion of the Cell-free Cultures of Burkholderia cepacia Complex 作者：郑梦晨 ; 尚金楠 ; 沈瑶 ; 杨晓霞 ; 包崇山 ; 方媛 英文作者：ZHENG Meng-chen;College of Chemistry & Life Sciences,Zhejiang Normal University; 关键词：洋葱伯克氏菌 ; 无细胞培养物 ; 拮抗 ; 促生 英文关键词：Burkholderia cepacia complex;;Cell-free cultures;;Antagonism;;Growth promotion 中文刊名：AHNY 英文刊名：Journal of Anhui Agricultural Sciences 机构：浙江师范大学化学与生命科学学院; 出版日期：2013-10-10 出版单位：安徽农业科学 年：2013 期：v.41;No.426 基金：浙江师范大学博士科研启动经费(ZC304013019) 语种：中文; 页：AHNY201329039 页数：3 CN：29 ISSN：34-1076/S 分类号：112-114

摘要

为了防止洋葱伯克氏菌在农业环境中对人类的危害,采用离心和过滤的方法获得洋葱伯克氏菌无细胞培养物,并研究其在平板上对植物病原真菌的拮抗作用和对水稻的促生作用。结果表明:洋葱伯克氏菌对植物病原真菌具有明显的拮抗作用,其中菌株R1、R15、R19、R20、R21、R25、R28的抑菌效果显著,在这7株菌中R15表现出对灰霉菌的强烈抑制作用和对稻瘟菌的较强抑制作用,并且表现出明显的促生作用。
        In order to avoid the risk of Burkholderia cepacia complex,the cell-free cultures of Burkholderia cepacia complex were collected by centrifuge and filter,the antagonisms to plant pathogenic fungi and the growth promotion to rice were studied on panel. The result showed that the Burkholderia cepacia complex used in this experiment performed obvious antagonistic activity to plant pathogenic fungi,in which the inhibitory effect of strain R1、R15、R19、R20、R21、R25、R28were extremely marked,and among these,R15shows intensive inhibition effect to Botrytis cinerea and strong inhibition effect to Magnaporthe oryzae,meanwhile it shows obvious growth promotion effect.

引文

[1]张立新,宋江华.洋葱伯克氏菌群的多样性[J].中国农学报,2008,24(12):82-85.
    [2]PARKE J L,GURIAN-SCHERMAN D.Diversity of the Burkholderia cepacia complex and implications for risk assessment of biological control strains[J].Ann Rev Phytopathol,2001,39:225-258.
    [3]QUAN C S,ZHENG W,LIU Q,et al.Isolation and characterization of a novel Burkholderia cepacia with strong antifungal activity against Rhizoctonia solani[J].Appl Microbiol Biotechnol,2006,72(6):1276-1284.
    [4]SHARIFI-TEHRANI A,AHMADZADEH M,SARANI S,et al.Powder formulation of Burkholderia cepacia for control of rape seed damping-off caused by Rhizoctonia solani[J].Communications in Agricultre and Applied Biological Sciences,2007,72(2):129-136.
    [5]KOTAN R,FIKRETTIN S,ERKOL D,et al.Biological control of the potato dry rot caused by Fusarium species using PGPR strains[J].Biol Control,2009,50(2):194-198.
    [6]张立新,谢关林,罗远婵.洋葱伯克氏菌在农业上应用的利弊探讨[J].中国农业科学,2006,39(6):1166-1772.
    [7]MAHENTHIRALINGAM E,BALDWIN A,DOWSON C G.Burkholderia cepacia complex bacteria:opportunistic pathogens with important natural biology[J].J Appl Microbiol,2008,104:1539-1551.
    [8]BURKHOLDER W H.Sour skin,a bacterial rot of onion bulbs[J].Phytopathology,1950,40:115-117.
    [9]孙福在,赵廷昌.冰核细菌生物学特性及其诱发植物霜冻机理与防霜应用[J].生态学报,2003,23(2):336-344.
    [10]BURBAGE D A,SASSER M.A medium selective for Pseudomonas cepacia[J].Phytopathology,1982,72:706.
    [11]MAHENTHIRALINGAM E,BISCHOF J,BYRNE S K,et al.DNA-based diagnostic approaches for identification of Burkholderia cepacia complex,Burkholderia vietnamiensis,Burkholderia multivorans,Burkholderia stabilis,and Burkholderia cepacia genomovars I and III[J].J Clin Microbiol,2000,38:3165-3173.
    [12]ZHANG L X,XIE G L.Diversity and distribution of Burkholderia cepacia complex in the rhizosphere of rice and maize[J].FEMS Microbiol Lett,2007,266:231-235.
    [13]COENYE T,MAHENTHIRALINGAM E,HENRY D,et al.Burkholderia ambifaria sp.nov.,a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis related isolates[J].Int J Syst Evol Microbiol,2001,51:1481-1490.
    [14]RAMETTE A,LIPUMAI J J,TIEDJE J M.Species abundance and diversity of Burkholderia cepacia complex in the environment[J].Appl Environ Microbiol,2005,71(3):1193-1201.
    [15]VANLAERE E,HANSRAJ F,VANDAMME P A,et al.Growth in Stewart’s medium is a simple,rapid and inexpensive screening tool for the identification of Burkholderia cepacia complex[J].J Cyst Fibros,2006,5:137-139.
    [16]HERNANDEZ-RODRIGUEZ A,HEYDRICH-PEREZ M,DIALLO B,et al.Cell-free culture medium of Burkholderia cepacia improves seed germination and seedling growth in maize(Zea mays)and rice(Oryza sativa)[J].Plant Growth Regul,2010,60:191-197.