龙蛭汤对子痫前期模型大鼠胎盘灌注的影响
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摘要
目的:探究龙蛭汤(自拟方)对子痫前期模型大鼠胎盘灌注的影响。方法:选取60只6周龄SPF级SD大鼠体,质量200±30g,采用通过改良血管狭窄位置制作子痫前期大鼠模型,分为3组,空白对照组(control)、模型组(model),龙蛭汤组(LZT),分别检测大鼠孕后血压、24h尿蛋白含量变化;使用酶联免疫吸附法检测血浆IL-6、TNF-α浓度;使用硝酸还原酶法检测各组大鼠血浆NO浓度;使用Westen Bolt检测大鼠血管内皮生长因子(VEGF)、白细胞黏附分子(CD49d)、胎盘生长因子(PLGF)、可溶性血管内皮生长因子受体1(sFlt-1)和缺氧诱导因子(HIF-1)表达情况;结果:相比空白对照组,模型组大鼠血压、24h尿蛋白含量、IL-6、TNF-α浓度、CD49d、PLGF、sFlt-1、HIF-1表达显著升高,且差异有统计学意义(P<0.01),血浆NO浓度和VEGF表达量显著降低,且差异有统计学意义(P<0.01)。相比模型组大鼠,龙蛭汤组大鼠血压、24h尿蛋白含量、IL-6、TNF-α浓度、CD49d、PLGF、sFlt-1、HIF-1表达显著降低,且差异有统计学意义(P<0.01),血浆NO浓度和VEGF表达量显著升高,且差异有统计学意义(P<0.01)。结论:龙蛭汤通过降低大鼠血压、尿蛋白、炎症因子及胎盘组织相关因子的表达对子痫前期盘胎盘灌注血流不足有一定缓解疗效果。
Objective To explore the effects of Longzhi decoction(self-made formula) on placental perfusion in model rats with preeclampsia.Methods Sixty 6-week-old SPF SD rats were obtained with a mass of 200±30 g.The preeclampsia rat models were established by modifying the location of vascular stenosis,and were divided into three groups,including blank control group(control),model group(model) and Longzhi decoction group(LZT).The changes of blood pressure and 24 h urine protein were detected after pregnancy.The levels of plasma IL-6 and TNF-α were detected by enzyme-linked immunosorbent assay(ELISA).Plasma NO concentration was detected by nitrate reductase method.The expression levels of vascular endothelial growth factor(VEGF),leukocyte adhesion molecule(CD49 d),placental growth factor(PLGF),soluble vascular endothelial growth factor receptor 1(sFlt-1) and hypoxia-inducible factor(HIF-1) were detected by Westen Bolt.Results Compared with blank control group,the blood pressure,24 h urine protein content,IL-6,TNF-α concentration and expression levels of CD49 d,PLGF,sFlt-1 and HIF-1 in model group were significantly increased(P<0.01),and the plasma NO concentration and VEGF expression were decreased significantly(P<0.01).Compared with model group,the blood pressure,24 h urine protein content,IL-6,TNF-α concentration and expression levels of CD49 d,PLGF,sFlt-1 and HIF-1 in Longzhi decoction group were significantly decreased(P<0.01),and the plasma NO concentration and VEGF expression were increased significantly(P<0.01).Conclusions Longzhi decoction has a certain effect on the relief of insufficient blood flow of placental perfusion in preeclampsia through reducing the blood pressure,urine protein,inflammatory factors and placental tissue-related factors of rats.
Objective To explore the effects of Longzhi decoction(self-made formula) on placental perfusion in model rats with preeclampsia.Methods Sixty 6-week-old SPF SD rats were obtained with a mass of 200±30 g.The preeclampsia rat models were established by modifying the location of vascular stenosis,and were divided into three groups,including blank control group(control),model group(model) and Longzhi decoction group(LZT).The changes of blood pressure and 24 h urine protein were detected after pregnancy.The levels of plasma IL-6 and TNF-α were detected by enzyme-linked immunosorbent assay(ELISA).Plasma NO concentration was detected by nitrate reductase method.The expression levels of vascular endothelial growth factor(VEGF),leukocyte adhesion molecule(CD49 d),placental growth factor(PLGF),soluble vascular endothelial growth factor receptor 1(sFlt-1) and hypoxia-inducible factor(HIF-1) were detected by Westen Bolt.Results Compared with blank control group,the blood pressure,24 h urine protein content,IL-6,TNF-α concentration and expression levels of CD49 d,PLGF,sFlt-1 and HIF-1 in model group were significantly increased(P<0.01),and the plasma NO concentration and VEGF expression were decreased significantly(P<0.01).Compared with model group,the blood pressure,24 h urine protein content,IL-6,TNF-α concentration and expression levels of CD49 d,PLGF,sFlt-1 and HIF-1 in Longzhi decoction group were significantly decreased(P<0.01),and the plasma NO concentration and VEGF expression were increased significantly(P<0.01).Conclusions Longzhi decoction has a certain effect on the relief of insufficient blood flow of placental perfusion in preeclampsia through reducing the blood pressure,urine protein,inflammatory factors and placental tissue-related factors of rats.
引文
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[2] 靳艳霞,高劲松,胡静,等.sFlt-1/PlGF比值诊断子痫前期的研究进展[J].中华妇产科杂志,2016,51(7):548～550
[3] 董方华,杜丹,何栩,等.子痫前期患者血浆miRNA及相关凝血功能指标的相关性分析[J].广东医学,2016,37(16):2434～2436
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[6] 冒慧敏,史大卓.活血化瘀中药调节急性冠脉综合征炎症反应的临床研究进展[J].辽宁中医杂志,2016,3(1):213～216
[7] 王蔚,白雪,杨思进,等.蛭龙活血通瘀胶囊新旧工艺活血化瘀作用的比较研究[J].时珍国医国药,2016,6(2):367～369
[8] 沈小雅,潘石蕾,蔡蔚,等.孕期血糖逆向增高与妊娠结局的关系研究[J].解放军医学杂志,2017,42(1):47～51
[9] 赵洪波,王亚辉,周佳冰,等.尼古丁通过抑制可溶性血管内皮生长因子受体1促进胎盘生长因子分泌并提高人滋养细胞侵袭行为的分子机制[J].生殖与避孕,2016,36(7):547～551
[10] 李红,闫欢,朱琳琳,et al.早发重度 PE 及 HEELP 综合征患者胎盘组织、母血、脐血中 HMGB1和 RAGP 的表达观察[J].山东医药,2016,56(43):34～37
[11] CML慢性期患者血管VEGF、Flt-1的表达水平及其临床意义[J].医学临床研究,2016,33(8):1504～1507
[12] 史萌萌,奚胜艳,王彦晖,et al.桃红四物汤对肝纤维化小鼠病理性血管生成相关因子VEGF、KDR与Flt-1表达的影响[J].中华中医药杂志,2016,3(2):487～491
[13] 虞秋月.子痫前期患者胎盘组织PLGF表达及血清HGF和VEGF测定[J].放射免疫学杂志,2010,23(2):123～125
[14] 王靳琎,杨晓梅,苏丽万,等.海带多糖对脂多糖致慢性炎症大鼠血管内皮的保护作用[J].中国药理学通报,2018,8(5):651～656
[15] 张静.BISAP评分联合促炎/抗炎细胞因子检测对重症急性胰腺炎预后的评估价值[J].解放军医药杂志,2016,28(02):62～65+73
[16] 李晓燕,高艳凤,曹宏敏,等.白细胞介素-17家族细胞因子参与调控糖尿病大鼠肾脏炎症反应的相关性[J].中国老年学杂志,2017,37(18):112
[17] 马金昀,张若曦,程晓东.血清细胞因子IL-17与脱髓鞘性神经病多发性硬化相关性的研究:Meta分析[J].中国免疫学杂志,2017,33(12):1832～1837
[2] 靳艳霞,高劲松,胡静,等.sFlt-1/PlGF比值诊断子痫前期的研究进展[J].中华妇产科杂志,2016,51(7):548～550
[3] 董方华,杜丹,何栩,等.子痫前期患者血浆miRNA及相关凝血功能指标的相关性分析[J].广东医学,2016,37(16):2434～2436
[4] 胡晏馨,马润玫.妊娠期高血压疾病终止妊娠的时机[J].中国计划生育和妇产科,2016,8(5):9～11
[5] 李婷婷,黄为民.高危妊娠与早产胎盘病理缺氧性改变及新生儿结局的研究[J].中国妇幼保健,2016,31(17):3469～3471
[6] 冒慧敏,史大卓.活血化瘀中药调节急性冠脉综合征炎症反应的临床研究进展[J].辽宁中医杂志,2016,3(1):213～216
[7] 王蔚,白雪,杨思进,等.蛭龙活血通瘀胶囊新旧工艺活血化瘀作用的比较研究[J].时珍国医国药,2016,6(2):367～369
[8] 沈小雅,潘石蕾,蔡蔚,等.孕期血糖逆向增高与妊娠结局的关系研究[J].解放军医学杂志,2017,42(1):47～51
[9] 赵洪波,王亚辉,周佳冰,等.尼古丁通过抑制可溶性血管内皮生长因子受体1促进胎盘生长因子分泌并提高人滋养细胞侵袭行为的分子机制[J].生殖与避孕,2016,36(7):547～551
[10] 李红,闫欢,朱琳琳,et al.早发重度 PE 及 HEELP 综合征患者胎盘组织、母血、脐血中 HMGB1和 RAGP 的表达观察[J].山东医药,2016,56(43):34～37
[11] CML慢性期患者血管VEGF、Flt-1的表达水平及其临床意义[J].医学临床研究,2016,33(8):1504～1507
[12] 史萌萌,奚胜艳,王彦晖,et al.桃红四物汤对肝纤维化小鼠病理性血管生成相关因子VEGF、KDR与Flt-1表达的影响[J].中华中医药杂志,2016,3(2):487～491
[13] 虞秋月.子痫前期患者胎盘组织PLGF表达及血清HGF和VEGF测定[J].放射免疫学杂志,2010,23(2):123～125
[14] 王靳琎,杨晓梅,苏丽万,等.海带多糖对脂多糖致慢性炎症大鼠血管内皮的保护作用[J].中国药理学通报,2018,8(5):651～656
[15] 张静.BISAP评分联合促炎/抗炎细胞因子检测对重症急性胰腺炎预后的评估价值[J].解放军医药杂志,2016,28(02):62～65+73
[16] 李晓燕,高艳凤,曹宏敏,等.白细胞介素-17家族细胞因子参与调控糖尿病大鼠肾脏炎症反应的相关性[J].中国老年学杂志,2017,37(18):112
[17] 马金昀,张若曦,程晓东.血清细胞因子IL-17与脱髓鞘性神经病多发性硬化相关性的研究:Meta分析[J].中国免疫学杂志,2017,33(12):1832～1837