HPLC同时测定鹿衔草提取物中香草酸、金丝桃苷、槲皮素-3-O-呋喃阿拉伯糖苷、槲皮素和梅笠草素的含量
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摘要
目的:建立HPLC同时测定鹿衔草提取物中香草酸、金丝桃苷、槲皮素-3-O-呋喃阿拉伯糖苷、槲皮素和梅笠草素的含量。方法:色谱柱:Zorbax SB-C_(18)(250 mm×4.6 mm,5μm)柱,流动相:乙腈-0.1%醋酸水,梯度洗脱;柱温:35℃,流速:1.0 ml·min~(-1),检测波长:255 nm。结果:香草酸、金丝桃苷、槲皮素-3-O-呋喃阿拉伯糖苷、槲皮素和梅笠草素分别在3.71～148.40μg·ml~(-1)(r=0.999 9)、5.92～236.70μg·ml~(-1)(r=0.999 8)、3.36～134.25μg·ml~(-1)(r=0.999 8)、3.94～157.80μg·ml~(-1)(r=0.999 9)和3.10～124.15μg·ml~(-1)(r=0.999 8)范围内线性关系良好,平均回收率分别为99.01%、98.80%、98.48%、98.71%和98.62%,RSD分别为0.95%、1.15%、1.36%、0.94%和1.02%(n=6)。结论:该方法快捷简便、准确性及重复性均良好,可用于鹿衔草提取物及相关制剂质量的评价和控制。
Objective:To set up an HPLC method for the simultaneous determination of vanillic acid,hyperin,quercetin-3-O-furanoarabinoside,quercetin and chimaphilin in the extracts of Pyrolae Herba.Methods:A Zorbax SB-C_(18)column(250 mm×4.6 mm,5μm)was used and use mobile phase was acetonitrile-0.1%acetic acid with gradient elution at a flow rate of 1.0 ml·min~(-1).The column temperature was 35℃.The detection wavelength was 255 nm.Results:Good linear relationships were found within the range of 3.71-148.40μg·ml~(-1)(r=0.999 9)for vanillic acid,5.92-236.70μg·ml~(-1)(r=0.999 8)for hyperin,3.36-134.25μg·ml~(-1)(r=0.999 8)for quercetin-3-O-furanoarabinoside,3.94-157.80μg·ml~(-1)(r=0.999 9)for quercetin and 3.10-124.15μg·ml~(-1)(r=0.999 8)for chimaphilin.The average recovery was 99.01%(RSD=0.95%),98.80%(RSD=1.15%),98.48%(RSD=1.36%),98.71%(RSD=0.94%)and 98.62%(RSD=1.02%)(n=6),respectively.Conclusion:The method is rapid,simple,accurate and reproducible,and can be used for the quality evaluation and control of Pyrolae Herba extracts and the related preparations.
Objective:To set up an HPLC method for the simultaneous determination of vanillic acid,hyperin,quercetin-3-O-furanoarabinoside,quercetin and chimaphilin in the extracts of Pyrolae Herba.Methods:A Zorbax SB-C_(18)column(250 mm×4.6 mm,5μm)was used and use mobile phase was acetonitrile-0.1%acetic acid with gradient elution at a flow rate of 1.0 ml·min~(-1).The column temperature was 35℃.The detection wavelength was 255 nm.Results:Good linear relationships were found within the range of 3.71-148.40μg·ml~(-1)(r=0.999 9)for vanillic acid,5.92-236.70μg·ml~(-1)(r=0.999 8)for hyperin,3.36-134.25μg·ml~(-1)(r=0.999 8)for quercetin-3-O-furanoarabinoside,3.94-157.80μg·ml~(-1)(r=0.999 9)for quercetin and 3.10-124.15μg·ml~(-1)(r=0.999 8)for chimaphilin.The average recovery was 99.01%(RSD=0.95%),98.80%(RSD=1.15%),98.48%(RSD=1.36%),98.71%(RSD=0.94%)and 98.62%(RSD=1.02%)(n=6),respectively.Conclusion:The method is rapid,simple,accurate and reproducible,and can be used for the quality evaluation and control of Pyrolae Herba extracts and the related preparations.
引文
1中国药典[S].2015年版.一部.324
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5蔡亮亮,余方荣,陈加守,等.鹿衔草挥发油诱导人软骨肉瘤细胞SW1353凋亡的机制[J].中华中医药杂志,2014,29(4):1085-1088
6 Yao XH,Zhang DY,Luo M,et al.Negative pressure cavitation-microwave assisted preparation of extract of Pyrola incarnata Fisch.rich in hyperin,2'-O-galloylhyperin and chimaphilin and evaluation of its antioxidant activity[J].Food Chem,2015,169:270-276
7王丹阳.鹿蹄草提取物的抗氧化活性及抑菌性的研究[D].陕西杨凌:西北农林科技大学硕士学位论文,2014
8李超雄,吴银生,林向全,等.鹿衔草水提剂对去卵巢模鼠骨代谢的影响[J].福建医药杂志,2016,38(4):49-51
2国家中医药管理局《中华本草》编委会.中华本草(第6册)[M].上海:上海科学技术出版社,1999:6-9
3赵泽丰,吴妮,田雪,等.鹿蹄草属植物化学成分、药理活性与质量控制研究进展[J].中国中药杂志,2017,42(4):618-627
4褚颖.鹿衔草中梅笠草素对脑缺血性大鼠的保护作用及机制研究[D].长春:长春中医药大学博士学位论文,2017
5蔡亮亮,余方荣,陈加守,等.鹿衔草挥发油诱导人软骨肉瘤细胞SW1353凋亡的机制[J].中华中医药杂志,2014,29(4):1085-1088
6 Yao XH,Zhang DY,Luo M,et al.Negative pressure cavitation-microwave assisted preparation of extract of Pyrola incarnata Fisch.rich in hyperin,2'-O-galloylhyperin and chimaphilin and evaluation of its antioxidant activity[J].Food Chem,2015,169:270-276
7王丹阳.鹿蹄草提取物的抗氧化活性及抑菌性的研究[D].陕西杨凌:西北农林科技大学硕士学位论文,2014
8李超雄,吴银生,林向全,等.鹿衔草水提剂对去卵巢模鼠骨代谢的影响[J].福建医药杂志,2016,38(4):49-51
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