三种生长调节剂对青檀硬枝扦插生根的影响
|
摘要
采用青檀幼树上的一二年生枝条为材料,运用响应曲面法,以平均生根率、平均根重、平均生长枝条数、根平均长度、根平均投影面积、根平均表面积、根平均体积和根平均直径为指标,探讨不同浓度的生长调节剂IAA、NAA、IBA和浸泡时间对青檀硬枝扦插生根的影响。各指标的相关分析表明,插穗着生的枝条数与生根率存在着显著正相关关系,可作为生根率的直观判断指标;模型模拟及验证试验结果表明,青檀硬枝扦插生根促进剂的最佳组合为:IAA 250mg/L、IBA 250mg/L、NAA 750mg/L,浸泡时间为30min,生根率为62.5%。
By using the Response Surface Methodology,with the average rooting rate,average root weight,average growth branch number,root average length,root average area,root average surface area,root average volume and root average diameter as observation indices,the effects of different concentrations of growth regulators IAA,NAA,IBA and soaking time on the rooting of hardwood cuttings were examined,using annual branches and biennial branches as materials.Correlation analysis between different indices showed that there was a significant positive correlation between the number of branches and the rooting rate,which could be used as a direct index for determining the rooting rate.The model simulation and test results showed that the best combination of the rooting promoter was IAA 250 mg/L,IBA 250 mg/L,NAA 750 mg/L.In addition,the soaking time was 30 min and the rooting rate was 62.5%.
By using the Response Surface Methodology,with the average rooting rate,average root weight,average growth branch number,root average length,root average area,root average surface area,root average volume and root average diameter as observation indices,the effects of different concentrations of growth regulators IAA,NAA,IBA and soaking time on the rooting of hardwood cuttings were examined,using annual branches and biennial branches as materials.Correlation analysis between different indices showed that there was a significant positive correlation between the number of branches and the rooting rate,which could be used as a direct index for determining the rooting rate.The model simulation and test results showed that the best combination of the rooting promoter was IAA 250 mg/L,IBA 250 mg/L,NAA 750 mg/L.In addition,the soaking time was 30 min and the rooting rate was 62.5%.
引文
[1]中国科学院中国植物志编辑委员会.中国植物志:22卷[M].北京:科学出版社,1998:380-382.
[2]李正理,胡玉熹,刘淑琼.青檀各年枝韧皮纤维的比较解剖[J].植物学报,1965,13(4):330-338.
[3]LI X H,SHAO J W,LU G,et al..Chloroplast phylogeography of a temperate tree Pteroceltis tatarinowii(Ulmaceae)in China[J].Journal of Systematics and Evolution,2012,50(4):325-333.
[4]方升佐,崔同林,虞木奎.成土母岩和条龄对青檀檀皮质量的影响[J].北京林业大学学报,2007,29(2):122-127.
[5]FANG S Z,SONG L Y,FU X X.Effects of NaCl stress on seed germination,leaf gas exchange and seedling growth of Pteroceltis tatarinowii[J].Journal of Forestry Research,2006,17(3):185-188.
[6]王洪强,王芬,王孟军,等.青檀优良无性系选育[J].中国园艺文摘,2013(6):14-16.
[7]苏治南,陈诗文,杨家鸿,等.不同生根剂对杉木扦插根形态建成的影响[J].基因组学与应用生物学,2013,32(6):787-794.
[8]孙铭浩,王加彬,王芬,等.青檀优良夫性系TX01嫁接育苗试验[J].林业科技通讯,2016(6):29-31.
[9]王因花,刘翠兰,王开芳,等.青檀嫩枝扦插繁殖技术研究[J].山东农业科学,2016,48(10):74-77.
[10]王峰,张靖,陈荣伟,等.青檀嫩枝扦插技术研究[J].园艺与种苗,2015(9):18-21.
[11]卫春华,王鸣凤.青檀嫩枝扦插育苗技术[J].安徽林业科技,2005(3):44-45.
[12]李朝晖.青檀嫩枝扦插技术及不定根形成过程中生理生化动态研究[D].泰安:山东农业大学,2014.
[13]李红卫,赵庆涛,仝太伟.青檀硬枝扦插育苗技术[J].河南林业科技,2011,31(2):53-54.
[14]孙铭浩,李正伟,刘文涛,等.青檀新品种‘宏选1号’硬枝扦插育苗试验[J].中国园艺文摘,2017(2):11-13,29.
[2]李正理,胡玉熹,刘淑琼.青檀各年枝韧皮纤维的比较解剖[J].植物学报,1965,13(4):330-338.
[3]LI X H,SHAO J W,LU G,et al..Chloroplast phylogeography of a temperate tree Pteroceltis tatarinowii(Ulmaceae)in China[J].Journal of Systematics and Evolution,2012,50(4):325-333.
[4]方升佐,崔同林,虞木奎.成土母岩和条龄对青檀檀皮质量的影响[J].北京林业大学学报,2007,29(2):122-127.
[5]FANG S Z,SONG L Y,FU X X.Effects of NaCl stress on seed germination,leaf gas exchange and seedling growth of Pteroceltis tatarinowii[J].Journal of Forestry Research,2006,17(3):185-188.
[6]王洪强,王芬,王孟军,等.青檀优良无性系选育[J].中国园艺文摘,2013(6):14-16.
[7]苏治南,陈诗文,杨家鸿,等.不同生根剂对杉木扦插根形态建成的影响[J].基因组学与应用生物学,2013,32(6):787-794.
[8]孙铭浩,王加彬,王芬,等.青檀优良夫性系TX01嫁接育苗试验[J].林业科技通讯,2016(6):29-31.
[9]王因花,刘翠兰,王开芳,等.青檀嫩枝扦插繁殖技术研究[J].山东农业科学,2016,48(10):74-77.
[10]王峰,张靖,陈荣伟,等.青檀嫩枝扦插技术研究[J].园艺与种苗,2015(9):18-21.
[11]卫春华,王鸣凤.青檀嫩枝扦插育苗技术[J].安徽林业科技,2005(3):44-45.
[12]李朝晖.青檀嫩枝扦插技术及不定根形成过程中生理生化动态研究[D].泰安:山东农业大学,2014.
[13]李红卫,赵庆涛,仝太伟.青檀硬枝扦插育苗技术[J].河南林业科技,2011,31(2):53-54.
[14]孙铭浩,李正伟,刘文涛,等.青檀新品种‘宏选1号’硬枝扦插育苗试验[J].中国园艺文摘,2017(2):11-13,29.