响应面法优化黄连-罗汉果药对中降糖成分的超声提取工艺研究
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摘要
本文优化了黄连-罗汉果药对中发挥降血糖作用的主要指标性有效成分小檗碱型生物碱、多糖和罗汉果皂苷的超声提取工艺。利用响应面法考察超声提取时间、料液比例、超声温度与超声功率对提取效果的影响。最佳提取条件为将黄连、罗汉果细粉浸泡于1∶30倍量的水中,置于75℃、400 W的恒温超声仪中提取23 min,提取3次,得到的提取液中三种指标成分质量总和最高,其中小檗碱型生物碱、多糖和罗汉果皂苷的提取率分别为22.70%、31.16%、16.55%。该提取工艺简单高效,预测性良好,有利于促进黄连、罗汉果合用发挥降血糖功效的开发利用。
The ultrasonic-assisted extraction process of berberine alkaloids,polysaccharidesand siraitia grosvenorii saponins,which were the main indicators of the hypoglycemic effect of Rhizoma coptidis-Siraitia grosvenorii compound,was optimized.Response surface method was used to study the effects of ultrasonic time,solid-liquid ratio,ultrasonic temperature and ultrasonic power on extraction efficiency.The optimal conditions were as follows:solid-liquid ratio 1∶30,ultrasonic temperature 75 ℃,ultrasonic power 400 W,ultrasonic time23 min,extraction frequency 3 times.Under the optimal conditions,the total content of the three indexes in the extracted liquid was the highest,with the extraction ratio of berberine alkaloids,polysaccharidesand siraitia grosvenorii saponins at 22.70%,31.16%and 16.55%respectively.The Box-Behnken response surface method could be used for preparing production with high accuracy.The extraction process was simple,efficient and predictive,which was conducive to the development and utilization of the hypoglycemic effect of Rhizoma coptidis and Siraitia grosvenorii.
The ultrasonic-assisted extraction process of berberine alkaloids,polysaccharidesand siraitia grosvenorii saponins,which were the main indicators of the hypoglycemic effect of Rhizoma coptidis-Siraitia grosvenorii compound,was optimized.Response surface method was used to study the effects of ultrasonic time,solid-liquid ratio,ultrasonic temperature and ultrasonic power on extraction efficiency.The optimal conditions were as follows:solid-liquid ratio 1∶30,ultrasonic temperature 75 ℃,ultrasonic power 400 W,ultrasonic time23 min,extraction frequency 3 times.Under the optimal conditions,the total content of the three indexes in the extracted liquid was the highest,with the extraction ratio of berberine alkaloids,polysaccharidesand siraitia grosvenorii saponins at 22.70%,31.16%and 16.55%respectively.The Box-Behnken response surface method could be used for preparing production with high accuracy.The extraction process was simple,efficient and predictive,which was conducive to the development and utilization of the hypoglycemic effect of Rhizoma coptidis and Siraitia grosvenorii.
引文
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[15]黄志强,周民杰,毛献萍.黄连中小檗碱的超声波提取工艺及数学模拟[J].应用化学,2006,35(7):543-544.
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[2]吕春霞.黄连的古今药用价值及临床研究[J].中医中药,2013,8(22):341.
[3]吴玉娟,王懿萍,姜延伟,等.黄连多糖含量测定及抗氧化活性研究[J].时珍国医国药,2008,(8):1 906-1 908.
[4]陈洪源,明智强,谢佳乐,等.小檗碱降血糖机制的研究进展[J].食品与药品,2008,10(3):69-71.
[5]万艳娟,吴军林,吴清平.罗汉果降血糖作用及机理研究进展[J].食品研究与开发,2016,36(11):188-191.
[6]白玉鹏,李娜,顾晔.罗汉果皂苷提取物对实验性糖尿病大鼠的血管保护作用及机制[J].上海医学,2009,32(5):400-405.
[7]Song F,Qi X,Chen W,et a1.Effect of Momordicagrosvenori on oxidative srtess pahtways in renal mitochondria of normal andalloxan-induced diabetic mice:in volvementofheme oxygenase-l[J].Eur J Nutr,2007,46(2):61-69.
[8]何超文,文君,朱晓韵,等.鲜罗汉果素急性毒性安全性评价研究[J].广西轻工业,2011,(8):4-5.
[9]邱超,崔元璐,齐学洁,等.黄连和吴茱萸配伍现代研究进展[J].中国中药杂志,2015,40(4):582-587.
[10]陈建平,于姝燕,郭慧卿,等.响应面法优化蒙药瞿麦总酚提取工艺研究及含量测定[J].化学研究与应用,2018,30(7):1086-1094.
[11]彭瑾,张蓉,彭梅.紫外-可见分光光度法测定不同产地罗汉果中多糖含量[J].中国药业,2016,25(2):51-53.
[12]梁宇锋,林永春.分光光度法测定罗汉降糖颗粒中罗汉果甜苷含量[J].牡丹江医学院学报,2009,30(2):37-38.
[13]何超文,姚美村,朱晓韵,等.鲜罗汉果皂苷提取工艺及其成分分析[J].广西轻工业,2011,(12):1-2.
[14]张海容,白娟,魏増云,等.超声萃取-响应面法优化淡竹叶多糖提取方法研究[J].化学研究与应用,2013,25(3):303-310.
[15]黄志强,周民杰,毛献萍.黄连中小檗碱的超声波提取工艺及数学模拟[J].应用化学,2006,35(7):543-544.
[16]刘静,荣永海,王志滨,等.罗汉果有效成分的连续提取和分离[J].中药材,2010,33(4):629-631.