20%吡唑醚菌酯·丙硫菌唑悬浮剂的高效液相色谱分析
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摘要
建立了20%吡唑醚菌酯·丙硫菌唑悬浮剂高效液相色谱测定方法。采用Eclipse XDB-C_(18)反相柱和紫外可变波长检测器,以乙腈+水+冰乙酸为流动相,在260 nm波长下同时测定试样中吡唑醚菌酯和丙硫菌唑的含量。结果表明,吡唑醚菌酯和丙硫菌唑的线性相关系数分别为1.000 0和0.999 9,标准偏差分别为0.047和0.054,变异系数分别为0.45%和0.52%,平均回收率分别为99.72%和99.85%。该方法操作简便,精密度与准确度较高。
The high performance liquid chromatography method for the determination of active ingredient content in pyraclostrobin + prothioconazole 20% SC was established. The simultaneous determination of pyraclostrobin and prothioconazole in the samples was conducted with Eclipse XDB-C_(18) column and UV-absobance detector at 260 nm, while the mixture solution of acetonitrile, water and acetic acid was used as the mobile phase. The results showed that the linear correlation coefficients of pyraclostrobin and prothioconazole were 1.000 0 and 0.999 9, the standard deviations were 0.047 and 0.054, the variation coefficients were 0.45% and 0.52%, and average recoveries were 99.72% and 99.85%,respectively. The method was a suitable analytical method with fast speed, high precision and accuracy.
The high performance liquid chromatography method for the determination of active ingredient content in pyraclostrobin + prothioconazole 20% SC was established. The simultaneous determination of pyraclostrobin and prothioconazole in the samples was conducted with Eclipse XDB-C_(18) column and UV-absobance detector at 260 nm, while the mixture solution of acetonitrile, water and acetic acid was used as the mobile phase. The results showed that the linear correlation coefficients of pyraclostrobin and prothioconazole were 1.000 0 and 0.999 9, the standard deviations were 0.047 and 0.054, the variation coefficients were 0.45% and 0.52%, and average recoveries were 99.72% and 99.85%,respectively. The method was a suitable analytical method with fast speed, high precision and accuracy.
引文
[1]左文静,主艳飞,庄占兴,等.吡唑醚菌酯研究开发现状与展望[J].世界农药, 2017, 39(1):22-25.
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[3]姚瑛,陈九星,李涛,等.吡唑醚菌酯悬浮剂的气相色谱法分析[J].精细化工中间体, 2013, 43(3):65-67; 72.
[4]郭利丰,肖鸣,黄雅俊,等. 38%唑醚·啶酰菌悬浮剂的高效液相色谱法分析[J].农药, 2016, 55(11):806-807.
[5]张万昌. 40%丙硫菌唑悬浮剂的高效液相色谱测定方法[J].现代农药, 2016, 15(4):36-37.
[6]刘淑杰,孙克,张敏恒.丙硫菌唑分析方法述评[J].农药, 2014, 53(9):699-701.
[2]关爱莹,李林,刘长令.新型三唑硫酮类杀菌剂丙硫菌唑[J].农药, 2003, 42(9):42-43; 41.
[3]姚瑛,陈九星,李涛,等.吡唑醚菌酯悬浮剂的气相色谱法分析[J].精细化工中间体, 2013, 43(3):65-67; 72.
[4]郭利丰,肖鸣,黄雅俊,等. 38%唑醚·啶酰菌悬浮剂的高效液相色谱法分析[J].农药, 2016, 55(11):806-807.
[5]张万昌. 40%丙硫菌唑悬浮剂的高效液相色谱测定方法[J].现代农药, 2016, 15(4):36-37.
[6]刘淑杰,孙克,张敏恒.丙硫菌唑分析方法述评[J].农药, 2014, 53(9):699-701.