UV-B辐射对孔石莼孢子生长发育的影响
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摘要
运用实验生态学的方法,研究了UV-B辐射对孔石莼孢子生长发育的影响,结果表明:孔石莼孢子发育早期外形主要呈现棒状和蝌蚪状,其中棒状占90.68%±2.50%。UV-B辐射对孔石莼孢子生长发育有明显的抑制作用,而且随着辐射剂量的增加,抑制作用愈加明显。在30 d时,受到不同剂量UV-B辐射的孔石莼幼苗均出现了死亡,死亡率随着UV-B辐射剂量的增加而升高。UV-B辐射会使孔石莼幼苗叶绿素a含量明显下降,而丙二醛含量会有不同程度的升高,因此细胞分裂受阻,叶绿素和细胞膜系统受到破坏是孢子生长发育受到抑制和幼苗死亡的原因之一。
The effects of UV-B radiation on the growth of Ulva pertusa Kjellman spores were studied by experimental ecological methods. The results showed that the growth morphology of Ulva pertusa Kjellman spores was mainly rod shape and tadpole shape. The percentage of rod shape was 90. 68% ± 2. 50%. UV-B radiation could inhibit the growth of Ulva pertusa Kjellman spores,and the growth inhibition was more significant with the increased doses of UV-B radiation treatment. On the day 30,Ulva pertusa Kjellman seedling had emerged death under UV-B radiation,and the death rate was increased with the increased doses of UV-B radiation treatment. The chlorophyll a contents of Ulva pertusa Kjellman seedling were significantly decreased,while MDA contents were increased by UV-B radiation. Therefore,the reasons of Ulva pertusa Kjellman seedling growth inhibition and death were cell division,chlorophyll and cell membrane system damage.
The effects of UV-B radiation on the growth of Ulva pertusa Kjellman spores were studied by experimental ecological methods. The results showed that the growth morphology of Ulva pertusa Kjellman spores was mainly rod shape and tadpole shape. The percentage of rod shape was 90. 68% ± 2. 50%. UV-B radiation could inhibit the growth of Ulva pertusa Kjellman spores,and the growth inhibition was more significant with the increased doses of UV-B radiation treatment. On the day 30,Ulva pertusa Kjellman seedling had emerged death under UV-B radiation,and the death rate was increased with the increased doses of UV-B radiation treatment. The chlorophyll a contents of Ulva pertusa Kjellman seedling were significantly decreased,while MDA contents were increased by UV-B radiation. Therefore,the reasons of Ulva pertusa Kjellman seedling growth inhibition and death were cell division,chlorophyll and cell membrane system damage.
引文
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[2]XU J,GAO K.UV-A enhanced growth and UV-B induced positive effects in the recovery of photochemical yield in Gracilaria lemaneiformis(Rhodophyta)[J].Journal of Photochemistry and Photobiology B:Biology,2010,100(3):117-122.
[3]RHEE J S,KIM B M,CHOI B S,et al.Expression pattern analysis of DNA repair-related and DNA damage response genes revealed by 55 K oligomicroarray upon UV-B irradiation in the intertidal copepod,Tigriopus japonicas[J].Comparative Biochemistry and Physiology Part C:Toxicology and Pharmacology,2012,155(2):359-368.
[4]SUBRAMANI P A,HAMEED B,MICHAEL R D.Effect of UV-B radiation on the antibody response of fish-implication on high altitude fish culture[J].Journal of Photochemistry and Photobiology B:Biology,2015,143:1-4.
[5]RIZZINI L,FAVORY J J,CLOIX C,et al.Perception of UV-B by the Arabidopsis UVR8 protein[J].Science,2011,332(6025):103-106.
[6]HEIJDE M,ULM R.UV-B photoreceptor-mediated signaling in plants[J].Trends in Plant Science,2012,17(4):230-237.
[7]苏秀榕,李太武,常少杰.孔石莼营养成份研究[J].中国海洋药物,1997(1):33-35.
[8]殷旭旺,李文香,白海锋,等.不同海藻饲料对刺参幼参生长的影响[J].大连海洋大学学报,2015,30(3):276-280.
[9]吕冬伟,刘峰,李兴佐.大型海藻孔石莼在工厂海水养殖中的生物修复及其应用前景[J].海洋科学,2012,36(12):95-98.
[10]蔡恒江,唐学玺.孔石莼和赤潮异弯藻相互作用的初步研究[J].大连海洋大学学报,2012,27(3):255-259.
[11]董开升.孔石莼和鼠尾藻对UV-B辐射的生理化响应[D].青岛:中国海洋大学,2008.
[12]HAN T,HAN Y S,KIM K Y,et al.Influences of light and UV-B on growth and sporulation of the green alga Ulva pertusa Kjellman[J].Journal of Experimental Marine Biology and Ecology,2003,290(1):115-131.
[13]GUILLARD R R,RYTHER J H.Studies on marine planktonic diatoms I.Cyclotella nana(Hustedt)and Detonula confervanceae(Cleve)Gran[J].Canadian Journal Microbiology,1962,8:229-239.
[14]JEFFERY S W,HUMPHERY G F.New spectrophotometric equations for determining chlorophyll a,b,c1 and c2 in higher plant,algae and natural phytoplankton[J].Plant Physiology&Biochemistry,1975,8:53-59.
[15]DHINDSA R S,MATOWE W.Drought tolerance in two mosses:correlated with enzymatic defense against lipid peroxidation[J].Journal of Experimental Botany,1981,32(1):19-91.
[16]GROBE C W,MURPHY T M.Solar ultraviolet-B radiation effects on growth and pigment composition of the intertidal alga Ulva expansa(setch)S.&G.(chlorophyta)[J].Journal of Experimental Marine Biology&Ecology,1998,225(1):39-51.
[17]赵天宏,刘轶鸥,王岩,等.O3浓度升高和UV-B辐射增强对大豆叶片叶绿素含量和活性氧代谢的影响[J].应用生态学报,2013,24(5):1277-1283.
[18]YAO Y,XUAN Z,LI Y,et al.Effects of ultraviolent-B radiation on crop growth,development,yield and leaf pigment concentration of tartary buckwheat(Fagopyrum tataricum)under field conditions[J].European Journal of Agronomy,2006,25(2):215-222.
[19]KARSTEN U,LEMBCKE S,SCHUMANN R.The effects of ultraviolet radiation on photosynthetic performance,growth and sunscreen compounds in aeroterrestrial biofilm algae isolated from building facades[J].Planta,2007,225(4):991 1000.
[20]蔡恒江,唐学玺,张培玉,等.UV-B辐射对青岛大扁藻生长及其某些生理特性的影响[J].海洋科学进展,2005,23(4):460-465.
[21]汪月霞,孙国荣,王建波,等.Na Cl胁迫下星星草幼苗MDA含量与膜透性及叶绿素荧光参数之间的关系[J].生态学报,2006,26(1):122-129.
[22]BOWLER C,VAN MONTAGU T,INZE D.Superoxide dismutase and stress tolerance[J].Annual Review of Plant Physiology&Plant Molecular Biology,1992,43:83-116.