白边大叶蝉线粒体基因组测序与分析
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摘要
采用引物步移法测得白边大叶蝉Kolla paulula(Walker)线粒体基因组90%左右的序列,并分析了该叶蝉的线粒体基因组特征。基于23个物种(半翅目)的蛋白编码基因序列,以最大似然法和贝叶斯法构建了头喙亚目系统发育树。已测得序列长度为13 579 bp(AT:73.33%),其中包含了13个蛋白编码基因、21个t RNA基因和1个r RNA基因。除了ND5基因使用GTT作为起始密码子外,其他所有蛋白编码基因均使用ATN作为起始密码子;除了CO II使用不完整的T作为终止密码子,其他所有蛋白编码基因均使用TAA或TAG作为终止密码子。21个t RNA基因中,除了t RNASer(AGN)缺失1个稳定的茎环结构外,其他所有t RNA基因均能形成典型的三叶草二级结构。系统发育分析结果表明,进化树明显可以被分为沫蝉总科+(角蝉总科+蜡蝉总科);白边大叶蝉属于角蝉总科的叶蝉科,白边大叶蝉线粒体基因组特征与其他叶蝉科昆虫相同。
About 90% of the mitochondrial genome of Kolla paulula(Walker) was sequenced using the primer extraction step method and the mitochondrial genomic characteristics was analyzed in this study. We constructed a phylogenetic tree from 23 species(Hemiptera)based on the nucleotide sequence of 13 mitochondrial protein-coding genes using Bayesian inference and Maximum likelihood. The sequence length was 13579 bp(AT: 73.33%), including 13 protein coding genes, 21 t RNA genes and 1 r RNA gene. All protein coding genes used ATN as initiation codon except ND5 that used GTT as initiation codon. All protein coding genes used TAA or TAG as stop codon except CO II that used incomplete T as stop codon. All of the t RNA genes formed a typical clover secondary structure except t RNASer(AGN) that was lack of a stable stem-loop structure among the 21 t RNA genes. The phylogenetic analysis results showed that the phylogenetic tree could be divided into Cercopoidea+(Membracoidea+ Fulgoroidea). The mitochondrial genome of Kolla paulula(Membracoidea: Cicadellidae)had the same characteristics as other Cicadellidae.
About 90% of the mitochondrial genome of Kolla paulula(Walker) was sequenced using the primer extraction step method and the mitochondrial genomic characteristics was analyzed in this study. We constructed a phylogenetic tree from 23 species(Hemiptera)based on the nucleotide sequence of 13 mitochondrial protein-coding genes using Bayesian inference and Maximum likelihood. The sequence length was 13579 bp(AT: 73.33%), including 13 protein coding genes, 21 t RNA genes and 1 r RNA gene. All protein coding genes used ATN as initiation codon except ND5 that used GTT as initiation codon. All protein coding genes used TAA or TAG as stop codon except CO II that used incomplete T as stop codon. All of the t RNA genes formed a typical clover secondary structure except t RNASer(AGN) that was lack of a stable stem-loop structure among the 21 t RNA genes. The phylogenetic analysis results showed that the phylogenetic tree could be divided into Cercopoidea+(Membracoidea+ Fulgoroidea). The mitochondrial genome of Kolla paulula(Membracoidea: Cicadellidae)had the same characteristics as other Cicadellidae.
引文
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[24]SONG N,LIANG A P.The complete mitochondrial genome sequence of Geisha distinctissima(Hemiptera:Flatidae)and comparison with other hemipteran insects[J].Acta Biochim Biophys Sin,2009,41(3):206-216.
[25]ZHOU N,WANG M,CUI L,et al.Complete mitochondrial genome of Empoasca vitis(Hemiptera:Cicadellidae)[J].Mitochondrial DNA Part A,2016,27(2):1052-1053.
[26]LIANG A P,GAO J,ZHAO X.Characterization of the complete mitochondrial genome of the treehopper Darthula hardwickii(Hemiptera:Aetalionidae)[J].Mitochondrial DNA Part A,2016,27(5):3291-3292.
[27]WU Y,DAI R,ZHAN H,et al.Complete mitochondrial genome of Drabescoides nuchalis(Hemiptera:Cicadellidae)[J].Mitochondrial DNA A,2016,27(5):3626-3627.
[28]ZHAO X,LIANG A P.Complete DNA sequence of the mitochondrial genome of the treehopper Leptobelus gazella(Membracoidea:Hemiptera)[J].Mitochondrial DNA Part A,2016,27(5):3318-3319.
[29]HUNNICUTT L E,HUNTER W B,MOZORUK J J.The complete mitochondrial genome of the blue-green sharpshooter,Graphocephala atropunctata(Hemiptera:Cicadellidae)[C]//Entomological Society of America Meeting.Reno:2008.
[30]YANG H,LIU J,LIANG A.The complete mitochondrial genome of Cosmoscarata bispecularis(Hemiptera,Cicadomorpha,Cercopoidea,Cercopidae)[J].Mitochondrial DNA Part A,2016,27(6):3957-3958.
[31]牛芳芳,陈静,姜立云,等.两种毛蚜线粒体基因组比较分析[J].应用昆虫学报,2016,53(6):1288-1300.
[2]郭仲龙,袁明龙.半翅目昆虫线粒体基因组学研究进展[J].中国科学:生命科学,2016(2):151-166.
[3]ZHONG H,ZHANG Y,WEI C.Morphology and ultra-structure of the malpighian tubules in Kolla paulula,(Hemiptera:Cicadellidae)[J].Zool Anz,2015,257:22-28.
[4]张熏尹.黑尾大叶蝉(Bothrogonia ferruginea)生活史及媒介葡萄皮尔斯病之传播效率探讨[D].台中:中兴大学,2014.
[5]TUAN S J,HU F T,CHANG H Y,et al.Xylella fastidiosa transmission and life history of two Cicadellinae sharpshooters,Kolla paulula and Bothrogonia ferruginea(Hemiptera:Cicadellidae),in Taiwan[J].J Econ Entomol,2016,109(3):1034-1040.
[6]CREAMER R,CARPENTER J,RASCON J.Incidence of the beet leafhopper,Circulifer tenellus(Homoptera:Cicadellidae)in New Mexico chile[J].Southwest Entomol,2003,28(3):177-182.
[7]葛钟麟.中国经济昆虫志:第10册,同翅目:叶蝉科[M].北京:科学出版社,1966:60-62.
[8]METCALF Z P.General catalogue of the Homoptera.Fascicle VI,Cicadelloidea.Part 1,Tettigellidae[M].Washington D C:United States Department of Agriculture,1965.
[9]CARVER M,GROSS G F,WOODWARD T E.Hemiptera(bugs,leafhoppers,cicadas,aphids,scale insects etc.)[J].The insects of Australia,1991,1:429-509.
[10]FANG Q,BLACK IV W C,BLOCKER H D,et al.A phylogeny of New World Deltocephalus-like leafhopper genera based on mitochondrial 16S ribosomal DNA sequences[J].Mol Phylogenet Evol,1993,2(2):119-131.
[11]TAKIYA D M,TRAN P L,DIETRICH C H,et al.Co‐cladogenesis spanning three phyla:leafhoppers(Insecta:Hemiptera:Cicadellidae)and their dual bacterial symbionts[J].Molecular Ecology,2006,15(13):4175-4191.
[12]DIETRICH C H,FITZGERALD S J,HOLMES J L,et al.Reassessment of Dalbulus leafhopper(Homoptera:Cicadellidae)phylogeny based on mitochondrial DNA sequences[J].Ann Entomol Soc Am,1998,91(5):590-597.
[13]DIETRICH C H,DMITRIEV D A,RAKITOV R A,et al.Phylogeny of Cicadellidae(Cicadomorpha:Membracoidea)based on combined morphological and 28S r DNA sequence data[C]//California:12th International Auchenorrhyncha Congress,Berkeley,CA.2005:7-12.
[14]SIMON C,BUCKLEY T R,FRATI F,et al.Incorporating molecular evolution into phylogenetic analysis,and a new compilation of conserved polymerase chain reaction primers for animal mitochondrial DNA[J].Annu Rev Ecol Evol Syst,2006,37:545-579.
[15]BURLAND T G.DNASTAR’s Lasergene sequence analysis software[M]//MISENER S,KRAWETZ S A.Bioinformatics methods and protocols.Totowa:Humana Press,1999:71-91.
[16]LOWE T M,EDDY S R.t RNAscan-SE:a program for improved detection of transfer RNA genes in genomic sequence[J].Nucleic Acids Res,1997,25(5):955-964.
[17]TAMURA K,PETERSON D,PETERSON N,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Mol Biol Evol,2011,28(10):2731-2739.
[18]PERNA N T,KOCHER T D.Unequal base frequencies and the estimation of substitution rates[J].Mol Biol Evol,1995,12(2):359-361.
[19]WANG H L,YANG J,BOYKIN L M,et al.The characteristics and expression profiles of the mitochondrial genome for the Mediterranean species of the Bemisia tabaci complex[J].BMC Genomics,2013,14(1):401-415.
[20]HUELSENBECK J P,RONQUIST F.MRBAYES:Bayesian inference of phylogenetic trees[J].Bioinformatics,2001,17(8):754-755.
[21]GUINDON S,GASCUEL O.A simple,fast,and accurate algorithm to estimate large phylogenies by maximum likelihood[J].Systematic Biol,2003,52(5):696-704.
[22]WU Y,DAI R,ZHAN H,et al.Complete mitochondrial genome of Drabescoides nuchalis(Hemiptera:Cicadellidae)[J].Mitochondrial DNA A,2016,27(5):3626-3627.
[23]YU P,WANG M,CUI L,et al.The complete mitochondrial genome of Tambocerus sp.(Hemiptera:Cicadellidae)[J].Mitochondr DNA,2017,28(1):133-134.
[24]SONG N,LIANG A P.The complete mitochondrial genome sequence of Geisha distinctissima(Hemiptera:Flatidae)and comparison with other hemipteran insects[J].Acta Biochim Biophys Sin,2009,41(3):206-216.
[25]ZHOU N,WANG M,CUI L,et al.Complete mitochondrial genome of Empoasca vitis(Hemiptera:Cicadellidae)[J].Mitochondrial DNA Part A,2016,27(2):1052-1053.
[26]LIANG A P,GAO J,ZHAO X.Characterization of the complete mitochondrial genome of the treehopper Darthula hardwickii(Hemiptera:Aetalionidae)[J].Mitochondrial DNA Part A,2016,27(5):3291-3292.
[27]WU Y,DAI R,ZHAN H,et al.Complete mitochondrial genome of Drabescoides nuchalis(Hemiptera:Cicadellidae)[J].Mitochondrial DNA A,2016,27(5):3626-3627.
[28]ZHAO X,LIANG A P.Complete DNA sequence of the mitochondrial genome of the treehopper Leptobelus gazella(Membracoidea:Hemiptera)[J].Mitochondrial DNA Part A,2016,27(5):3318-3319.
[29]HUNNICUTT L E,HUNTER W B,MOZORUK J J.The complete mitochondrial genome of the blue-green sharpshooter,Graphocephala atropunctata(Hemiptera:Cicadellidae)[C]//Entomological Society of America Meeting.Reno:2008.
[30]YANG H,LIU J,LIANG A.The complete mitochondrial genome of Cosmoscarata bispecularis(Hemiptera,Cicadomorpha,Cercopoidea,Cercopidae)[J].Mitochondrial DNA Part A,2016,27(6):3957-3958.
[31]牛芳芳,陈静,姜立云,等.两种毛蚜线粒体基因组比较分析[J].应用昆虫学报,2016,53(6):1288-1300.