197份小偃麦渗入系抗条锈病评价与分子鉴定
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摘要
由小麦条锈菌(Puccinia striiformis f. sp. Tritici, Pst)引起的小麦条锈病是世界范围内小麦主要病害之一,培育和种植抗病品种是控制该病害的最有效措施。本研究利用流行条锈菌小种CYR32、CYR33和CYR34对育成的197份小偃麦高代渗入系在成都进行成株期抗条锈病鉴定,并利用Yr50和Yr69的连锁标记进行分子检测,挖掘可能含有新抗病位点的材料,从而为小麦抗病育种拓宽抗源。结果表明:197份小偃麦高代渗入系中,筛选出156个能抗条锈菌小种的株系;结合标记检测结果,筛选到34份含Yr50特征带型的材料,75份含Yr69特征带型的材料,22份材料同时含有Yr50和Yr69的特征带型,其余材料未检测到上述抗条锈病基因。推测这些抗性株系可能具有新的抗条锈病基因。该研究结果将为小麦抗条锈病品种的选育提供新抗源并为发掘新的抗条锈病基因提供依据。
Stripe rust, caused by Puccinia striiformis f. sp. tritici(Pst), is one of the main diseases of wheat worldwide, and breeding and planting resistant varieties is the most effective measure to control the disease. To discover materials that might with new disease resistant sites and broaden wheat disease resistance and cultivation, 197 cultivated wheat-Thinopyrum introgression lines were tested for stripe rust resistance at adult plant stage in Chengdu,based on the prevalent stripe rust species of CYR32, CYR33 and CYR34. Besides, molecular identification was applied for the 197 lines with the linkage markers of two wheat stripe rust resistance genes Yr50 and Yr69. The results indicated that among 197 wheat-Thinopyrum introgression lines, 156 lines exhibited a level of resistance to stripe rust. Combined with the marker identification results, 34 lines containing Yr50 characteristics bands, 75 lines containing Yr69 characteristics bands, 22 lines containing both Yr50 and Yr69 characteristics bands were screened,and the other lines were not detected above stripe rust resistance genes, which suggested that they might possess novel resistance genes. The results of this study would provide a new source for breeding the resistant varieties and lay the foundation for the discovery of new stripe rust resistance genes.
Stripe rust, caused by Puccinia striiformis f. sp. tritici(Pst), is one of the main diseases of wheat worldwide, and breeding and planting resistant varieties is the most effective measure to control the disease. To discover materials that might with new disease resistant sites and broaden wheat disease resistance and cultivation, 197 cultivated wheat-Thinopyrum introgression lines were tested for stripe rust resistance at adult plant stage in Chengdu,based on the prevalent stripe rust species of CYR32, CYR33 and CYR34. Besides, molecular identification was applied for the 197 lines with the linkage markers of two wheat stripe rust resistance genes Yr50 and Yr69. The results indicated that among 197 wheat-Thinopyrum introgression lines, 156 lines exhibited a level of resistance to stripe rust. Combined with the marker identification results, 34 lines containing Yr50 characteristics bands, 75 lines containing Yr69 characteristics bands, 22 lines containing both Yr50 and Yr69 characteristics bands were screened,and the other lines were not detected above stripe rust resistance genes, which suggested that they might possess novel resistance genes. The results of this study would provide a new source for breeding the resistant varieties and lay the foundation for the discovery of new stripe rust resistance genes.
引文
An D.G.,Xu H.X.,and Xu Y.F.,2011,Enhancement of wheat distant hybridization germp lasm,Zhongguo Shengtai Nongye Xuebao(Chinese Journal of Eco-Agriculture),19(5):1011-1019(安调过,许红星,许云峰,2011,小麦远缘杂交种质资源创新,中国生态农业学报,19(5):1011-1019)
Han D.J.,Wang Q.L.,Zhang L.,Wei G.R.,Zeng Q.D.,Zhao J.,Wang X.J.,Huang L.L.,and Kang Z.S.,2010,Evaluation of resistance of current wheat cultivars to stripe rust in Northwest China,North China and the middle and lower reaches of Changjiang River epidemic area,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),43(14):2889-2896(韩德俊,王琪琳,张立,魏国荣,曾庆东,赵杰,王晓杰,黄丽丽,康振生,2010,西北-华北-长江中下游条锈病流行区系当前小麦品种(系)抗条锈病性评价,中国农业科学,43(14):2889-2896)
Hillambroz K.L.,Brownguedira G.L.,and Fellers J.P.,2002,Modified rapid DNA extraction protocol for high throughput microsatellite analysis in wheat,Crop Sci.,42(6):2088-2091
Hou L.Y.,Jia J.Q.,Zhang X.J.,Li X.,Yang Z.J.,Ma J.,Guo H.J.,Zhan H.X.,Qiao L.Y.,and Chang Z.J.,2016,Molecular mapping of the stripe rust resistance gene Yr69 on wheat chromosome 2AS,Plant Disease,100(8):1717-1724
Kang Z.S.,Wang X.J.,Zhao J.,Tang C.L.,and Huang L.L.,2015,Advances in research of pathogenicity and virulence variation of the wheat stripe rust fungus Puccinia striiformis f.sp.Tritici,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),48(17):3439-3453(康振生,王晓杰,赵杰,汤春蕾,黄丽丽,2015,小麦条锈菌致病性及其变异研究进展,中国农业科学,48(17):3439-3453)
Kang Z.S.,Zhao J.,Han D.J.,Zhang H.C.,Wang X.J.,Wang C.F.,Han Q.M.,Guo J.,and Huang L.L.,2010,Status of wheat rust research and control in China,BGRI technical workshop oral presentations,30-31,March,St Petersburg,Russia,pp.50-69
Li G.Q.,Li Z.F.,Yang W.Y.,Zhang Y.,He Z.H.,Xu S.C.,Singh R.P.,Qu Y.Y.,and Xia X.C.,2006,Molecular mapping of stripe rust resistance gene Yr CH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26,Theor.Appl.Genet.,112(8):1434-1440
Liu J.,Chang Z.J.,Zhang X.J.,Yang Z.J.,Li X.,Jia J.Q.,Zhan H.X.,Guo H.J.,and Wang J.M.,2013,Putative Thinopyrum intermedium-derived stripe rust resistance gene Yr50 maps on wheat chromosome arm 4BL,Theor.Appl.Genet.,126(1):265-274
Liu T.G.,Peng Y.L.,Chen W.Q.,and Zhang Z.Y.,2010,First detection of virulence in Puccinia striiformis f.sp.tritici in China to resistance genes Yr24(=Yr26)present in wheat cultivar Chuanmai 42,Plant Disease,94(9):1163
Lowe I.,Jankuloski L.,Chao S.,Chen X.M.,See D.,and Dubcovsky J.,2011,Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploidy wheat,Theor.Appl.Genet.,123(1):143-157
Tester M.,and Langridge P.,2010,Breeding technologies to increase crop production in a changing world,Science,327(5967):818-822
Wan A.M.,Chen X.M.,and He Z.H.,2007,Wheat stripe rust in China,Australian Journal of Agricultural Research,58(6):605-619
Wei G.R.,Han D.J.,Zhao J.,Wang X.J.,Wang Q.L.,Huang L.L.,and Kang Z.S.,2011,Identification and evaluation of adult plant resistance to stripe rust in wheat germplasms,Mailei Zuowu Xuebao(Journal of Triticeae Crops),31(2):376-381(魏国荣,韩德俊,赵杰,王晓杰,王琪琳,黄丽丽,康振生,2011,小麦成株期抗条锈病种质筛选与评价,麦类作物学报,31(2):376-381)
Wellings C.R.,2011,Global status of stripe rust:a review of historical and current threats,Euphytica,179(1):129-141
Zeng S.M.,and Luo Y.,2006,Long-distance spread and interregional epidemics of wheat stripe rust in China,Plant Disease,90(8):980-988
Zhang P.,Mcintosh R.A.,Hoxha S.,and Dong C.,2009,Wheat stripe rust resistance genes Yr5 and Yr7 are allelic,Theor.Appl.Genet.,120(1):25-29
Zhang Q.Q.,1999,The methodology of multi-resistance to diseases in wheat distant breeding,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),12(1):32-37(张庆勤,1999,小麦远缘杂交中兼抗育种方法研究,西南农业学报,12(1):32-37)
Zhang Q.Q.,2000,Breeding,identification and use of wheat simultaneous resistant sources,Guizhou Kexue(Guizhou Science),18(1-2):108-111(张庆勤,2000,小麦兼抗性抗源的选育、鉴定和利用,贵州科学,18(1-2):108-111)
Zhang X.J.,Han D.J.,Zeng Q.D.,Duan Y.H.,Yuan F.P.,Shi J.D.,Wang Q.L.,Wu J.H.,Huang L.L.,and Kang Z.S.,2013,Fine mapping of wheat stripe rust resistance gene Yr26 based on collinearity of wheat with Brachypodium distachyon and rice,PLoS One,8(3):e57885
Zheng D.S.,2005,Use of cereal crop wild relatives in crop breeding in China,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),6(3):354-358(郑殿升,2005,中国禾谷类作物野生近缘植物在育种中的利用,植物遗传资源学报,6(3):354-358)
Zhou X.L.,Han D.J.,Chen X.M.,Gou H.L.,Guo S.J.,Rong L.,Wang Q.L.,Huang L.L.,and Kang Z.S.,2014,Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34,Theor.Appl.Genet.,127(11):2349-2358
Han D.J.,Wang Q.L.,Zhang L.,Wei G.R.,Zeng Q.D.,Zhao J.,Wang X.J.,Huang L.L.,and Kang Z.S.,2010,Evaluation of resistance of current wheat cultivars to stripe rust in Northwest China,North China and the middle and lower reaches of Changjiang River epidemic area,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),43(14):2889-2896(韩德俊,王琪琳,张立,魏国荣,曾庆东,赵杰,王晓杰,黄丽丽,康振生,2010,西北-华北-长江中下游条锈病流行区系当前小麦品种(系)抗条锈病性评价,中国农业科学,43(14):2889-2896)
Hillambroz K.L.,Brownguedira G.L.,and Fellers J.P.,2002,Modified rapid DNA extraction protocol for high throughput microsatellite analysis in wheat,Crop Sci.,42(6):2088-2091
Hou L.Y.,Jia J.Q.,Zhang X.J.,Li X.,Yang Z.J.,Ma J.,Guo H.J.,Zhan H.X.,Qiao L.Y.,and Chang Z.J.,2016,Molecular mapping of the stripe rust resistance gene Yr69 on wheat chromosome 2AS,Plant Disease,100(8):1717-1724
Kang Z.S.,Wang X.J.,Zhao J.,Tang C.L.,and Huang L.L.,2015,Advances in research of pathogenicity and virulence variation of the wheat stripe rust fungus Puccinia striiformis f.sp.Tritici,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),48(17):3439-3453(康振生,王晓杰,赵杰,汤春蕾,黄丽丽,2015,小麦条锈菌致病性及其变异研究进展,中国农业科学,48(17):3439-3453)
Kang Z.S.,Zhao J.,Han D.J.,Zhang H.C.,Wang X.J.,Wang C.F.,Han Q.M.,Guo J.,and Huang L.L.,2010,Status of wheat rust research and control in China,BGRI technical workshop oral presentations,30-31,March,St Petersburg,Russia,pp.50-69
Li G.Q.,Li Z.F.,Yang W.Y.,Zhang Y.,He Z.H.,Xu S.C.,Singh R.P.,Qu Y.Y.,and Xia X.C.,2006,Molecular mapping of stripe rust resistance gene Yr CH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26,Theor.Appl.Genet.,112(8):1434-1440
Liu J.,Chang Z.J.,Zhang X.J.,Yang Z.J.,Li X.,Jia J.Q.,Zhan H.X.,Guo H.J.,and Wang J.M.,2013,Putative Thinopyrum intermedium-derived stripe rust resistance gene Yr50 maps on wheat chromosome arm 4BL,Theor.Appl.Genet.,126(1):265-274
Liu T.G.,Peng Y.L.,Chen W.Q.,and Zhang Z.Y.,2010,First detection of virulence in Puccinia striiformis f.sp.tritici in China to resistance genes Yr24(=Yr26)present in wheat cultivar Chuanmai 42,Plant Disease,94(9):1163
Lowe I.,Jankuloski L.,Chao S.,Chen X.M.,See D.,and Dubcovsky J.,2011,Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploidy wheat,Theor.Appl.Genet.,123(1):143-157
Tester M.,and Langridge P.,2010,Breeding technologies to increase crop production in a changing world,Science,327(5967):818-822
Wan A.M.,Chen X.M.,and He Z.H.,2007,Wheat stripe rust in China,Australian Journal of Agricultural Research,58(6):605-619
Wei G.R.,Han D.J.,Zhao J.,Wang X.J.,Wang Q.L.,Huang L.L.,and Kang Z.S.,2011,Identification and evaluation of adult plant resistance to stripe rust in wheat germplasms,Mailei Zuowu Xuebao(Journal of Triticeae Crops),31(2):376-381(魏国荣,韩德俊,赵杰,王晓杰,王琪琳,黄丽丽,康振生,2011,小麦成株期抗条锈病种质筛选与评价,麦类作物学报,31(2):376-381)
Wellings C.R.,2011,Global status of stripe rust:a review of historical and current threats,Euphytica,179(1):129-141
Zeng S.M.,and Luo Y.,2006,Long-distance spread and interregional epidemics of wheat stripe rust in China,Plant Disease,90(8):980-988
Zhang P.,Mcintosh R.A.,Hoxha S.,and Dong C.,2009,Wheat stripe rust resistance genes Yr5 and Yr7 are allelic,Theor.Appl.Genet.,120(1):25-29
Zhang Q.Q.,1999,The methodology of multi-resistance to diseases in wheat distant breeding,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),12(1):32-37(张庆勤,1999,小麦远缘杂交中兼抗育种方法研究,西南农业学报,12(1):32-37)
Zhang Q.Q.,2000,Breeding,identification and use of wheat simultaneous resistant sources,Guizhou Kexue(Guizhou Science),18(1-2):108-111(张庆勤,2000,小麦兼抗性抗源的选育、鉴定和利用,贵州科学,18(1-2):108-111)
Zhang X.J.,Han D.J.,Zeng Q.D.,Duan Y.H.,Yuan F.P.,Shi J.D.,Wang Q.L.,Wu J.H.,Huang L.L.,and Kang Z.S.,2013,Fine mapping of wheat stripe rust resistance gene Yr26 based on collinearity of wheat with Brachypodium distachyon and rice,PLoS One,8(3):e57885
Zheng D.S.,2005,Use of cereal crop wild relatives in crop breeding in China,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),6(3):354-358(郑殿升,2005,中国禾谷类作物野生近缘植物在育种中的利用,植物遗传资源学报,6(3):354-358)
Zhou X.L.,Han D.J.,Chen X.M.,Gou H.L.,Guo S.J.,Rong L.,Wang Q.L.,Huang L.L.,and Kang Z.S.,2014,Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34,Theor.Appl.Genet.,127(11):2349-2358