基于近红外光谱技术的白酒基酒中乳酸的快速检测
|
摘要
目的:研究现代近红外光谱技术快速检测白酒基酒中的乳酸含量。方法:采用高效液相色谱法测定基酒样品中乳酸的化学值,与近红外透射光谱相结合,建立白酒基酒中乳酸的定量检测模型。结果:最佳预处理方法为一阶导数+多元散射校正(MSC),最优波段谱为6102~5450 cm~(-1)。对模型进行优化和检验,得到校正集样品的化学值与预测值的决定系数(R2)为0.9771,校正标准偏差(RMSECV)为0.1825;验证集的决定系数(R2)为0.9808,预测标准偏差(RMSEP)为0.1475。结论:近红外光谱法快速有效,所建模型具有很好的预测效果,模型的精密度和稳定性良好,为白酒生产中乳酸含量的检测提供方法指导。
Objective: To study the rapid determination of lactic acid content in base liquor by Modern Near Infrared Spectroscopy.Methods: High performance liquid chromatography was used to determine the chemical value of lactic acid in base liquor,which was combined with near infrared transmission spectrum,and established the quantitative detection model of lactic acid in base liquor. Results: The best spectral pretreatment method was the first derivative + multivariate scatter correction( MSC),the optimal band spectrum was 6102 ~ 5450 cm-1. Through optimized and validated the model,the determination coefficient( R2) between the chemical value and the near infrared predicted value was 0.9771; the correction standard deviation( RMSECV) was 0.1825; the determinant coefficient of the validation set( R~2) was 0.9808,the prediction standard deviation( RMSEP) was 0.1475.Conclusion: Near infrared detection method was fast and effective,the model had good prediction effect,and the precision and stability of the model was good,providing guidance for the determination of lactic acid content in liquor industry.
Objective: To study the rapid determination of lactic acid content in base liquor by Modern Near Infrared Spectroscopy.Methods: High performance liquid chromatography was used to determine the chemical value of lactic acid in base liquor,which was combined with near infrared transmission spectrum,and established the quantitative detection model of lactic acid in base liquor. Results: The best spectral pretreatment method was the first derivative + multivariate scatter correction( MSC),the optimal band spectrum was 6102 ~ 5450 cm-1. Through optimized and validated the model,the determination coefficient( R2) between the chemical value and the near infrared predicted value was 0.9771; the correction standard deviation( RMSECV) was 0.1825; the determinant coefficient of the validation set( R~2) was 0.9808,the prediction standard deviation( RMSEP) was 0.1475.Conclusion: Near infrared detection method was fast and effective,the model had good prediction effect,and the precision and stability of the model was good,providing guidance for the determination of lactic acid content in liquor industry.
引文
[1]张建林,穆文斌,李加运,等.利用HPLC测定白酒中乳酸的研究[J].酿酒,2003,30(2):14-15.
[2]李维青.浓香型白酒与乳酸菌、乳酸、乳酸乙酯[J].酿酒,2010,37(3):90-93.
[3]余剑霞.气相色谱法测定白酒中的乳酸[J].酿酒科技,2002(3):69-70.
[4]冯向东.高效液相色谱法测定白酒中乳酸和乙酸含量[J].酿酒科技,2009(5):115-116.
[5]李斌,王颖.高效液相色谱法测定浓香型白酒中乳酸的含量[J].现代农业科技,2016(4):291-292.
[6]郭福阳,刘辉,从志会,等.高效液相色谱法检测白酒基酒中的乳酸[J].当代化工,2015,44(12):2923-2925.
[7]李志斌,姚珍.白酒中乳酸的测定方法[J].酿酒科技,2003(5):81-82.
[8]马雷,沈小梅,李安军,等.超高效液相色谱法测定白酒中乳酸的含量[J].食品与发酵科技,2016,52(1):84-86.
[9]徐广通,袁洪福,陆婉珍.现代近红外光谱技术及应用进展[J].光谱学与光谱分析,2000,20(2):134-142.
[10]欧阳爱国,刘军,王亚平.乙醇汽油含量的近红外光谱检测研究[J].激光与红外,2012,42(8):901-904.
[11]Yu H Y,Niu X Y,Lin H J,et al.A feasibility study on online determination of rice wine composition by Vis-NIR spectroscopy and least-squares support vector machines[J].Food Chemistry,2009,113(1):291-296.
[12]Yao J,Yang Q,Sun C Y,et al.Qualitatlve analysis of methanol in the forged wine using infrared spectrophotometer[J].Chinese Journal of Spectroscopy Labortory,2000,17(1):35-37.
[13]王毅,马翔,温亚东,等.应用近红外光谱分析不同年度工业分级烟叶的特性[J].光谱学与光谱分析,2012,32(11):3014-3018.
[14]李万江,冯海青.近红外光谱法对藿香正气水中乙醇的定量分析[J].医药导报,2011,30(10):1351-1352.
[15]李其禄,王斐,王金凤,等.不同近红外光谱仪用于酒石酸美托洛尔片厂家鉴别的对比研究[J].光谱学与光谱分析,2015(10):2924-2929.
[16]文韬,郑立章,龚中良,等.基于近红外光谱技术的茶油原产地快速鉴别[J].农业工程学报,2016,32(16):293-299.
[17]刘晓晔,汤晓艳,孙宝忠,等.两种近红外光谱分辨率预测牛肉营养成分的比较研究[J].食品工业科技,2013,34(3):302-305.
[18]吴同,谭超.近红外光谱同时测定白酒中总酯和杂醇油[J].化学研究与应用,2016,28(10):1460-1463.
[19]胡小邦,吕进,刘辉军,等.基于近红外透射光谱的黄酒酒精度、酸度检测模型研究[J].光电工程,2010,37(10):122-127.
[20]王海英,杨玉珍,任国军,等.利用近红外技术对河套原酒入库指标的检测研究[J].酿酒科技,2017(1):37-41.
[21]徐玮,谭红,包娜,等.近红外光谱法快速测定白酒中的酒精度[J].中国农学通报,2010,26(19):70-72.
[22]先春,陈仁远,王俊,等.近红外光谱结合聚类分析对不同风格酱香型白酒的研究[J].酿酒科技,2016(3):49-51,56.
[23]刘建学,张卫卫,韩四海,等.白酒基酒中己酸、乙酸的近红外快速检测[J].食品科学,2016,37(4):181-185.
[24]张卫卫,刘建学,韩四海,等.白酒基酒中醛类物质的傅里叶变换近红外光谱检测[J].食品科学,2016,37(6):111-115.
[25]孙宗保,辛新,揍小波,等.傅里叶变换红外光谱结合化学计量学方法对白酒基酒的快速定性和定量分析[J].光谱学与光谱分析,2017,37(9):2756-2762.
[26]石鲁珍,张景川,王彦群,等,马氏距离与浓度残差剔除近红外异常样品研究.中国农机化学报,2016(6):99-103.
[2]李维青.浓香型白酒与乳酸菌、乳酸、乳酸乙酯[J].酿酒,2010,37(3):90-93.
[3]余剑霞.气相色谱法测定白酒中的乳酸[J].酿酒科技,2002(3):69-70.
[4]冯向东.高效液相色谱法测定白酒中乳酸和乙酸含量[J].酿酒科技,2009(5):115-116.
[5]李斌,王颖.高效液相色谱法测定浓香型白酒中乳酸的含量[J].现代农业科技,2016(4):291-292.
[6]郭福阳,刘辉,从志会,等.高效液相色谱法检测白酒基酒中的乳酸[J].当代化工,2015,44(12):2923-2925.
[7]李志斌,姚珍.白酒中乳酸的测定方法[J].酿酒科技,2003(5):81-82.
[8]马雷,沈小梅,李安军,等.超高效液相色谱法测定白酒中乳酸的含量[J].食品与发酵科技,2016,52(1):84-86.
[9]徐广通,袁洪福,陆婉珍.现代近红外光谱技术及应用进展[J].光谱学与光谱分析,2000,20(2):134-142.
[10]欧阳爱国,刘军,王亚平.乙醇汽油含量的近红外光谱检测研究[J].激光与红外,2012,42(8):901-904.
[11]Yu H Y,Niu X Y,Lin H J,et al.A feasibility study on online determination of rice wine composition by Vis-NIR spectroscopy and least-squares support vector machines[J].Food Chemistry,2009,113(1):291-296.
[12]Yao J,Yang Q,Sun C Y,et al.Qualitatlve analysis of methanol in the forged wine using infrared spectrophotometer[J].Chinese Journal of Spectroscopy Labortory,2000,17(1):35-37.
[13]王毅,马翔,温亚东,等.应用近红外光谱分析不同年度工业分级烟叶的特性[J].光谱学与光谱分析,2012,32(11):3014-3018.
[14]李万江,冯海青.近红外光谱法对藿香正气水中乙醇的定量分析[J].医药导报,2011,30(10):1351-1352.
[15]李其禄,王斐,王金凤,等.不同近红外光谱仪用于酒石酸美托洛尔片厂家鉴别的对比研究[J].光谱学与光谱分析,2015(10):2924-2929.
[16]文韬,郑立章,龚中良,等.基于近红外光谱技术的茶油原产地快速鉴别[J].农业工程学报,2016,32(16):293-299.
[17]刘晓晔,汤晓艳,孙宝忠,等.两种近红外光谱分辨率预测牛肉营养成分的比较研究[J].食品工业科技,2013,34(3):302-305.
[18]吴同,谭超.近红外光谱同时测定白酒中总酯和杂醇油[J].化学研究与应用,2016,28(10):1460-1463.
[19]胡小邦,吕进,刘辉军,等.基于近红外透射光谱的黄酒酒精度、酸度检测模型研究[J].光电工程,2010,37(10):122-127.
[20]王海英,杨玉珍,任国军,等.利用近红外技术对河套原酒入库指标的检测研究[J].酿酒科技,2017(1):37-41.
[21]徐玮,谭红,包娜,等.近红外光谱法快速测定白酒中的酒精度[J].中国农学通报,2010,26(19):70-72.
[22]先春,陈仁远,王俊,等.近红外光谱结合聚类分析对不同风格酱香型白酒的研究[J].酿酒科技,2016(3):49-51,56.
[23]刘建学,张卫卫,韩四海,等.白酒基酒中己酸、乙酸的近红外快速检测[J].食品科学,2016,37(4):181-185.
[24]张卫卫,刘建学,韩四海,等.白酒基酒中醛类物质的傅里叶变换近红外光谱检测[J].食品科学,2016,37(6):111-115.
[25]孙宗保,辛新,揍小波,等.傅里叶变换红外光谱结合化学计量学方法对白酒基酒的快速定性和定量分析[J].光谱学与光谱分析,2017,37(9):2756-2762.
[26]石鲁珍,张景川,王彦群,等,马氏距离与浓度残差剔除近红外异常样品研究.中国农机化学报,2016(6):99-103.