菌糠强化微生物降解石油污染土壤修复研究
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摘要
采用菌糠协同高效石油烃降解菌Microbacterium.sp.Q2进行石油污染土壤修复试验研究,分别设置菌糠固定化微生物组(SIM)、菌糠-游离菌组(SMSB)、菌糠单独组(SMS)和对照组(CK)4组修复实验.考察不同处理方式下对石油污染土壤微生物数量、酶活性和石油烃降解效果的差异性并确定石油污染土壤的最佳修复方案.结果表明:不同修复方式下,SIM组的土壤呼吸强度、微生物数量及酶活性较其他组有明显提高,其对石油烃去除率分别比其他3组提高11.84%、22.15%、54.09%.土壤中脱氢酶活性以及微生物活性与石油烃降解率的相关性显著,此外菌糠固定化微生物对石油污染土壤修复具有生物强化和生物刺激协同的作用机制.
The spent mushroom substrate(SS) and Microbacterium.sp.Q2 bacteria were used to remediate petroleum-contaminated soil and four different treatments which contained spent mushroom substrate immobilized microorganisms(SIM), spent mushroom substrate-free bacteria(SMSB), spent mushroom substrate alone(SMS) and no treatment(CK) were designed in this paper. The aim of this study is to investigate soil microbial number, enzyme activities and the degradation rate of total petroleum hydrocarbon(TPH),exploring an ideal reparative treatment. The results indicated that soil respiration intensity, microbial number and enzyme activities of SIM group were obviously higher than others and the removal rate of TPH was highest which was increased by 11.84%, 22.15% and 54.09% compared with other treatments. Furthermore it is showed that the dehydrogenase activity and microbial activity were significantly correlated with the degradation rate of TPH and immobilized microorganism had the synergistic mechanism of bioaugmentation and biostimulation for remediation of petroleum contaminated soil.
The spent mushroom substrate(SS) and Microbacterium.sp.Q2 bacteria were used to remediate petroleum-contaminated soil and four different treatments which contained spent mushroom substrate immobilized microorganisms(SIM), spent mushroom substrate-free bacteria(SMSB), spent mushroom substrate alone(SMS) and no treatment(CK) were designed in this paper. The aim of this study is to investigate soil microbial number, enzyme activities and the degradation rate of total petroleum hydrocarbon(TPH),exploring an ideal reparative treatment. The results indicated that soil respiration intensity, microbial number and enzyme activities of SIM group were obviously higher than others and the removal rate of TPH was highest which was increased by 11.84%, 22.15% and 54.09% compared with other treatments. Furthermore it is showed that the dehydrogenase activity and microbial activity were significantly correlated with the degradation rate of TPH and immobilized microorganism had the synergistic mechanism of bioaugmentation and biostimulation for remediation of petroleum contaminated soil.
引文
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[10]宁大亮,王慧,王立华,等.难降解有机物对白腐真菌P450的诱导及P450的作用[J].中国环境科学,2009,29(4):407-412.Ning D L,Wang H,Wang L H,et al.Induction and function of cytochrome P450 in degradation of refractory organic chemicals by Phanerochaete chrysosporium[J].China Environment Science,2009,29(4):407-412.
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[13]Ebadi A,Khoshkholgh Sima N A,Olamaee M,et al.Remediation of saline soils contaminated with crude oil using the halophyte Salicornia persica in conjunction with hydrocarbon-degrading bacteria[J].Journal of Environmental Management,2018,219:260-268.
[14]王聪颖,王芳,王涛,等.生物强化和生物刺激对土壤中PAHs降解的影响[J].中国环境科学,2010,30(1):121-127.Wang T,Yin C Q,Bian Y R,et al.Effects of bioaugmentation and biostimulation Oil PAHs degradation in soil[J].China Environment Science.2010,30(1):121-127.
[15]贾建丽,李广贺,张旭.石油污染土壤生物修复的中试系统构建与运行效果[J].中国环境科学,2005,25(3):339-342.Jia J L,Li G H,Zhang X.Design establishment and operation effect of the pilot system in the oil contaminated soils bioremediation[J].China Environment Science,2005,25(3):339-342.
[16]唐玉姝,魏朝富,颜廷梅,等.土壤质量生物学指标研究进展[J].土壤,2007,39(2):157-163.Tang Y S,Wei C F,Yan T M,et al.Biological indicator of soil quality:A review[J].Soils.2007,39(2):157-163.
[17]杨茜,吴蔓莉,聂麦茜,等.石油污染土壤的生物修复技术及微生物生态效应[J].环境科学,2015,36(5):1856-1863.Yang Q,Wu M L,Nie M Q,et al.Effects and biological response on bioremediation of petroleum contaminated soil[J].Environmental Science,2015,36(5):1856-1863.
[18]姜睿玲,杨统一,唐玉斌,等.多环芳烃污染对桑园土壤微生物结构及种群多样性的影响[J].中国环境科学,2012,32(9):1655-1661.Jiang R L,Yang T Y,Tang Y B,et al.Effect of PAHs pollution on micmbial stucture and population diversity of mulberry orchard soil[J].China Environment Science,2012,32(9):1655-1661.
[19]Li X,Wu Y,Lin X,et al.Dissipation of polycyclic aromatic hydrocarbons(PAHs)in soil microcosms amended with mushroom cultivation substrate[J].Soil Biology and Biochemistry,2012,47:191-197.
[20]Karanasios E,Tsiropoulos N G,Karpouzas D G,et al.Novel biomixtures based on local Mediterranean lignocellulosic materials:Evaluation for use in biobed systems[J].Chemosphere,2010,80(8):914-921.
[21]韩慧龙,汤晶,江皓,等.真菌-细菌修复石油污染土壤的协同作用机制研究[J].环境科学,2008,29(1):189-195.Han H L,Tang J,Jiang H,et al.Synergy between fungi and bacteria in fungi-bacteria augmented remediation of petroleum-contaminated soil[J].Environment Science,2008,29(1):189-195.
[22]肖敏,高彦征,凌婉婷,等.菲、芘污染土壤中丛枝菌根真菌对土壤酶活性的影响[J].中国环境科学,2009,29(6):668-672.Xiao M,Gao Y Z,Ling W T,et al.Effects of arbuscular mycorrhizal fungi on enzymes activity in soils contaminated by phenanthrene and pyrene[J].China Environmental Science,2009,29(6):668-672.
[23]曾宪成,韩立新,张彩凤,等.我国腐植酸类表面活性剂应用概述[J].腐植酸,2011,(6):1-4.Zeng X C,Han L X,Zhang C F,et al.Outline on development and application of Chinese humic acid surfactants[J].Humic Acid,2011,(6):1-4.
[24]Villalobos M,Avila-Forcada A P,Gutierrez-Ruiz M E.An improved gravimetric method to determine total petroleum hydrocarbons in contaminated soils[J].Water,Air,and Soil Pollution,2008,194(1-4):151-161.
[25]Wang S J,Wang X.Bioremediation of petroleum contaminated soils collected all around China:The extensive application and the microbial mechanism[J].Petroleum Science and Technology,2018,36(13):974-980.
[26]Du J,Sun P,Feng Z,et al.The biosorption capacity of biochar for4-bromodiphengl ether:study of its kinetics,mechanism,and use as a carrier for immobilized bacteria[J].Environmental Science and Pollution Research,2016,23(4):3770-3780.
[27]郭婷,张承东,张清敏.生物修复石油污染盐碱土壤小试模拟系统中土壤性质与微生物特性变化[J].中国环境科学,2010,30(8):1123-1129.Guo T,Zhang C D,Zhang Q M,et al.Changs of soil properties and microorganism characteristic during bioremediation of petroleumcontaminated saline soil in microscal simulation study[J].China Environmental Science,2010,30(8):1123-1129.
[28]李静华.固定化微生物强化修复石油污染土壤的研究[D].广州:华南理工大学,2017.Li J H.Bioaugmentation of crude-oil contaminated soil by immobilized microorganisms[D].Guangzhou:South China University of Technology,2017.
[2]钱林波,元妙新,陈宝梁.固定化微生物技术修复PAHs污染土壤的研究进展[J].环境科学,2012,33(5):1767-1776.Qian L B,Yuan M X,Chen B L.Research progress about bioremediation of polycyclic aromatic hydrocarbons contaminated soil with immobilized microorganism technique[J].Environmental Science,2012,33(5):1767-1776.
[3]Varjani S J,Upasani V N.A new look on factors affecting microbial degradation of petroleum hydrocarbon pollutants[J].International Biodeterioration&Biodegradation,2017,120:71-83.
[4]王丽萍,李丹,许锐伟,等.专性菌系对石油烃污染土壤的修复性能[J].中国环境科学,2018,38(4):1417-1423.Wang L P,Li D,Xu R W,et al.Remediation performance of constructed specific bacteria on petroleum hydrocarbon contaminated soil[J].China Environment Science,2018,38(4):1417-1423.
[5]Mohd Hanafi F H,Rezania S,Mat Taib S,et al.Environmentally sustainable applications of agro-based spent mushroom substrate(SMS):an overview[J].Journal of Material Cycles and Waste Management,2018,20(3):1383-1396.
[6]Wu J,Zhang T,Chen C,et al.Spent substrate of Ganodorma lucidum as a new bio-adsorbent for adsorption of three typical dyes[J].Bioresource Technology,2018,266:134-138.
[7]张秀霞,张守娟,张涵,等.固定化微生物对石油污染土壤生物学特性的影响[J].石油学报(石油加工),2015,31(1):112-118.Zhang X X,Zhang S J,Zhang H,et al.Influence of immobilized microorganism on biological characteristics of petroleumcontaminated soil[J].Acta Petrolei Sinica(Petroleum Processing Section),2015,31(1):112-118.
[8]王玉建,李红玉.固定化微生物在废水处理中的研究及进展[J].生物技术,2006,16(1):94-96.Wang Y J,Li H Y.Advances in immobilized microorganism and it’s Research on Waste Water Treatment[J].Biotechnology,2006,16(1):94-96.
[9]Asemoloye M D,Jonathan S G,Jayeola A A,et al.Mediational influence of spent mushroom compost on phytoremediation of blackoil hydrocarbon polluted soil and response of Megathyrsus maximus Jacq[J].Journal of Environmental Management,2017,200:253-262.
[10]宁大亮,王慧,王立华,等.难降解有机物对白腐真菌P450的诱导及P450的作用[J].中国环境科学,2009,29(4):407-412.Ning D L,Wang H,Wang L H,et al.Induction and function of cytochrome P450 in degradation of refractory organic chemicals by Phanerochaete chrysosporium[J].China Environment Science,2009,29(4):407-412.
[11]滕芝.石油污染土壤的微生物强化修复作用及其机理研究[D].青岛:中国石油大学(华东),2012.Teng Z.Study on bioaugmentation repair of oil-polluted soil and its mechanism[D].Qingdao:China University of Petroleum(East China),2012.
[12]刘海芳,马军辉,金辽,等.水稻土FDA水解酶活性的测定方法及应用[J].土壤学报,2009,46(2):365-367.Liu H F,Ma J H,Jin L,et al.Determination of activity of hydrocarbon in paddy soils application in Taihu Lake region[J].Acta Pedologica Sinica.2009,46(2):365-367.
[13]Ebadi A,Khoshkholgh Sima N A,Olamaee M,et al.Remediation of saline soils contaminated with crude oil using the halophyte Salicornia persica in conjunction with hydrocarbon-degrading bacteria[J].Journal of Environmental Management,2018,219:260-268.
[14]王聪颖,王芳,王涛,等.生物强化和生物刺激对土壤中PAHs降解的影响[J].中国环境科学,2010,30(1):121-127.Wang T,Yin C Q,Bian Y R,et al.Effects of bioaugmentation and biostimulation Oil PAHs degradation in soil[J].China Environment Science.2010,30(1):121-127.
[15]贾建丽,李广贺,张旭.石油污染土壤生物修复的中试系统构建与运行效果[J].中国环境科学,2005,25(3):339-342.Jia J L,Li G H,Zhang X.Design establishment and operation effect of the pilot system in the oil contaminated soils bioremediation[J].China Environment Science,2005,25(3):339-342.
[16]唐玉姝,魏朝富,颜廷梅,等.土壤质量生物学指标研究进展[J].土壤,2007,39(2):157-163.Tang Y S,Wei C F,Yan T M,et al.Biological indicator of soil quality:A review[J].Soils.2007,39(2):157-163.
[17]杨茜,吴蔓莉,聂麦茜,等.石油污染土壤的生物修复技术及微生物生态效应[J].环境科学,2015,36(5):1856-1863.Yang Q,Wu M L,Nie M Q,et al.Effects and biological response on bioremediation of petroleum contaminated soil[J].Environmental Science,2015,36(5):1856-1863.
[18]姜睿玲,杨统一,唐玉斌,等.多环芳烃污染对桑园土壤微生物结构及种群多样性的影响[J].中国环境科学,2012,32(9):1655-1661.Jiang R L,Yang T Y,Tang Y B,et al.Effect of PAHs pollution on micmbial stucture and population diversity of mulberry orchard soil[J].China Environment Science,2012,32(9):1655-1661.
[19]Li X,Wu Y,Lin X,et al.Dissipation of polycyclic aromatic hydrocarbons(PAHs)in soil microcosms amended with mushroom cultivation substrate[J].Soil Biology and Biochemistry,2012,47:191-197.
[20]Karanasios E,Tsiropoulos N G,Karpouzas D G,et al.Novel biomixtures based on local Mediterranean lignocellulosic materials:Evaluation for use in biobed systems[J].Chemosphere,2010,80(8):914-921.
[21]韩慧龙,汤晶,江皓,等.真菌-细菌修复石油污染土壤的协同作用机制研究[J].环境科学,2008,29(1):189-195.Han H L,Tang J,Jiang H,et al.Synergy between fungi and bacteria in fungi-bacteria augmented remediation of petroleum-contaminated soil[J].Environment Science,2008,29(1):189-195.
[22]肖敏,高彦征,凌婉婷,等.菲、芘污染土壤中丛枝菌根真菌对土壤酶活性的影响[J].中国环境科学,2009,29(6):668-672.Xiao M,Gao Y Z,Ling W T,et al.Effects of arbuscular mycorrhizal fungi on enzymes activity in soils contaminated by phenanthrene and pyrene[J].China Environmental Science,2009,29(6):668-672.
[23]曾宪成,韩立新,张彩凤,等.我国腐植酸类表面活性剂应用概述[J].腐植酸,2011,(6):1-4.Zeng X C,Han L X,Zhang C F,et al.Outline on development and application of Chinese humic acid surfactants[J].Humic Acid,2011,(6):1-4.
[24]Villalobos M,Avila-Forcada A P,Gutierrez-Ruiz M E.An improved gravimetric method to determine total petroleum hydrocarbons in contaminated soils[J].Water,Air,and Soil Pollution,2008,194(1-4):151-161.
[25]Wang S J,Wang X.Bioremediation of petroleum contaminated soils collected all around China:The extensive application and the microbial mechanism[J].Petroleum Science and Technology,2018,36(13):974-980.
[26]Du J,Sun P,Feng Z,et al.The biosorption capacity of biochar for4-bromodiphengl ether:study of its kinetics,mechanism,and use as a carrier for immobilized bacteria[J].Environmental Science and Pollution Research,2016,23(4):3770-3780.
[27]郭婷,张承东,张清敏.生物修复石油污染盐碱土壤小试模拟系统中土壤性质与微生物特性变化[J].中国环境科学,2010,30(8):1123-1129.Guo T,Zhang C D,Zhang Q M,et al.Changs of soil properties and microorganism characteristic during bioremediation of petroleumcontaminated saline soil in microscal simulation study[J].China Environmental Science,2010,30(8):1123-1129.
[28]李静华.固定化微生物强化修复石油污染土壤的研究[D].广州:华南理工大学,2017.Li J H.Bioaugmentation of crude-oil contaminated soil by immobilized microorganisms[D].Guangzhou:South China University of Technology,2017.