植物源性食品中氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺残留的LC-MS/MS检测方法研究

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英文篇名：Determination method of fluopicolide,fenaminstrobin and chlorantraniliprole residues in plant foods by LC-MS/MS 作者：司文帅 ; 张颖 ; 张其才 ; 韩奕奕 ; 杨晓君 ; 白冰 ; 宋卫国 英文作者：SI Wen-shuai;ZHANG Ying;ZHANG Qi-cai;HAN Yi-yi;YANG Xiao-jun;BAI Bing;SONG Wei-guo;Institute for Agro-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences;Shanghai University of Medicine & Health Sciences;Agri-products Quality and Safety Testing Center of Shanghai; 关键词：植物源性食品 ; 氟吡菌胺 ; 烯肟菌胺 ; 氯虫苯甲酰胺 ; 液相色谱-串联质谱 英文关键词：Plant foods;;Fluopicolide;;Fenaminstrobin;;Chlorantraniliprole;;Liquid chromatographytandem mass spectrometry(LC-MS/MS) 中文刊名：SHLB 英文刊名：Acta Agriculturae Shanghai 机构：上海市农业科学院农产品质量标准与检测技术研究所;上海健康医学院;上海市农产品质量安全检测中心; 出版日期：2019-01-30 出版单位：上海农业学报 年：2019 期：v.35 基金：上海市农委科技兴农重点攻关项目[沪农科攻字(2015)第4-7号];; 上海市市级农口系统青年人才成长计划[沪农青字(2016)第1-25号];; 上海市科委长三角合作项目(16395810400) 语种：中文; 页：SHLB201901015 页数：4 CN：01 ISSN：31-1405/S 分类号：81-84

摘要

采用液相色谱-串联质谱仪(LC-MS/MS)建立了植物源性食品中氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺残留的定量分析方法,样品经过乙腈涡旋提取后,无水MgSO4、PSA和C18分散固相萃取净化,LC-MS/MS检测。结果表明:在2—100μg/L的线性范围内,氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺的峰面积与其浓度呈良好线性关系,方法检出限为2μg/kg;在水稻、西瓜、青菜、番茄和黄瓜5种基质中,10μg/kg、20μg/kg和40μg/kg三个添加水平下,氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺回收率为88. 2%—107. 6%,相对标准偏差为1. 2%—8. 2%。该方法可以满足植物源性食品中氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺残留检测的需要。
        The liquid chromatography-tandem mass spectrometry( LC-MS/MS) was developed to analyze fluopicolide,fenaminstrobin and chlorantraniliprole residues in plant foods. After extracted from sample with acetonitrile and cleaned up by dispersive solid-phase extraction with PSA and C18,fluopicolide,fenaminstrobin and chlorantraniliprole was determined by LC-MS/MS. As a result,The calibration curves showed good linearity over the concentration range of 2. 0—100 μg/L with correlation eoefficients above 0. 998,and the limits of determination( LOD) was 2. 0 μg/kg. The recoveries of fluopicolide,fenaminstrobin and chlorantraniliprole residues in plant foods were in the range of 88. 2% —107. 6% at three spiked levels( 10 μg/kg,20 μg/kg,40 μg/kg),and the relative standard deviations were 1. 2% —8. 2%.

引文

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