基于多巴胺共振散射光谱法测定牛奶中的三聚氰胺
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摘要
在氢氧化钠介质中,氯金酸与多巴胺发生氧化还原反应生成金纳米粒子而使体系在519. 4 nm处产生一个较强的共振散射峰。当加入三聚氰胺时,多巴胺和三聚氰胺通过氢键结合并形成稳定的多氢键化合物,使得结合的多巴胺还原能力降低而不能还原氯金酸,阻碍金纳米粒子的形成,导致体系519. 4 nm处的共振散射峰强度降低。据此建立了检测三聚氰胺的共振散射光谱法。在优化的实验条件下,c(三聚氰胺)在8. 33×10-7~1. 67×10-5mol/L范围内与该共振散射峰强度的降低值(ΔI519. 4 nm)呈良好线性关系,其相关系数为0. 996 1,检出限为2. 1×10-7mol/L。将该共振散射光谱法用于牛奶制品中的三聚氰胺的测定,回收率为96. 0%~108. 0%,相对标准偏差为1. 3%~2. 7%。
In Na OH solution,gold nanoparticles that exhibited a strong resonance scattering peak at 519. 4 nm were prepared via redox reaction between HAu Cl4 and dopamine.When the melamine was added,dopamine interacted with melamine and formed a stable complex by hydrogen bonds,HAu Cl4 could not be easily reduced by the interacted dopamine,and the formation of gold nanoparticles was interrupted,resulting in the decrease of resonance scattering intensity.Based on this,a resonance scattering spectral method was developed for the determination of melamine.Under the optimal conditions,the decreased resonance scattering intensity( ΔI519. 4 nm) was linear to c( melamine) in the range of 8. 33×10-7~ 1. 67×10-5 mol/L,with a correlation coefficient of 0. 996 1 and a detection limit of 2. 1×10-7 mol/L.This method was utilized to detect melamine in pretreated liquid milk products,with the recoveries of 96. 0% ~ 108. 0% and relative standard deviations of 1. 3% ~ 2. 7%.
In Na OH solution,gold nanoparticles that exhibited a strong resonance scattering peak at 519. 4 nm were prepared via redox reaction between HAu Cl4 and dopamine.When the melamine was added,dopamine interacted with melamine and formed a stable complex by hydrogen bonds,HAu Cl4 could not be easily reduced by the interacted dopamine,and the formation of gold nanoparticles was interrupted,resulting in the decrease of resonance scattering intensity.Based on this,a resonance scattering spectral method was developed for the determination of melamine.Under the optimal conditions,the decreased resonance scattering intensity( ΔI519. 4 nm) was linear to c( melamine) in the range of 8. 33×10-7~ 1. 67×10-5 mol/L,with a correlation coefficient of 0. 996 1 and a detection limit of 2. 1×10-7 mol/L.This method was utilized to detect melamine in pretreated liquid milk products,with the recoveries of 96. 0% ~ 108. 0% and relative standard deviations of 1. 3% ~ 2. 7%.
引文
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[3]林晓珊,吴惠勤,黄晓兰,等.气相色谱-串联质谱法快速测定乳制品中三聚氰胺及其3种类似物[J].质谱学报,2014,35(6):537-542.
[4]SUH J H,JUNG J,KIM B,et al.Development of aqueous mobile phase using chaotrope for the chromatographic determination of melamine in infant formula[J]. J. Chromatogr.A,2017,1 496:174-179.
[5]程定玺,黄世江,梁宇,等.荧光探针法快速测定牛奶中三聚氰胺[J].分析试验室,2013,32(1):48-51.
[6]LU Q J,ZHAO J N,XUE S Y,et al.A turn-on fluorescent sensor for ultrasensitive detection of melamine based on a new fluorescence probe and Au NPs[J]. Analyst,2015,140:1 155-1 160.
[7]孙春燕,张民伟,李宏坤,等.金纳米粒子比色探针检测牛奶及鸡蛋中的三聚氰胺[J].分析化学,2012,40(3):386-390.
[8]MA Y J,JIANG L,MEI Y J,et al. Colorimetric sensing strategy for mercury(Ⅱ)and melamine utilizing cysteamine-modified gold nanoparticles[J]. Analyst,2013,138(18):5 338.
[9]DU X B,CHU H Y,HUANG Y W,et al.Qualitative and quantitative determination of melamine by surface-enhanced Raman spectroscopy using silver nanorod array substrates[J].Appl.Spectrosc.,2010,64(7):781-785.
[10]BETZ J F,CHENG Y,RUBLOFF G W.Direct SERS detection of contaminants in a complex mixture:rapid,single step screening for melamine in liquid infant formula[J].Analyst,2012,137(4):826-828.
[11]LI R P,YANG G H,YANG J L,et al.Determination of melamine in milk using surface plasma effect of aggregated Au@Si O2nanoparticles by SERS technique[J].Food Control,2016,68:14-19.
[12]LIANG A H,ZHAN Y,FAN Y Y,et al. Catalysis of aptamer-modified Au Pd nanoalloy probe and its application to resonance scattering detection of trace UO2+2[J].Nanoscale,2011,3(8):3 178-3 184.
[13]JIANG Z L,ZHOU L P,LIANG A H.Resonance scattering detection of trace melamine using aptamer-modified nanosilver probe as catalyst without separation of its aggregations[J]. Chem. Commun.,2011,47(11):3 162-3 164.
[14]蒋治良,姚冬梅,李芳,等.免疫金铂纳米合金催化共振散射光谱法测定痕量人绒毛膜促性腺激素[J].化学学报,2012,70(16):1 748-1 754.
[15]唐宁莉,张容珲,陈永宁,等.基于纳米银聚集的共振散射光谱法测定痕量多巴胺[J].化学试剂,2016,38(10):971-974.
[16]OUYANG H X,LI C N,LIU Q Y,et al.Resonance rayleigh scattering and SERS spectral detection of trace Hg(Ⅱ)based on the gold nanocatalysis[J].Nanomaterials,2017,7(5):114-123.
[17]林陈银,刘庆业,梁爱惠,等.多氢键反应-纳米金共振散射光谱法测定三聚氰胺[J].分析测试学报,2011,30(11):1 230-1 234.
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