葡萄叶片SPAD值与测定位置及光合色素含量的关系
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摘要
为了实时、准确、无损地检测葡萄叶片中叶绿素的含量水平,以评价葡萄生长发育与营养状况,确保栽培的科学和施肥的合理,以‘夏黑’‘巨玫瑰’‘户太8号’‘阳光玫瑰’‘金手指’‘摩尔多瓦’这6个品种葡萄叶片为试验材料,采用叶绿素测定仪测定了6个葡萄品种叶片不同位置的SPAD值,使用分光光度计测定了葡萄叶片光合色素含量,探寻了葡萄叶片SPAD值的最适测量位点,分析了SPAD值与光合色素含量的关系,并建立了SPAD值与光合色素含量的线性函数拟合方程。结果表明:6个品种葡萄叶片的SPAD值,叶片顶部的均最高,中部的均次之,基部的均最小;其中,葡萄叶片中部的SPAD值最具有代表性。6个品种葡萄叶片的SPAD值与叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量均显著相关,其与叶绿素a含量的相关性最高;在不同品种葡萄叶片的SPAD值与叶绿素a、叶绿素总含量、类胡萝卜素含量的线性函数拟合方程中,‘户太8号’的拟合方程决定系数值最高,其叶绿素a、叶绿素总含量、类胡萝卜素含量的决定系数值分别为0.903 5、0.867 2、0.749 9;在叶绿素b与葡萄叶片SPAD值的拟合方程中,‘金手指’的拟合方程为y=0.034 7 x-0.084 0,其决定系数R2值(0.773 4)最高。单一品种葡萄叶片的SPAD值与光合色素含量的回归模型的拟合准确率比6个葡萄品种汇总拟合回归模型的要高。
In order to accurately and non-destructively detect chlorophyll content in Vitis vinifera leaves in real time,evaluate growth, development and nutritional status in V. vinifera plants, and ensure scientific cultivation and rational fertilization, using leaves in six cultivars of V. vinifera as materials, which were ‘Summer Black', ‘Muscat Kyoho',‘Hutai No.8', ‘Shine Muscat', ‘Gold finger', ‘Moldova', SPAD values in different leaf sites were determined by using chlorophyll meter, photosynthetic pigment contents in leaves by using spectrophotometric method, the optimal determination site for leaf SPAD value, correlation between SPAD value and photosynthetic pigment contents, and linear function fitting equations were established. The results showed that SPAD values were the highest at the top of leaves in all six cultivars of V. vinifera, secondly at the middle, and that were lowest at the basal of leaves. SPAD values at the middle of leaves were the most representative. SPAD values were positively correlated with contents of chlorophyll a,chlorophyll b, total chlorophyll and carotenoid in six cultivars of V. vinifera leaves, and its correlation with chlorophyll a content was the highest. In the fitting equations of linear function of SPAD value with chlorophyll a content, total chlorophyll content and carotenoid content in six cultivars of V. vinifera leaves, determination coefficient values of fitting equation of ‘Hutai No.8' were the highest, and determination coefficient decision values of chlorophyll a content, total chlorophyll content and carotenoid content were 0.903 5, 0.867 2, 0.749 9, respectively. In fitting equations of SPAD value with chlorophyll b content in V. vinifera leaves, fitting equation of ‘Gold Finger' was y = 0.034 7 x-0.084 0, and determination coefficient R2 value(0.7734) was the highest. Fitting accuracy of regression equation of SPAD value with photosynthetic pigment content in single cultivar of V. vinifera leaf was higher than that of total regression equation of six cultivars of V. vinifera leaves.
In order to accurately and non-destructively detect chlorophyll content in Vitis vinifera leaves in real time,evaluate growth, development and nutritional status in V. vinifera plants, and ensure scientific cultivation and rational fertilization, using leaves in six cultivars of V. vinifera as materials, which were ‘Summer Black', ‘Muscat Kyoho',‘Hutai No.8', ‘Shine Muscat', ‘Gold finger', ‘Moldova', SPAD values in different leaf sites were determined by using chlorophyll meter, photosynthetic pigment contents in leaves by using spectrophotometric method, the optimal determination site for leaf SPAD value, correlation between SPAD value and photosynthetic pigment contents, and linear function fitting equations were established. The results showed that SPAD values were the highest at the top of leaves in all six cultivars of V. vinifera, secondly at the middle, and that were lowest at the basal of leaves. SPAD values at the middle of leaves were the most representative. SPAD values were positively correlated with contents of chlorophyll a,chlorophyll b, total chlorophyll and carotenoid in six cultivars of V. vinifera leaves, and its correlation with chlorophyll a content was the highest. In the fitting equations of linear function of SPAD value with chlorophyll a content, total chlorophyll content and carotenoid content in six cultivars of V. vinifera leaves, determination coefficient values of fitting equation of ‘Hutai No.8' were the highest, and determination coefficient decision values of chlorophyll a content, total chlorophyll content and carotenoid content were 0.903 5, 0.867 2, 0.749 9, respectively. In fitting equations of SPAD value with chlorophyll b content in V. vinifera leaves, fitting equation of ‘Gold Finger' was y = 0.034 7 x-0.084 0, and determination coefficient R2 value(0.7734) was the highest. Fitting accuracy of regression equation of SPAD value with photosynthetic pigment content in single cultivar of V. vinifera leaf was higher than that of total regression equation of six cultivars of V. vinifera leaves.
引文
[1]姜瑛,刘晔,张林利,等.不同质地潮土冬小麦SPAD值在氮素营养诊断上的应用研究[J].河南农业大学学报,2018,52(4):485-491.
[2]李杰,冯跃华,牟桂婷,等.基于SPAD值的水稻施氮叶值模型构建及应用效果[J].中国农业科学,2017,50(24):4714-4724.
[3]郭爱霞,王延秀,唐健,等.不同复合铁肥对石灰质土壤苹果黄化病的矫正效果[J].甘肃农业大学学报,2018,53(3):76-82.
[4]薛进军,田自武,崔美香,等.铁肥管道输液滴干对苹果铁营养的影响[J].果树学报,2017,34(12):1537-1544.
[5]马宗桓,陈佰鸿,毛娟,等.施氮时期对酿酒葡萄叶片氮代谢酶及相关基因表达的影响[J].西北植物学报,2018,38(2):298-306.
[6]鲁春艳,陈晓艳,郭改改,等.秋季叶面喷肥对红地球葡萄生长及果实品质的影响[J].西北农林科技大学学报(自然科学版),2016,44(2):137-143.
[7]潘可可.肥料减量施用对设施番茄产量、品质的影响[D].南京:南京农业大学,2010:4-7.
[8]周兴,王振平,代红军.不同施肥处理对蛇龙珠葡萄光合性能及品质的影响[J].北方园艺,2013(14):1-4.
[9]史力超,翟勇,王雪艳,等.基于叶片SPAD值的滴灌春小麦氮肥分期施用推荐模型[J].干旱地区农业研究, 2017,35(1):103-107.
[10]卢晓萍,杨丙贤,徐婵娟,等.3种小檗科植物叶片SPAD值与叶绿素的相关性及通径分析[J].浙江大学学报(农业与生命科学版),2013,39(3):261-266.
[11]鱼欢,祖超,杨建峰,等.应用SPAD叶绿素仪测定不同位置胡椒叶片的SPAD值[J].热带作物学报,2012,33(10):1890-1895.
[12]高飞,肖靖,谷运红,等.利用小麦叶片SPAD值预测成熟期籽粒蛋白质含量的研究[J].光谱学与光谱分析,2012,32(5):1350-1354.
[13]杨虎,戈长水,应武,等.遮荫对水稻冠层叶片SPAD值及光合、形态特性参数的影响[J].植物营养与肥料学报,2014,20(3):580-587.
[14]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[15]杨益花,李庆魁,苏祖芳,等.拔节、抽穗期叶片SPAD值与杂交中籼稻产量及其构成因素的关系[J].栽培与耕作,2006(4):12-14.
[16]胡昊,白由路,杨俐苹,等.基于SPAD-502与Green Seeker的冬小麦氮营养诊断研究[J].中国生态农业学报,2010,18(4):748-752.
[17]赵士诚,何萍,仇少君,等.相对SPAD值用于不同品种夏玉米氮肥管理的研究[J].植物营养与肥料学报,2011,17(5):1091-1098.
[18]曹兰芹,伍晓明,杨睿,等.不同氮吸收效率品种油菜氮素营养特性的差异[J].作物学报,2012,38(5):887-895.
[19]王丽丽,陈敏,栾炳辉,等.葡萄叶片厚度和茸毛密度与其对绿盲蝽抗性的关系[J].果树学报, 2017,34(2):238-244.
[20]MCGILLBJ,ENQUISTBJ, WEIHERE,etal.Rebuilding community ecology from functional traits[J].Trends in Ecology&Evolution, 2006,21(4):178-185.
[21]KATTGE J, DíAZ S, LAVOREL S, et al. TRY-A global database of plant traits[J]. Global Change Biology, 2011,17(9):2905-2935.
[22]黎冰,侍朋宝,栾雪涛,等.硝铵态氮不同配比对葡萄新梢生长和叶片光合特性的影响[J].西北农林科技大学学报(自然科学版),2018,46(2):73-83.
[23]刘旻霞.甘南高寒草甸植物元素含量与土壤因子对坡向梯度的响应[J].生态学报,2017,37(24):8275-8284.
[24]王瑞,陈永忠,陈隆升,等.油茶叶片SPAD值与叶绿素含量的相关分析[J].中南林业科技大学学报,2013,33(2):77-80.
[25]李田,韩霞,肖龙,等.桃树叶片叶绿素含量与SPAD值的相关性分析[J].经济林研究,2018,36(4):99-103.
[26]陈景蕊,潘静.不同品种葡萄叶片SPAD值与叶绿素含量相关性分析[J].北方园艺,2015(19):42-46.
[2]李杰,冯跃华,牟桂婷,等.基于SPAD值的水稻施氮叶值模型构建及应用效果[J].中国农业科学,2017,50(24):4714-4724.
[3]郭爱霞,王延秀,唐健,等.不同复合铁肥对石灰质土壤苹果黄化病的矫正效果[J].甘肃农业大学学报,2018,53(3):76-82.
[4]薛进军,田自武,崔美香,等.铁肥管道输液滴干对苹果铁营养的影响[J].果树学报,2017,34(12):1537-1544.
[5]马宗桓,陈佰鸿,毛娟,等.施氮时期对酿酒葡萄叶片氮代谢酶及相关基因表达的影响[J].西北植物学报,2018,38(2):298-306.
[6]鲁春艳,陈晓艳,郭改改,等.秋季叶面喷肥对红地球葡萄生长及果实品质的影响[J].西北农林科技大学学报(自然科学版),2016,44(2):137-143.
[7]潘可可.肥料减量施用对设施番茄产量、品质的影响[D].南京:南京农业大学,2010:4-7.
[8]周兴,王振平,代红军.不同施肥处理对蛇龙珠葡萄光合性能及品质的影响[J].北方园艺,2013(14):1-4.
[9]史力超,翟勇,王雪艳,等.基于叶片SPAD值的滴灌春小麦氮肥分期施用推荐模型[J].干旱地区农业研究, 2017,35(1):103-107.
[10]卢晓萍,杨丙贤,徐婵娟,等.3种小檗科植物叶片SPAD值与叶绿素的相关性及通径分析[J].浙江大学学报(农业与生命科学版),2013,39(3):261-266.
[11]鱼欢,祖超,杨建峰,等.应用SPAD叶绿素仪测定不同位置胡椒叶片的SPAD值[J].热带作物学报,2012,33(10):1890-1895.
[12]高飞,肖靖,谷运红,等.利用小麦叶片SPAD值预测成熟期籽粒蛋白质含量的研究[J].光谱学与光谱分析,2012,32(5):1350-1354.
[13]杨虎,戈长水,应武,等.遮荫对水稻冠层叶片SPAD值及光合、形态特性参数的影响[J].植物营养与肥料学报,2014,20(3):580-587.
[14]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[15]杨益花,李庆魁,苏祖芳,等.拔节、抽穗期叶片SPAD值与杂交中籼稻产量及其构成因素的关系[J].栽培与耕作,2006(4):12-14.
[16]胡昊,白由路,杨俐苹,等.基于SPAD-502与Green Seeker的冬小麦氮营养诊断研究[J].中国生态农业学报,2010,18(4):748-752.
[17]赵士诚,何萍,仇少君,等.相对SPAD值用于不同品种夏玉米氮肥管理的研究[J].植物营养与肥料学报,2011,17(5):1091-1098.
[18]曹兰芹,伍晓明,杨睿,等.不同氮吸收效率品种油菜氮素营养特性的差异[J].作物学报,2012,38(5):887-895.
[19]王丽丽,陈敏,栾炳辉,等.葡萄叶片厚度和茸毛密度与其对绿盲蝽抗性的关系[J].果树学报, 2017,34(2):238-244.
[20]MCGILLBJ,ENQUISTBJ, WEIHERE,etal.Rebuilding community ecology from functional traits[J].Trends in Ecology&Evolution, 2006,21(4):178-185.
[21]KATTGE J, DíAZ S, LAVOREL S, et al. TRY-A global database of plant traits[J]. Global Change Biology, 2011,17(9):2905-2935.
[22]黎冰,侍朋宝,栾雪涛,等.硝铵态氮不同配比对葡萄新梢生长和叶片光合特性的影响[J].西北农林科技大学学报(自然科学版),2018,46(2):73-83.
[23]刘旻霞.甘南高寒草甸植物元素含量与土壤因子对坡向梯度的响应[J].生态学报,2017,37(24):8275-8284.
[24]王瑞,陈永忠,陈隆升,等.油茶叶片SPAD值与叶绿素含量的相关分析[J].中南林业科技大学学报,2013,33(2):77-80.
[25]李田,韩霞,肖龙,等.桃树叶片叶绿素含量与SPAD值的相关性分析[J].经济林研究,2018,36(4):99-103.
[26]陈景蕊,潘静.不同品种葡萄叶片SPAD值与叶绿素含量相关性分析[J].北方园艺,2015(19):42-46.
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