咀嚼槟榔对大鼠口腔黏膜鳞状上皮细胞的影响
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摘要
目的:观察咀嚼槟榔对大鼠口腔黏膜鳞状上皮细胞的影响。方法:将82只SD大鼠随机分成4组,其中对照组(CON组) 10只,槟榔提取液涂抹组(T组) 24只,机械刺激组(S组) 24只,槟榔提取液涂抹+机械刺激组(T+S组) 24只,干预10周后自由饮食饲养2周。观察各组大鼠干预6、8、10、12周的一般状态及口腔黏膜鳞状上皮细胞的变化。结果:槟榔化学成分及粗纤维均可损害口腔黏膜,造成大鼠口腔黏膜上皮破坏、增生,但其严重程度T+S组> T组≈S组> CON组。结论:与单一刺激相比,采用化学刺激与机械相结合方法可诱发出较典型、稳定的大鼠口腔黏膜鳞状上皮损伤。
Objective: To investigate the influence of areca nut chewing on squamous epithelial cells of oral mucosa in rats. Methods: A total of 82 Sprague-Dawley rats were randomly divided into control group( CON group) with 10 rats,areca nut extract group( T group) with 24 rats,mechanical stimulation group( S group) with24 rats,and areca nut extract + mechanical stimulation group( T + S group) with 24 rats. Intervention was performed for 10 weeks,followed by 2 weeks of feeding with free diet. The rats were observed in terms of the changes in general condition and squamous epithelial cells of oral mucosa at weeks 6,8,10,and 12 of intervention. Results: The oral mucosa was injured by the chemical components and coarse fibers of areca nut,resulting in the damage and proliferation of oral mucosa epithelium,which was most severe in the T + S group,followed by the T group/the S group( similar) and the CON group. Conclusion: Compared with single stimulation,chemical stimulation combined with mechanical stimulation can induce typical and stable squamous epithelial injury of oral mucosa in rats.
Objective: To investigate the influence of areca nut chewing on squamous epithelial cells of oral mucosa in rats. Methods: A total of 82 Sprague-Dawley rats were randomly divided into control group( CON group) with 10 rats,areca nut extract group( T group) with 24 rats,mechanical stimulation group( S group) with24 rats,and areca nut extract + mechanical stimulation group( T + S group) with 24 rats. Intervention was performed for 10 weeks,followed by 2 weeks of feeding with free diet. The rats were observed in terms of the changes in general condition and squamous epithelial cells of oral mucosa at weeks 6,8,10,and 12 of intervention. Results: The oral mucosa was injured by the chemical components and coarse fibers of areca nut,resulting in the damage and proliferation of oral mucosa epithelium,which was most severe in the T + S group,followed by the T group/the S group( similar) and the CON group. Conclusion: Compared with single stimulation,chemical stimulation combined with mechanical stimulation can induce typical and stable squamous epithelial injury of oral mucosa in rats.
引文
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[3] Maria S,Kamath VV,Satelur K,et al. Evaluation of transforming growth factor beta1 gene in oral submucous fibrosis induced in Sprague-Dawley rats by injections of areca nut and pan masala(commercial areca nut product)extracts[J]. Journal of cancer research and therapeutics,2016,12(1):379-385.
[4]黄龙,翦新春.槟榔致癌物质与口腔癌[J].国际口腔医学杂志,2014,41(1):102-107.
[5]田媛,金武龙.口腔癌动物模型的建立及研究进展[J].临床口腔医学杂志,2013,29(12):756-757.
[6]黄生高,凌天牖,吴汉江,等.槟榔提取液诱发大鼠口腔黏膜下纤维性变的实验研究Ⅰ.组织形态学观察[J].华西口腔医学杂志,1997,15(2):91-96.
[7]陈梁,周海文.口腔癌的鼠科动物模型研究进展[J].临床口腔医学杂志,2011,27(8):508-511.