女宝胶囊气相特征图谱
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摘要
目的:利用气相色谱法建立女宝胶囊特征图谱的质量评价方法。方法:采用气相色谱;DB-FFAP毛细管柱(柱长为30 m,内径为0.25 mm,膜厚度为0.25μm);程序升温:初始温度为100℃,保持15 min,以8℃·min~(-1)的速率升温至220℃,保持30 min;进样口温度为240℃;检测器温度为260℃;载气为氮气,流速为1.0 mL·min~(-1)。结果:建立的特征图谱信息丰富,6味含有挥发油类中药材中的16个特征峰分离良好,且重复性和稳定性的RSD均小于1.0%。结论:本研究所建立的方法快速、可靠,且专属性强,适用于全面控制女宝胶囊的质量。
Objective:To establish a specific GC method for quality evaluation of Nvbao Capsules.Methods:GC with DB-FFAP quartz capillary(Length 30 m,inner diameter 0.25 mm,membrane thickness 0.25 μm)was adopted in this study.The GC conditions were set as follows:the initial temperature of 100 ℃ was maintained for 15 minutes,raised to 220 ℃ with the heating rate of 8 ℃·min~(-1) maintained for 30 minutes.The temperature of inlet and detector were set at 240 ℃ and 260 ℃,respectively.Nitrogen was used as the carrier gas at the flow rate of 1.0 mL·min~(-1).Results:The information of specific chromatogram was abundant,16 characteristic peaks belong to 6 kinds of medicinal materials rich in volatile oil were separated well,and RSD of repeatability and stability were less than 1.0%.Conclusion:The method is fast,simple and reliable,which can be used for the comprehensive quality control of Nvbao Capsules.
Objective:To establish a specific GC method for quality evaluation of Nvbao Capsules.Methods:GC with DB-FFAP quartz capillary(Length 30 m,inner diameter 0.25 mm,membrane thickness 0.25 μm)was adopted in this study.The GC conditions were set as follows:the initial temperature of 100 ℃ was maintained for 15 minutes,raised to 220 ℃ with the heating rate of 8 ℃·min~(-1) maintained for 30 minutes.The temperature of inlet and detector were set at 240 ℃ and 260 ℃,respectively.Nitrogen was used as the carrier gas at the flow rate of 1.0 mL·min~(-1).Results:The information of specific chromatogram was abundant,16 characteristic peaks belong to 6 kinds of medicinal materials rich in volatile oil were separated well,and RSD of repeatability and stability were less than 1.0%.Conclusion:The method is fast,simple and reliable,which can be used for the comprehensive quality control of Nvbao Capsules.
引文
[1] 国家药典委员会.中华人民共和国卫生部药品标准:第五册[M].北京:化学工业出版社,1989:18.
[2] 刘敏,李水福.HPLC同时测定女宝胶囊中丹皮酚和丹参酮ⅡA含量的方法学研究[J].中华中医药学刊,2011,29(6):1407-1409.
[3] 邹录惠,邱莉.SPE-HPLC-UV法测定山楂药材特征图谱研究[J].药物分析杂志,2014,34(9):1597-1600.
[4] 张永鑫,刘晓.黄连4种饮片特征图谱研究及生物碱含量测定[J].药物分析杂志,2012,32(11):1957-1961.
[5] 马培,许利嘉.虎杖药材UPLC特征图谱研究[J].中国实验方剂学杂志,2012,18(2):72-76.
[6] 宋霞,林鹏程.明目蒺藜丸中挥发性成分的GC特征图谱及有效成分的含量测定[J].中药材,2017,40(8):1880-1883.
[7] 任乔森,王海洋.朝鲜白头翁的HPLC特征图谱研究[J].中国现代中药,2018,20(6):711-715.
[8] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:168.
[9] 郭美玲,冯振斌.通窍耳聋丸UPLC特征图谱的研究[J].中国药品标准,2016,17(6):415-417.
[2] 刘敏,李水福.HPLC同时测定女宝胶囊中丹皮酚和丹参酮ⅡA含量的方法学研究[J].中华中医药学刊,2011,29(6):1407-1409.
[3] 邹录惠,邱莉.SPE-HPLC-UV法测定山楂药材特征图谱研究[J].药物分析杂志,2014,34(9):1597-1600.
[4] 张永鑫,刘晓.黄连4种饮片特征图谱研究及生物碱含量测定[J].药物分析杂志,2012,32(11):1957-1961.
[5] 马培,许利嘉.虎杖药材UPLC特征图谱研究[J].中国实验方剂学杂志,2012,18(2):72-76.
[6] 宋霞,林鹏程.明目蒺藜丸中挥发性成分的GC特征图谱及有效成分的含量测定[J].中药材,2017,40(8):1880-1883.
[7] 任乔森,王海洋.朝鲜白头翁的HPLC特征图谱研究[J].中国现代中药,2018,20(6):711-715.
[8] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:168.
[9] 郭美玲,冯振斌.通窍耳聋丸UPLC特征图谱的研究[J].中国药品标准,2016,17(6):415-417.