杂交相思组织培养研究初报
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摘要
【目的】筛选杂交相思组织培养不同培养阶段的培养基配方,为实现杂交相思工厂化育苗提供技术参考。【方法】用杂交相思嫩茎为外植体,探究生长调节剂6-BA、NAA、IBA对其诱导出芽、继代增殖和诱导生根的效果。【结果】在不添加6-BA的情况下,添加0.2mg/LNAA时芽诱导率为60.0%,当添加0.2mg/L的6-BA后,诱导率为42.0%。当NAA为0~0.5mg/L时,随着6-BA的增加,增殖系数随之增加,6-BA为0.8mg/L时,增殖系数为4.2;当6-BA为1.0mg/L以上时,继代苗的增殖系数为1.0。以改良MS为基本培养基,在NAA1.0mg/L的条件下添加0.5、1.0mg/L的IBA生根诱导率分别为17.5%、24.0%;以改良1/2MS为基本培养基,在NAA1.0mg/L的条件下,添加0.5mg/L的IBA生根率为91.7%,当添加IBA为1.0mg/L时,生根率降到50.0%;生根苗移栽基质以黄心土与河沙或蛭石混合较好,移栽成活率在85.0%以上。【结论】改良MS+NAA0.2mg/L培养基是较适宜芽诱导的培养基;改良MS+6-BA0.8mg/L+NAA0.5mg/L培养基较利于继代苗的生长;改良1/2MS+NAA1.0mg/L+IBA0.5mg/L培养基较改良MS培养基有利于诱导生根。
[Objective]The rapid propagation technique of Acacia auriculaeformis×Acacia mangium was studied to screen proper culture medium formulation for different stages and to achieve industrializing breeding. [Method]Using hybrid Acacia stems as explants for tissue culture, effects of growth regulators including 6-BA, NAA and IBA of different concentrations on induced sprouting, subculture propagation and root induction were explored. [Result]The results showed that in case of 0.2 mg/ L NAA, the initial induction rate was 60.0% when no 6-BA was added, and the induction rate was 42.0% when there was0.2 mg/L 6-BA. At 0- 0.5 mg/L NAA, proliferation coefficient increased with the increase of 6-BA concentration. At 0.8 mg/L 6-BA, the propagation coefficient was 4.2. When 6-BA was above 1.0 mg/L, transgenerational seedling proliferation coefficient was 1.0. Using the improved MS as the basic medium, under the condition of 1.0 mg/L NAA, rooting induction rate was 17.5% and 24.0% for 0.5 and 1.0 mg/L IBA, respectively. Using the 1/2 modified MS as the basic medium, under the condition of 1.0 mg/L NAA, rooting rate was 91.7% and 50% for 0.5 mg/L IBA and 1.0 mg/L IBA, respectively. When rooting seedlings were transplanted in substrate with yellow subsoil and river sand or vermiculite mixture, transplanting survival rate was more than 85.0%. [Conclusion]Improved MS +NAA 0.2 mg/L medium was more appropriate for the initial induction medium. Improved MS+6-BA 0.8 mg/ L+NAA 0.5 mg/L was conducive to the growth of transgenerational seedlings. 1/2 modified MS+NAA 1.0 mg/L+IBA 0.5 mg/L was better for root induction.
[Objective]The rapid propagation technique of Acacia auriculaeformis×Acacia mangium was studied to screen proper culture medium formulation for different stages and to achieve industrializing breeding. [Method]Using hybrid Acacia stems as explants for tissue culture, effects of growth regulators including 6-BA, NAA and IBA of different concentrations on induced sprouting, subculture propagation and root induction were explored. [Result]The results showed that in case of 0.2 mg/ L NAA, the initial induction rate was 60.0% when no 6-BA was added, and the induction rate was 42.0% when there was0.2 mg/L 6-BA. At 0- 0.5 mg/L NAA, proliferation coefficient increased with the increase of 6-BA concentration. At 0.8 mg/L 6-BA, the propagation coefficient was 4.2. When 6-BA was above 1.0 mg/L, transgenerational seedling proliferation coefficient was 1.0. Using the improved MS as the basic medium, under the condition of 1.0 mg/L NAA, rooting induction rate was 17.5% and 24.0% for 0.5 and 1.0 mg/L IBA, respectively. Using the 1/2 modified MS as the basic medium, under the condition of 1.0 mg/L NAA, rooting rate was 91.7% and 50% for 0.5 mg/L IBA and 1.0 mg/L IBA, respectively. When rooting seedlings were transplanted in substrate with yellow subsoil and river sand or vermiculite mixture, transplanting survival rate was more than 85.0%. [Conclusion]Improved MS +NAA 0.2 mg/L medium was more appropriate for the initial induction medium. Improved MS+6-BA 0.8 mg/ L+NAA 0.5 mg/L was conducive to the growth of transgenerational seedlings. 1/2 modified MS+NAA 1.0 mg/L+IBA 0.5 mg/L was better for root induction.
引文
蔡玲,王以红,吴幼媚,苏烜.2003.五种相思树组织培养研究[J].广西林业科学,32(1):24-26.Cai L,Wang Y H,Wu Y M,Su X.2003.Tissue culture of five Acacia species[J].Guangxi Forestry Science,32(1):24-26.
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赖家业,周传明,叶春生,黄寿先,韦鹏霄,岑秀芬,陈放.2003.厚荚相思组织培养与快速繁殖[J].四川大学学报:自然科学版,40(5):982-985.Lai J Y,Zhou C M,Ye C S,Huang S X,Wei P X,Chen X F,Chen F.2003.In vitro micropropagation of Acacia crassicarpa[J].Journal of Sichuan University:Natural Science Edition,40(5):982-985.
潘月芳,郝海坤,钟瑜,曹艳云.2009.越南杂交相思容器育苗的基质选择[J].林业科技开发,23(4):105-107.Pan Y F,Hao H K,Zhong Y,Cao Y Y.2009.The selection of substrate for container seedlings of Acacia mangium×A.acuriculaeformis[J].China Forestry Science and Technolo-gy,23(4):105-107.
潘志刚,吕鹏信,杨民权,曾育田,李昌明,吴鸿琼.1988.五种热带相思三年种源试验初报[J].林业科学研究,1(5):553-559.Pan Z G,LüP X,Yang M Q,Zeng Y T,Li C M,Wu H Q.1988.Preliminary report on the 3-year-old provenance testof tropical acacias in China[J].Forest Research,1(5):553-559.
肖木兴.2007.杉木组培苗移栽技术[J].林业科技开发,21(6):103-104.Xiao M X.2007.Tissue culture seedling transplanting technology of Chinese fi[rJ].China Forestry Science and Technology,21(6):103-104.
熊建勇,宗亦臣,常金财,郑勇奇,吴红芝.2011.马大相思茎段的组织培养[J].林业实用技术,(9):29-30.Xiong J Y,Zong Y C,Chang J C,Zheng Y Q,Wu H Z.2011.Tissue culture of stem segments of Martha Acacia[J].Practical Forestry Technology,(9):29-30.
周传明,梁机,黄勇,熊英,莫雅芳.2007.厚荚相思扦插育苗试验[J].广西林业科学,36(1):36-38.Zhou C M,Liang J,Huang Y,Xiong Y,Mo Y F.2007.Experiments on rooted cutting of seedling of Acacia crassicarpa[J].Guangxi Forestry Science,36(1):36-38.
曹艳云,潘月芳,郝海坤,彭玉华,黄志玲.2006.杂交相思扦插繁殖试验[J].广西林业科学,35(3):151-152.Cao Y Y,Pan Y F,Hao H K,Peng Y H,Huang Z L.2006.Cuttings propagation trial of Acacia auriculiformis×A.mangium[J].Guangxi Forestry Science,35(3):151-152.
曹艳云,彭玉华,郝海坤,何琴飞,周玉端,黄志玲.2011.杂交相思母本苗高对扦插效果的影响[J].西部林业科学,40(4):28-31.Cao Y Y,Peng Y H,Hao H K,He Q F,Zhou Y D,Huang ZL.2011.Effects of different grade maternal plants on cutting propagation of A.mangium×A.acuriculaeformis[J].Journal of West China Forestry Science,40(4):28-31.
陈诚,蒲俊文,姚胜,姜亦飞.2009.速生阔叶材杂交相思制浆性能的研究[J].造纸科学与技术,28(4):1-3.Chen C,Pu J W,Yao S,Jiang Y F.2009.Study on the pulping performances of fast growing wood A.acuriculaeformis×A.mangium[J].Paper Science & Technology,28(4):1-3.
赖家业,周传明,叶春生,黄寿先,韦鹏霄,岑秀芬,陈放.2003.厚荚相思组织培养与快速繁殖[J].四川大学学报:自然科学版,40(5):982-985.Lai J Y,Zhou C M,Ye C S,Huang S X,Wei P X,Chen X F,Chen F.2003.In vitro micropropagation of Acacia crassicarpa[J].Journal of Sichuan University:Natural Science Edition,40(5):982-985.
潘月芳,郝海坤,钟瑜,曹艳云.2009.越南杂交相思容器育苗的基质选择[J].林业科技开发,23(4):105-107.Pan Y F,Hao H K,Zhong Y,Cao Y Y.2009.The selection of substrate for container seedlings of Acacia mangium×A.acuriculaeformis[J].China Forestry Science and Technolo-gy,23(4):105-107.
潘志刚,吕鹏信,杨民权,曾育田,李昌明,吴鸿琼.1988.五种热带相思三年种源试验初报[J].林业科学研究,1(5):553-559.Pan Z G,LüP X,Yang M Q,Zeng Y T,Li C M,Wu H Q.1988.Preliminary report on the 3-year-old provenance testof tropical acacias in China[J].Forest Research,1(5):553-559.
肖木兴.2007.杉木组培苗移栽技术[J].林业科技开发,21(6):103-104.Xiao M X.2007.Tissue culture seedling transplanting technology of Chinese fi[rJ].China Forestry Science and Technology,21(6):103-104.
熊建勇,宗亦臣,常金财,郑勇奇,吴红芝.2011.马大相思茎段的组织培养[J].林业实用技术,(9):29-30.Xiong J Y,Zong Y C,Chang J C,Zheng Y Q,Wu H Z.2011.Tissue culture of stem segments of Martha Acacia[J].Practical Forestry Technology,(9):29-30.
周传明,梁机,黄勇,熊英,莫雅芳.2007.厚荚相思扦插育苗试验[J].广西林业科学,36(1):36-38.Zhou C M,Liang J,Huang Y,Xiong Y,Mo Y F.2007.Experiments on rooted cutting of seedling of Acacia crassicarpa[J].Guangxi Forestry Science,36(1):36-38.