湖北钉螺与中华圆田螺体内酚氧化酶活力比较
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摘要
目的比较湖北钉螺及中华圆田螺体内酚氧化酶活力差异。方法以螺软体组织制备粗制酶液,以邻苯二酚为底物,测定肋壳钉螺、光壳钉螺和中华圆田螺体内酚氧化酶活力。结果光壳钉螺、肋壳钉螺、中华圆田螺体内酚氧化酶活力值分别为(25.72±2.27)、(14.56±1.24)U/mL和(13.72±1.06)U/mL,光壳钉螺体内酚氧化酶活力值高于肋壳钉螺(q=21.46,P <0.05)及中华圆田螺(q=12.00,P <0.05),中华圆田螺与肋壳钉螺体内酚氧化酶活力值差异无统计学意义(q=1.62,P> 0.05)。结论湖北钉螺及中华圆田螺体内酚氧化酶活力存在差异,可能与螺的生活环境有关。
Objective To study the differences of the phenoloxidase(PO)relative activity among ribbed shelled Oncomelania hupensis,smooth shelled O. hupensis and Cipangopaludina chinensis. Methods The crude PO fluid was extracted from the soft tissue of O. hupensis and C. chinensis by homogenation and centrifugation. The PO activity was detected with catechol as the substrate in the reaction systems. Results The PO relative activities in the ribbed shelled O. hupensis,smooth shelled O. hupensis and C. chinensis were(25.72 ± 2.27),(14.56 ± 1.24)U/mL and(13.72 ± 1.06)U/mL. The PO relative activity in the smooth shelled O. hupensis was higher than that in the ribbed shelled O. hupensis(q = 21.46,P < 0.05)and C. chinensis(q =12.00,P < 0.05),while the difference between the PO relative activities of the latter two was not statistically significant(q =1.62,P > 0.05). Conclusion There is a difference in the relative PO activity between O. hupensis and C. chinensis,which may be related to the living environment of snails.
Objective To study the differences of the phenoloxidase(PO)relative activity among ribbed shelled Oncomelania hupensis,smooth shelled O. hupensis and Cipangopaludina chinensis. Methods The crude PO fluid was extracted from the soft tissue of O. hupensis and C. chinensis by homogenation and centrifugation. The PO activity was detected with catechol as the substrate in the reaction systems. Results The PO relative activities in the ribbed shelled O. hupensis,smooth shelled O. hupensis and C. chinensis were(25.72 ± 2.27),(14.56 ± 1.24)U/mL and(13.72 ± 1.06)U/mL. The PO relative activity in the smooth shelled O. hupensis was higher than that in the ribbed shelled O. hupensis(q = 21.46,P < 0.05)and C. chinensis(q =12.00,P < 0.05),while the difference between the PO relative activities of the latter two was not statistically significant(q =1.62,P > 0.05). Conclusion There is a difference in the relative PO activity between O. hupensis and C. chinensis,which may be related to the living environment of snails.
引文
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[17]Le Clec'h W,Anderson TJ,Chevalier FD.Characterization of hemolymph phenoloxidase activity in two Biomphalaria snail species and impact of Schistosoma mansoni infection[J].Parasit Vectors,2016,9:32.
[2]王鑫磊,李倩,张莉,等.钉螺生物学特性及人工培养研究进展[J].中国病原生物学杂志,2015,10(12):1148-1150.
[3]杨进孙,唐小牛,周书林.湖北钉螺酚氧化酶的部分纯化及酶学特征[J].中国人兽共患病学报,2008,24(1):55-57.
[4]徐广增,李伟,王新红,等.斜纹夜蛾酚氧化酶酶学特性研究[J].植物保护,2015,41(3):45-49,63.
[5]徐亚玲,李文楚.昆虫酚氧化酶作用机制的研究进展[J].安徽农业科学,2010,38(27):14844-14846.
[6]吴曙,王淑红,王艺磊,等.软体动物和甲壳动物酚氧化酶的研究进展[J].动物学杂志,2009,44(5):137-146.
[7]Wang WF,Yang H,Liu F,et al.A novel effect of imidazole derivative KK-42 on increasing survival of Aeromonas hydrophila challenged prawn Macrobrachium nipponense[J].Fish Shellfish Immunol,2013,34(1):167-172.
[8]Verghese B,Radhakrishnan EV,Padhi A.Effect of environmental parameters on immune response of the Indian spiny lobster,Panulirus homarus(Linnaeus,1758)[J].Fish Shellfish Immunol,2007,23(5):928-936.
[9]Cheng WT,Wang L,Chen JC.Effect of water temperature on the immune response of white shrimp Litopenaeus vannamei to Vibrio alginolyticus[J].Aquaculture,2005,250(3/4):592-601.181
[10]Bhunia AS,Mukherjee S,Bhunia NS,et al.Immunological resilience of a freshwater Indian mollusc during aestivation and starvation[J].Aquac Rep,2016,3:1-11.
[11]Mukherjee S,Bhunia AS,Bhunia NS,et al.Immunomodulatory effects of temperature and p H of water in an Indian freshwater sponge[J].J Therm Biol,2016,59:1-12.
[12]Lamela REL,Silveira Coffigny R,Quintana YC,et al.Phenoloxidase and peroxidase activity in the shrimp Litopenaeus schmitti,Perez-Farfante and Kensley(1997)exposed to low salinity[J].Aquac Res,2005,36(13):1293-1297.
[13]Vargas-Albores F,Hinojosa-Baltazar P,Portillo-Clark G,et al.Influence of temperature and salinity on the yellowleg shrimp,Penaeus californiensis Holmes,prophenoloxidase system[J].Aquac Res,1998,29(8):549-553.170
[14]潘鲁青.环境因子对甲壳动物渗透调节与免疫力的影响[D].青岛:中国海洋大学,2004.
[15]Jiang J,Zhou Z,Dong Y,et al.Characterization of phenoloxidase from the sea cucumber Apostichopus japonicus[J].Immunobiology,2014,219(6):450-456.
[16]Raynova Y,Doumanova L,Idakieva KN.Phenoloxidase activity of Helix aspersa maxima(garden snail,gastropod)hemocyanin[J].Protein J,2013,32(8):609-618.
[17]Le Clec'h W,Anderson TJ,Chevalier FD.Characterization of hemolymph phenoloxidase activity in two Biomphalaria snail species and impact of Schistosoma mansoni infection[J].Parasit Vectors,2016,9:32.
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