3种微柱凝胶抗人球蛋白试剂卡的检测差异比较
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摘要
目的:探讨3种微柱凝胶抗人球蛋白试剂卡的检测效力,评价其灵敏度差异,为临床试验选择合适的试剂卡提供理论依据。方法:随机选取本院交叉配血次侧不合住院病人标本,运用直接抗人球蛋白试验方法对3种试剂卡进行平行检测。结果:3种试剂卡检出率比较存在显著性差异(P <0. 05),A、B卡与C卡比较均存在显著性差异(P <0. 05)但A卡与B卡比较无显著性差异(P> 0. 05)。对于A、B卡检测为弱阳性的标本,用C卡检测普遍为阴性。对于弱阳性标本,凝集强度A卡> B卡> C卡;对于强阳性标本,凝集强度B卡> A卡> C卡。检测灵敏度B卡> A卡> C卡。结论:推荐临床各实验室使用B卡对患者标本进行输血相容性检测,辅以A卡与C卡进行比较和排异。
Objective: To investigate the detection efficacy of three kinds of microcolumn gel antihuman globulin test kits,and to evaluate their sensitivity differences,so as to provide a theoretical basis for selecting suitable test kits in clinical trials.Methods: Samples of inpatients with incompatible secondary side of cross matching were randomly selected,and three test kits received parallel detection by direct anti-human globulin test. Results: There were significant differences in the detection rate of three test kits( P < 0. 05),and there were significant differences between A,B kits and C kits( P < 0. 05),but there was no significant difference between A and B kits( P > 0. 05). For weakly positive specimens detected by A and B kits,C kit was generally negative; for weakly positive specimens,agglutination strength ranking was A kit > B kit > C kit; for strongly positive specimens,agglutination strength ranking was B kit > A kit > C kit. Detection sensitivity ranking was B kit > A kit > C kit.Conclusion: It is recommended that clinical laboratories use B kit to test the blood transfusion compatibility of patients' specimens,which is supplemented by A kit and C kit for the comparison and exclusion.
Objective: To investigate the detection efficacy of three kinds of microcolumn gel antihuman globulin test kits,and to evaluate their sensitivity differences,so as to provide a theoretical basis for selecting suitable test kits in clinical trials.Methods: Samples of inpatients with incompatible secondary side of cross matching were randomly selected,and three test kits received parallel detection by direct anti-human globulin test. Results: There were significant differences in the detection rate of three test kits( P < 0. 05),and there were significant differences between A,B kits and C kits( P < 0. 05),but there was no significant difference between A and B kits( P > 0. 05). For weakly positive specimens detected by A and B kits,C kit was generally negative; for weakly positive specimens,agglutination strength ranking was A kit > B kit > C kit; for strongly positive specimens,agglutination strength ranking was B kit > A kit > C kit. Detection sensitivity ranking was B kit > A kit > C kit.Conclusion: It is recommended that clinical laboratories use B kit to test the blood transfusion compatibility of patients' specimens,which is supplemented by A kit and C kit for the comparison and exclusion.
引文
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[6]安勤发,胡志红,崔慧芹.微柱凝胶卡式法检测红细胞不规则抗体的效果[J].中国生物制品学杂志,2013,26(6):880-882.
[7]管政,张军,陈丽,郭博. 3种微柱凝胶抗人球蛋白卡在检测孕妇Ig G血型抗体效价中的应用比较[J].蚌埠医学院学报,2011,36(8):872-877.
[2]胡亚美,严纯.临床儿科诊疗关键[M].南宁:广西科学技术出版社,1999:29.
[3]陈维佳,王冬倩,童军.微柱凝胶免疫实验技术基础[M].2版.北京:科学出版社,2006:562-570.
[4]赵祥胜.柱凝聚技术检测孕妇血清Ig G抗体水平[J].临床输血与检验,2000,2(3):32-33.
[5]袁晓华,胡惠萍.国产微柱凝胶卡对血型鉴定的结果分析[J].国际检验医学杂志,2013,34(12):1585-1586.
[6]安勤发,胡志红,崔慧芹.微柱凝胶卡式法检测红细胞不规则抗体的效果[J].中国生物制品学杂志,2013,26(6):880-882.
[7]管政,张军,陈丽,郭博. 3种微柱凝胶抗人球蛋白卡在检测孕妇Ig G血型抗体效价中的应用比较[J].蚌埠医学院学报,2011,36(8):872-877.