摘要
目的建立葛根芩连丸(葛根、黄芩、黄连、炙甘草)UPLC指纹图谱,并测定葛根素、黄芩素、巴马汀、汉黄芩素、大豆苷、大豆苷元、药根碱的含有量。方法该药物70%甲醇提取液的分析采用Waters ACQUITY UPLC~ BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm);甲醇-0.1%冰醋酸为流动相,梯度洗脱;体积流量0.3 m L/min;检测波长260 nm;柱温30℃。结果 10批样品指纹图谱中有25个共有峰,相似度大于0.98。7种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率98.99%~103.6%,RSD 1.13%~2.03%。结论该方法简便可靠,重复性好,可用于葛根芩连丸的质量控制。
AIM To establish the UPLC fingerprints of Gegen Qinlian Pills( Puerariae lobatae Radix,Scutellariae Radix,Coptidis Rhizoma and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle) and to determine the contents of puerarin,baicalein,palmatine,wogonin,daidzin,daidzein and jateorhizine. METHODS The analysis of 70% methanol extract of this drug was performed on a 30 ℃ thermostatic Waters ACQUITY UPLC~ BEH C_(18) column,with the mobile phase comprising of methanol-0. 1% glacial acetic acid flowing at 0. 3 m L/min in a gradient manner,and the detection wavelength was set at 260 nm. RESULTS There were twenty-five common peaks in the fingerprints of ten batches of samples with the similarities of more than 0. 98. Seven constituents showed good linear relationships within their own ranges( r > 0. 999 0),whose average recoveries were 98. 99%-103. 6% with the RSDs of 1. 13%-2. 03%. CONCLUSION This simple,reliable and reproducible method can be used for the quality control of Gegen Qinlian Pills.
引文
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