商洛桔梗根不同部位桔梗皂苷D动态研究
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摘要
采用高效液相色谱法(HPLC),分析商洛种植的2年生桔梗根不同部位(须根、木质部和韧皮部)及不同月份(5—11月)中桔梗皂苷D的含量变化,以探究主要次生代谢产物在桔梗根中积累量的变化。结果显示,在5—11月期间,桔梗皂苷D含量在须根和木质部中稳步增加,二者分别在9,11月份达到最大值,而韧皮部中则变化不大,10月达到最大值;不同部位桔梗皂苷D平均含量大小为须根(0.410%)>韧皮部(0.182%)>木质部(0.039%)。建议商洛地区所种植桔梗采收期在10—11月,采收时须根要充分收集。研究结果可为确定商洛桔梗采收期以及为桔梗质量控制及规范化种植提供借鉴。
High performance liquid chromatography(HPLC)was utilized to analyze the variations of Platycodin D content in different parts(hair roots, xylems and phloems)in Platycodon grandiflorum(Jacq.)A. DC. radix, which were collected from May to November from Shangluo area, and the study was aimed to explore the variations of main secondary metabolites in radix. The results showed that the contents of Platycodin D increased stably in the radix from May to November in hair roots and xylems, and the peaks were reached in September and November, respectively. Platycodin D changed slightly in those months in phloems, and October had the highest content. Furthormore, the contents of Platycodin D were as follows:hair roots(0.410%)>xylems(0.182%)>xylems(0.039%).Therefore, the harvest time should be between October and November in Shangluo, and hair roots also should be collected completely.The study results will provide references for harvest time and standardization production in Platycodon grandiflorum in Shangluo.
High performance liquid chromatography(HPLC)was utilized to analyze the variations of Platycodin D content in different parts(hair roots, xylems and phloems)in Platycodon grandiflorum(Jacq.)A. DC. radix, which were collected from May to November from Shangluo area, and the study was aimed to explore the variations of main secondary metabolites in radix. The results showed that the contents of Platycodin D increased stably in the radix from May to November in hair roots and xylems, and the peaks were reached in September and November, respectively. Platycodin D changed slightly in those months in phloems, and October had the highest content. Furthormore, the contents of Platycodin D were as follows:hair roots(0.410%)>xylems(0.182%)>xylems(0.039%).Therefore, the harvest time should be between October and November in Shangluo, and hair roots also should be collected completely.The study results will provide references for harvest time and standardization production in Platycodon grandiflorum in Shangluo.
引文
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[2]张晓虎.秦岭“五大商药”施肥技术研究综述[J].陕西农业科学,2010,56(4):94-97.
[3]刘群,李伟,郑毅男,等.桔梗中三萜皂苷类成分及药理活性研究进展[J].吉林农业大学学报,2013,35(2):221-228.
[4]左军,尹柏坤,胡晓阳.桔梗化学成分及现代药理研究进展[J].辽宁中医药大学学报,2019,21(1):113-116.
[5] LEE J Y,YOON J W,KIM C T.Antioxidant activity of phenylpropanoid esters isolated and identified from Platycodon grandiflorum A. DC.[J]. Phytochemistry,2004,65(22):3033-3039.
[6]师绘敏,刘彦楠.桔梗叶多糖的提取工艺[J].天津农业科学,2016,22(7):37-40.
[7]董增,曹稳根,段红,等.桔梗多糖提取、分离纯化以及生物活性研究[J].基因组学与应用生物学,2018,37(8):3534-3539.
[8]侯巍,李国清,赵稷,等.纳米硒桔梗多糖复合物的降糖作用研究[J].微量元素与健康研究,2019,36(3):1-4.
[9]王新军,陈明彬,邓寒霜.基于生境条件的商洛桔梗道地性解析[J].商洛学院学报,2014,28(4):8-11,53.
[10]王新军,陈明彬,吴珍.商洛桔梗基于GIS的适宜气候区划及分区评价[J].陕西农业科学,2014,60(8):55-58.
[11]李堆淑,朱广啟.外源水杨酸对桔梗幼苗铅胁迫的缓解效应[J].山西农业科学,2016,44(8):1165-1168.
[12]申茹,徐英辉,莫颖华,等.山东省不同区域桔梗药材中桔梗皂苷D的测定[J].现代中药研究与实践,2018,32(4):11-13.
[13]赵姣姣,杨其长,刘文科.氮水平和形态对水培桔梗生长及氮磷吸收的影响[J].华北农学报,2013,28(6):220-225.
[14]陈明明,张红瑞,孟肖,等.不同播期对河南产桔梗根品质的影响[J].河南农业科学,2016,45(12):132-134,148.
[15]李珅,张红瑞,彭涛,等.产地和生长年限对桔梗品质的影响[J].河南农业科学,2018,47(3):112-115.