原油降解菌群对石油烃的生物演化过程研究
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摘要
本研究从大港油田受原油污染的土壤中筛得6株原油降解菌,通过生理生化特征实验、16S rDNA分子生物学鉴定和气相色谱(GC-FID)分析,对石油烃降解菌群的生物演化规律进行研究.结果表明,筛得的6株主要为假单胞菌和芽孢杆菌,芽孢杆菌与假单胞菌均为原油降解的优势菌种.将这6株菌按照相同体积比配制成YJ01混合菌处理含油海水,降解7 d后,混合菌群对中链、长链烃的降解效果较好;混合菌对奇碳数烃的降解优势更明显.
In this study six crude oil degrading bacteria were screened from crude oil contaminated soil in Dagang Oilfield.Through physiology biochemistry experiment,molecular biological techniques of 16 S rDNA and GC-FIR,the biological evolution of petroleum-degrading bacteria was studied.The results showed that the six strains were mainly Pseudomonas and Bacillus.Bacillus and Pseudomonas are the dominant strains of crude oil degradation.According to the same volume ratio the six strains were formulated into YJ01 mixed bacteria to treat sea water contaminated with crude oil.After degraded for 7 days the mixed bacteria had a good degradation effect on medium-chain and long-chain hydrocarbons.The degradation capability of the mixed bacteria to the odd carbon alkanes was higher.
In this study six crude oil degrading bacteria were screened from crude oil contaminated soil in Dagang Oilfield.Through physiology biochemistry experiment,molecular biological techniques of 16 S rDNA and GC-FIR,the biological evolution of petroleum-degrading bacteria was studied.The results showed that the six strains were mainly Pseudomonas and Bacillus.Bacillus and Pseudomonas are the dominant strains of crude oil degradation.According to the same volume ratio the six strains were formulated into YJ01 mixed bacteria to treat sea water contaminated with crude oil.After degraded for 7 days the mixed bacteria had a good degradation effect on medium-chain and long-chain hydrocarbons.The degradation capability of the mixed bacteria to the odd carbon alkanes was higher.
引文
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[27]王冬梅,陈丽华,雒晓芳,等.鼠李糖脂与菌剂对原油污染土壤的联合修复[J].环境工程学报,2014,8(11):5003-5009.
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[2]Li P,Cai Q H,Lin W Y,et al.Offshore oil spill response practices and emerging challenges[J].Marine Pollution Bulletin,2016,110(1):6-27.
[3]Xing Q G,Li L,Lou M J,et al.Observation of oil spills through landsat thermal infrared imagery:a case of deepwater horizon[J].Aquatic Procedia,2015(3):151-156.
[4]Xing Q G,Meng R L,Lou M J,et al.Remote sensing o ships and offshore oil platforms and mapping the marine oi spill risk source in the bohai sea[J].Aquatic Procedia,2015(3):127-132.
[5]刘玉华,王慧,胡晓珂.不动杆菌属(Acinetobacter)细菌降解石油烃的研究进展[J].微生物学通报,2016,43(7):1579-1589.
[6]高悦,王呈玉,李洪敏,等.原油降解菌的筛选及其降解特性[J].吉林农业大学学报,2015,37(1):77-82.
[7]邱清华,哈尼帕,邓绍云,等.石油降解混合菌剂的筛选及降解条件研究[J].中国农学通报,2013,29(3):184-189.
[8]郝婧,高磊,吴春旭,等.石油降解菌Pseudomonas stutzeri TH-31的分离与降解条件优化[J].环境工程学报,2015,9(4):1771-1777.
[9]Wang J,Sandoval K,Ding Y,et al.Biodegradation of dispersed Macondo crude oil by indigenous Gulf of Mexico microbial communities[J].Science of the Total Environment,2016,557/558:453-468.
[10]Varjani S J,Rana D P,Jain A K,et al.Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites o Gujarat,India[J].International Biodeterioration&Biodegradation,2015,103:116-124.
[11]Prince R C,Nash G W,Hill S J.The biodegradation of crude oil in the deep ocean[J].Marine Pollution Bulletin,2016,111(1/2):354-357.
[12]Kristensen M,Johnsen A R,Christensen J H.Marine biodegradation of crude oil in temperate and Arctic water samples[J].Journal of Hazardous Materials,2015,300:75-83.
[13]余薇,王海云,梁运祥.水环境石油烃降解菌的分离筛选及其降解特性研究[J].环境科学与技术,2013,36(10):101-106.
[14]马朝阳,齐树亭,李斯,等.两株海洋原油降解菌的筛选鉴定及降解特性的初步研究[J].河北工业大学学报,2016,45(6):82-90.
[15]陈丽华,孙万虹,李海玲,等.石油降解菌对石油烃中不同组分的降解及演化特征研究[J].环境科学学报,2016,36(1):124-133.
[16]马静.多环芳烃降解菌的筛选、降解机理及降解性能研究[D].大连:大连理工大学,2013.
[17]何丽媛,党志,唐霞,等.混合菌对原油的降解及其降解性能的研究[J].环境科学学报,2010,30(6):1220-1227.
[18]周楚莹,吴秉奇,刘淑杰,等.近海柴油降解菌群的构建及其对柴油的降解特性[J].微生物学通报,2015,42(12):2308-2320.
[19]汤瑶.基于PCR-DGGE技术的原油降解菌群落结构分析[D].天津:天津理工大学,2016.
[20]赵姣,屈撑囤,鱼涛,等.高效原油降解菌的分离鉴定及降解特性分析[J].油田化学,2017,34(3):532-537.
[21]国家发展和改革委员会.SY/T 5119-2008中国标准书号[S].北京:石油工业出版社,2008.
[22]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 21247-2007中国标准书号[S].北京:中国标准出版社,2007.
[23]国家发展和改革委员会.SY/T 5779-2008中国标准书号[S].北京:石油工业出版社,2008.
[24]卢进才,魏仙样,魏建设,等.内蒙古西部额济纳旗雅干地区二叠系埋汗哈达组烃源岩的特征[J].地质通报,2010,29(2-3):341-345.
[25]杨雪娜,马启敏,刘新军.胶州湾滩涂养殖区沉积物脂肪烃的特征参数[J].环境化学,2015,34(5):932-938.
[26]沈佳恒,肖国桥,王治祥,等.秦安中新世风尘堆积正构烷烃的分布特征及古环境意义[J].中国科学:地球科学,2017,47(4):485-493.
[27]王冬梅,陈丽华,雒晓芳,等.鼠李糖脂与菌剂对原油污染土壤的联合修复[J].环境工程学报,2014,8(11):5003-5009.
[28]何延龙,张凡,柴陆军,等.注采水样微生物群落与高凝原油的相互作用[J].中国石油大学学报:自然科学版,2015,39(4):131-139.