HPLC测定葶苈子炮制前后5个成分的含量变化
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摘要
目的:建立葶苈子中5个指标成分的HPLC含量测定方法,研究炮制前后葶苈子中5个成分的含量变化规律。方法:采用HPLC同时测定槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷(QGG),芥子酸,槲皮素-3-O-β-D-吡喃葡萄糖苷(QG),异鼠李素-3-O-β-D-吡喃葡萄糖苷(IG)和1,2-O-二-芥子酰基-β-D-吡喃葡萄糖苷(SG)共5个成分的含量,分析炮制前后的含量变化规律及其原因。色谱条件为Waters Symmetry~ C_(18)色谱柱(4. 6 mm×250 mm,5μm),流动相乙腈(A)-1%乙酸水溶液(B)梯度洗脱(0～5 min,5%～10%A; 5～15 min,10%～13%A; 15～23 min,13%～20%A; 23～43 min,20%～25%A; 43～46 min,25%A; 46～55 min,25%～40%A; 55～60 min,40%A),流速1 m L·min~(-1),检测波长265 nm,进样量10μL,柱温30℃。结果:上述5个成分炮制前的质量分数分别为0. 114 3%,0. 041 6%,0. 036 2%,0. 022 6%,0. 097 6%;炮制后的质量分数分别为0. 107 4%,0. 011 3%,0. 034 2%,0. 021 9%,0. 058 9%; 5个成分的质量分数分别下降了6. 04%,72. 84%,5. 52%,3. 10%,39. 65%。结论:葶苈子中所含的这5个成分含量经炮制后均有不同程度的降低,其中苯丙素类成分炮制后含量明显下降,而黄酮苷类成分的含量变化则不明显。
Objective: HPLC for the determination of five components in Descurainiae Semen was established to investigate the change rule of contents of five components in the herb before and after being processed. Method: The contents of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside( QGG), sinapic acid,quercetin-3-O-β-D-glucopyranoside( QG),isorhamnetin-3-O-β-D-glucopyranoside( IG) and 1,2-di-O-sinapoyl-β-D-glucopyranose( SG) was determined simultaneously by HPLC, the change rule of contents of these components before and after processing and its reasons were analyzed. Waters Symmetry~ C_(18) column( 4. 6 mm ×250 mm,5 μm) was employed,and the mobile phase was acetonitrile( A)-1% acetic acid aqueous solution( B) for gradient elution( 0-5 min,5%-10% A; 5-15 min,10%-13% A; 15-23 min,13%-20% A; 23-43 min,20%-25% A; 43-46 min,25% A; 46-55 min,25%-40% A; 55-60 min,40% A). The flow rate was1 m L·min~(-1). The detection wavelength was set at 265 nm,the injection volume was 10 μL,and the column temperature was 30 ℃. Result: Contents of the above five components before processing were 0. 114 3%,0. 041 6%,0. 036 2%,0. 022 6% and 0. 097 6%; after processing,the contents of these five components turn into 0. 107 4%,0. 011 3%,0. 034 2%,0. 021 9% and 0. 058 9%; among them,the contents of these five components decreased by 6. 04%,72. 84%,5. 52%,3. 10% and 39. 65%, respectively. Conclusion: The contents of these five components in Descurainiae Semen is reduced to varying degrees after processing. The contents of phenylpropanoids decrease significantly, while the contents of flavonoid glycosides do not change significantly.
Objective: HPLC for the determination of five components in Descurainiae Semen was established to investigate the change rule of contents of five components in the herb before and after being processed. Method: The contents of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside( QGG), sinapic acid,quercetin-3-O-β-D-glucopyranoside( QG),isorhamnetin-3-O-β-D-glucopyranoside( IG) and 1,2-di-O-sinapoyl-β-D-glucopyranose( SG) was determined simultaneously by HPLC, the change rule of contents of these components before and after processing and its reasons were analyzed. Waters Symmetry~ C_(18) column( 4. 6 mm ×250 mm,5 μm) was employed,and the mobile phase was acetonitrile( A)-1% acetic acid aqueous solution( B) for gradient elution( 0-5 min,5%-10% A; 5-15 min,10%-13% A; 15-23 min,13%-20% A; 23-43 min,20%-25% A; 43-46 min,25% A; 46-55 min,25%-40% A; 55-60 min,40% A). The flow rate was1 m L·min~(-1). The detection wavelength was set at 265 nm,the injection volume was 10 μL,and the column temperature was 30 ℃. Result: Contents of the above five components before processing were 0. 114 3%,0. 041 6%,0. 036 2%,0. 022 6% and 0. 097 6%; after processing,the contents of these five components turn into 0. 107 4%,0. 011 3%,0. 034 2%,0. 021 9% and 0. 058 9%; among them,the contents of these five components decreased by 6. 04%,72. 84%,5. 52%,3. 10% and 39. 65%, respectively. Conclusion: The contents of these five components in Descurainiae Semen is reduced to varying degrees after processing. The contents of phenylpropanoids decrease significantly, while the contents of flavonoid glycosides do not change significantly.
引文
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[19]马梅芳,吕文海.高效液相色谱法测定南葶苈子饮片中芥子碱硫氰酸盐含量[J].中国药业,2008,17(5):14-15.
[20]马梅芳,吕文海. HPLC法同时测定南葶苈子饮片中槲皮素、山奈酚及异鼠李素的含量[J].中国药事,2009,23(1):65-67.
[21]李红伟,司金光,石延榜,等.采用GC-MS法分析炮制对南葶苈子脂肪油成分的影响[J].中国新药杂志,2015,24(24):2868-2872.
[22]吴伟,金鸣,李金荣,等.葶苈子黄酮对血小板激活因子的拮抗作用[J].中草药,2006,37(10):1539-1541.
[23] SUN K,LI X,LI W,et al. Two new lactones and one new aryl-8-oxa-bicyclo[3, 2, 1] oct-3-en-2-one from Descurainia sophia[J]. Chem Pharm Bull(Tokyo),2004,52(12):1483-1486.
[24]孙凯,李铣.南葶苈子的化学成分[J].沈阳药科大学学报,2003,20(6):419-421.
[25]马丛丛,许继取,韩领,等.芥子酸及其生物活性研究进展[J].中国油脂,2016,41(5):75-79.
[26]周喜丹.葶苈子化学成分及饮片鉴别研究[D].北京:中国中医科学院,2015.
[27] Koski A,Pekkarinen S,Hopia A,et al. Processing of rapeseed oil:effects on sinapic acid derivative content and oxidative stability[J]. Eur Food Res Technol,2003,217(2):110-114.
[28] Wakamatsu D,Morimura S,Rahaman M,et al. Isolation,identification,and structure of a potent alkyl-peroxyl radical scavenger in crude canola oil,canolol[J]. Biosci Biotechnol Biochem,2005,69(8):1568-1574.
[29] Spielmeyer A,Wagner A,Jahreis G. Influence of thermal treatment of rapeseed on the canolol content[J]. Food Chem,2009,112(4):944-948.
[30]李进冉,郭宝林,陈安家,等.炒南葶苈子饮片的质量标准研究[J].中国实验方剂学杂志,2010,16(8):35-37.
[2]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:333-334.
[3]高学敏.中药学[M].北京:中国中医药出版社,2007:375.
[4]周喜丹,唐力英,周国洪,等.南北葶苈子的最新研究进展[J].中国中药杂志,2014,39(24):4699-4708.
[5]李红伟,郑晓珂,弓建红,等.独行菜和播娘蒿化学成分及药理作用研究进展[J].药物评价研究,2013,36(3):235-240.
[6]赵海誉,范妙璇,石晋丽,等.北葶苈子化学成分研究[J].中草药,2010,41(1):14-18.
[7]王爱芹,王秀坤,李军林,等.南葶苈子化学成分的分离与结构鉴定[J].药学学报,2004,39(1):46-51.
[8]张国顺,白义萍,王小兰,等.葶苈子抗心衰有效组分筛选及其作用机制分析[J].中国实验方剂学杂志,2017,23(4):118-125.
[9]郭娟,陈长勋,沈云辉.葶苈子水提液对动物实验性心室重构的影响[J].中草药,2007,38(10):1519-1523.
[10]张晓丹,范春兰,余迎梅,等.葶苈子水提液对CHF大鼠利尿作用的影响[J].中国现代应用药学,2010,27(3):210-213.
[11]吴晓玲,杨裕忠,黄东亮.葶苈子水提物对狗左心室功能的作用[J].中药材,1998,21(5):243-245.
[12]杨云,赫金丽,孙亚萍,等.葶苈子化学拆分组分止咳祛痰平喘作用研究[J].世界科学技术—中医药现代化,2015,17(3):514-519.
[13]柏正平,郑兵,卜献春,等.复方葶苈子胶囊止咳祛痰作用的实验研究[J].湖南中医药导报,2000,6(5):41-42.
[14]马梅芳,李芳.葶苈子对昆明种小鼠移植H22肝癌移植瘤抑瘤作用的研究[J].中华中医药学刊,2014,32(2):385-386.
[15]马梅芳,李洁.葶苈子对S180荷瘤小鼠动物模型的影响[J].中华中医药学刊,2014,32(1):157-158.
[16]刘忠良.南葶苈子提取物调血脂作用的实验研究[J].药学实践杂志,2000,18(1):15-17.
[17]陆兔林,胡昌江.中药炮制学[M].北京:中国医药科技出版社,2014:36-39.
[18]龚千锋.中药炮制学[M].北京:中国中医药出版社,2003:22-25.
[19]马梅芳,吕文海.高效液相色谱法测定南葶苈子饮片中芥子碱硫氰酸盐含量[J].中国药业,2008,17(5):14-15.
[20]马梅芳,吕文海. HPLC法同时测定南葶苈子饮片中槲皮素、山奈酚及异鼠李素的含量[J].中国药事,2009,23(1):65-67.
[21]李红伟,司金光,石延榜,等.采用GC-MS法分析炮制对南葶苈子脂肪油成分的影响[J].中国新药杂志,2015,24(24):2868-2872.
[22]吴伟,金鸣,李金荣,等.葶苈子黄酮对血小板激活因子的拮抗作用[J].中草药,2006,37(10):1539-1541.
[23] SUN K,LI X,LI W,et al. Two new lactones and one new aryl-8-oxa-bicyclo[3, 2, 1] oct-3-en-2-one from Descurainia sophia[J]. Chem Pharm Bull(Tokyo),2004,52(12):1483-1486.
[24]孙凯,李铣.南葶苈子的化学成分[J].沈阳药科大学学报,2003,20(6):419-421.
[25]马丛丛,许继取,韩领,等.芥子酸及其生物活性研究进展[J].中国油脂,2016,41(5):75-79.
[26]周喜丹.葶苈子化学成分及饮片鉴别研究[D].北京:中国中医科学院,2015.
[27] Koski A,Pekkarinen S,Hopia A,et al. Processing of rapeseed oil:effects on sinapic acid derivative content and oxidative stability[J]. Eur Food Res Technol,2003,217(2):110-114.
[28] Wakamatsu D,Morimura S,Rahaman M,et al. Isolation,identification,and structure of a potent alkyl-peroxyl radical scavenger in crude canola oil,canolol[J]. Biosci Biotechnol Biochem,2005,69(8):1568-1574.
[29] Spielmeyer A,Wagner A,Jahreis G. Influence of thermal treatment of rapeseed on the canolol content[J]. Food Chem,2009,112(4):944-948.
[30]李进冉,郭宝林,陈安家,等.炒南葶苈子饮片的质量标准研究[J].中国实验方剂学杂志,2010,16(8):35-37.