不同时间睡眠干扰致小鼠体力疲劳模型的建立
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摘要
目的研究不同时间睡眠干扰(sleep interruption,SI)后所致小鼠体力疲劳,为缓解体力疲劳产品研发提供稳定可靠的动物模型。方法使用滚筒睡眠干扰仪对小鼠进行不同时间(5,10,15 d)睡眠干扰后,对各组动物进行转轮实验和负重游泳实验,并测定小鼠肝糖原和血清尿素氮的含量。结果转轮实验中,睡眠干扰5 d、10 d、15 d组与对照组比较时间有减少的趋势,但差异无显著性(P> 0. 05)。睡眠干扰10 d、15 d组与空白对照组比较,负重游泳力竭时间明显缩短(P<0. 05,P <0. 01),下沉次数和下沉总时间显著减少(P <0. 01,P <0. 01)。干扰5 d、10 d、15 d组与对照组比较,肝糖原含量明显降低(P <0. 05);干扰10 d、15 d组与对照组比较,血清尿素氮含量显著升高(P <0. 05)。结论滚筒法睡眠干扰(滚动参数3 r/min,每转1 min,间隔1 min) 10,15 d均可引起小鼠的体力疲劳行为学表现。
Objective To study the physical fatigue behavioral manifestation of mice after sleep interruption( SI)at different timepoints,to provide a stable and reliable animal model for the research and development of general health products. Methods Mice were placed into a rotating drum sleep interferometer for different periods of time( 5,10,and 15 days). Then,the rotating wheel and loaded swimming tests were used to assess the behavioral activities of mice after SI.The hepatic glycogen and serum urea nitrogen levels were used to assess the biochemical index after SI. Results In the rotating wheel exhausted test,the exhausted time of SI in the 5 d,10 d and 15 d groups were decreased compared with the control group,but with a non-significant difference( P> 0. 05). In the loaded swimming test,compared with the control group,the mice after SI for 10 and 15 days showed significantly decreased exhaustive time( P< 0. 05,P < 0. 01),sink frequency and total sink time( P < 0. 01,P < 0. 01). In addition,our study demonstrated that the content of hepatic glycogen was significantly reduced in mice after SI for 5,10,and 15 days( P < 0. 05) compared with the control group,and that the serum urea nitrogen level of mice after SI for 10 and 15 days was increased( P < 0. 05). Conclusions A mouse model of physical fatigue behavior can be established by sleep interruption using a sleep interruption apparatus set at rotation speed,20 s per revolution; and rotation frequency,rotating 1 min after 1 min pause for 10 days and 15 days.
Objective To study the physical fatigue behavioral manifestation of mice after sleep interruption( SI)at different timepoints,to provide a stable and reliable animal model for the research and development of general health products. Methods Mice were placed into a rotating drum sleep interferometer for different periods of time( 5,10,and 15 days). Then,the rotating wheel and loaded swimming tests were used to assess the behavioral activities of mice after SI.The hepatic glycogen and serum urea nitrogen levels were used to assess the biochemical index after SI. Results In the rotating wheel exhausted test,the exhausted time of SI in the 5 d,10 d and 15 d groups were decreased compared with the control group,but with a non-significant difference( P> 0. 05). In the loaded swimming test,compared with the control group,the mice after SI for 10 and 15 days showed significantly decreased exhaustive time( P< 0. 05,P < 0. 01),sink frequency and total sink time( P < 0. 01,P < 0. 01). In addition,our study demonstrated that the content of hepatic glycogen was significantly reduced in mice after SI for 5,10,and 15 days( P < 0. 05) compared with the control group,and that the serum urea nitrogen level of mice after SI for 10 and 15 days was increased( P < 0. 05). Conclusions A mouse model of physical fatigue behavior can be established by sleep interruption using a sleep interruption apparatus set at rotation speed,20 s per revolution; and rotation frequency,rotating 1 min after 1 min pause for 10 days and 15 days.
引文
[1] Gandevia SC. Some central and peripheral factors affecting human motoneuronal output in neuromuscular fatigue[J]. Sports Med,1992,13(2):93-98.
[2] Hagberg M. Muscular endurance and surface electromyogram in isometric and dynamic exercise[J]. J Appl Physiol Respir Environ Exerc Physiol,1981,51(1):1-7.
[3] Hawley JA,Reilly T. Fatigue revisited[J]. J Sports Sci,1997,15(3):245-246.
[4]杨菊贤,杜勤.慢性疲劳与失眠是亚健康的重要表现[J].上海预防医学,2003,15(1):48-49.
[5] Claghorn GC,Fonseca IA,Thompson Z,et al. Serotoninmediated central fatigue underlies increased endurance capacity in mice from lines selectively bred for high voluntary wheel running[J]. Physiol Behav,2016,161:145-154.
[6] Ikeuchi M,Koyama T,Takahashi J,et al. Effects of astaxanthin supplementation on exercise-induced fatigue in mice[J]. Biol Pharm Bull,2006,29(10):2106-2110.
[7]王天芳,陈易新,季绍良,等.慢性束缚致慢性疲劳动物模型的研制及其行为学观察[J].中国中医基础医学杂志,1999,5(5):25-29.
[8] Verret L,Fort P,Gervasoni D,et al. Localization of the neurons active during paradoxical(REM)sleep and projecting to the locus coeruleus noradrenergic neurons in the rat[J]. J Comp Neurol,2010,495(5):573-586.
[9]卜兰兰,石哲,武宏伟,等.达玛烷苷元对睡眠干扰所致小鼠学习记忆障碍的改善作用[J].中国比较医学杂志,2014,24(10):48-53.
[10] Lu C,Shi Z,Dong L,et al. Exploring the effect of ginsenoside Rh1 in a sleep deprivation-induced mouse memory impairment model[J]. Phytother Res,2017,31(5):763-770.
[11]高江晖,卜兰兰,石哲,等.不同时间睡眠干扰所致小鼠的类抑郁样行为[J].中国实验动物学报,2011,19(5):405-409.
[12]张北月,石晋丽,郑志全,等. DS-1226对慢性睡眠干扰所致小鼠抑郁行为的改善作用[J].中国实验动物学报,2017,25(1):85-89.
[13]苏成虎,陈晓农,杨新波,等.水芹提取物抗运动性疲劳作用及初步机制分析[J].解放军药学学报,2011,27(2):103-106.
[14]谢磊,李由,刘新民,等.小鼠游泳耐力实验系统的建立与红景天抗疲劳作用的验证[J].中国比较医学杂志,2016,26(5):71-76.
[15] Takeda K,Takemasa T. Expression of ammonia transporters Rhbg and Rhcg in mouse skeletal muscle and the effect of 6-week training on these proteins[J]. Physiol Rep, 2015, 3(10):e12596.
[16]何来英,严卫星,楼密密,等.抗疲劳试验中肝糖原测定的意义探讨[J].中国食品卫生杂志,1998,10(6):1-5.
[17] Dolny DG,Lemon PW. Effect of ambient temperature on protein breakdown during prolonged exercise[J]. J Appl Physiol(1985),1988,64(2):550-555.
[2] Hagberg M. Muscular endurance and surface electromyogram in isometric and dynamic exercise[J]. J Appl Physiol Respir Environ Exerc Physiol,1981,51(1):1-7.
[3] Hawley JA,Reilly T. Fatigue revisited[J]. J Sports Sci,1997,15(3):245-246.
[4]杨菊贤,杜勤.慢性疲劳与失眠是亚健康的重要表现[J].上海预防医学,2003,15(1):48-49.
[5] Claghorn GC,Fonseca IA,Thompson Z,et al. Serotoninmediated central fatigue underlies increased endurance capacity in mice from lines selectively bred for high voluntary wheel running[J]. Physiol Behav,2016,161:145-154.
[6] Ikeuchi M,Koyama T,Takahashi J,et al. Effects of astaxanthin supplementation on exercise-induced fatigue in mice[J]. Biol Pharm Bull,2006,29(10):2106-2110.
[7]王天芳,陈易新,季绍良,等.慢性束缚致慢性疲劳动物模型的研制及其行为学观察[J].中国中医基础医学杂志,1999,5(5):25-29.
[8] Verret L,Fort P,Gervasoni D,et al. Localization of the neurons active during paradoxical(REM)sleep and projecting to the locus coeruleus noradrenergic neurons in the rat[J]. J Comp Neurol,2010,495(5):573-586.
[9]卜兰兰,石哲,武宏伟,等.达玛烷苷元对睡眠干扰所致小鼠学习记忆障碍的改善作用[J].中国比较医学杂志,2014,24(10):48-53.
[10] Lu C,Shi Z,Dong L,et al. Exploring the effect of ginsenoside Rh1 in a sleep deprivation-induced mouse memory impairment model[J]. Phytother Res,2017,31(5):763-770.
[11]高江晖,卜兰兰,石哲,等.不同时间睡眠干扰所致小鼠的类抑郁样行为[J].中国实验动物学报,2011,19(5):405-409.
[12]张北月,石晋丽,郑志全,等. DS-1226对慢性睡眠干扰所致小鼠抑郁行为的改善作用[J].中国实验动物学报,2017,25(1):85-89.
[13]苏成虎,陈晓农,杨新波,等.水芹提取物抗运动性疲劳作用及初步机制分析[J].解放军药学学报,2011,27(2):103-106.
[14]谢磊,李由,刘新民,等.小鼠游泳耐力实验系统的建立与红景天抗疲劳作用的验证[J].中国比较医学杂志,2016,26(5):71-76.
[15] Takeda K,Takemasa T. Expression of ammonia transporters Rhbg and Rhcg in mouse skeletal muscle and the effect of 6-week training on these proteins[J]. Physiol Rep, 2015, 3(10):e12596.
[16]何来英,严卫星,楼密密,等.抗疲劳试验中肝糖原测定的意义探讨[J].中国食品卫生杂志,1998,10(6):1-5.
[17] Dolny DG,Lemon PW. Effect of ambient temperature on protein breakdown during prolonged exercise[J]. J Appl Physiol(1985),1988,64(2):550-555.