云南德宏水牛和杂交牛(摩拉水牛×德宏水牛)血清中两种同工酶的多态性研究
|
摘要
本试验采用聚丙烯酰胺凝胶电泳(PAGE)对云南德宏水牛和杂交牛(摩拉水牛×德宏水牛)血清中乳酸脱氢酶(LDH)和过氧化物酶(POD)两种同工酶多态性进行研究,并采用GelDocTM.XR凝胶成像系统扫描仪对乳酸脱氢酶进行定量。结果表明两种牛血清中的两种同工酶均出现多态性,且德宏水牛血清同工酶多态性少于杂交牛。德宏水牛LDH1、LDH2、LDH3、LDH4、LDH5相对百分含量依次为47.25±1.62%、25.41±0.75%、14.07±0.49%、10.55±0.97%、5.98±0.45%;杂交牛依次为49.27±0.85%、26.26±0.55%、12.48±0.40%、8.89±0.52%、6.35±0.27%。对LDH相对百分含量进行方差分析的结果显示,两种牛之间只有LDH3差异显著(P<0.05),其余差异均不显著(P>0.05)。该结果为进一步研究血清同工酶的多态性与生产性能之间的关系提供了理论依据。
The genetic characteristics of serum lactate dehydrogenase(LDH) and peroxidase(POD) isozymes of Dehong buffalo and Hybrid Buffalo(Moura Buffalo × Dehong Buffalo)(referred to as Moben Hybridization) in Yunnan Province were detected with polyacrylamide gel electrophoresis(PAGE). Serum LDH isozyme was scanned bying gel imaging system. The result showed that the two isozymes were Polymorphism. The polymorphism of Dehong buffalo was less than that of Dehong Hybrid buffalo. The relative contents of LDH1, LDH2, LDH3, LDH4 and LDH5 in Dehong buffalo serum were 47.25 ± 1.62%, 25.41 ± 0.75%, 14.07 ± 0.49%, 10.55 ± 0.97% and 5.98 ±0.45% respectively, while they were 49.27 ± 0.85 %, 26.26 ± 0.55%, 12.48 ± 0.40%, 8.89 ± 0.52% and 6.35 ± 0.27%respectively in Hybrid buffalo. Variance analysis showed that LDH3 in the two breeds had significant differences(P<0.05), but there were no significant differences among other types of LDH in Dehong buffalo and Hybrid buffalo(P>0.05). This result will provide a theoretical basis for further study of the relationship between serum isoenzyme polymorphism and production performance.
The genetic characteristics of serum lactate dehydrogenase(LDH) and peroxidase(POD) isozymes of Dehong buffalo and Hybrid Buffalo(Moura Buffalo × Dehong Buffalo)(referred to as Moben Hybridization) in Yunnan Province were detected with polyacrylamide gel electrophoresis(PAGE). Serum LDH isozyme was scanned bying gel imaging system. The result showed that the two isozymes were Polymorphism. The polymorphism of Dehong buffalo was less than that of Dehong Hybrid buffalo. The relative contents of LDH1, LDH2, LDH3, LDH4 and LDH5 in Dehong buffalo serum were 47.25 ± 1.62%, 25.41 ± 0.75%, 14.07 ± 0.49%, 10.55 ± 0.97% and 5.98 ±0.45% respectively, while they were 49.27 ± 0.85 %, 26.26 ± 0.55%, 12.48 ± 0.40%, 8.89 ± 0.52% and 6.35 ± 0.27%respectively in Hybrid buffalo. Variance analysis showed that LDH3 in the two breeds had significant differences(P<0.05), but there were no significant differences among other types of LDH in Dehong buffalo and Hybrid buffalo(P>0.05). This result will provide a theoretical basis for further study of the relationship between serum isoenzyme polymorphism and production performance.
引文
[1]杨子姗,谭琳,姜开梅,等.云南省德宏州奶水牛业可持续发展前景分析[J].热带农业科学, 2012, 32(2):88-92.
[2]简保权,秦学,敏龚芳.世界水牛奶业发展现状和典型模式分析[J].世界农业, 2015, 3:115-118.
[3]吴明华.德宏州水牛奶开发利用及对策[J].中国乳业,2004,9:22-23.
[4]韩刚.我国水牛奶的营养价值[J].中国奶牛, 1995, 6:42-43.
[5]戴江河,石昭意,潘斌,等.水牛奶营养与功能研究进展[J].中国奶牛, 2017,6:10-13.
[6]李瑞生,梅国栋,马玉洁,等.德宏州杂交牛发展现状、存在问题及发展思路[J].草业与畜牧, 2014,3:34-38.
[7]牛红军,李杨.同工酶技术及其优化[J].生物学通报,2014,49(5):11.
[8]Nagy O, Cs, Tóthová, Seidel H, et al. The effect of respiratory diseasesonserumlactatedehydrogenaseanditsisoenzyme patterns in calves[J]. Polish Journal of Veterinary Sciences, 2013,16(2):211-218.
[9]KoukourakisMI,AlexandraG.Warburgeffect,Lactate DehydrogenaseandRadio/Chemo-therapyefficacy[J].International Journal of Radiation Biology, 2018:1-55.
[10]张晓红,张虎芳,王青.百草枯对金鱼血清过氧化物酶和酯酶表达的影响[J].癌变·畸变·突变, 2010, 22(5):391-393.
[11]陈晓虹,王林嵩,瞿文元.河南省小鲵科两种动物酯酶、过氧化物酶同工酶的比较[J].动物学杂志, 2000, 35:19-25.
[12]余瑞元,袁明秀,陈丽蓉,等.生物化学实验原理和方法[M].北京:北京大学出版社, 2005, 8:323-331.
[13]徐忠东,直塞呈.同工酶研究及其应用进展[J].安徽教育学院学报, 2000, 18:43-45.
[14]湛润生,杨珍,郭媛媛.山西黑猪不同器官组织乳酸脱氢酶同工酶活性分析[J].黑龙江畜牧兽医, 2017,(12上):128-131.
[15]邓艳美,叶绍辉.牛血清LDH同工酶遗传多样性及其在遗传育种中的应用[J].山东农业科学, 2006, 1:98-100.
[16]栾桂龙,韦莉莉,将艳明.水牛血清乳酸脱氢酶同工酶与产奶量的关系[J].广西科学, 1996, 3(3):41-44.
[17]Hoover K, Kishida K T, Digiorgio L A, et al. Inhibition of Baculoviral Disease by Plant-Mediated Peroxidase Activity and Free Radical Generation[J]. Journal of Chemical Ecology, 1998,24(12):1949-2001.
[18]Wu Y, Yang Z, How J, et al. Overexpression of a peroxidase gene(AtPrx64)of Arabidopsis thaliana in tobacco improves plant’s tolerance to aluminum stress[J]. Plant Molecular Biology, 2017.
[19]Cui Y, Liu J, Liu Y. Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean(Phaseolus vulgaris L)infected with Fusarium oxysporum f. sp.Phaseoli[J]. Molecular Breeding, 2014, 34(3):855-870.
[20]周卫生.早熟·晚熟猫尾草过氧化物酶同工酶及抗寒性分析[J].安徽农业科学, 2010, 38(32):18011-18013.
[2]简保权,秦学,敏龚芳.世界水牛奶业发展现状和典型模式分析[J].世界农业, 2015, 3:115-118.
[3]吴明华.德宏州水牛奶开发利用及对策[J].中国乳业,2004,9:22-23.
[4]韩刚.我国水牛奶的营养价值[J].中国奶牛, 1995, 6:42-43.
[5]戴江河,石昭意,潘斌,等.水牛奶营养与功能研究进展[J].中国奶牛, 2017,6:10-13.
[6]李瑞生,梅国栋,马玉洁,等.德宏州杂交牛发展现状、存在问题及发展思路[J].草业与畜牧, 2014,3:34-38.
[7]牛红军,李杨.同工酶技术及其优化[J].生物学通报,2014,49(5):11.
[8]Nagy O, Cs, Tóthová, Seidel H, et al. The effect of respiratory diseasesonserumlactatedehydrogenaseanditsisoenzyme patterns in calves[J]. Polish Journal of Veterinary Sciences, 2013,16(2):211-218.
[9]KoukourakisMI,AlexandraG.Warburgeffect,Lactate DehydrogenaseandRadio/Chemo-therapyefficacy[J].International Journal of Radiation Biology, 2018:1-55.
[10]张晓红,张虎芳,王青.百草枯对金鱼血清过氧化物酶和酯酶表达的影响[J].癌变·畸变·突变, 2010, 22(5):391-393.
[11]陈晓虹,王林嵩,瞿文元.河南省小鲵科两种动物酯酶、过氧化物酶同工酶的比较[J].动物学杂志, 2000, 35:19-25.
[12]余瑞元,袁明秀,陈丽蓉,等.生物化学实验原理和方法[M].北京:北京大学出版社, 2005, 8:323-331.
[13]徐忠东,直塞呈.同工酶研究及其应用进展[J].安徽教育学院学报, 2000, 18:43-45.
[14]湛润生,杨珍,郭媛媛.山西黑猪不同器官组织乳酸脱氢酶同工酶活性分析[J].黑龙江畜牧兽医, 2017,(12上):128-131.
[15]邓艳美,叶绍辉.牛血清LDH同工酶遗传多样性及其在遗传育种中的应用[J].山东农业科学, 2006, 1:98-100.
[16]栾桂龙,韦莉莉,将艳明.水牛血清乳酸脱氢酶同工酶与产奶量的关系[J].广西科学, 1996, 3(3):41-44.
[17]Hoover K, Kishida K T, Digiorgio L A, et al. Inhibition of Baculoviral Disease by Plant-Mediated Peroxidase Activity and Free Radical Generation[J]. Journal of Chemical Ecology, 1998,24(12):1949-2001.
[18]Wu Y, Yang Z, How J, et al. Overexpression of a peroxidase gene(AtPrx64)of Arabidopsis thaliana in tobacco improves plant’s tolerance to aluminum stress[J]. Plant Molecular Biology, 2017.
[19]Cui Y, Liu J, Liu Y. Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean(Phaseolus vulgaris L)infected with Fusarium oxysporum f. sp.Phaseoli[J]. Molecular Breeding, 2014, 34(3):855-870.
[20]周卫生.早熟·晚熟猫尾草过氧化物酶同工酶及抗寒性分析[J].安徽农业科学, 2010, 38(32):18011-18013.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |