同工酶检测方法及其研究进展
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摘要
同工酶是能够催化相同的生化反应,而分子结构和理化性质各异的一类酶,其几乎存在于所有生物的组织中,且具有种属和组织差异性。近年来研究结果表明,同工酶对疾病的诊断、跟踪检测、物种的种属鉴定及遗传多样性分析等方面都具有十分重要的意义。主要从同工酶的种类、检测方法及其实际应用等方面进行概述,并着重论述了同工酶在疾病诊断和遗传鉴定中的应用,同时对发展前景进行展望,以期为同工酶的进一步应用提供理论基础。
Isozyme is a kind of biological enzyme, which can catalyze the same biochemical reaction, but its structure and function are different.Recently, research shows that isozyme play an important role in the diagnosis, tracking and detection of diseases, species identification and genetic diversity analysis. In this paper, the species,detection methods and practical applications of isozyme were summarized. The application of isoenzyme in disease diagnosis and genetic identification was discussed, and their development prospects was prospected, in order to provide a theoretical basis for the further application of isozymes.
Isozyme is a kind of biological enzyme, which can catalyze the same biochemical reaction, but its structure and function are different.Recently, research shows that isozyme play an important role in the diagnosis, tracking and detection of diseases, species identification and genetic diversity analysis. In this paper, the species,detection methods and practical applications of isozyme were summarized. The application of isoenzyme in disease diagnosis and genetic identification was discussed, and their development prospects was prospected, in order to provide a theoretical basis for the further application of isozymes.
引文
[1] 王红叶,张娟,蔡焰值,等.白甲鱼不同组织同工酶的组织特异性研究[J].湖北农业科学,2011,50(23):4912-4915.
[2] MARKER C L,MOLLER F.Multiple forms of enzymes:Tissue,notogenetic and species specific patterns[J].Proc Nat Acad Sci USA,1959,45(5):753-763.
[3] 邹春静,盛晓峰,韩文卿,等.同工酶分析技术及其在植物研究中的应用[J].生态学杂志,2003,22(6):63-69.
[4] ZOU L H,SU W,WANG M,et al.Characterization of a functional recombinant human creatine kinase-MB isoenzyme prepared by tandem affinity purification from Escherichia coli[J].Appl Microbiol Biotechnol,2017,101(14):5639-5644.
[5] KIM J,KWON M,MAHARJAN R,et al.Comparisons of morphology and esterase levels between Blattella germanica and Blattella nipponica(Blattodea:Blattellidae)[J].Entomological research,2017,47(3):152-159.
[6] 王晗,韩增华,马银鹏,等.酯酶同工酶鉴定不同地区引种的黑木耳菌株[J].黑龙江科学,2018,9(2):64-66.
[7] 黄宏文,龚俊杰,王圣梅,等.猕猴桃属(Actinidia)植物的遗传多样性[J].生物多样性,2000,8(1):1-12.
[8] 赵田田,蒲宗旺,岳兴建.花斑副沙鳅七种同工酶的组织特异性表达[J].湖北农业科学,2017,56(14):2736-2743.
[9] 洪森荣,周宇瑶,黄慧.上饶早梨离体保存库再生苗遗传稳定性的同工酶分析[J].基因组学与应用生物学,2016,35(12):3552-3561.
[10] 王守训,孙凤祥,李桂芝,等.改良琼脂糖凝胶电泳法测定血清LDH同工酶的应用[J].潍坊医学院学报,2001,23(3):177-178.
[11] WANG Y,WEI L,WEI D B,et al.Enzymatic kinetic properties of the lactate dehydrogenase isoenzyme C4 of the plateau pika(Ochotona curzoniae)[J].Int J Mol Sci,2016,17(1):39-53.
[12] 黄孝湘,李建华,杨明生,等.不同水域中克氏原螯虾主要组织的LDH同工酶分析[J].安徽农业科学,2012,40(25):12536-12538,12540.
[13] 胡明建,郑文寅,张文明,等.利用Native-PAGE鉴定大豆Lox同工酶[J].大豆科学,2012,31(5):813-816.
[14] 韩航,马燕芳,李征珍,等.一种适用于胡杨叶片非变性蛋白质提取的方法[J].科学技术与工程,2018,18(21):36-41.
[15] 夏洪志,杨合同,黄玉杰.聚丙烯酰胺凝胶电泳配合荧光增白剂显色检测木霉几丁质酶同工酶谱[J].中国生物工程杂志,2007,27(6):61-65.
[16] 尹明华,张铭心,李远芳,等.红芽芋低温疗法脱毒苗遗传稳定性同工酶检测[J].分子植物育种,2018,16(8):2571-2576.
[17] 廖海,杜祯,周嘉裕,等.1种利用SDS-PAGE检测过氧化物酶同工酶的分析方法[J].安徽农业科学,2008,36(14):5759-5760,5766.
[18] 刘泽军,江海洪.双向电泳法分离LDH同工酶异常带[J].第三军医大学学报,2000(11):1114-1115.
[19] 张少丽,张鲁刚,张明科.大白菜雄性育性转化相关β-酯酶同工酶双向电泳分析[J].园艺学报,2008,35(5):681-686.
[20] SAMANTA D,MUKHOPADHYAY S.Protein and isozyme analysis in different species,varieties and populations of Tabernaemontana[J].International journal of botany studies,2018,3(1):84-91.
[21] 洪森荣,张铭心,叶思雨,等.高山马铃薯种质资源遗传多样性的同工酶分析[J].浙江农业学报,2018,30(9):1445-1453.
[22] CHIBANI F,SKOURI-GARGOURI H,SALEM A B,et al.Using genetic structure data and phylogenetic criteria in attributing prioritization scores for conservation of spontaneous Capparis spinosa L.populations from Tunisia[J].Journal for nature conservation,2017,37:96-105.
[23] DEWOODY J,ROGERS D L,HIPKINS V D,et al.Spatially explicit and multi-sourced genetic information is critical for conservation of an endangered plant species,San Diego thornmint(Acanthomintha ilicifolia)[J].Conservation genetics,2018,19(4):1-15.
[24] NAKONECHNAYA O V,YATSUNSKAYA M S.Genetic and genotypic variation of Nelumbo komarovii Grossh[J].Russian journal of genetics,2018,54(7):816-824.
[25] 马永平,赵海燕,杨少娟.枸杞多种同工酶水平的遗传多样性分析[J].安徽农业科学,2010,38(8):4042-4043.
[26] 李淑娟,刘艳霞.基于POD同工酶的青藏高原三江源区鹅观草属种质遗传多样性研究[J].分子植物育种,2017,15(11):4724-4729.
[27] 肖慧,崔秀明.三七种内同工酶遗传多样性分析[J].现代中药研究与实践,2010,24(6):16-18.
[28] EL-ESAWI M A,MUSTAFA A E Z,BADR S,et al.Isozyme analysis of genetic variability and population structure of Lactuca L.germplasm[J].Biochemical systematics & ecology,2017,70:73-79.
[29] AHMED S M,MOHAMED A F.Genetic relatedness among Solanum L.species assayed by seed morphology and isozyme markers[J].Pak J Bot,2016,48(5):2011-2016.
[30] ZEMEROV S D,ROOSE B W,GREENBERG M L,et al.Cryptophane nanoscale assemblies expand 129Xe NMR biosensing[J].Analytical chemistry,2018,90(12):7730-7738.
[31] LIU J C,CHEN G,LIU Z Z,et al.Aberrant FGFR tyrosine kinase signaling enhances the Warburg effect by reprogramming LDH isoform expression and activity in prostate cancer[J].Cancer research,2018,78(16):4459-4470.
[32] SONAWANE V K,MAHAJAN U B,SHINDE S D,et al.A chemosensitizer drug:Disulfiram prevents doxorubicin-induced cardiac dysfunction and oxidative stress in rats[J].Cardiovascular toxicology,2018,18(5):459-470.
[33] SHARMA A K,PANDEY S,NERTHIGAN Y,et al.Aggregation of cysteamine-capped gold nanoparticles in presence of ATP as an analytical tool for rapid detection of creatine kinase(CK-MM)[J].Analytica chimica acta,2018,1024:161-168.
[34] MASAMUNE A,HAMADA S,YOSHIDA N,et al.Pyruvate kinase isozyme M2 plays a critical role in the interactions between pancreatic stellate cells and cancer cells[J].Dig Dis Sci,2018,63(7):1868-1877.
[35] LU D H,LV W W,LI W X,et al.High PKM2 expression is independently correlated with decreased overall survival in hepatocellular carcinoma[J].Oncology letters,2018,16(3):3603-3610.
[36] 林娜,于锦香,李艳,等.血清乳酸脱氢酶及其同工酶在复治多发性骨髓瘤中的临床意义[J].中国医科大学学报,2010,39(1):31-33,36.
[37] 张庆莲,张青云.乳酸脱氢酶同工酶在肿瘤诊断中的临床意义[J].中华临床实验室管理电子杂志,2015,3(2):82-86.
[38] 李杨,周飞亚,黄月月,等.3株柔嫩艾美耳球虫安徽株的同工酶检测[J].畜牧与兽医,2016,48(3):113-116.
[39] 郭海林,刘建秀,朱雪花,等.结缕草属植物杂交育种及其杂种鉴定——同工酶的变异分析[J].草业学报,2006,15(6):101-108.
[40] 陶芳,张文明,姚大年.酯酶同工酶鉴定杂交水稻品种及其亲本纯度的研究[J].种子,2007,26(2):28-32.
[2] MARKER C L,MOLLER F.Multiple forms of enzymes:Tissue,notogenetic and species specific patterns[J].Proc Nat Acad Sci USA,1959,45(5):753-763.
[3] 邹春静,盛晓峰,韩文卿,等.同工酶分析技术及其在植物研究中的应用[J].生态学杂志,2003,22(6):63-69.
[4] ZOU L H,SU W,WANG M,et al.Characterization of a functional recombinant human creatine kinase-MB isoenzyme prepared by tandem affinity purification from Escherichia coli[J].Appl Microbiol Biotechnol,2017,101(14):5639-5644.
[5] KIM J,KWON M,MAHARJAN R,et al.Comparisons of morphology and esterase levels between Blattella germanica and Blattella nipponica(Blattodea:Blattellidae)[J].Entomological research,2017,47(3):152-159.
[6] 王晗,韩增华,马银鹏,等.酯酶同工酶鉴定不同地区引种的黑木耳菌株[J].黑龙江科学,2018,9(2):64-66.
[7] 黄宏文,龚俊杰,王圣梅,等.猕猴桃属(Actinidia)植物的遗传多样性[J].生物多样性,2000,8(1):1-12.
[8] 赵田田,蒲宗旺,岳兴建.花斑副沙鳅七种同工酶的组织特异性表达[J].湖北农业科学,2017,56(14):2736-2743.
[9] 洪森荣,周宇瑶,黄慧.上饶早梨离体保存库再生苗遗传稳定性的同工酶分析[J].基因组学与应用生物学,2016,35(12):3552-3561.
[10] 王守训,孙凤祥,李桂芝,等.改良琼脂糖凝胶电泳法测定血清LDH同工酶的应用[J].潍坊医学院学报,2001,23(3):177-178.
[11] WANG Y,WEI L,WEI D B,et al.Enzymatic kinetic properties of the lactate dehydrogenase isoenzyme C4 of the plateau pika(Ochotona curzoniae)[J].Int J Mol Sci,2016,17(1):39-53.
[12] 黄孝湘,李建华,杨明生,等.不同水域中克氏原螯虾主要组织的LDH同工酶分析[J].安徽农业科学,2012,40(25):12536-12538,12540.
[13] 胡明建,郑文寅,张文明,等.利用Native-PAGE鉴定大豆Lox同工酶[J].大豆科学,2012,31(5):813-816.
[14] 韩航,马燕芳,李征珍,等.一种适用于胡杨叶片非变性蛋白质提取的方法[J].科学技术与工程,2018,18(21):36-41.
[15] 夏洪志,杨合同,黄玉杰.聚丙烯酰胺凝胶电泳配合荧光增白剂显色检测木霉几丁质酶同工酶谱[J].中国生物工程杂志,2007,27(6):61-65.
[16] 尹明华,张铭心,李远芳,等.红芽芋低温疗法脱毒苗遗传稳定性同工酶检测[J].分子植物育种,2018,16(8):2571-2576.
[17] 廖海,杜祯,周嘉裕,等.1种利用SDS-PAGE检测过氧化物酶同工酶的分析方法[J].安徽农业科学,2008,36(14):5759-5760,5766.
[18] 刘泽军,江海洪.双向电泳法分离LDH同工酶异常带[J].第三军医大学学报,2000(11):1114-1115.
[19] 张少丽,张鲁刚,张明科.大白菜雄性育性转化相关β-酯酶同工酶双向电泳分析[J].园艺学报,2008,35(5):681-686.
[20] SAMANTA D,MUKHOPADHYAY S.Protein and isozyme analysis in different species,varieties and populations of Tabernaemontana[J].International journal of botany studies,2018,3(1):84-91.
[21] 洪森荣,张铭心,叶思雨,等.高山马铃薯种质资源遗传多样性的同工酶分析[J].浙江农业学报,2018,30(9):1445-1453.
[22] CHIBANI F,SKOURI-GARGOURI H,SALEM A B,et al.Using genetic structure data and phylogenetic criteria in attributing prioritization scores for conservation of spontaneous Capparis spinosa L.populations from Tunisia[J].Journal for nature conservation,2017,37:96-105.
[23] DEWOODY J,ROGERS D L,HIPKINS V D,et al.Spatially explicit and multi-sourced genetic information is critical for conservation of an endangered plant species,San Diego thornmint(Acanthomintha ilicifolia)[J].Conservation genetics,2018,19(4):1-15.
[24] NAKONECHNAYA O V,YATSUNSKAYA M S.Genetic and genotypic variation of Nelumbo komarovii Grossh[J].Russian journal of genetics,2018,54(7):816-824.
[25] 马永平,赵海燕,杨少娟.枸杞多种同工酶水平的遗传多样性分析[J].安徽农业科学,2010,38(8):4042-4043.
[26] 李淑娟,刘艳霞.基于POD同工酶的青藏高原三江源区鹅观草属种质遗传多样性研究[J].分子植物育种,2017,15(11):4724-4729.
[27] 肖慧,崔秀明.三七种内同工酶遗传多样性分析[J].现代中药研究与实践,2010,24(6):16-18.
[28] EL-ESAWI M A,MUSTAFA A E Z,BADR S,et al.Isozyme analysis of genetic variability and population structure of Lactuca L.germplasm[J].Biochemical systematics & ecology,2017,70:73-79.
[29] AHMED S M,MOHAMED A F.Genetic relatedness among Solanum L.species assayed by seed morphology and isozyme markers[J].Pak J Bot,2016,48(5):2011-2016.
[30] ZEMEROV S D,ROOSE B W,GREENBERG M L,et al.Cryptophane nanoscale assemblies expand 129Xe NMR biosensing[J].Analytical chemistry,2018,90(12):7730-7738.
[31] LIU J C,CHEN G,LIU Z Z,et al.Aberrant FGFR tyrosine kinase signaling enhances the Warburg effect by reprogramming LDH isoform expression and activity in prostate cancer[J].Cancer research,2018,78(16):4459-4470.
[32] SONAWANE V K,MAHAJAN U B,SHINDE S D,et al.A chemosensitizer drug:Disulfiram prevents doxorubicin-induced cardiac dysfunction and oxidative stress in rats[J].Cardiovascular toxicology,2018,18(5):459-470.
[33] SHARMA A K,PANDEY S,NERTHIGAN Y,et al.Aggregation of cysteamine-capped gold nanoparticles in presence of ATP as an analytical tool for rapid detection of creatine kinase(CK-MM)[J].Analytica chimica acta,2018,1024:161-168.
[34] MASAMUNE A,HAMADA S,YOSHIDA N,et al.Pyruvate kinase isozyme M2 plays a critical role in the interactions between pancreatic stellate cells and cancer cells[J].Dig Dis Sci,2018,63(7):1868-1877.
[35] LU D H,LV W W,LI W X,et al.High PKM2 expression is independently correlated with decreased overall survival in hepatocellular carcinoma[J].Oncology letters,2018,16(3):3603-3610.
[36] 林娜,于锦香,李艳,等.血清乳酸脱氢酶及其同工酶在复治多发性骨髓瘤中的临床意义[J].中国医科大学学报,2010,39(1):31-33,36.
[37] 张庆莲,张青云.乳酸脱氢酶同工酶在肿瘤诊断中的临床意义[J].中华临床实验室管理电子杂志,2015,3(2):82-86.
[38] 李杨,周飞亚,黄月月,等.3株柔嫩艾美耳球虫安徽株的同工酶检测[J].畜牧与兽医,2016,48(3):113-116.
[39] 郭海林,刘建秀,朱雪花,等.结缕草属植物杂交育种及其杂种鉴定——同工酶的变异分析[J].草业学报,2006,15(6):101-108.
[40] 陶芳,张文明,姚大年.酯酶同工酶鉴定杂交水稻品种及其亲本纯度的研究[J].种子,2007,26(2):28-32.
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