叶尔羌高原鳅三种同工酶的组织差异性研究
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摘要
为了探究叶尔羌高原鳅同工酶表达的组织差异性和基因位点表达特征,试验采用不连续聚丙烯酰胺凝胶电泳技术对养殖和野生个体的心脏、肌肉、肝脏和肾脏4种组织器官的乳酸脱氢酶(LDH)、酯酶(EST)和苹果酸脱氢酶(MDH)进行分离、分析。结果表明:叶尔羌高原鳅LDH由2个基因位点编码,4种组织器官中共检测到3条酶带,组织差异性不明显;EST由3个基因位点编码,4种组织器官中共检测到7条酶带,其中EST-1a和EST-3b为各组织器官共有酶带,具有明显的组织差异性;MDH的S-MDH和M-MDH型同工酶在各组织器官中均有表达,4种组织器官中共检测到5条酶带,但酶带分布具有明显的组织差异性。根据酶谱特征对其种质鉴定进行了分析发现,叶尔羌高原鳅同工酶具有自属的一套同工酶系统特征。
To study the tissue specificity and characteristics of isozymes spectrum in Triplophysa yarkandensis, using the discontinuous polyacrylamide gel electrophoresis, lacated dehydrogenase(LDH), esterase(EST) and malate dehydrogenase(MDH) isozymes from 4 tissues(heart, muscle, liver and kidney) were studied, and the phenotypes of the isozymes and loci were also investigated. The results showed that 3 LDH isozyme bands encoded by 2 gene loci were detected; 7 EST isozyme bands encoded by 3 gene loci were detected and were polymorphism, the EST-1 a and EST-3 b were the basic isozyme bands; the MDH isozymes of S-MDH and M-MDH were expressed and total 5 bands of isozyme in all the 4 tissues of Triplophysa yarkandensis were detected. The EST and MDH isozymes had tissue-specific expression in different degrees. The germplasm identification was analyzed according to the characteristics of isozymes spectrum, it indicatecl that the Triplophysa yarkandensis had it′s characteristics of isozymes system.
To study the tissue specificity and characteristics of isozymes spectrum in Triplophysa yarkandensis, using the discontinuous polyacrylamide gel electrophoresis, lacated dehydrogenase(LDH), esterase(EST) and malate dehydrogenase(MDH) isozymes from 4 tissues(heart, muscle, liver and kidney) were studied, and the phenotypes of the isozymes and loci were also investigated. The results showed that 3 LDH isozyme bands encoded by 2 gene loci were detected; 7 EST isozyme bands encoded by 3 gene loci were detected and were polymorphism, the EST-1 a and EST-3 b were the basic isozyme bands; the MDH isozymes of S-MDH and M-MDH were expressed and total 5 bands of isozyme in all the 4 tissues of Triplophysa yarkandensis were detected. The EST and MDH isozymes had tissue-specific expression in different degrees. The germplasm identification was analyzed according to the characteristics of isozymes spectrum, it indicatecl that the Triplophysa yarkandensis had it′s characteristics of isozymes system.
引文
[1] 陈生熬,马春晖, 丁慧萍,等.塔里木河叶尔羌高原鳅繁殖生物学研究[J]. 水生生物学报, 2013, 37(5):810-816.
[2] ARDESTANI G, ROGELIO D R O, REYES R C, et al. Genetic diversity of two Philippine native freshwater goby species (Perciformes: Gobiidae): implications for conservation[J]. Aquat Conserv, 2015, 24(5):592-600.
[3] LEINEMANN L, STEINER W, HOSIUS B, et al. Genetic variation of chloroplast and nuclear markers in natural populations of hazelnut ( Corylus avellana, L.) in Germany[J].Plant Syst Evol, 2013, 299(2):369-378.
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[5] ZHIGILEVA O N, BARANOVA O G, POZHIDAEV V V, et al. Comparative analysis of using isozyme and Issr-Pcr markers for population differentiation of Cyprinid fish[J]. Turk J Fish Aquat Sci, 2013, 13(1):159-168.
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[8] 于淑池, 邵杨勇. 泥鳅不同组织同工酶的特异性表达分析[J]. 水产科学, 2012, 31(5):291-294.
[9] QVARNSTROM Y, JAMES C, XAYAVONG M, et al. Comparison of real-time PCR protocols for differential laboratory diagnosis of amebiasis[J]. J Clin Microbiol, 2005, 43(11):5491-5497.
[10] 杨从戎, 苗亮, 李明云. 黑鲷不同组织的9种同工酶谱[J]. 生物学杂志, 2014(3):38-42.
[11] 冯为慧, 官少飞, 欧阳敏. 刺鲃同工酶的研究[J]. 安徽农业科学, 2012(7):4029-4031.
[12] 徐钢春, 董晶晶, 聂志娟,等. 刀鲚不同组织的乳酸脱氢酶同工酶及DNA含量研究[J]. 上海海洋大学学报, 2012, 21(4):481-488.
[13] 杨成, 李柯懋, 唐文家,等. 黄河高原鳅乳酸脱氢酶同工酶研究[J]. 水利渔业, 2008, 28(4):41-42.
[14] 张娟, 王红叶, 王玉凤,等. 岩原鲤三种同工酶的组织特异性研究[J]. 湖北农业科学, 2012, 51(11):2307-2310.
[15] WU Y L, DU Q Y. Tissue specificity analysis of isozyme in male and female of misgurnus anguillicaudatus[J]. Acta Agric Boreali-Occident Sin, 2008, 17(6):36-40.
[16] 区又君, 齐旭东, 李加儿. 波纹唇鱼不同组织5种同工酶表达的差异[J]. 南方水产科学, 2009, 5(2):51-55.
[17] 肖调义, 陈清华, 陈开健,等. 四种黄颡鱼乳酸脱氢酶同工酶电泳的研究[J]. 上海海洋大学学报, 2004, 13(1):72-74.
[18] 汪新颖, 王波, 侯松涛,等. 苹果酸脱氢酶的结构及功能[J]. 生物学杂志, 2009, 26(4):69-72.
[19] 李娴, 王成武, 安丽,等. 淡水黑鲷5种同工酶的组织特异性研究[J]. 中国农学通报, 2011, 27(7):380-384.
[20] JANKO K, FLAJ H M, CHOLEVA L, et al. Diversity of European spined loaches (genus Cobitis L.): an update of the geographic distribution of the Cobitis taenia hybrid complex with a description of new molecular tools for species and hybrid determination[J]. J Fish Biol, 2007,71(5):387-408.
[21] 周宗汉, 林金榜. 血清乳酸脱氢酶同功酶圆盘电泳分离法在鱼类分类工作中的应用[J]. 动物学杂志, 1983, 14(5):36-38.
[22] 尹绍武, 黄海, 廖经球,等. 点带石斑鱼与褐点石斑鱼4种同工酶的比较研究[J]. 水产科学, 2007, 26(9):500-503.
[23] ALARCON J A, ALVAREZ M C. Genetic identification of sparid species by isozyme markers: application to interspecific hybrids[J]. Aquaculture, 1999, 173(3):95-103.
[24] 韩庆, 赵东海. 洞庭湖区不同体色鲇不同组织同工酶的比较研究[J]. 广东农业科学, 2015, 42(2):104-107.
[25] NAKORN U, SODSUK P, WONGRAT P, et al. Isozyme variation among four species of the catfish genus Clarias[J]. J Fish Biol, 2002, 60(4):1051-1057.
[26] 肖义军, 高建民, 傅文庆. 大鳞副泥鳅LDH同工酶的发育遗传学研究[J]. 遗传, 1996,18(6):6-8.
[2] ARDESTANI G, ROGELIO D R O, REYES R C, et al. Genetic diversity of two Philippine native freshwater goby species (Perciformes: Gobiidae): implications for conservation[J]. Aquat Conserv, 2015, 24(5):592-600.
[3] LEINEMANN L, STEINER W, HOSIUS B, et al. Genetic variation of chloroplast and nuclear markers in natural populations of hazelnut ( Corylus avellana, L.) in Germany[J].Plant Syst Evol, 2013, 299(2):369-378.
[4] 刘鸿艳, 谢从新. 鱼类同工酶应用及研究进展[J]. 水生态学杂志, 2006, 26(5):1-3.
[5] ZHIGILEVA O N, BARANOVA O G, POZHIDAEV V V, et al. Comparative analysis of using isozyme and Issr-Pcr markers for population differentiation of Cyprinid fish[J]. Turk J Fish Aquat Sci, 2013, 13(1):159-168.
[6] 熊全沫. 鱼类同工酶谱分析(上)[J]. 遗传, 1992, 14(2):41-44.
[7] 熊全沫. 鱼类同工酶谱分析(下)[J]. 遗传, 1992, 14(3):47-48.
[8] 于淑池, 邵杨勇. 泥鳅不同组织同工酶的特异性表达分析[J]. 水产科学, 2012, 31(5):291-294.
[9] QVARNSTROM Y, JAMES C, XAYAVONG M, et al. Comparison of real-time PCR protocols for differential laboratory diagnosis of amebiasis[J]. J Clin Microbiol, 2005, 43(11):5491-5497.
[10] 杨从戎, 苗亮, 李明云. 黑鲷不同组织的9种同工酶谱[J]. 生物学杂志, 2014(3):38-42.
[11] 冯为慧, 官少飞, 欧阳敏. 刺鲃同工酶的研究[J]. 安徽农业科学, 2012(7):4029-4031.
[12] 徐钢春, 董晶晶, 聂志娟,等. 刀鲚不同组织的乳酸脱氢酶同工酶及DNA含量研究[J]. 上海海洋大学学报, 2012, 21(4):481-488.
[13] 杨成, 李柯懋, 唐文家,等. 黄河高原鳅乳酸脱氢酶同工酶研究[J]. 水利渔业, 2008, 28(4):41-42.
[14] 张娟, 王红叶, 王玉凤,等. 岩原鲤三种同工酶的组织特异性研究[J]. 湖北农业科学, 2012, 51(11):2307-2310.
[15] WU Y L, DU Q Y. Tissue specificity analysis of isozyme in male and female of misgurnus anguillicaudatus[J]. Acta Agric Boreali-Occident Sin, 2008, 17(6):36-40.
[16] 区又君, 齐旭东, 李加儿. 波纹唇鱼不同组织5种同工酶表达的差异[J]. 南方水产科学, 2009, 5(2):51-55.
[17] 肖调义, 陈清华, 陈开健,等. 四种黄颡鱼乳酸脱氢酶同工酶电泳的研究[J]. 上海海洋大学学报, 2004, 13(1):72-74.
[18] 汪新颖, 王波, 侯松涛,等. 苹果酸脱氢酶的结构及功能[J]. 生物学杂志, 2009, 26(4):69-72.
[19] 李娴, 王成武, 安丽,等. 淡水黑鲷5种同工酶的组织特异性研究[J]. 中国农学通报, 2011, 27(7):380-384.
[20] JANKO K, FLAJ H M, CHOLEVA L, et al. Diversity of European spined loaches (genus Cobitis L.): an update of the geographic distribution of the Cobitis taenia hybrid complex with a description of new molecular tools for species and hybrid determination[J]. J Fish Biol, 2007,71(5):387-408.
[21] 周宗汉, 林金榜. 血清乳酸脱氢酶同功酶圆盘电泳分离法在鱼类分类工作中的应用[J]. 动物学杂志, 1983, 14(5):36-38.
[22] 尹绍武, 黄海, 廖经球,等. 点带石斑鱼与褐点石斑鱼4种同工酶的比较研究[J]. 水产科学, 2007, 26(9):500-503.
[23] ALARCON J A, ALVAREZ M C. Genetic identification of sparid species by isozyme markers: application to interspecific hybrids[J]. Aquaculture, 1999, 173(3):95-103.
[24] 韩庆, 赵东海. 洞庭湖区不同体色鲇不同组织同工酶的比较研究[J]. 广东农业科学, 2015, 42(2):104-107.
[25] NAKORN U, SODSUK P, WONGRAT P, et al. Isozyme variation among four species of the catfish genus Clarias[J]. J Fish Biol, 2002, 60(4):1051-1057.
[26] 肖义军, 高建民, 傅文庆. 大鳞副泥鳅LDH同工酶的发育遗传学研究[J]. 遗传, 1996,18(6):6-8.
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