眼柄切除对口虾蛄卵巢、肝胰脏和肌肉生化组分的影响
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摘要
采用镊烫法切除体长10.20~15.20cm雌性口虾蛄单侧眼柄,测量性腺指数和肝胰腺指数,测定卵巢、肝胰脏和肌肉中的生化组分含量的变化,以探讨眼柄切除对口虾蛄卵巢发育的影响。结果显示,切除单侧眼柄后,口虾蛄性腺指数上升,切除后第5、10、15d极显著高于对照组(P<0.01)。试验组和对照组口虾蛄的肝胰腺指数在试验后期均显著下降(P<0.05)。试验组卵巢中的糖原含量高于对照组,在第5、15d分别与对照组间具有显著(P<0.05)和极显著差异(P<0.01);肝胰脏中的糖原含量也显著或极显著高于对照组(P<0.05和P<0.01);而肌肉中的糖原含量自第5d开始下降,早于对照组。切除单侧眼柄第10d后,肝胰脏中蛋白含量开始显著上升(P<0.05),之后均高于对照组;试验组卵巢中蛋白含量也高于对照组,第15d时差异具有统计学意义;而试验组与对照组肌肉中的蛋白含量均表现出先升后降趋势,二者间差异不显著(P>0.05)。试验结果表明,眼柄切除可以促进口虾蛄体内营养物质积累和转移,有助于卵巢发育。
The gonadosomatic index(GSI),hepatosomatic index(HPI)and biochemical compositions of ovary,hepatopancreas and muscle were investigated in female mantis shrimp Oratosquilla oratoria with body length of 10.20—15.20 cm by a hot tweezers method to research the effects of unilateral eyestalk ablation on ovary development.The results showed that the mantis shrimp in the treatment group had significantly higher GSI than the mantis shrimp in the control group did on 5,10,and 15 days after eyestalk ablation(P<0.01).HPI was found to be significantly decreased in both treatment and control groups in the late stage of the whole experiment(P<0.05).There were higher glycogen levels in ovary and hepatopancreas of the mantis shrimp in the treatment group than in the control group,with significant difference in 5 days(P<0.05)and very significant difference in 15 days(P<0.01).In muscle,however,the glycogen level was shown to be decreased in the treatment group in 5 days,earlier than that in the control group.In the ablated eyestalk mantis shrimp,the hepatopancreas protein content was elevated in 10 days and then significantly higher than that in the mantis shrimp in the control group(P<0.05).There was higher protein content in ovary in the treatment group than that in the control group,with significant difference in 15 days(P<0.05).The elevated first and then declined protein contents in ovary were observed in both groups,without significant difference(P>0.05).The findings indicate that eyestalk ablation promotes nutrient accumulation and transformation,and is contributed to the development of ovary in mantis shrimp.
The gonadosomatic index(GSI),hepatosomatic index(HPI)and biochemical compositions of ovary,hepatopancreas and muscle were investigated in female mantis shrimp Oratosquilla oratoria with body length of 10.20—15.20 cm by a hot tweezers method to research the effects of unilateral eyestalk ablation on ovary development.The results showed that the mantis shrimp in the treatment group had significantly higher GSI than the mantis shrimp in the control group did on 5,10,and 15 days after eyestalk ablation(P<0.01).HPI was found to be significantly decreased in both treatment and control groups in the late stage of the whole experiment(P<0.05).There were higher glycogen levels in ovary and hepatopancreas of the mantis shrimp in the treatment group than in the control group,with significant difference in 5 days(P<0.05)and very significant difference in 15 days(P<0.01).In muscle,however,the glycogen level was shown to be decreased in the treatment group in 5 days,earlier than that in the control group.In the ablated eyestalk mantis shrimp,the hepatopancreas protein content was elevated in 10 days and then significantly higher than that in the mantis shrimp in the control group(P<0.05).There was higher protein content in ovary in the treatment group than that in the control group,with significant difference in 15 days(P<0.05).The elevated first and then declined protein contents in ovary were observed in both groups,without significant difference(P>0.05).The findings indicate that eyestalk ablation promotes nutrient accumulation and transformation,and is contributed to the development of ovary in mantis shrimp.
引文
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[37]罗荣生,王幽兰,曹梅讯.中华绒螯蟹血淋巴20-羟基蜕皮酮诱发蜕皮和卵巢发育的作用[J].动物学报,1999,36(2):157-164.
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[2]Shimizu T.On the resource of Japanese mantis shrimp Oratosquilla oratoria(de Haan)in Tokyo Bay—Ⅰ:summarization on the resource utilization and life history[J].Bulletin of the Kanagawa Prefectural Fisheries Research Institute,2002(7):1-10.
[3]Kodama K,Shimizu T,Yamakawa T,et al.Reproductive biology of the female Japanese mantis shrimp Oratosquilla oratoria(Stomatopoda)in relation to changes in the seasonal pattern of larval occurrence in Tokyo Bay,Japan[J].Fisheries Science,2004,70(5):734-745.
[4]林月娇,刘海映,徐海龙,等.大连近海口虾蛄形态参数关系的研究[J].大连海洋大学学报,2008,23(3):215-217.
[5]Zhang D,Ding G,Ge B,et al.Development and characterization of microsatellite loci of Oratosquilla oratoria(Crustacea:Squillidae)[J].Conservation Genetics Resources,2011,4(4):147-150.
[6]王春琳.黑斑口虾蛄的繁殖生物学研究[D].杭州:浙江大学,2011.
[7]徐善良,王春琳,梅文骧,等.浙江北部海区口虾蛄繁殖和摄食习性的初步研究[J].浙江水产学院学报,1996,15(1):21-29.
[8]刘海映,谷德贤,姜玉声,等.口虾蛄繁殖周期及生殖细胞发育的研究[J].大连海洋大学学报,2013,28(3):269-272.
[9]薛梅,闫红伟,刘海映,等.大连皮口海域口虾蛄群体繁殖生物学特征初步研究[J].大连海洋大学学报,2016,31(3):237-245.
[10]Ohtomi J,Shimizu M.Spawning season of the Japanese mantis shrimp Oratosquilla oratoria in Tokyo Bay[J].Nippon Suisan Gakkaishi,1988,54(11):1929-1933.
[11]Yamazaki M,Fuji A.Reproductive cycle of the mantis shrimp,Squilla oratoria de Haan,in Mutsu Bay[J].Bulletin of the Faculty of Fisheries Hokkaido University,1980,31(2):161-168.
[12]Yan H W,Xue M,Liu H Y,et al.Energy reserves and gonad steroid levels during the reproductive cycle of the Japanese mantis shrimp Oratosquilla oratoria De Haan,1844(Stomatopoda:Squillidae)in Pikou Bay,Dalian,China[J].Journal of Crustacean Biology,2017,37(1),99-108.
[13]刘海映,张娜,闫红伟,等.口虾蛄眼柄卵黄蛋白原(Vg)基因的初步研究[J].大连海洋大学学报,2016,31(2):131-139.
[14]Hamano T,Matsuura S.Egg laying and egg mass nursing behaviour in the Japanese mantis shrimp[J].Nippon Suisan Gakkaishia,1984,50(12):1969-1973.
[15]Hamano T,Matsuura S.Food habits of the Japanese mantis shrimp in the benthic communty of Hakata Bay[J].Nippon Suisan Gakkaishia,1986,52(5):787-794.
[16]Hamano T,Matsuura S.Egg size,duration of incubation,and larval development of the Japanese mantis shrimp in the laboratory[J].Nippon Suisan Gakkaishia,1987,53(1):23-39.
[17]Hamano T.Growth of the stomatopod crustacean Oratosquillaoratoria in Hakate Bay[J].Nippon Suisan Gakkaishia,1990,56(9):1529.
[18]王波,张锡烈.口虾蛄人工育苗生产技术[J].齐鲁渔业,1998,15(6):14-16.
[19]孙丕喜,张锡烈.口虾蛄人工育苗技术研究[J].黄渤海海洋,2000,18(2):41-46.
[20]王春琳,叶选怡,丁爱侠,等.虾蛄繁殖生物学与繁殖技术研究[J].海洋湖沼通报,2002(3):58-64.
[21]蒋敏霞,王春琳,赵青松,等.黑斑口虾蛄育苗技术研究[J].海洋科学,2004,24(5):17-20.
[22]刘海映.口虾蛄池塘生态育苗方法:中国,200910220089.1[P].2013-04-24.
[23]Panouse J B.Influence de I′ablation de pedoncleoculaire sur la croissance de I′ovaire chez la crevette Leander serralus[J].Comptes Rendus de I′Academic des Science,1943(217):553-555.
[24]王芳,穆迎春,董双林,等.去眼柄对凡纳滨对虾稚虾蜕皮和生长的影响[J].中国海洋大学学报:自然科学版,2004,34(3):371-376.
[25]赵朝阳,周鑫,徐增洪,等.克氏原螯虾人工诱导繁殖的初步研究[J].经济动物学报,2008,12(4):222-230.
[26]张宽.眼柄切除对中华绒螯蟹肝胰腺和生殖腺蛋白质含量及其种类的影响[J].山西农业大学学报,2006,26(2):128-131.
[27]Sroyraya M,Chotwiwatthanakun C,Stewart M J,et al.Biolateral eyestalk ablation of the blue swimmer crab,Portunus pelagicus,produces hypertrophy of the androgenic gland and an increase of cells producing insulin-like androgenic gland hormone[J].Tissue and Cell,2010,42(5):293-300.
[28]吴超,林琼武,张黎黎,等.饥饿和切除眼柄对日本囊对虾亲虾性腺发育及血淋巴生化成分含量的影响[J].厦门大学学报:自然科学版,2009,48(5):750-755.
[29]Beltz B.Endocrinology of selected invertebrate types[G]//Laufer H,Downer R G H.Crustacean endocrinology,New York:Alan R Liss,1988:235-258.
[30]Cooke I M,Sullivan R E.Hormones and neurosecretion[G]//At wood,H L,Sandeman D C.Biology of Crustacea,New York:Academic Press,1982:205-290.
[31]Keller R.Crustacean neuropeptides:structures,functions and comparative aspects[J].Experimentia,1992,48(5):439-448.
[32]Bomirski A,Arendarezyk M,Kawinska E,et al.Partial characterization of crustacean gonad-inhibiting hormone[J].International J of Invertebrate Reproduction,1981(3):213-219.
[33]Dekleijin D V.Localization of messenger RNAs encoding crustacean hyperglycemic hormone and gonad inhibiting hormone in the X-organ sinus gland complex of the lobster Homanus americanus[J].Neuroscience,1992,15(1):121-128.
[34]DeKleijin D,Janswsen K,Waddy S,et al.Expression of the crustacean hyperglycaemic hormones and the gonad-inhibiting hormone during the reproductive cycle of the female American lobster Homarus americanus[J].Journal of Endocrinol,1998,156(2):291-298.
[35]Warrier S R,Tirumalai R,Subramoniam T.Occurrence of vertebrate steroids,estradiol 17beta and progesterone in the reproducing females of the mud crab Scylla serrata[J].Comp Biochem Physiol,A Mol Integr Physiol,2001,130(2):283-294.
[36]Alikunhi K,Poernomo A,Adisukresno S,et al.Preliminary observations on induction of maturity and spawning in Penaeus monodon Fabricius and Penaeus merguiensis de Man by eye-stalk extirpation[J].Bulletin of the Shrimp Culture Research Centre,1975,1(1):1-11.
[37]罗荣生,王幽兰,曹梅讯.中华绒螯蟹血淋巴20-羟基蜕皮酮诱发蜕皮和卵巢发育的作用[J].动物学报,1999,36(2):157-164.
[38]邓思平,叶满,朱春华,等.切除眼柄对凡纳滨对虾亲虾卵巢、肝胰腺、性腺、血清生化组成的影响[J].广东海洋大学学报,2016,36(3):30-34.
[39]王春琳,郑春静,蒋敏霞,等.黑斑口虾蛄人工育苗技术研究[J].中国水产科学,2000,7(3):67-70.
[40]Harrison E K.Broodstock nutrition and maturation diets[G]//D′Abramo L R,Conklin D E,Akiyama D M.Crustacean Nutrition,Baton Rouge:World Aquacultule Society,1997:390-410.
[41]Gehring W R.Maturational changes in the ovarian lipid spectrum of the pink shrimp Penaeus duorarum Burkenroad[J].Comp Biochem Physiol A Comp Physiol,1994,49(3):511-524.
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