龙血竭总黄酮纯化工艺研究
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摘要
目的建立龙血竭总黄酮的纯化工艺。方法以龙血素A、龙血素B的吸附率和解吸率为评价指标,采用大孔树脂纯化龙血竭总黄酮,确定最佳纯化工艺。结果大孔树脂纯化龙血竭总黄酮的最佳工艺为:选择D101大孔树脂,用0.3%NaOH溶液溶解龙血竭乙酸乙酯提取物,上样质量浓度为12 mg/mL,上样量为2 BV,上样体积流量为1.0 BV/h,依次用纯化水洗脱杂质至流出液呈中性为止,再用80%乙醇5 BV洗脱。得到的龙血竭总黄酮含量以龙血素A和龙血素B计,比药材中含量提高了3倍。结论 D101大孔树脂可用于龙血竭总黄酮的纯化,该工艺稳定可靠。
Objective To establish a purification process for total flavonoids from Dragon's blood. Methods The adsorption rate and desorption rate of Loureirin A and Loureirin B were used to evaluate the purification process. The macroporous adsorption resin was used to purify the total flavonoids of Dragon's blood, and the best process was determined. Results The optimal process for the purification of total flavonoids from Dragon's blood by macroporous adsorption resin was as follows: D101 macroporous resin was selected, and the ethyl acetate extract of Dragon's blood was dissolved in 0.3% NaOH solution. The concentration of the sample was 12 mg/mL. The loading amount was 2 BV. The loading volume was 1.0 BV/h. The impurities were eluted with purified water until the effluent was neutral,and then eluted with 80% ethanol 5 BV to obtain the total flavonoids of Dragon's blood. The contents of Loureirin A and Loureirin B was increased by 3 times compared with the materia medica. Conclusion D101 macroporous resin can be used in the purification process of total flavonoids from Dragon's blood. The process is stable and reliable.
Objective To establish a purification process for total flavonoids from Dragon's blood. Methods The adsorption rate and desorption rate of Loureirin A and Loureirin B were used to evaluate the purification process. The macroporous adsorption resin was used to purify the total flavonoids of Dragon's blood, and the best process was determined. Results The optimal process for the purification of total flavonoids from Dragon's blood by macroporous adsorption resin was as follows: D101 macroporous resin was selected, and the ethyl acetate extract of Dragon's blood was dissolved in 0.3% NaOH solution. The concentration of the sample was 12 mg/mL. The loading amount was 2 BV. The loading volume was 1.0 BV/h. The impurities were eluted with purified water until the effluent was neutral,and then eluted with 80% ethanol 5 BV to obtain the total flavonoids of Dragon's blood. The contents of Loureirin A and Loureirin B was increased by 3 times compared with the materia medica. Conclusion D101 macroporous resin can be used in the purification process of total flavonoids from Dragon's blood. The process is stable and reliable.
引文
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[12]刘芳,戴荣继,邓玉林,等.龙血竭总酚活血化瘀活性成分的虚拟筛选及初步活性研究[J].北京理工大学学报,2015,35(2):218-220.
[13]陈素,吴水才,曾毅,等.龙血竭总黄酮抗炎镇痛作用及其镇痛机制探讨[J].时珍国医国药,2013,24(5):1030-1032.
[14]屠鹏飞,王钰芳,邵杰,等.龙血竭中黄酮类成分提取工艺研究[J].中国实验方剂学杂志,2011,17(6):30-32.
[2]ZHENG Q A,LI H Z,ZHANG Y J,et al.Flavonoids from the resin of Dracaena cochinchinensis[J].Helvetica Chimica Acta,2004,87(5):1167-1171.
[3]LUO Y,DAI H F,WANG H,et al.Chemical constituents from dragon's blood of Dracaena cambodiana[J].Chinese Journal of Natural Medicines,2011,9(2):112-114.
[4]MORALES-SERNA J A,JIMENEZ A,ESTRADA-REYES R,et al.Homoiso flavanones from Agave tequilana Weber[J].Molecules,2010,15(5):3295-3301.
[5]MEKSURIYEN D,CORDELL G A.Retrodihydrochalcones from Dracaena Loureiri[J].Journal of Natural Products,1988,51(6):1129-1135.
[6]周志宏,王锦亮,杨崇仁.国产血竭的化学成分研究[J].中草药,2001,32(6):484.
[7]卢文杰,吕扬.剑叶龙血树氯仿部位化学成分的研究[J].药学学报,1998,3(10):755-758.
[8]王锦亮,阮德春,程治英,等.剑叶龙血树树脂中的植物防卫素[J].应用生态学报,1999,10(2):255-256.
[9]韦宏,文东旭,刘晓松.广西血竭石油醚和醋酸乙酯部位中的化学成分[J].广西中医药,1993,16(1):38-39.
[10]屠鹏飞,陶晶,胡迎庆,等.龙血竭黄酮类成分研究[J].中国天然药物,2003,3(1):45-46.
[11]郑庆安.龙血竭研究[D].昆明:中国科学院昆明植物研究所,2003.
[12]刘芳,戴荣继,邓玉林,等.龙血竭总酚活血化瘀活性成分的虚拟筛选及初步活性研究[J].北京理工大学学报,2015,35(2):218-220.
[13]陈素,吴水才,曾毅,等.龙血竭总黄酮抗炎镇痛作用及其镇痛机制探讨[J].时珍国医国药,2013,24(5):1030-1032.
[14]屠鹏飞,王钰芳,邵杰,等.龙血竭中黄酮类成分提取工艺研究[J].中国实验方剂学杂志,2011,17(6):30-32.