光合产电微生物群落构建及光照培养对其产电性能的影响
|
摘要
采用淡水沉积物为接种来源,培养出光合产电微生物群落。将其与藻阴极联用组建了完整的光合作用微生物燃料电池时,功率密度达到(157.5±3.1)m W/m2。采用循环伏安法及电化学阻抗谱对该群落的电化学性能进行了测试。PCR-DGGE及紫外可见吸收光谱分析显示,该群落含有Ectothiorhodospiraceae科及Chloroflexi门不产氧光合细菌、产电菌Arcobacter butzleri、发酵细菌及其他细菌。对该群落进行长期黑暗培养或长期光照培养时,其产电性能均得到了提高,但功率密度测试显示,光照培养微生物燃料电池最大功率密度为(180.1±8.7)m W/m2,高于黑暗培养的微生物燃料电池(160.7±11.4)m W/m2。电化学测试也显示,光照培养的阳极产电性能优于黑暗培养的阳极。
A natural electrogenic microbial community,which included anoxygenic phototrophic bacteria,was isolated from a freshwater deposit. When this community was used in a photosynthetic microbial fuel cell( MFC) with an algal cathode,the maximum power density achieved was( 157. 5 ± 3. 1) m W/m2. Electrochemical performance of the anode was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy tests. PCR-DGGE and UV-vis analysis indicated the existence of Ectothiorhodospiraceae,Chloroflexi,Arcobacter butzleri,fermentative bacteria,and other bacteria in the microbial community. Electrochemical performance of the microbial community improved by long-term operation,both with and without illumination; the maximum power densities of the MFC with and without illumination were( 180. 1 ± 8. 7) m W / m2and( 160. 7 ± 11. 4)m W / m2,respectively. In addition,the electrochemical tests confirmed that the performance of the anode cultivated under illumination was better than that of the anode cultivated in the dark.
A natural electrogenic microbial community,which included anoxygenic phototrophic bacteria,was isolated from a freshwater deposit. When this community was used in a photosynthetic microbial fuel cell( MFC) with an algal cathode,the maximum power density achieved was( 157. 5 ± 3. 1) m W/m2. Electrochemical performance of the anode was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy tests. PCR-DGGE and UV-vis analysis indicated the existence of Ectothiorhodospiraceae,Chloroflexi,Arcobacter butzleri,fermentative bacteria,and other bacteria in the microbial community. Electrochemical performance of the microbial community improved by long-term operation,both with and without illumination; the maximum power densities of the MFC with and without illumination were( 180. 1 ± 8. 7) m W / m2and( 160. 7 ± 11. 4)m W / m2,respectively. In addition,the electrochemical tests confirmed that the performance of the anode cultivated under illumination was better than that of the anode cultivated in the dark.
引文
[1]Logan B.E.,Hamelers B.,Rozendal R.,et al.Microbial fuel cells:Methodology and technology.Environmental Science&Technology,2006,40(17):5181-5192
[2]Logan B.E.,Regan J.M.Microbia fuel cells:Challenges and applications.Environmental Science&Technology,2006,40(17):5172-5180
[3]Rosenbaum M.,He Z.,Angenent L.T.Light energy to bioelectricity:Photosynthetic microbial fuel cells.Current Opinion in Biotechnology,2010,21(3):259-264
[4]Xiao Li,He Zhen.Applications and perspectives of phototrophic microorganisms for electricity generation from organic compounds in microbial fuel cells.Renewable and Sustainable Energy Reviews,2014,37:550-559
[5]Ei Mekawy A.,Hegab H.M.,Vanbroekhoven K.,et al.Techno-productive potential of photosynthetic microbial fuel cells through different configurations.Renewable and Sustainable Energy Reviews,2014,39:617-627
[6]杨素萍,林志华,崔小华,等.不产氧光合细菌的分类学进展.微生物学报,2008,48(11):1562-1566Yang Suping,Lin Zhihua,Cui Xiaohua,et al.Current taxonomy of anoxygenic phototrophic bacteria:A review.Acta Microbiologica Sinica,2008,48(11):1562-1566(in Chinese)
[7]Xing Defeng,Zuo Yi,Cheng Shaoan,et al.Electricity generation by Rhodopseudomonas palustris DX-1.Environmental Science&Technology,2008,42(11):4146-4151
[8]Adessi A.,Torzillo G.,Baccetti E.,et al.Sustained outdoor H2production with Rhodopseudomonas palustris cultures in a 50 L tubular photobioreactor.International Journal of Hydrogen Energy,2012,37(10):8840-8849
[9]Keisuke M.,Makoto Y.,Akihiro F.Improving the performance of a direct photosynthetic/metabolic bio-fuel cell(DPBFC)using gene manipulated bacteria.Micromech Microeng,2007,17:S274-S279
[10]Badalamenti J.P.,Torres C.I.,Krajmalnik-Brown R.Light-responsive current generation by phototrophically enriched anode biofilms dominated by green sulfur bacteria.Biotechnology and Bioengineering,2013,110(4):1020-1027
[11]Cao Xiaoxin,Huang Xia,Boon N.,et al.Electricity generation by an enriched phototrophic consortium in a microbial fuel cell.Electrochemistry Communications,2008,10(9):1392-1395
[12]曹效鑫.微生物燃料电池中产电菌与电极的作用机制及其应用.北京:清华大学博士学位论文,2009Cao Xiaoxin.Mechanism and application of exoelectro-genelectrode interaction in microbial fuel cell.Beijing:Doctor Dissertation of Tsinghua University,2009(in Chinese)
[13]吴义诚,肖勇,赵峰.一株光合细菌的分离鉴定及其产电特性.环境工程学报,2014,8(10):4503-4507Wu Yicheng,Xiao Yong,Zhao Feng.Isolation and identification of a photosynthetic bacteria and its electricity-generating characteristics.Chinese Journal of Environmental Engineering,2014,8(10):4503-4507(in Chinese)
[14]Chandra R.,Venkata Subhash G.,Venkata Mohan S.Mixotrophic operation of photo-bioelectrocatalytic fuel cell under anoxygenic microenvironment enhances the light dependent bioelectrogenic activity.Bioresource Technology,2012,109:46-56
[15]王兴祖,程翔,孙德智,等.利用MFC阳极室暗发酵培养沼泽红假单胞菌.环境工程学报,2014,8(10):4492-4496Wang Xingzu,Cheng Xiang,Sun Dezhi,et al.Dark culture of Rhodopseudomonas palustris using MFC reactor.Chinese Journal of Environmental Engineering,2014,8(10):4492-4496(in Chinese)
[16]Gouveia L.,Neves C.,Sebastio D.,et al.Effect of light on the production of bioelectricity and added-value microalgae biomass in a photosynthetic alga microbial fuel cell.Bioresource Technology,2014,154:171-177
[17]Wu Yicheng,Wang Zejie,Zheng Yue,et al.Light intensity affects the performance of photo microbial fuel cells with Desmodesmus sp.A8 as cathodic microorganism.Applied Energy,2014,116:86-90
[18]刘如林,刁虎欣,梁风来.光合细菌及其应用.北京:中国农业科学技术出版社,1991
[19]Zhao Guang,Ma Fang,Li Wei,et al.Electricity generation from cattle dung using microbial fuel cell technology during anaerobic acidogenesis and the development of microbial populations.Waste Management,2012,32(9):1651-1658
[20]Ishii S.,Shimoyama T.,Hotta Y.,et al.Characterization of a filamentous biofilm community established in a cellulose-fed microbial fuel cell.BMC Microbiology,2008,8:6
[21]Fedorovich V.,Knighton M.C.,Pagaling E.,et al.Novel electrochemically active bacterium phylogenetically related to Arcobacter butzleri,isolated from a microbial fuel cell.Applied and Environmental Microbiology,2009,75(23):7326-7334
[22]Heylen K.,Lebbe L.,De Vos P.Acidovorax caeni sp.nov.,a denitrifying species with genetically diverse isolates from activated sludge.International Journal of Systematic and Evolutionary Microbiology,2008,58:73-77
[2]Logan B.E.,Regan J.M.Microbia fuel cells:Challenges and applications.Environmental Science&Technology,2006,40(17):5172-5180
[3]Rosenbaum M.,He Z.,Angenent L.T.Light energy to bioelectricity:Photosynthetic microbial fuel cells.Current Opinion in Biotechnology,2010,21(3):259-264
[4]Xiao Li,He Zhen.Applications and perspectives of phototrophic microorganisms for electricity generation from organic compounds in microbial fuel cells.Renewable and Sustainable Energy Reviews,2014,37:550-559
[5]Ei Mekawy A.,Hegab H.M.,Vanbroekhoven K.,et al.Techno-productive potential of photosynthetic microbial fuel cells through different configurations.Renewable and Sustainable Energy Reviews,2014,39:617-627
[6]杨素萍,林志华,崔小华,等.不产氧光合细菌的分类学进展.微生物学报,2008,48(11):1562-1566Yang Suping,Lin Zhihua,Cui Xiaohua,et al.Current taxonomy of anoxygenic phototrophic bacteria:A review.Acta Microbiologica Sinica,2008,48(11):1562-1566(in Chinese)
[7]Xing Defeng,Zuo Yi,Cheng Shaoan,et al.Electricity generation by Rhodopseudomonas palustris DX-1.Environmental Science&Technology,2008,42(11):4146-4151
[8]Adessi A.,Torzillo G.,Baccetti E.,et al.Sustained outdoor H2production with Rhodopseudomonas palustris cultures in a 50 L tubular photobioreactor.International Journal of Hydrogen Energy,2012,37(10):8840-8849
[9]Keisuke M.,Makoto Y.,Akihiro F.Improving the performance of a direct photosynthetic/metabolic bio-fuel cell(DPBFC)using gene manipulated bacteria.Micromech Microeng,2007,17:S274-S279
[10]Badalamenti J.P.,Torres C.I.,Krajmalnik-Brown R.Light-responsive current generation by phototrophically enriched anode biofilms dominated by green sulfur bacteria.Biotechnology and Bioengineering,2013,110(4):1020-1027
[11]Cao Xiaoxin,Huang Xia,Boon N.,et al.Electricity generation by an enriched phototrophic consortium in a microbial fuel cell.Electrochemistry Communications,2008,10(9):1392-1395
[12]曹效鑫.微生物燃料电池中产电菌与电极的作用机制及其应用.北京:清华大学博士学位论文,2009Cao Xiaoxin.Mechanism and application of exoelectro-genelectrode interaction in microbial fuel cell.Beijing:Doctor Dissertation of Tsinghua University,2009(in Chinese)
[13]吴义诚,肖勇,赵峰.一株光合细菌的分离鉴定及其产电特性.环境工程学报,2014,8(10):4503-4507Wu Yicheng,Xiao Yong,Zhao Feng.Isolation and identification of a photosynthetic bacteria and its electricity-generating characteristics.Chinese Journal of Environmental Engineering,2014,8(10):4503-4507(in Chinese)
[14]Chandra R.,Venkata Subhash G.,Venkata Mohan S.Mixotrophic operation of photo-bioelectrocatalytic fuel cell under anoxygenic microenvironment enhances the light dependent bioelectrogenic activity.Bioresource Technology,2012,109:46-56
[15]王兴祖,程翔,孙德智,等.利用MFC阳极室暗发酵培养沼泽红假单胞菌.环境工程学报,2014,8(10):4492-4496Wang Xingzu,Cheng Xiang,Sun Dezhi,et al.Dark culture of Rhodopseudomonas palustris using MFC reactor.Chinese Journal of Environmental Engineering,2014,8(10):4492-4496(in Chinese)
[16]Gouveia L.,Neves C.,Sebastio D.,et al.Effect of light on the production of bioelectricity and added-value microalgae biomass in a photosynthetic alga microbial fuel cell.Bioresource Technology,2014,154:171-177
[17]Wu Yicheng,Wang Zejie,Zheng Yue,et al.Light intensity affects the performance of photo microbial fuel cells with Desmodesmus sp.A8 as cathodic microorganism.Applied Energy,2014,116:86-90
[18]刘如林,刁虎欣,梁风来.光合细菌及其应用.北京:中国农业科学技术出版社,1991
[19]Zhao Guang,Ma Fang,Li Wei,et al.Electricity generation from cattle dung using microbial fuel cell technology during anaerobic acidogenesis and the development of microbial populations.Waste Management,2012,32(9):1651-1658
[20]Ishii S.,Shimoyama T.,Hotta Y.,et al.Characterization of a filamentous biofilm community established in a cellulose-fed microbial fuel cell.BMC Microbiology,2008,8:6
[21]Fedorovich V.,Knighton M.C.,Pagaling E.,et al.Novel electrochemically active bacterium phylogenetically related to Arcobacter butzleri,isolated from a microbial fuel cell.Applied and Environmental Microbiology,2009,75(23):7326-7334
[22]Heylen K.,Lebbe L.,De Vos P.Acidovorax caeni sp.nov.,a denitrifying species with genetically diverse isolates from activated sludge.International Journal of Systematic and Evolutionary Microbiology,2008,58:73-77