DNA条形码在原生动物系统分类学中的应用
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摘要
DNA条形码技术是利用目的基因中一段标准的、有足够变异的且相对较短的DNA序列来进行物种快速与准确鉴定的一种分子生物学技术。DNA条形码技术历经十几年的发展,在动物、植物和微生物等领域的物种分类和鉴定研究中得到应用。本文从DNA条形码的概念和特点、DNA条形码在原生动物系统分类学研究中的应用等方面进行了综合介绍,以期拓展中小学生的知识面,促进中小学素质教育的发展。
DNA barcoding is a rapid and accurate molecular method for species identification based on a standard,sufficiently variable and relatively short DNA fragments in the target gene.After more than ten years of development,DNA barcoding has been applied for species classification and identification in the fields of animals,plants and microorganisms.In this article,we reviewed the conception and features of DNA barcoding,and described its application in phylogenetic taxonomy of protozoa,which aimed to supply the knowledge of primary and secondary school students and promote the development of quality education in primary and secondary schools.
DNA barcoding is a rapid and accurate molecular method for species identification based on a standard,sufficiently variable and relatively short DNA fragments in the target gene.After more than ten years of development,DNA barcoding has been applied for species classification and identification in the fields of animals,plants and microorganisms.In this article,we reviewed the conception and features of DNA barcoding,and described its application in phylogenetic taxonomy of protozoa,which aimed to supply the knowledge of primary and secondary school students and promote the development of quality education in primary and secondary schools.
引文
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[2]李琪,邹山梅,郑小东,等.DNA条形码及其在海洋生物中的应用[J].中国海洋大学学报(自然科学版),2010,40(8):43-47.
[3]赵研.纤毛虫DNA条形码候选基因的研究[D].中国海洋大学,2014.
[4]徐武杰,汪雁,邹节新,等.DNA条形码及其在寄生虫学中的应用[J].中国人兽共患病学报,2015,31(4):365-370.
[5]Anush Kosakyan,Fatma Gomaa,Edward A.D.Mitchell,et al.Using DNA-barcoding for sorting out protist species complex-es:A case study of the Nebela tincta–collaris–bohemicagroup(Amoebozoa;Arcellinida,Hyalospheniidae)[J].EuropeanJournal of Protistology,2013,49(2):222-237.
[6]Adams E R,Hamilton P B,Malele I I,et al.The identification,diversity and prevalence of trypanosomes in field caught tsetsein Tanzania using ITS-1 primers and fluorescent fragmentlength barcoding[J].Infection Genetics&Evolution,2008,8(4):439-444.
[7]李建华,张西臣,田宗成,等.火鸡隐孢子虫18S核糖体DNA部分序列测定与系统发育分析[J].中国预防兽医学报,2002,24(4):267-269.
[8]Ogedengbe J D,Hanner R H,Barta J R.DNA barcoding iden-tifies Eimeria species and contributes to the phylogenetics ofcoccidian parasites(Eimeriorina,Apicomplexa,Alveolata)[J].In-ternational Journal for Parasitology,2011,41(8):843-850.
[9]王鑫.鸡艾美耳球虫纯株的建立与DNA条形编码初探[D].中国农业科学院,2007.
[10]Pawlowski J,Audic S,Adl S,et al.CBOL protist workinggroup:barcoding eukaryotic richness beyond the animal,plant,and fungal kingdoms[J].Plos Biology,2012,10(11):e1001419.
[2]李琪,邹山梅,郑小东,等.DNA条形码及其在海洋生物中的应用[J].中国海洋大学学报(自然科学版),2010,40(8):43-47.
[3]赵研.纤毛虫DNA条形码候选基因的研究[D].中国海洋大学,2014.
[4]徐武杰,汪雁,邹节新,等.DNA条形码及其在寄生虫学中的应用[J].中国人兽共患病学报,2015,31(4):365-370.
[5]Anush Kosakyan,Fatma Gomaa,Edward A.D.Mitchell,et al.Using DNA-barcoding for sorting out protist species complex-es:A case study of the Nebela tincta–collaris–bohemicagroup(Amoebozoa;Arcellinida,Hyalospheniidae)[J].EuropeanJournal of Protistology,2013,49(2):222-237.
[6]Adams E R,Hamilton P B,Malele I I,et al.The identification,diversity and prevalence of trypanosomes in field caught tsetsein Tanzania using ITS-1 primers and fluorescent fragmentlength barcoding[J].Infection Genetics&Evolution,2008,8(4):439-444.
[7]李建华,张西臣,田宗成,等.火鸡隐孢子虫18S核糖体DNA部分序列测定与系统发育分析[J].中国预防兽医学报,2002,24(4):267-269.
[8]Ogedengbe J D,Hanner R H,Barta J R.DNA barcoding iden-tifies Eimeria species and contributes to the phylogenetics ofcoccidian parasites(Eimeriorina,Apicomplexa,Alveolata)[J].In-ternational Journal for Parasitology,2011,41(8):843-850.
[9]王鑫.鸡艾美耳球虫纯株的建立与DNA条形编码初探[D].中国农业科学院,2007.
[10]Pawlowski J,Audic S,Adl S,et al.CBOL protist workinggroup:barcoding eukaryotic richness beyond the animal,plant,and fungal kingdoms[J].Plos Biology,2012,10(11):e1001419.