细胞代谢组学研究方法和应用
|
摘要
细胞代谢直接或间接参与细胞活动的各个方面,代谢组学技术能够检测和鉴定内源性生化反应产物,精确揭示细胞内代谢途径和过程的信息。细胞代谢组学作为代谢组学研究的一个新兴的方向,在疾病诊断、毒理学研究、药理机制和细胞培养微环境等多个领域都有所应用。
Cell metabolism is directly or indirectly involved in all aspects of cellular activity, and metabolomics technology can detect and identify endogenous biochemical reaction products and accurately reveal intracellular information about metabolic pathways and processes. Cell metabonomics is a new direction of metabonomics research and has been applied in many fields such as disease diagnosis, toxicology research, pharmacological mechanism cell culture microenvironment and so on.
Cell metabolism is directly or indirectly involved in all aspects of cellular activity, and metabolomics technology can detect and identify endogenous biochemical reaction products and accurately reveal intracellular information about metabolic pathways and processes. Cell metabonomics is a new direction of metabonomics research and has been applied in many fields such as disease diagnosis, toxicology research, pharmacological mechanism cell culture microenvironment and so on.
引文
[1] Nicholson JK, Lindon JC, Holmes E. ‘Metabonomics’: understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J]. Xenobiotica, 1999, 29: 1181-1189.
[2] Ramirez T, Strigun A, Verlohner A, et al. Prediction of liver toxicity and mode of action using metabolomics in vitro in HepG2 cells[J]. Arch Toxicology, 2018, 92: 893-906.
[3] Gao L,Wang KX,Zhang NN,et al. 1H nuclear magnetic resonance based M-etabolomics approach reveals the metabolic mechanism of (-)-5-Hydroxy-equol against hepatocellular carcinoma cells in vitro[J].J Proteome Res,2018,17:1833-1843.
[4] Klontzas ME, Vernardis SI, Heliotis M, et al. Metabolomics analysis of the osteogenic differentiation of umbilical cord blood mesenchymal stem cells reveals differential sensitivity to osteogenic agents[J]. Stem Cells Dev, 2017, 26: 723-733.
[5] Martins C,Brand?o T,Almeida A. Metabolomics strategy for the mapping of volatile exometabolome from Saccharomyces spp widely used in the food industry based on comprehensive two-dimensional gas chromatography[J].J Sep Sci,2017,40:2228-2237.
[6] Hernández Bort JA,Shanmukam V,Pabst M,et al.Reduced quenching and extraction time for mammalian cells using filtration and syringe extraction[J]. J Biotechnol,2014,182-183:97-103.
[7] Kapoore RV,Coyle R,Staton CA,et al.Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: a case study with the metastatic breast cancer cell line MDA-MB-231[J].Analyst,2017,142:2038-2049.
[8] Ser Z,Liu X,Tang NN.Extraction parameters for metabolomics from cultured cells[J].Anal Biochem,2015,475:22-28.
[9] Wang J,Westenskow PD,Fang M,et al.Quantitative metabolomics of photore-ceptor degeneration and the effects of stem cell-derived retinal pigment epit-helium transplantation[J].Philos Trans A Math Phys Eng Sci,2016,374.doi:10.1098/rsta.2015.0376.
[10] Markley JL,Brüschweiler R,Edison AS,et al.The future of NMR-based meta-bolomics[J].Curr Opin Biotechnol,2017,43:34-40.
[11] Wang H,Chen J,Feng Y,et al.(1)H nuclear magnetic resonance-based extrac-ellular metabolomic analysis of multidrug resistant Tca8113 oral squamous car-cinoma cells[J].Oncol Lett,2015,9:2551-2559.
[12] Li JZ,Qu H,Wu J,et al.Metabolic profiles of adipose-derived and bone mar-row-derived stromal cells from elderly coronary heart disease patients by capi-llary liquid chromatography quadrupole time-of-flight mass spectrometry[J].Int J Mol Med,2018,41:184-194.
[13] Kalluri U,Naiker M.Cell culture metabolomics in the diagnosis of lung can-cer-the influence of cell culture condi-tions[J].J Breath Res,2014,8:027109.DOI:10.1088/1752-7155/8/2/027109.
[14] Silva CL,Perestrelo R,Silva P,et al.Volatile metabolomic signature of human breast cancer cell lines[J].Sci Rep,2017,7:43969.doi:10.1038/srep43969.
[15] Rezola A,Pey J,Rubio á.In-silico prediction of key metabolic differences be-tween two non-small cell lung cancer subtypes[J].PLoS One,2014,9:e103998.doi:10.1371/journal.pone.0103998.
[16] Fan TW,Yuan P,Lane AN,et al.Stable isotope-resolved metabolomic analysis of lithium effects on glial-neuronal metabolism and interactions[J].Metabolom-ics,2010,6:165-179.
[17] St?ubert C,Bhuiyan H,Lindahl A,et al.Rewired metabolism in drug-resistant leukemia cells: a metabolic switch hallmarked by reduced dependence on ex-ogenous glutamine[J].J Biol Chem,2015,290:8348-8359.
[18] Soliman GA,Steenson SM.Effects of Metformin and a Mammalian Target of Rapamycin (mTOR) ATP-Competitive Inhibitor on Targeted Metabolomics in Pancreatic Cancer Cell Line[J].Metabolomics (Los Angel),2016,6.doi:10.4172/2153-0769.1000183.
[19] Forristal CE,Christensen DR,Chinnery FE,et al.Environmental oxygen tension regulates the energy metabolism and self-renewal of human embryonic stem cells[J].PLoS One,2013,8:e62507.doi:10.1371/journal.pone.0062507.
[20] Turner J,Quek LE,Titmarsh D,et al.Metabolic profiling and flux analysis of MEL-2 human embryonic stem cells during exponential growth at physiological and atmospheric oxygen concentrations[J].PLoS One,2014,9:e112757.doi:10.1371/journal.pone.0112757.
[21] D’Alessandro A,Nemkov T,Hansen KC,et al.Red blood cell storage in additi-ve solution-7 preserves energy and redox metabolism: a metabolomics approa-ch[J].Transfusion,2015,55:2955-2966.
[22] García-Caňaveras JC,Castell JV,Donato MT.A metabolomics cell-based appro-ach for anticipating and investigat-ing drug-induced liver injury[J].Sci Rep,2016,6:27239.doi:10.1038/srep27239.
[2] Ramirez T, Strigun A, Verlohner A, et al. Prediction of liver toxicity and mode of action using metabolomics in vitro in HepG2 cells[J]. Arch Toxicology, 2018, 92: 893-906.
[3] Gao L,Wang KX,Zhang NN,et al. 1H nuclear magnetic resonance based M-etabolomics approach reveals the metabolic mechanism of (-)-5-Hydroxy-equol against hepatocellular carcinoma cells in vitro[J].J Proteome Res,2018,17:1833-1843.
[4] Klontzas ME, Vernardis SI, Heliotis M, et al. Metabolomics analysis of the osteogenic differentiation of umbilical cord blood mesenchymal stem cells reveals differential sensitivity to osteogenic agents[J]. Stem Cells Dev, 2017, 26: 723-733.
[5] Martins C,Brand?o T,Almeida A. Metabolomics strategy for the mapping of volatile exometabolome from Saccharomyces spp widely used in the food industry based on comprehensive two-dimensional gas chromatography[J].J Sep Sci,2017,40:2228-2237.
[6] Hernández Bort JA,Shanmukam V,Pabst M,et al.Reduced quenching and extraction time for mammalian cells using filtration and syringe extraction[J]. J Biotechnol,2014,182-183:97-103.
[7] Kapoore RV,Coyle R,Staton CA,et al.Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: a case study with the metastatic breast cancer cell line MDA-MB-231[J].Analyst,2017,142:2038-2049.
[8] Ser Z,Liu X,Tang NN.Extraction parameters for metabolomics from cultured cells[J].Anal Biochem,2015,475:22-28.
[9] Wang J,Westenskow PD,Fang M,et al.Quantitative metabolomics of photore-ceptor degeneration and the effects of stem cell-derived retinal pigment epit-helium transplantation[J].Philos Trans A Math Phys Eng Sci,2016,374.doi:10.1098/rsta.2015.0376.
[10] Markley JL,Brüschweiler R,Edison AS,et al.The future of NMR-based meta-bolomics[J].Curr Opin Biotechnol,2017,43:34-40.
[11] Wang H,Chen J,Feng Y,et al.(1)H nuclear magnetic resonance-based extrac-ellular metabolomic analysis of multidrug resistant Tca8113 oral squamous car-cinoma cells[J].Oncol Lett,2015,9:2551-2559.
[12] Li JZ,Qu H,Wu J,et al.Metabolic profiles of adipose-derived and bone mar-row-derived stromal cells from elderly coronary heart disease patients by capi-llary liquid chromatography quadrupole time-of-flight mass spectrometry[J].Int J Mol Med,2018,41:184-194.
[13] Kalluri U,Naiker M.Cell culture metabolomics in the diagnosis of lung can-cer-the influence of cell culture condi-tions[J].J Breath Res,2014,8:027109.DOI:10.1088/1752-7155/8/2/027109.
[14] Silva CL,Perestrelo R,Silva P,et al.Volatile metabolomic signature of human breast cancer cell lines[J].Sci Rep,2017,7:43969.doi:10.1038/srep43969.
[15] Rezola A,Pey J,Rubio á.In-silico prediction of key metabolic differences be-tween two non-small cell lung cancer subtypes[J].PLoS One,2014,9:e103998.doi:10.1371/journal.pone.0103998.
[16] Fan TW,Yuan P,Lane AN,et al.Stable isotope-resolved metabolomic analysis of lithium effects on glial-neuronal metabolism and interactions[J].Metabolom-ics,2010,6:165-179.
[17] St?ubert C,Bhuiyan H,Lindahl A,et al.Rewired metabolism in drug-resistant leukemia cells: a metabolic switch hallmarked by reduced dependence on ex-ogenous glutamine[J].J Biol Chem,2015,290:8348-8359.
[18] Soliman GA,Steenson SM.Effects of Metformin and a Mammalian Target of Rapamycin (mTOR) ATP-Competitive Inhibitor on Targeted Metabolomics in Pancreatic Cancer Cell Line[J].Metabolomics (Los Angel),2016,6.doi:10.4172/2153-0769.1000183.
[19] Forristal CE,Christensen DR,Chinnery FE,et al.Environmental oxygen tension regulates the energy metabolism and self-renewal of human embryonic stem cells[J].PLoS One,2013,8:e62507.doi:10.1371/journal.pone.0062507.
[20] Turner J,Quek LE,Titmarsh D,et al.Metabolic profiling and flux analysis of MEL-2 human embryonic stem cells during exponential growth at physiological and atmospheric oxygen concentrations[J].PLoS One,2014,9:e112757.doi:10.1371/journal.pone.0112757.
[21] D’Alessandro A,Nemkov T,Hansen KC,et al.Red blood cell storage in additi-ve solution-7 preserves energy and redox metabolism: a metabolomics approa-ch[J].Transfusion,2015,55:2955-2966.
[22] García-Caňaveras JC,Castell JV,Donato MT.A metabolomics cell-based appro-ach for anticipating and investigat-ing drug-induced liver injury[J].Sci Rep,2016,6:27239.doi:10.1038/srep27239.