亚热带水果中糖组分的测定及分析
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摘要
目的建立离子色谱测定亚热带水果中果糖、葡萄糖、肌醇等糖类的检测方法,并分析各种水果的糖组分相似性。方法采用万通离子色谱仪(Metrohm 850 Professional IC)和万通离子色谱柱(Metrosep Carb 2-250/4.0),100mmol/L NaOH+10mmol/L CH3COONa为淋洗液,以安培检测器(工作电极为金,参比电极为钯)测定水果中的糖类。结果亚热带水果中果糖含量为0.58～17.47g/100g,葡萄糖含量为0.39～20.45 g/100g,肌醇含量为0～0.25 g/100g,蔗糖含量为0～12.17 g/100g,山梨糖含量为0～1.99 g/100g,麦芽糖含量为0～0.10 g/100g。样品加标回收率均在95.5%～122.3%,精密度为1.80%～10.15%。结论通过对亚热带水果中糖组分相关性分析,发现葡萄糖和果糖为亚热带水果中糖类的主要成分;通过聚类分析可以显示不同品种的水果有相似的糖组分,同一品种水果的总糖含量相近,却有着不同的糖组分。[营养学报,2019,41(3):308-312]
Objective A high performance Ion chromatographic method was developed to determine fructose,glucose, inositol and other 3 kinds of sugars in subtropical fruits. Methods The polysaccharides in subtropical fruits were separated by ion chromatographic column(Metrosep Carb 2-250/4.0) and ion chromatography(Metrohm 850 Professional IC). 100 mmol/L NaOH + 10 mmol/L CH3 COONa were used for elution and an amperometric detector was used for determining the sugar content in fruits. Results The content of fructose in subtropical fruits was 0.58-17.47 g/100 g, the glucose content ranged from 0.39 to 20.45 g/100 g, the inositol content from 0 to 0.25 g/100 g, the sucrose content from 0 to12.17 g/100 g, the sorbose content from 0 to1.99 g/100 g, and the maltose content from 0 to 0.10 g/100 g. The recovery of the test samples was 95.5%-122.3%, and the precision was 1.80%-10.15%. Conclusion Through the determination of sugar components and the correlation analysis of sugar components in subtropical fruits, glucose and fructose are the main components of sugars in subtropical fruits. By cluster analysis, the fruits of different varieties are similar in sugar components. The fruits of the same varieties are similar in total sugar content, but different in sugar components. [ACTA NUTRIMENTA SINICA, 2019,41(3):308-312]
Objective A high performance Ion chromatographic method was developed to determine fructose,glucose, inositol and other 3 kinds of sugars in subtropical fruits. Methods The polysaccharides in subtropical fruits were separated by ion chromatographic column(Metrosep Carb 2-250/4.0) and ion chromatography(Metrohm 850 Professional IC). 100 mmol/L NaOH + 10 mmol/L CH3 COONa were used for elution and an amperometric detector was used for determining the sugar content in fruits. Results The content of fructose in subtropical fruits was 0.58-17.47 g/100 g, the glucose content ranged from 0.39 to 20.45 g/100 g, the inositol content from 0 to 0.25 g/100 g, the sucrose content from 0 to12.17 g/100 g, the sorbose content from 0 to1.99 g/100 g, and the maltose content from 0 to 0.10 g/100 g. The recovery of the test samples was 95.5%-122.3%, and the precision was 1.80%-10.15%. Conclusion Through the determination of sugar components and the correlation analysis of sugar components in subtropical fruits, glucose and fructose are the main components of sugars in subtropical fruits. By cluster analysis, the fruits of different varieties are similar in sugar components. The fruits of the same varieties are similar in total sugar content, but different in sugar components. [ACTA NUTRIMENTA SINICA, 2019,41(3):308-312]
引文
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[2]Szajdek A,Borowska EJ.Bioactive compounds and health-promoting properties of berry fruits:a review[J].Plant Foods Hum Nutr,2008,63:147-156.
[3]Medeiros PM,Simoneit BRT.Analysis of sugars in environmental samples by gas chromatography-mass spectrometry[J].J Chromatogr A,2007,1141:271-278.
[4]Molnár-Perl I.Simultaneous quantitation of acids and sugars by chromatography:gas or high-performance liquid chromatography?[J].J Chromatogr A,1999,845:181-195.
[5]Marquez-Sillero I,Cardenas S,Valcarcel M.Comparison of two evaporative universaldetectors for the determination of sugars in food samples by liquid chromatography[J].Microchem J,2013,110:629-635.
[6]Agblevor FA,Hames BR,Schell D,et al.Analysis of biomass sugars using a novel HPLC method[J].Appl Biochem Biotechnol,2007,136:309-326.
[7]Andersen R,S?rensen A.Separation and determination of alditols and sugars by high-p H anion-exchange chromatography with pulsed amperometric detection[J].J Chromatogr A,2000,897:195-204.
[8]Wang X,Chen Y.Determination of carbohydrates as their p-sulfophenylhydrazones by capillary zone electrophoresis[J].Carbohydr Res,2001,332:191-196.
[9]Rouseff RL,Martin SF.Sugar levels in canned single strength grapefruit juice from Florida[J].Proc Florida State Hortic Soc,1985,98:198-200.
[10]Riby JE,Fujisawa T,Kretchmer N.Fructose absorption[J].Am J Clin Nutr,1993,58:748S-753S.
[11]王镜岩.生物化学[M].北京:高等教育出版社,2002:15.