不动杆菌与假单胞菌对17β-雌二醇的协同降解特性
|
摘要
分别用纯培养与模拟培养法考察不动杆菌LM1与假单胞菌LY1组成的复合菌对17β-雌二醇(E2)的协同降解特性.结果表明:当细胞数量比为1时,复合菌对E2的降解率分别为菌株LM1和LY1单独作用的2.2倍和2.6倍,降解过程中菌株LM1和LY1的细胞数量比趋于1;在28℃,150r/min条件下培养7d后,复合菌对初始质量浓度为1,5,10,20mg/L的E2降解率分别为99.6%,97%,93%,85%;当培养基中氯化钠添加的质量分数为3.5%时,复合菌对E2的降解率显著下降(p<0.05);在25℃时复合菌对鸡粪、猪粪及土壤样品中E2的降解率分别为对照组的2.7,3.0,2.8倍.即由菌株LM1和LY1组成的复合菌可通过协同作用降解E2.
The synergistic degradation characteristics of 17β-estradiol(E2)by compound bacteria containing Acinetobacter LM1 and Pseudomonas LY1 were investigated by using pure and simulated culture methods,respectively.The results show that when the cell number ratio was 1,the degradation rate of E2 by compound bacteria was 2.2 and 2.6 times higer than that of strain LM1 and LY1,respectively,and the cell number ratio of strain LM1 and LY1 tended to be 1 in the process of degradation.The degradation rates of E2 with initial mass concentrations of 1,5,10,20 mg/L compound bacteria were 99.6%,97%,93%,85% under conditions of 28 ℃ and 150 r/min,respectively.The degradation rate of E2 was significantly decreased by compound bacteria when the mass fraction of NaCl was 3.5% in the culture media(p<0.05).The degradation rates of E2 in chicken manure,pig manure and soil samples by compound bacteria were 2.7,3.0 and 2.8 times higerthan that of the control group at 25 ℃.Therefore,the compound bacteria composed of strains LM1 and LY1 could degrade E2 by synergistic action.
The synergistic degradation characteristics of 17β-estradiol(E2)by compound bacteria containing Acinetobacter LM1 and Pseudomonas LY1 were investigated by using pure and simulated culture methods,respectively.The results show that when the cell number ratio was 1,the degradation rate of E2 by compound bacteria was 2.2 and 2.6 times higer than that of strain LM1 and LY1,respectively,and the cell number ratio of strain LM1 and LY1 tended to be 1 in the process of degradation.The degradation rates of E2 with initial mass concentrations of 1,5,10,20 mg/L compound bacteria were 99.6%,97%,93%,85% under conditions of 28 ℃ and 150 r/min,respectively.The degradation rate of E2 was significantly decreased by compound bacteria when the mass fraction of NaCl was 3.5% in the culture media(p<0.05).The degradation rates of E2 in chicken manure,pig manure and soil samples by compound bacteria were 2.7,3.0 and 2.8 times higerthan that of the control group at 25 ℃.Therefore,the compound bacteria composed of strains LM1 and LY1 could degrade E2 by synergistic action.
引文
[1]李明堂,林泳墨.畜禽养殖排放内源类固醇雌激素的污染与控制研究进展[J].吉林农业大学学报,2014,36(3):253-257.(LI Mingtang,LIN Yongmo.Research Advances in Pollution and Control of Endogenous Steroid Estrogens Discharged by Livestock and Poultry Production[J].Journal of Jilin Agricultural University,2014,36(3):253-257.)
[2]史艳艳,刘庆生,范志影,等.雌激素质谱筛查分析方法的研究[J].饲料工业,2015,36(24):49-54.(SHI Yanyan,LIU Qingsheng,FAN Zhiying,et al.The Study of Screening Analysis Mehtod of Estrogen Mass Spectrometry[J].Feed Industry,2015,36(24):49-54.)
[3]Laune P B,de,Moore F P A,Jr.17β-Estradiol in Runoff as Affected by Various Poultry Litter Application Strategies[J].Science of the Total Environment,2013,444:26-31.
[4]Chen T S,Chen T C,Yehkuei J C,et al.High Esrtogen Concentrations in Receiving River Discharge from a Concentrated Livestock Feedlot[J].Science of the Total Environment,2010,408:3223-3230.
[5]Mariailaria V,Ermelinda L,Rosaria S.Estrogenic Contamination by Manure Fertilizer in Organic Farming:A Case Study with the Lizard Podarcis sicula[J].Ecotoxicology,2016,25(10):105-144.
[6]Khan S J,Roser D J,Davies C M,et al.Chemical Contaminants in Feedlot Wastes:Concentrations,Effects and Attenuation[J].Environ Int,2008,34:839-859.
[7]Blnquez P,Guieysse B.Continuous Biodegradation of 17β-Estradiol and 17α-Ethynylestradiol by Trametes versicolor[J].Hazard Mater,2008,150(2):459-462.
[8]Futoshi K,Maki O,Satoshi S,et al.Degradation of Natural Estrogen and Identification of the Metabolites Produced by Soil Isolates of Rhodococcus sp.and Sphingomonas sp.[J].Journal of Bioscience and Bioengineering,2010,109(6):576-582.
[9]YU Changping,Ron H K,CHU Kunghui.17β-Estradiol-Degrading Bacteria Isolated from Activated Sludge[J].Environ Sci Technol,2007,41:486-492.
[10]JIANG Liying,YANG Jun,CHEN Jianmeng.Isolation and Characteristics of 17β-Estradiol-Degrading Bacillus spp.Strains from Activated Sludge[J].Biodegradation,2010,21(5):729-736.
[11]李明堂,田来明,王呈玉,等.睾丸酮丛毛单胞菌PhaR基因敲除菌株的构建及其3,17β-HSD对甾族化合物的响应特性[J].环境科学学报,2014,34(11):2773-2778.(LING Mingtang,TIAN Laiming,WANG Chengyu,et al.Construction of PhaR Gene Knock-Out Mutant of Comamonas testosteroni and Responses of Its 3,17β-HSD to Steroid Compounds[J].Acta Scientiae Circumstantiae,2014,34(11):2773-2778.)
[12]YU Changping,Deeb R A,CHU Kunghui.Microbial Degradation of Steroidal Estrogens[J].Chenosphere,2013,91(9):1225-1235.
[13]Zhang T,Xiong G,Maser E,et al.A Novel Transcriptional Repressor PhaRfor the Steroid-Inducible Expression of the 3,17β-Hydroxysteroid Dehydrogenase Gene in Comamonastestosteroni ATCC11996[J].Chem-Biol Interact,2013,202(1/2/3):116-125.
[14]李明堂,徐镜波,卢振兰,等.两株细菌对邻氯硝基苯的协同降解[J].环境科学学报,2010,30(6):1138-1143.(LI Mingtang,XU Jingbo,LU Zhenlan,et al.Synergistic Degradation of 2-Chloronitrobenzene by Two Strains[J].Journal of Environmental Sciences,2010,30(6):1138-1143.)
[15]Isabelle M,Villemur R,Juteau P,et al.Isolation of Estrogen-Degrading Bacteria from an Activated Sludge Bioreactor Treating Swine Waste,Including a Strain That Converts Estrone toβ-Estradiol[J].Canadian Journal of Microbiol,2011,57(7):559-568.
[16]Bram P,Klaas W,Herlinde N,et al.17α-Ethinylestradiol Cometabolism by Bacteria Degrading Estrone,17β-Estradioland Estriol[J].Biodegradation,2008,19(5):683-693.
[17]王晓明.鸡粪常规营养成分分析及其开发利用[J].湖北农业科学,2013,52(21):5314-5316.(WANG Xiaoming.Analysis of Nutritional Components of Chicken Manure and Its Exploitation[J].Journal of Hubei Agricultural Sciences,2013,52(21):5314-5316.)
[2]史艳艳,刘庆生,范志影,等.雌激素质谱筛查分析方法的研究[J].饲料工业,2015,36(24):49-54.(SHI Yanyan,LIU Qingsheng,FAN Zhiying,et al.The Study of Screening Analysis Mehtod of Estrogen Mass Spectrometry[J].Feed Industry,2015,36(24):49-54.)
[3]Laune P B,de,Moore F P A,Jr.17β-Estradiol in Runoff as Affected by Various Poultry Litter Application Strategies[J].Science of the Total Environment,2013,444:26-31.
[4]Chen T S,Chen T C,Yehkuei J C,et al.High Esrtogen Concentrations in Receiving River Discharge from a Concentrated Livestock Feedlot[J].Science of the Total Environment,2010,408:3223-3230.
[5]Mariailaria V,Ermelinda L,Rosaria S.Estrogenic Contamination by Manure Fertilizer in Organic Farming:A Case Study with the Lizard Podarcis sicula[J].Ecotoxicology,2016,25(10):105-144.
[6]Khan S J,Roser D J,Davies C M,et al.Chemical Contaminants in Feedlot Wastes:Concentrations,Effects and Attenuation[J].Environ Int,2008,34:839-859.
[7]Blnquez P,Guieysse B.Continuous Biodegradation of 17β-Estradiol and 17α-Ethynylestradiol by Trametes versicolor[J].Hazard Mater,2008,150(2):459-462.
[8]Futoshi K,Maki O,Satoshi S,et al.Degradation of Natural Estrogen and Identification of the Metabolites Produced by Soil Isolates of Rhodococcus sp.and Sphingomonas sp.[J].Journal of Bioscience and Bioengineering,2010,109(6):576-582.
[9]YU Changping,Ron H K,CHU Kunghui.17β-Estradiol-Degrading Bacteria Isolated from Activated Sludge[J].Environ Sci Technol,2007,41:486-492.
[10]JIANG Liying,YANG Jun,CHEN Jianmeng.Isolation and Characteristics of 17β-Estradiol-Degrading Bacillus spp.Strains from Activated Sludge[J].Biodegradation,2010,21(5):729-736.
[11]李明堂,田来明,王呈玉,等.睾丸酮丛毛单胞菌PhaR基因敲除菌株的构建及其3,17β-HSD对甾族化合物的响应特性[J].环境科学学报,2014,34(11):2773-2778.(LING Mingtang,TIAN Laiming,WANG Chengyu,et al.Construction of PhaR Gene Knock-Out Mutant of Comamonas testosteroni and Responses of Its 3,17β-HSD to Steroid Compounds[J].Acta Scientiae Circumstantiae,2014,34(11):2773-2778.)
[12]YU Changping,Deeb R A,CHU Kunghui.Microbial Degradation of Steroidal Estrogens[J].Chenosphere,2013,91(9):1225-1235.
[13]Zhang T,Xiong G,Maser E,et al.A Novel Transcriptional Repressor PhaRfor the Steroid-Inducible Expression of the 3,17β-Hydroxysteroid Dehydrogenase Gene in Comamonastestosteroni ATCC11996[J].Chem-Biol Interact,2013,202(1/2/3):116-125.
[14]李明堂,徐镜波,卢振兰,等.两株细菌对邻氯硝基苯的协同降解[J].环境科学学报,2010,30(6):1138-1143.(LI Mingtang,XU Jingbo,LU Zhenlan,et al.Synergistic Degradation of 2-Chloronitrobenzene by Two Strains[J].Journal of Environmental Sciences,2010,30(6):1138-1143.)
[15]Isabelle M,Villemur R,Juteau P,et al.Isolation of Estrogen-Degrading Bacteria from an Activated Sludge Bioreactor Treating Swine Waste,Including a Strain That Converts Estrone toβ-Estradiol[J].Canadian Journal of Microbiol,2011,57(7):559-568.
[16]Bram P,Klaas W,Herlinde N,et al.17α-Ethinylestradiol Cometabolism by Bacteria Degrading Estrone,17β-Estradioland Estriol[J].Biodegradation,2008,19(5):683-693.
[17]王晓明.鸡粪常规营养成分分析及其开发利用[J].湖北农业科学,2013,52(21):5314-5316.(WANG Xiaoming.Analysis of Nutritional Components of Chicken Manure and Its Exploitation[J].Journal of Hubei Agricultural Sciences,2013,52(21):5314-5316.)