豆粕发酵物改善小鼠睡眠功效及其与天麻的协同作用
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摘要
本文研究了大豆粕枯草芽孢杆菌发酵物、天麻及其两者的混合物对改善小鼠睡眠功效的影响。以2.5 g/kg·BW·d剂量的豆粕发酵物、天麻和其两者1∶1混合物每日灌胃ICR小鼠,对照组灌胃等体积蒸馏水,14d后进行直接睡眠试验、戊巴比妥钠阈下剂量催眠试验、巴比妥钠睡眠潜伏期试验和延长戊巴比妥钠睡眠时间试验,同时记录小鼠体重和脏器指数,用于观察小鼠及其脏器健康程度。试验结果表明,豆粕发酵物、天麻和豆粕发酵物-天麻混合物均有增加小鼠入睡率(P<0.05)的作用,灌胃单一的豆粕发酵物没有明显缩短小鼠睡眠潜伏期的作用,灌胃单一天麻也没有明显延长小鼠睡眠时间的作用。然而,豆粕发酵物与天麻的混合物不仅能显著缩短小鼠睡眠潜伏期(P<0.05),还能显著延长小鼠睡眠时间(P<0.01),说明豆粕发酵物具有改善小鼠睡眠功效,并与天麻具有协同作用。
This paper studied the effect of improving slumber of soybean meal fermented product, gastrodiae and mixture of soybean meal fermented product and gastrodiae, this paper use ICR rats to conduct the experiment. Mice in soybean meal fermented product group were gavaged soybean meal fermented product and mice in gastrodiae group were gavaged gastrodiae, while mice in mixture group were gavaged mixture of soybean meal fermented product and gastrodiae.These three groups were all gavaged with the same dosage of 2.5 g/kg BW·d. Meanwhile, mice in control group were gavaged with same volume of distilled water. 14 days later, following experiments were conducted: direct hypnosis experiment; subthreshold dose of pentobarbital sodium hypnotic experiment; barbital sodium sleep latency experiment; prolonged pentobarbital sodium sleep time experiment. Meanwhile, the body weights and organ indexes of mice were recorded. According to the result of slumber test above, all the three test recipients can significantly improve mice sleep rate(P<0.05). Single soybean meal fermented product did not significantly reduce the sleep latency time while single gastrodiae did not significantly improve sleep time of mice. On contrary, the mixture of soybean meal fermented product and gastrodiae can both reduce the sleep latency time and improve sleep time of mice. The results above can demonstrate that soybean meal fermented product has an effect on improving slumber and it has a synergistic effect with gastrodiae.
This paper studied the effect of improving slumber of soybean meal fermented product, gastrodiae and mixture of soybean meal fermented product and gastrodiae, this paper use ICR rats to conduct the experiment. Mice in soybean meal fermented product group were gavaged soybean meal fermented product and mice in gastrodiae group were gavaged gastrodiae, while mice in mixture group were gavaged mixture of soybean meal fermented product and gastrodiae.These three groups were all gavaged with the same dosage of 2.5 g/kg BW·d. Meanwhile, mice in control group were gavaged with same volume of distilled water. 14 days later, following experiments were conducted: direct hypnosis experiment; subthreshold dose of pentobarbital sodium hypnotic experiment; barbital sodium sleep latency experiment; prolonged pentobarbital sodium sleep time experiment. Meanwhile, the body weights and organ indexes of mice were recorded. According to the result of slumber test above, all the three test recipients can significantly improve mice sleep rate(P<0.05). Single soybean meal fermented product did not significantly reduce the sleep latency time while single gastrodiae did not significantly improve sleep time of mice. On contrary, the mixture of soybean meal fermented product and gastrodiae can both reduce the sleep latency time and improve sleep time of mice. The results above can demonstrate that soybean meal fermented product has an effect on improving slumber and it has a synergistic effect with gastrodiae.
引文
[1]刘素杰,关乃鹏,闫海兵.大豆粕的营养价值及其品质评定[J].现代畜牧兽医, 2009, 10:30-32.
[2]P GARCIA-REBOLLAR, L CAMARA, R P LAZARO,et al. Influence of the origin of the beans on the chemical composition and nutritive value of commercial soybean meals[J]. Animal Feed Science and Technology, 2016, 221:245-261.
[3]曹颖霞,王思珍,武迎红,等.不同大豆粕(饼)样品粗蛋白质含量差异的分析[J].内蒙古民族大学学报(自然科学版), 2002, 17(3):240-241.
[4]张吉鹍.肽营养及其在开发大豆肽饲料中的应用[J].饲料与畜牧, 2010,(1):22-25.
[5]张晓峥,刑蓬蕊,张显香,等.发酵豆粕中大豆异黄酮的提取及含量测定[J].泰山医学院学报, 2015,36(1):57-60.
[6]吴永,沛刘明,倪辉.大豆抗氧化活性肽固态发酵工艺优化[J].中国食品学报, 2007, 7(6):52-57.
[7]刘海燕,闫晓刚,邱玉朗,等.发酵酶解豆粕对小鼠血液抗氧化指标和胃肠道消化酶活性及肠道菌群的影响[J].营养饲料, 2011, 47(21):41-44.
[8]徐聪聪.固态发酵豆粕制备大豆抗氧化活性肽的研究[D].广州:暨南大学, 2013.
[9]XUEJUAN Y, JUN C, CHUNXIAO Z, et al. Evaluation of antioxidant activity of fermented soybean meal extract[J]. African Journal of Pharmacy and Pharmacology, 2012, 6(24):1774-1781.
[10]NAIFU W, GUOWEI L, YONGHUI S, et al. Production of Bioactive peptides from soybean meal by solid state fermentation with lactic acid bacteria and protease[J]. Advanced Journal of Food Sciencce and Techonlogy, 2014, 6(9):1080-1085.
[11]HAIKUAN W, SHANTING Z, YAN S, et al.ACE-inhibitory peptide isolated from fermented soybean meal as functional food[J]. International Journal of Food Engineering, 2013, 9(1):1-8.
[12]熊莹,吴先华,覃小荣,等.发酵豆粕对保育猪生长性能及免疫力的影响研究[J].单胃动物营养,2016,(7):23-26.
[13]BARNES M E, BROWN M L, BRUCE T J, et al.Effects of a fermented soybean meal diet on rainbow trout mortality and immune function during a disease outbreak[J]. Journal of Aquaculture Feed Science and Nutrition, 2015, 7(1/2/3):6-15.
[14]TANG J W, SUN H, YAO X H, et al. Effects of replacement of soybean meal by fermented cottonseed meal on growth performance, serum biochemical parameters and immune function of yellow-feathered broilers[J]. Asian-Australasian Journal of Animal Sciences, 2012, 25(3):393-400.
[15]WANG N F, CHEN Q. Effect of lactic acid fermented soybean meal on the grow the performance intestinal microflora and morphology of weaned piglets[J]. Journal of Animal and Feed Sciences, 2007,(16):75-85.
[16]许丽惠,祁瑞雪.发酵豆粕对黄羽肉鸡生长性能、血清生化指标、肠道黏膜免疫功能及微生物菌群的影响[J].动物营养学报, 2013, 25(4):840-848.
[17]李云兰,高启平,帅柯,等.发酵豆粕替代豆粕对鲤鱼生长性能和肠道组织结构的影响[J].动物营养学报, 2015, 27(2):469-475.
[18]傅惠英,寿旗扬,李艳伟,等.中国被毛孢发酵物对睡眠剥夺亚健康大鼠的影响[J].中国实验方剂学杂志, 2009, 15(6):59-62.
[19]黄维,谷燕,刘燕,等.裂褶菌发酵酸枣仁产物改善睡眠的研究进展[J].科技信息, 2011,(1):18.
[20]李春莉.王浆天麻复方改善睡眠的功能研究[D].福州:福建农林大学, 2009.
[21]YANG Z, XIAOJIAN G, XIN Z, et al. Relative bioavailability of gastrodin and parishin from extract and powder of gastrodiae rhizome in rat[J]. Journal of Pharmaceutical and Biomedical Analysis, 2014,100:309-315.
[22]黄彬,石京山.天麻对大鼠脑内多巴胺含量及释放的影响[J].贵州医药, 1993, 17(1):35-35.
[23]孙亚南.天麻保健功能研究进展[J].中国老年学杂志, 2009, 29(7):899-901.
[24]罗娟.超声对枯草芽孢杆菌液态发酵豆粕及其产物功能特性的影响[D].镇江:江苏大学, 2016.
[25]黄雨三.保健食品检验与评价技术规范实施手册[M].北京:清华同方电子出版社, 2003:81-83.
[26]齐丽娟,高珊,马玲,等.天麻改善睡眠工程的实验研究[J].首都公共卫生, 2012, 6(2):66-68.
[27]蒲昭和.有关天麻毒副作用的临床报道及认识[J].中国中医药信息杂志, 1991, 4(3):12-13.
[2]P GARCIA-REBOLLAR, L CAMARA, R P LAZARO,et al. Influence of the origin of the beans on the chemical composition and nutritive value of commercial soybean meals[J]. Animal Feed Science and Technology, 2016, 221:245-261.
[3]曹颖霞,王思珍,武迎红,等.不同大豆粕(饼)样品粗蛋白质含量差异的分析[J].内蒙古民族大学学报(自然科学版), 2002, 17(3):240-241.
[4]张吉鹍.肽营养及其在开发大豆肽饲料中的应用[J].饲料与畜牧, 2010,(1):22-25.
[5]张晓峥,刑蓬蕊,张显香,等.发酵豆粕中大豆异黄酮的提取及含量测定[J].泰山医学院学报, 2015,36(1):57-60.
[6]吴永,沛刘明,倪辉.大豆抗氧化活性肽固态发酵工艺优化[J].中国食品学报, 2007, 7(6):52-57.
[7]刘海燕,闫晓刚,邱玉朗,等.发酵酶解豆粕对小鼠血液抗氧化指标和胃肠道消化酶活性及肠道菌群的影响[J].营养饲料, 2011, 47(21):41-44.
[8]徐聪聪.固态发酵豆粕制备大豆抗氧化活性肽的研究[D].广州:暨南大学, 2013.
[9]XUEJUAN Y, JUN C, CHUNXIAO Z, et al. Evaluation of antioxidant activity of fermented soybean meal extract[J]. African Journal of Pharmacy and Pharmacology, 2012, 6(24):1774-1781.
[10]NAIFU W, GUOWEI L, YONGHUI S, et al. Production of Bioactive peptides from soybean meal by solid state fermentation with lactic acid bacteria and protease[J]. Advanced Journal of Food Sciencce and Techonlogy, 2014, 6(9):1080-1085.
[11]HAIKUAN W, SHANTING Z, YAN S, et al.ACE-inhibitory peptide isolated from fermented soybean meal as functional food[J]. International Journal of Food Engineering, 2013, 9(1):1-8.
[12]熊莹,吴先华,覃小荣,等.发酵豆粕对保育猪生长性能及免疫力的影响研究[J].单胃动物营养,2016,(7):23-26.
[13]BARNES M E, BROWN M L, BRUCE T J, et al.Effects of a fermented soybean meal diet on rainbow trout mortality and immune function during a disease outbreak[J]. Journal of Aquaculture Feed Science and Nutrition, 2015, 7(1/2/3):6-15.
[14]TANG J W, SUN H, YAO X H, et al. Effects of replacement of soybean meal by fermented cottonseed meal on growth performance, serum biochemical parameters and immune function of yellow-feathered broilers[J]. Asian-Australasian Journal of Animal Sciences, 2012, 25(3):393-400.
[15]WANG N F, CHEN Q. Effect of lactic acid fermented soybean meal on the grow the performance intestinal microflora and morphology of weaned piglets[J]. Journal of Animal and Feed Sciences, 2007,(16):75-85.
[16]许丽惠,祁瑞雪.发酵豆粕对黄羽肉鸡生长性能、血清生化指标、肠道黏膜免疫功能及微生物菌群的影响[J].动物营养学报, 2013, 25(4):840-848.
[17]李云兰,高启平,帅柯,等.发酵豆粕替代豆粕对鲤鱼生长性能和肠道组织结构的影响[J].动物营养学报, 2015, 27(2):469-475.
[18]傅惠英,寿旗扬,李艳伟,等.中国被毛孢发酵物对睡眠剥夺亚健康大鼠的影响[J].中国实验方剂学杂志, 2009, 15(6):59-62.
[19]黄维,谷燕,刘燕,等.裂褶菌发酵酸枣仁产物改善睡眠的研究进展[J].科技信息, 2011,(1):18.
[20]李春莉.王浆天麻复方改善睡眠的功能研究[D].福州:福建农林大学, 2009.
[21]YANG Z, XIAOJIAN G, XIN Z, et al. Relative bioavailability of gastrodin and parishin from extract and powder of gastrodiae rhizome in rat[J]. Journal of Pharmaceutical and Biomedical Analysis, 2014,100:309-315.
[22]黄彬,石京山.天麻对大鼠脑内多巴胺含量及释放的影响[J].贵州医药, 1993, 17(1):35-35.
[23]孙亚南.天麻保健功能研究进展[J].中国老年学杂志, 2009, 29(7):899-901.
[24]罗娟.超声对枯草芽孢杆菌液态发酵豆粕及其产物功能特性的影响[D].镇江:江苏大学, 2016.
[25]黄雨三.保健食品检验与评价技术规范实施手册[M].北京:清华同方电子出版社, 2003:81-83.
[26]齐丽娟,高珊,马玲,等.天麻改善睡眠工程的实验研究[J].首都公共卫生, 2012, 6(2):66-68.
[27]蒲昭和.有关天麻毒副作用的临床报道及认识[J].中国中医药信息杂志, 1991, 4(3):12-13.