正交实验法优选香加皮中总黄酮的提取工艺
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摘要
通过正交实验法,优选香加皮药材中总黄酮的最佳提取工艺。以香加皮中总黄酮的提取率作为指标,采用超声辅助法对香加皮药材中总黄酮进行提取,以乙醇浓度(A)、超声时间(B)、温度(C)三个提取条件进行单因素实验,在单因素实验的基础上,设计三因素三水平的正交试验。对香加皮中总黄酮提取影响最大的因素是温度,超声辅助法提取香加皮总黄酮的最佳条件为:乙醇浓度90%,温度60℃,超声时间为35 min,药渣重复提取一次。在此工艺条件下香加皮中总黄酮的提取率为2.313%。用超声辅助法进行香加皮总黄酮提取操作简便、提取率高,此数据结果可为香加皮的进一步研究提供理论依据。
The optimal extraction process of total flavonoids from cortex periplocae was preferred by orthogonal experiment. The extraction ratio of total flavonoids from cortex periplocae was considered as index, the flavonoids were extracted by ultrasonic assisted method. Single factor experiment with ethanol concentration(A), ultrasonic time(B) and temperature(C) as three conditions was carried out. Based on the single factor experiment, the orthogonal experiment of three factors and three levels was designed. The results showed that the most important factor affecting the extraction of total flavonoids from cortex periplocae was the temperature. The optimal conditions for the extraction of total flavonoids from cortex periplocae by ultrasonic assisted method were as follows: the ethanol concentration90%, the temperature 60 ℃,the ultrasonic time 35 min. The dregs were extracted again. Under above condition, the extraction rate of total flavonoids from cortex periplocae was 2.313%. Ultrasound-assisted extraction of total flavonoids from cortex periplocae is simple and efficient. The results of this data can provide theoretical basis for the further study of cortex periplocae.
The optimal extraction process of total flavonoids from cortex periplocae was preferred by orthogonal experiment. The extraction ratio of total flavonoids from cortex periplocae was considered as index, the flavonoids were extracted by ultrasonic assisted method. Single factor experiment with ethanol concentration(A), ultrasonic time(B) and temperature(C) as three conditions was carried out. Based on the single factor experiment, the orthogonal experiment of three factors and three levels was designed. The results showed that the most important factor affecting the extraction of total flavonoids from cortex periplocae was the temperature. The optimal conditions for the extraction of total flavonoids from cortex periplocae by ultrasonic assisted method were as follows: the ethanol concentration90%, the temperature 60 ℃,the ultrasonic time 35 min. The dregs were extracted again. Under above condition, the extraction rate of total flavonoids from cortex periplocae was 2.313%. Ultrasound-assisted extraction of total flavonoids from cortex periplocae is simple and efficient. The results of this data can provide theoretical basis for the further study of cortex periplocae.
引文
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[2]张翼飞,刘志凯,林雨晟,等.香加皮精多糖的单糖组成、热重和热裂解分析[J/OL].食品工业科技:1-11[2019-01-25].
[3]单保恩.中药香加皮抗肿瘤作用的研究[A].中国抗癌协会、中华医学会肿瘤学分会.第四届中国肿瘤学术大会暨第五届海峡两岸肿瘤学术会议论文集[C].中国抗癌协会、中华医学会肿瘤学分会:中国抗癌协会,2006:1.
[4]曹广超,王舒玉,王彦多,等.正交试验法优选发酵蜣螂粉中总黄酮的提取工艺[J].华西药学杂志,2018,33(03):305-308.
[5]路晓庆,杨芮,李炘正,等.黄酮类物质的生物功能及作用机制研究进展[J].中西医结合心脑血管病杂志,2018,16(22):3283-3286.
[6]麦合苏木·艾克木,努尔江·肉孜,阿不都热依木·玉苏甫.维药神香草总黄酮抗炎、止咳、祛痰及平喘作用研究[J].新疆医科大学学报,2014,37(04):403-406.
[7]李瑶佳,王君,毛攀,等.黄花石莲总黄酮超声辅助提取及抗氧化活性研究[J].食品工程,2018(04):37-42+48.
[8]吴保承,杨春霞,张栋,等.玉米须黄酮的超声波提取工艺研究[J].化学与生物工程,2011,28(07):67-69.
[9]陈红惠,李霖娜,聂秋月.广南底圩茶黄酮提取工艺研究[J].文山学院学报,2018,31(06):23-26.
[10]王彦多,刘春雨,吕世洁,等.正交实验法优化发酵蜈蚣粉中总黄酮提取工艺[J].食品工业科技,2017,38(07):183-186+192.
[11]李玲,陶亮,何香,等.明月草中黄酮提取工艺优化及抗氧化活性研究[J].食品与发酵科技,2018,54(06):61-65+86.
[12]马晓,陈刚,张洁.牡丹果皮黄酮工艺优化及抗氧化性研究[J].解放军药学学报,2018,34(06):506-509.
[13]鱼清雅,郭从嘉,毕进,等.正交试验法优选海南黎药冰糖草茎叶中总黄酮成分的提取工艺[J].陕西中医,2019,40(01):131-134.
[14]张国涛.超声波法提取荞麦粉中黄酮类化合物的工艺研究[J].当代化工,2017,46(08):1524-1526.
[15]许建本,苏秀芳,莫耀芳.超声波辅助法提取假苹婆树叶总黄酮及其清除羟自由基能力[J].食品工业科技,2018,39(23):199-202+209.
[16]陈燕媚,黄锁义.黄皮叶总黄酮的提取及鉴别[J].中国野生植物资源,2018,37(06):16-18.
[17]李瑞雪,张继全,吴飞,等.香加皮有效成分提取工艺和检测方法的研究进展[J].中国医药导报,2016,13(404):43-47.