丰富环境对单眼剥夺小鼠视皮质与外侧膝状体Sortilin表达的影响
|
摘要
目的:探讨丰富环境(EE)对弱视小鼠初级视皮质和外侧膝状体Sortilin的表达影响。方法:选择36只21 d昆明小鼠,雌雄不限,随机分为正常组(Control)、单眼剥夺+标准环境组(MD+SE)和单眼剥夺+丰富环境组(MD+EE),通过行为学检测各组小鼠视功能改变、免疫组织化学方法检测各组小鼠视皮质和外侧膝状体Sortilin的表达。结果:行为学结果显示:与Control组相比,MD+SE组探爪触地成功率明显降低(P <0. 05),MD+EE组探爪触地成功率显著高于MD+SE组(P <0. 05);免疫组织化学结果显示:与Control组相比,MD+SE组外侧膝状体背内侧Sortilin阳性神经元数目显著升高(P <0. 001); MD+EE组显著低于MD+SE组,但高于Control组(P <0. 001);与Control组相比,MD+SE组小鼠视皮质Sortilin阳性神经元数目显著降低(P <0. 001); MD+EE组显著高于MD+SE组(P <0. 001),但显著低于Control组(P <0. 001)。结论:丰富环境改善弱视小鼠视功能可能通过调节视皮质和外侧膝状体Sortilin的表达实现。
Objective: To investigate the effects of environmental enrichment( EE) on the expression of Sortilin in primary visual cortex and lateral geniculate body in amblyopia mice. Methods: 21 day old Kunming mice of eihher sex were randomly divided into Control group( CON),monocular deprivation + standard environment group( MD + SE) and monocular deprivation + environmental enrichment group( MD + EE),the visual function of each group was detected by behavioral analysis. The expression of Sortilin in the visual cortex and lateral geniculate body of each group was detected by immunohistochemistry. Results: The behavioral results showed that MD lead to significantly low success rate of the forepaw-reaching reflex in the visual placing test compared to the Control group( P < 0. 05),while EE significantly enhanced the success rate of the forepaw-reaching reflex compared to the MD + SE group( P < 0. 05). The immunohistochemical assay showed that there was statistically significant difference of the number of Sortilin positive cells in the dorsomedial side of thelateral geniculate body among the three groups( P < 0. 05). Compared with the Control group,the number of Sortilin positive cells was significantly increased in the MD + SE group( P < 0. 001),while EE significantly decreased the number of Sortilin positive cells compared to the MD + SE group,but enhanced compared to the Control group( P < 0. 001). There was statistically significant difference of the number of Sortilin positive cells in the visual cortex among the three groups( P <0. 05). Compared with the Control group,MD lead to significantly decreased number of Sortilin positive cells( P <0. 001),although EE significantly enhanced the number of Sortilin positive cells comapred to the MD + SE group,it was still reduced comapred to the Control group( P < 0. 001). Conclusion: Environmental enrichment improves visual function in amblyopic mice by regulating the expression of Sortilin in the visual cortex and lateral geniculate body.
Objective: To investigate the effects of environmental enrichment( EE) on the expression of Sortilin in primary visual cortex and lateral geniculate body in amblyopia mice. Methods: 21 day old Kunming mice of eihher sex were randomly divided into Control group( CON),monocular deprivation + standard environment group( MD + SE) and monocular deprivation + environmental enrichment group( MD + EE),the visual function of each group was detected by behavioral analysis. The expression of Sortilin in the visual cortex and lateral geniculate body of each group was detected by immunohistochemistry. Results: The behavioral results showed that MD lead to significantly low success rate of the forepaw-reaching reflex in the visual placing test compared to the Control group( P < 0. 05),while EE significantly enhanced the success rate of the forepaw-reaching reflex compared to the MD + SE group( P < 0. 05). The immunohistochemical assay showed that there was statistically significant difference of the number of Sortilin positive cells in the dorsomedial side of thelateral geniculate body among the three groups( P < 0. 05). Compared with the Control group,the number of Sortilin positive cells was significantly increased in the MD + SE group( P < 0. 001),while EE significantly decreased the number of Sortilin positive cells compared to the MD + SE group,but enhanced compared to the Control group( P < 0. 001). There was statistically significant difference of the number of Sortilin positive cells in the visual cortex among the three groups( P <0. 05). Compared with the Control group,MD lead to significantly decreased number of Sortilin positive cells( P <0. 001),although EE significantly enhanced the number of Sortilin positive cells comapred to the MD + SE group,it was still reduced comapred to the Control group( P < 0. 001). Conclusion: Environmental enrichment improves visual function in amblyopic mice by regulating the expression of Sortilin in the visual cortex and lateral geniculate body.
引文
[1]Dolzhich AV,Avetisov SE.Clinical effectiveness study of transcranial direct current stimulation in amblyopic children[J].Vestn Oftalmol,2018,134:38-42.
[2]Siu C,Murphy K.The development of human visual cortex and clinical implications[J].Eye&Brain,2018,10:25-36.
[3]Cao ZX,Liu AG,Zhu TT,et al.Molecular biology of the treatment of amblyopia with acupuncture:a review of recent research[J].针制研究,2018,43:189-193.
[4]Bonaccorsi J,Berardi N,Sale A.Treatment of amblyopia in the adult:insights from a new rodent model of visual perceptual learning[J].Front Neural Circuits,2014,8:82.
[5]王文宏,刘相和,迟焕芳.枸杞子提取液对单眼视觉剥夺性弱视大鼠视网膜的保护作用[J].神经解剖学杂志,2010,26:268-272.
[6]Sale A,Berardi N.Active training for amblyopia in adult rodents[J].Front Behav Neurosci,2015,9:10960-10967.
[7]Zhou Y,Lai B,Gan W.Monocular deprivation induces dendritic spine elimination in the developing mouse visual cortex[J].Sci Rep,2017,7:4977.
[8]胡招兰,曹文宇,王雪琴,等.Pro-BDNF及其受体在实验性变态反应性脑脊髓炎小鼠中枢神经系统的表达变化[J].神经解剖学杂志,2015,31:1-7.
[9]Bai YY,Ruan CS,Yang CR,et al.Pro BDNF signaling regulates depression-like behaviors in rodents under chronic stress[J].Neuropsychopharmacology,2016,41:2882-2892.
[10]Guirado R,La Terra D,Bourguignon M,et al.Effects of PSA removal from NCAM on the critical period plasticity triggered by the antidepressant fluoxetine in the visual cortex[J].Front Cell Neurosci,2016,10:22.
[11]Cao WY,Hu ZL,Xu Y,et al.Role of early environmental enrichment on the social dominance tube test at adulthood in the rat[J].Psychopharmacology(Berl),2017,234:3321-3334.
[12]Dolzhich AV,Avetisov SE.Clinical effectiveness study of transcranial direct current stimulation in amblyopic children[J].Vestn Oftalmol,2018,134:38-42.
[13]Ding K,Liu Y,Yan X,et al.Altered functional connectivity of the primary visual cortex in subjects with amblyopia[J].Neural Plast,2013,2013:612086.
[14]Baroncelli L,Bonaccorsi J,Milanese M,et al.Enriched experience and recovery from amblyopia in adult rats:impact of motor,social and sensory components[J].Neuropharmacology,2012,62:2388-2397.
[15]Baroncelli L,Cenni MC,Melani R,et al.Early IGF-1 primes visual cortex maturation and accelerates developmental switch between NKCC1 and KCC2 chloride transporters in enriched animals[J].Neuropharmacology,2016,113:167-177.
[16]Crewther DP,Crewther SG.A new model of strabismic amblyopia:Loss of spatial acuity due to increased temporal dispersion of geniculate X-cell afferents on to cortical neurons[J].Vision Res,2015,114:79-86.
[17]Sloper J.The other side of amblyopia[J].J AAPOS,2016,20:1.
[18]Fong MF,Mitchell DE,Duffy KR.Rapid recovery from the effects of early monocular deprivation is enabled by temporary inactivation of the retinas[J].Proc Natl Acad Sci USA,2016,113:14139-14144.
[19]李花,徐杨,曹文宇,等.炎性痛致大鼠脊髓后角Pro BD-NF及其受体的表达变化[J].神经解剖学杂志,2011,27:365-370.
[20]Rahman M,Luo H,Sims NR,et al.Investigation of mature BDNFand pro BDNF signaling in a rat photothrombotic ischemic model[J].Neurochem Res,2018,43:637-649.
[2]Siu C,Murphy K.The development of human visual cortex and clinical implications[J].Eye&Brain,2018,10:25-36.
[3]Cao ZX,Liu AG,Zhu TT,et al.Molecular biology of the treatment of amblyopia with acupuncture:a review of recent research[J].针制研究,2018,43:189-193.
[4]Bonaccorsi J,Berardi N,Sale A.Treatment of amblyopia in the adult:insights from a new rodent model of visual perceptual learning[J].Front Neural Circuits,2014,8:82.
[5]王文宏,刘相和,迟焕芳.枸杞子提取液对单眼视觉剥夺性弱视大鼠视网膜的保护作用[J].神经解剖学杂志,2010,26:268-272.
[6]Sale A,Berardi N.Active training for amblyopia in adult rodents[J].Front Behav Neurosci,2015,9:10960-10967.
[7]Zhou Y,Lai B,Gan W.Monocular deprivation induces dendritic spine elimination in the developing mouse visual cortex[J].Sci Rep,2017,7:4977.
[8]胡招兰,曹文宇,王雪琴,等.Pro-BDNF及其受体在实验性变态反应性脑脊髓炎小鼠中枢神经系统的表达变化[J].神经解剖学杂志,2015,31:1-7.
[9]Bai YY,Ruan CS,Yang CR,et al.Pro BDNF signaling regulates depression-like behaviors in rodents under chronic stress[J].Neuropsychopharmacology,2016,41:2882-2892.
[10]Guirado R,La Terra D,Bourguignon M,et al.Effects of PSA removal from NCAM on the critical period plasticity triggered by the antidepressant fluoxetine in the visual cortex[J].Front Cell Neurosci,2016,10:22.
[11]Cao WY,Hu ZL,Xu Y,et al.Role of early environmental enrichment on the social dominance tube test at adulthood in the rat[J].Psychopharmacology(Berl),2017,234:3321-3334.
[12]Dolzhich AV,Avetisov SE.Clinical effectiveness study of transcranial direct current stimulation in amblyopic children[J].Vestn Oftalmol,2018,134:38-42.
[13]Ding K,Liu Y,Yan X,et al.Altered functional connectivity of the primary visual cortex in subjects with amblyopia[J].Neural Plast,2013,2013:612086.
[14]Baroncelli L,Bonaccorsi J,Milanese M,et al.Enriched experience and recovery from amblyopia in adult rats:impact of motor,social and sensory components[J].Neuropharmacology,2012,62:2388-2397.
[15]Baroncelli L,Cenni MC,Melani R,et al.Early IGF-1 primes visual cortex maturation and accelerates developmental switch between NKCC1 and KCC2 chloride transporters in enriched animals[J].Neuropharmacology,2016,113:167-177.
[16]Crewther DP,Crewther SG.A new model of strabismic amblyopia:Loss of spatial acuity due to increased temporal dispersion of geniculate X-cell afferents on to cortical neurons[J].Vision Res,2015,114:79-86.
[17]Sloper J.The other side of amblyopia[J].J AAPOS,2016,20:1.
[18]Fong MF,Mitchell DE,Duffy KR.Rapid recovery from the effects of early monocular deprivation is enabled by temporary inactivation of the retinas[J].Proc Natl Acad Sci USA,2016,113:14139-14144.
[19]李花,徐杨,曹文宇,等.炎性痛致大鼠脊髓后角Pro BD-NF及其受体的表达变化[J].神经解剖学杂志,2011,27:365-370.
[20]Rahman M,Luo H,Sims NR,et al.Investigation of mature BDNFand pro BDNF signaling in a rat photothrombotic ischemic model[J].Neurochem Res,2018,43:637-649.