α-半乳糖苷酶及其在饲料中的应用
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摘要
半乳糖苷酶是催化α-半乳糖苷键水解的一种外切糖苷酶,可以催化水解棉子糖、水苏糖、毛蕊花糖及其衍生物,在自然界中分布广泛。本文主要对其来源与分类、酶学特性、结构与催化特点及其在饲料中的应用进行了阐述,以期促进该酶的进一步推广和利用。
galactosidase is an exo-glycosidase that catalyzes the hydrolysis of α-galactosidase. It can catalyze the hydrolysis of cottonose,stachyose,ruffin and its derivatives,which is widely distributed in nature. In this paper,its origin and classification,enzymatic characteristics,structure and catalytic characteristics and its application in feed industry were described,and its role as a new enzyme preparation in feed was revealed,which was expected to promote the further promotion and utilization of the enzyme.
galactosidase is an exo-glycosidase that catalyzes the hydrolysis of α-galactosidase. It can catalyze the hydrolysis of cottonose,stachyose,ruffin and its derivatives,which is widely distributed in nature. In this paper,its origin and classification,enzymatic characteristics,structure and catalytic characteristics and its application in feed industry were described,and its role as a new enzyme preparation in feed was revealed,which was expected to promote the further promotion and utilization of the enzyme.
引文
[1]戴求伸,张民.日粮中添加α-半乳糖苷酶对黄羽肉鸡生产性能的影响及相关机理研究[J].饲料工业,2010,31(6):19~25.
[2]蒋小丰,戴求仲,蒋桂韬,等.α-半乳糖苷酶对断奶仔猪生产性能、血液生化指标和营养物质消化率的影响[J].家畜生态学报,2009,30(6):44~49.
[3]马慧慧,李绍钰.α-半乳糖苷酶对AA肉鸡生长性能和血清生化指标的影响[J].中国兽医学报,2013,33(7):1059~1063.
[4]缪志军,王向荣,蒋桂韬,等.复合非淀粉多糖酶制剂中补充α-半乳糖苷酶对番鸭生产性能的影响[J].中国饲料,2010,12:17~19.
[5] Baucells F,Perez P F,Morales J,et al.Effect of alpha-galactosidase supplementation of cereal-soya-bean-pea diets on the productive performances,digestibility and lower gut fermentation in growing and finishing pigs[J].Animal Science,2016,71(1):157~164.
[6] Brouns S J J,Smits N,Wu H,et al.Identification of a Novelα-Galactosidase from the Hyperthermophilic Archaeon Sulfolobus solfataricus[J].Journal of Bacteriology 2006,188(7):2392.
[7] Cao Y N,Yuan T Z,Shi P J,et al.Properties of a novelα-galactosidase from Streptomyces sp.S27 and its potential for soybean processing[J].Enzyme&Microbial Technology,2010,47(7):305~312.
[8] Carrerasilva E A,Silvestroni A,Leblanc J G,et al.A thermostable alpha-galactosidase from Lactobacillus fermentum CRL722:genetic characterization and main properties[J].Current Microbiology,2006,53(5):374~378.
[9] Chen Z,Yan Q,Jiang Z,et al.High-level expression of a novelα-galactosidase gene from Rhizomucor miehei in Pichia pastoris and characterization of the recombinant enyzme[J].Protein Expression&Purification,2015,110:107~114.
[10] Coon C N,Leske K L,Akavanichan O,et al.Effect of Oligosaccharide-Free Soybean Meal on True Metabolizable Energy and Fiber Digestion in Adult Roosters[J].Poultry Science,1990,69(5):787~793.
[11] Elshafei A M,Foda M S,Abdel-Mobde E,et al.Optimization of alpha-galactosidase production in Streptomyces erythrus[J].Acta Microbiol Pol,2001,50(1):53~63.
[12] Fredslund F,Hachem M A,Larsen R J,et al.Crystal Structure ofα-Galactosidase from Lactobacillus acidophilus NCFM:Insight into Tetramer Formation and Substrate Binding[J].Journal of Molecular Biology,2011,412(3):466~480.
[13] Fridjonsson O,Watzlawick H,Gehweiler A,et al.Thermostable alpha-galactosidase from Bacillus stearothermophilus NUB3621:cloning,sequencing and characterization[J].Fems Microbiology Letters,1999,176(1):147~153.
[14] Fridjonsson O,Watzlawick H,Gehweiler A,et al.Cloning of the Gene Encoding a Novel Thermostableα-Galactosidase from Thermus brockianus ITI360[J].Applied&Environmental Microbiology,1999,65(9):3955~3963.
[15] Fridjonsson O,Mattes R.Production of recombinant alphagalactosidases in Thermus thermophilus[J].Applied&Environmental Microbiology,2001,67(9):4192~4198.
[16] Fujimoto Z,Kaneko S,Momma M,et al.Crystal Structure of Riceα-Galactosidase Complexed with D-Galactose[J].Journal of Biological Chemistry,2003,278.
[17] Ghazi S,Rooke J A,Galbraith H,et al.Improvement of the nutritive value of soybean meal by protease and a-galactosidase treatment in broiler cockerels and broiler chicks[J].British Poultry Science,2003,44(3):410~418.
[18] Gherardini F,Babcock M,Salyers A A,et al.Purification and characterization of two alpha-galactosidases associated with catabolism of guar gum and other alpha-galactosides by Bacteroides ovatus[J].Journal of Bacteriology,1985,161(2):500~506.
[19] Gloster T M,Turkenburg J P,Potts J R,et al.Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora[J].Chemistry&Biology,2008,15(10):1058~1067.
[20] Gomes J,Gomes II,Terler K,et al.Optimisation of culture medium and conditions for alpha-l-Arabinofuranosidase production by the extreme thermophilic eubacterium Rhodothermus marinus[J].Enzyme&Microbial Technology,2000,27(6):414~422.
[21] Gote M M,Khan M I,Gokhale D V,et al.Purification,characterization and substrate specificity of thermostable a-galactosidase from Bacillus stearothermophilus[J].Process Biochemistry,2006,41(6):1311~1317.
[22] Gurkok S,Cekmecelioglu D,Ogel Z B,et al.Optimization of culture conditions for Aspergillus sojae expressing an Aspergillus fumigatus a-galactosidase[J].Bioresource Technology,2011,102(7):4925~4929.
[23] Han B Z,Ma Y,Rombouts F M,et al.Effects of temperature and relative humidity on growth and enzyme production by Actinomucor elegans and Rhizopus oligosporus during sufu pehtze preparation[J].Food Chemistry,2003,81(1):27~34.
[24] Kim D S,Cho P S,Lee J H,et al.Alpha-galactosidase of Bifidobacterium adolescentis DSM 20083[J].Current Microbiology,1999,38(2):101~106.
[25] Kuo T M,Vanmiddlesworth J F,Wolf W J,et al.Content of raffinose oligosaccharides and sucrose in various plant seeds[J].J agric food Chem,1988,36(1):32~36.
[26] Lallés J P.Nutritional and antinutritional aspects of soyabean and field pea proteins used in veal calf production:a review[J].Livestock Production Science,1993,34(3~4):181~202.
[27] Lieshout JFTV,Verhees C H,Ettema T J G,et al.Identification and Molecular Characterization of a Novel Type of IⅠ±-galactosidase from Pyrococcus furiosus[J].Biocatalysis,2009,21(4~5):243~252.
[28] Luonteri E,Alatalo E,Siikaaho M,et al.alpha-Galactosidases of Penicillium simplicissimum:production,purification and characterization of the gene encoding AGLI[J].Biotechnology&Applied Biochemistry,1998,28(2):179~188.
[29] Muli mani V H,Ramalingam.Enzymic hydrolysis of raffinose and stachyose in soymilk by alpha-galactosidase from Gibberella fujikuroi[J].Biochemistry&Molecular Biology International,1995,36(4):897~905.
[30] Naumov D G.Phylogenetic analysis of a-galactosidases of the GH27 family[J].Molekuliarnaia Biologiia,2004,38(3):388~400.
[31] Okuyama M,Kitamura M,Hondoh H,et al.Catalytic mechanism of retaining alpha-galactosidase belonging to glycoside hydrolase family97[J].Journal of Molecular Biology,2009,392(5):1232~1241.
[32] Post D A,Luebke V E.Purification,cloning,and properties of al pha-galactosidase from Saccharopolyspora erythraea and its use as a reporter system[J].Applied Microbiology&Biotechnology,2005,67(1):91.
[33] Puchart A V,Vrsanska A M,Bhat B M K,et al.Purification and characterization ofα-galactosidase from a thermophilic fungus Thermomyces lanuginosus[J].Biochim Biophys Acta,2000,1524(1):27~37.
[34] Rezessyszab J M,Nguyen Q D,Hoschke A,et al.A novel thermostable alpha-galactosidase from the thermophilic fungus Thermomyces lanuginosus CBS 395.62/b:purification and characterization[J].Biochimica Et Biophysica Acta General Subjects,2007,1770(1):55~62.
[35] Shankar S K,Mulimani V H.Alpha-galactosidase production by Aspergillus oryzae in solid-state fermentation[J].Bioresource Technology,2007,98(4):958~961.
[36] Suresh C,Rus'd A A,Kitaokaa~M,et al.Evidence that the putative K-glucosidase of Thermotoga maritima MSB8 is a pNP KD-glucuronopyranoside hydrolyzing K-glucuronidase[J].FEBS Letters,2002,517(1~3):159~162.
[37] Suzuki H,Li S C,Li Y T,et al.Alpha-galactosidase from Mortierella vinacea.Crystallization and properties[J].Journal of Biological Chemistry,1970,245(4):781~786.
[38] Svastits-Dücs.o.Lilla,Nguyen Q D,Lefler D D,et al.Effects of galactomannan as carbon source on production ofα-galactosidase by Thermomyces lanuginosus:Fermentation,purification and partial characterization[J].Enzyme&Microbial Technology,2009,45(5):367~371.
[39] Thompson J,Pikis A,Ruvinov S B,et al.The gene glvA of Bacillus subtilis 168 encodes a metal-requiring,NAD(H)-dependent 6-phospho-alpha-glucosidase.Assignment to family 4 of the glycosylhydrolase superfamily[J].Journal of Biological Chemistry,1998,273(42):27347~27356.
[40] Ulezlo I V,Zaprometova O M.Microbial alpha-galactosidase(a review)[J].Prikladnaia Biokhimiia I Mikrobiologiia,1982,18(1):3.
[41] Vries R P D,Broeck H C V D,Dekkers E,et al.Differential Expression of Threeα-Galactosidase Genes and a Singleβ-Galactosidase Gene from Aspergillus niger[J].Applied&Environmental Microbiology,1999,65(6):2453.
[42] Wang H,Shi P,Luo H,et al.A thermophilicα-galactosidase from Neosartorya fischeri P1 with high specific activity,broad substrate specificity and significant hydrolysis ability of soymilk[J].Bioresource Technology,2014,153:361~364.
[43] Xiao M,Qian X Y K,Kumagai H,et al.High-yield production and characterization of alpha-galactosidase from Bifidobacterium breve grown on raffinose[J].Biotechnology Letters,2000,22(9):747~751.
[44] Zapater I G.Extracellular alpha galactosidase(E.C.3.2.1.22)from Aspergillus ficuum NRRL 3135 purification and characterization[J].Preparative Biochemistry,1990,20(3~4):263.
[2]蒋小丰,戴求仲,蒋桂韬,等.α-半乳糖苷酶对断奶仔猪生产性能、血液生化指标和营养物质消化率的影响[J].家畜生态学报,2009,30(6):44~49.
[3]马慧慧,李绍钰.α-半乳糖苷酶对AA肉鸡生长性能和血清生化指标的影响[J].中国兽医学报,2013,33(7):1059~1063.
[4]缪志军,王向荣,蒋桂韬,等.复合非淀粉多糖酶制剂中补充α-半乳糖苷酶对番鸭生产性能的影响[J].中国饲料,2010,12:17~19.
[5] Baucells F,Perez P F,Morales J,et al.Effect of alpha-galactosidase supplementation of cereal-soya-bean-pea diets on the productive performances,digestibility and lower gut fermentation in growing and finishing pigs[J].Animal Science,2016,71(1):157~164.
[6] Brouns S J J,Smits N,Wu H,et al.Identification of a Novelα-Galactosidase from the Hyperthermophilic Archaeon Sulfolobus solfataricus[J].Journal of Bacteriology 2006,188(7):2392.
[7] Cao Y N,Yuan T Z,Shi P J,et al.Properties of a novelα-galactosidase from Streptomyces sp.S27 and its potential for soybean processing[J].Enzyme&Microbial Technology,2010,47(7):305~312.
[8] Carrerasilva E A,Silvestroni A,Leblanc J G,et al.A thermostable alpha-galactosidase from Lactobacillus fermentum CRL722:genetic characterization and main properties[J].Current Microbiology,2006,53(5):374~378.
[9] Chen Z,Yan Q,Jiang Z,et al.High-level expression of a novelα-galactosidase gene from Rhizomucor miehei in Pichia pastoris and characterization of the recombinant enyzme[J].Protein Expression&Purification,2015,110:107~114.
[10] Coon C N,Leske K L,Akavanichan O,et al.Effect of Oligosaccharide-Free Soybean Meal on True Metabolizable Energy and Fiber Digestion in Adult Roosters[J].Poultry Science,1990,69(5):787~793.
[11] Elshafei A M,Foda M S,Abdel-Mobde E,et al.Optimization of alpha-galactosidase production in Streptomyces erythrus[J].Acta Microbiol Pol,2001,50(1):53~63.
[12] Fredslund F,Hachem M A,Larsen R J,et al.Crystal Structure ofα-Galactosidase from Lactobacillus acidophilus NCFM:Insight into Tetramer Formation and Substrate Binding[J].Journal of Molecular Biology,2011,412(3):466~480.
[13] Fridjonsson O,Watzlawick H,Gehweiler A,et al.Thermostable alpha-galactosidase from Bacillus stearothermophilus NUB3621:cloning,sequencing and characterization[J].Fems Microbiology Letters,1999,176(1):147~153.
[14] Fridjonsson O,Watzlawick H,Gehweiler A,et al.Cloning of the Gene Encoding a Novel Thermostableα-Galactosidase from Thermus brockianus ITI360[J].Applied&Environmental Microbiology,1999,65(9):3955~3963.
[15] Fridjonsson O,Mattes R.Production of recombinant alphagalactosidases in Thermus thermophilus[J].Applied&Environmental Microbiology,2001,67(9):4192~4198.
[16] Fujimoto Z,Kaneko S,Momma M,et al.Crystal Structure of Riceα-Galactosidase Complexed with D-Galactose[J].Journal of Biological Chemistry,2003,278.
[17] Ghazi S,Rooke J A,Galbraith H,et al.Improvement of the nutritive value of soybean meal by protease and a-galactosidase treatment in broiler cockerels and broiler chicks[J].British Poultry Science,2003,44(3):410~418.
[18] Gherardini F,Babcock M,Salyers A A,et al.Purification and characterization of two alpha-galactosidases associated with catabolism of guar gum and other alpha-galactosides by Bacteroides ovatus[J].Journal of Bacteriology,1985,161(2):500~506.
[19] Gloster T M,Turkenburg J P,Potts J R,et al.Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora[J].Chemistry&Biology,2008,15(10):1058~1067.
[20] Gomes J,Gomes II,Terler K,et al.Optimisation of culture medium and conditions for alpha-l-Arabinofuranosidase production by the extreme thermophilic eubacterium Rhodothermus marinus[J].Enzyme&Microbial Technology,2000,27(6):414~422.
[21] Gote M M,Khan M I,Gokhale D V,et al.Purification,characterization and substrate specificity of thermostable a-galactosidase from Bacillus stearothermophilus[J].Process Biochemistry,2006,41(6):1311~1317.
[22] Gurkok S,Cekmecelioglu D,Ogel Z B,et al.Optimization of culture conditions for Aspergillus sojae expressing an Aspergillus fumigatus a-galactosidase[J].Bioresource Technology,2011,102(7):4925~4929.
[23] Han B Z,Ma Y,Rombouts F M,et al.Effects of temperature and relative humidity on growth and enzyme production by Actinomucor elegans and Rhizopus oligosporus during sufu pehtze preparation[J].Food Chemistry,2003,81(1):27~34.
[24] Kim D S,Cho P S,Lee J H,et al.Alpha-galactosidase of Bifidobacterium adolescentis DSM 20083[J].Current Microbiology,1999,38(2):101~106.
[25] Kuo T M,Vanmiddlesworth J F,Wolf W J,et al.Content of raffinose oligosaccharides and sucrose in various plant seeds[J].J agric food Chem,1988,36(1):32~36.
[26] Lallés J P.Nutritional and antinutritional aspects of soyabean and field pea proteins used in veal calf production:a review[J].Livestock Production Science,1993,34(3~4):181~202.
[27] Lieshout JFTV,Verhees C H,Ettema T J G,et al.Identification and Molecular Characterization of a Novel Type of IⅠ±-galactosidase from Pyrococcus furiosus[J].Biocatalysis,2009,21(4~5):243~252.
[28] Luonteri E,Alatalo E,Siikaaho M,et al.alpha-Galactosidases of Penicillium simplicissimum:production,purification and characterization of the gene encoding AGLI[J].Biotechnology&Applied Biochemistry,1998,28(2):179~188.
[29] Muli mani V H,Ramalingam.Enzymic hydrolysis of raffinose and stachyose in soymilk by alpha-galactosidase from Gibberella fujikuroi[J].Biochemistry&Molecular Biology International,1995,36(4):897~905.
[30] Naumov D G.Phylogenetic analysis of a-galactosidases of the GH27 family[J].Molekuliarnaia Biologiia,2004,38(3):388~400.
[31] Okuyama M,Kitamura M,Hondoh H,et al.Catalytic mechanism of retaining alpha-galactosidase belonging to glycoside hydrolase family97[J].Journal of Molecular Biology,2009,392(5):1232~1241.
[32] Post D A,Luebke V E.Purification,cloning,and properties of al pha-galactosidase from Saccharopolyspora erythraea and its use as a reporter system[J].Applied Microbiology&Biotechnology,2005,67(1):91.
[33] Puchart A V,Vrsanska A M,Bhat B M K,et al.Purification and characterization ofα-galactosidase from a thermophilic fungus Thermomyces lanuginosus[J].Biochim Biophys Acta,2000,1524(1):27~37.
[34] Rezessyszab J M,Nguyen Q D,Hoschke A,et al.A novel thermostable alpha-galactosidase from the thermophilic fungus Thermomyces lanuginosus CBS 395.62/b:purification and characterization[J].Biochimica Et Biophysica Acta General Subjects,2007,1770(1):55~62.
[35] Shankar S K,Mulimani V H.Alpha-galactosidase production by Aspergillus oryzae in solid-state fermentation[J].Bioresource Technology,2007,98(4):958~961.
[36] Suresh C,Rus'd A A,Kitaokaa~M,et al.Evidence that the putative K-glucosidase of Thermotoga maritima MSB8 is a pNP KD-glucuronopyranoside hydrolyzing K-glucuronidase[J].FEBS Letters,2002,517(1~3):159~162.
[37] Suzuki H,Li S C,Li Y T,et al.Alpha-galactosidase from Mortierella vinacea.Crystallization and properties[J].Journal of Biological Chemistry,1970,245(4):781~786.
[38] Svastits-Dücs.o.Lilla,Nguyen Q D,Lefler D D,et al.Effects of galactomannan as carbon source on production ofα-galactosidase by Thermomyces lanuginosus:Fermentation,purification and partial characterization[J].Enzyme&Microbial Technology,2009,45(5):367~371.
[39] Thompson J,Pikis A,Ruvinov S B,et al.The gene glvA of Bacillus subtilis 168 encodes a metal-requiring,NAD(H)-dependent 6-phospho-alpha-glucosidase.Assignment to family 4 of the glycosylhydrolase superfamily[J].Journal of Biological Chemistry,1998,273(42):27347~27356.
[40] Ulezlo I V,Zaprometova O M.Microbial alpha-galactosidase(a review)[J].Prikladnaia Biokhimiia I Mikrobiologiia,1982,18(1):3.
[41] Vries R P D,Broeck H C V D,Dekkers E,et al.Differential Expression of Threeα-Galactosidase Genes and a Singleβ-Galactosidase Gene from Aspergillus niger[J].Applied&Environmental Microbiology,1999,65(6):2453.
[42] Wang H,Shi P,Luo H,et al.A thermophilicα-galactosidase from Neosartorya fischeri P1 with high specific activity,broad substrate specificity and significant hydrolysis ability of soymilk[J].Bioresource Technology,2014,153:361~364.
[43] Xiao M,Qian X Y K,Kumagai H,et al.High-yield production and characterization of alpha-galactosidase from Bifidobacterium breve grown on raffinose[J].Biotechnology Letters,2000,22(9):747~751.
[44] Zapater I G.Extracellular alpha galactosidase(E.C.3.2.1.22)from Aspergillus ficuum NRRL 3135 purification and characterization[J].Preparative Biochemistry,1990,20(3~4):263.
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