中药材菟丝子中金丝桃苷含量测定的影响因素研究
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摘要
目的:研究粉碎对菟丝子中金丝桃苷含量测定结果的影响。方法:采用不同效能的粉碎机、不同的粉碎时间等,对菟丝子药材进行粉碎,通过测定过筛后粉末中金丝桃苷的含量,以重点考察粉碎对测定结果的影响。结果:粉碎是影响菟丝子金丝桃苷含量高低的关键因素,而金丝桃苷在菟丝子种子不同部位的分布存在较大差异,其中种皮与最难粉碎部位中含量较低。结论:由于菟丝子较难粉碎,而金丝桃苷含量在种子各部位的分布不均,含量测定中的粉碎是关键操作步骤。本研究为进一步完善粉碎要求提供了一定的实验依据。
Objective: To investigate the influence of pulverization on the content determination of hyperoside in Semen Cuscutae. Methods: Semen Cuscutae was pulverized for the different time with the different pulverizers, and the content of hyperoside in the sieved powder was determined to evaluate the influence of pulverization on the test results. Results: Pulverization was the key influence factor of the content determination of hyperoside in Semen Cuscutae. The content of hyperoside in Semen Cuscutae was significantly different in different parts of the seeds, and the content of hyperoside in seed coat and hardest crushing part was lower. Conclusion: The pulverization was the most important operation step in the content determination of hyperoside because Semen Cuscutae was difficult to be pulverized and hyperoside was unevenly distributed in different parts of seeds. The study provided some experimental basis for the requirements on improvement of the pulverization.
Objective: To investigate the influence of pulverization on the content determination of hyperoside in Semen Cuscutae. Methods: Semen Cuscutae was pulverized for the different time with the different pulverizers, and the content of hyperoside in the sieved powder was determined to evaluate the influence of pulverization on the test results. Results: Pulverization was the key influence factor of the content determination of hyperoside in Semen Cuscutae. The content of hyperoside in Semen Cuscutae was significantly different in different parts of the seeds, and the content of hyperoside in seed coat and hardest crushing part was lower. Conclusion: The pulverization was the most important operation step in the content determination of hyperoside because Semen Cuscutae was difficult to be pulverized and hyperoside was unevenly distributed in different parts of seeds. The study provided some experimental basis for the requirements on improvement of the pulverization.
引文
[1] 中国药典2015年版.一部[S]. 2015:309ChP 2015. VolⅠ[S]. 2015:309
[2] 林慧彬,林建强,林建群,等.山东四种菟丝子补肾壮阳作用的比较[J]. 中成药, 2002, 24(5):354LIN HB, LIN JQ, LIN JQ, et al. Comparative study of effects on invigorating the kidney and strengthening Yang of four kinds of Semen Custutae in Shandong[J]. Chin Tradit Pat Med, 2002, 24(5):354
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[6] 李秋红, 王晓蕾, 李廷利,等. HPLC测定菟丝子炮制前后金丝桃苷和槲皮素的含量[J]. 中成药,2009,31(9):1394LI QH, WANG XL, LI TL, et al. Simultaneous determination of hyperoside and quercetin in crude and processed Semen Cuscutae by HPLC[J]. Chin Tradit Pat Med, 2009,31(9):1394
[7] 田雨霏, 刘姣,李晋,等. 高效液相色谱法测定菟丝子中绿原酸、对-香豆酸和金丝桃苷的含量[J]. 天津中医药大学学报,2014,33(6):372TIAN YF, LIU J, LI J, et al. Determination of chlorogenic acid, p-coumarin and hyperin in Semen Cuscutae by HPLC[J]. J Tianjin Univ Tradit Chin Med,2014,33(6):372
[8] 李怀国,叶家宏,李子鸿,等. HPLC 法同时测定菟丝子中5种成分的含量[J]. 中药新药与临床药理,2014,25(1):64LI HG, YE JH, LI ZH, et al. Content determination of five constitutes of Semen Cuscutae by HPLC[J]. Tradit Chin Drug Res Clin Pharm,2014,25(1):64
[2] 林慧彬,林建强,林建群,等.山东四种菟丝子补肾壮阳作用的比较[J]. 中成药, 2002, 24(5):354LIN HB, LIN JQ, LIN JQ, et al. Comparative study of effects on invigorating the kidney and strengthening Yang of four kinds of Semen Custutae in Shandong[J]. Chin Tradit Pat Med, 2002, 24(5):354
[3] 管群,吕圭源,俞静静,等. 不同产地菟丝子总黄酮及金丝桃苷的含量测定[J]. 浙江中医药大学学报,2012,36 (2):184GUAN Q, Lü GY, YU JJ, et al. Quantitative determination of total flavones and hyperoside in Semen Cuscutae collected from different places[J]. J Zhejiang Univ Tradit Chin Med,2012,36 (2):184
[4] 聂新华. 高效液相色谱法测定菟丝子中金丝桃苷的含量[J]. 中国实验方剂学杂志,2006,12(9):13NIE XH. Content determination of hyperoside in Semen Cuscutae by HPLC[J]. Chin J Exp Traditl Med Form,2006,12(9):13
[5] 侯秋苑,冯白茹,申茹. 菟丝子中金丝桃苷的提取工艺优选[J]. 汕头大学医学院学报,2017,30(3):141HOU QY, FENG BR, SHEN R. Optimization on the extraction process of hyperoside in Semen Cuscutae[J]. J Shantou Univ Med Coll,2017,30(3):141
[6] 李秋红, 王晓蕾, 李廷利,等. HPLC测定菟丝子炮制前后金丝桃苷和槲皮素的含量[J]. 中成药,2009,31(9):1394LI QH, WANG XL, LI TL, et al. Simultaneous determination of hyperoside and quercetin in crude and processed Semen Cuscutae by HPLC[J]. Chin Tradit Pat Med, 2009,31(9):1394
[7] 田雨霏, 刘姣,李晋,等. 高效液相色谱法测定菟丝子中绿原酸、对-香豆酸和金丝桃苷的含量[J]. 天津中医药大学学报,2014,33(6):372TIAN YF, LIU J, LI J, et al. Determination of chlorogenic acid, p-coumarin and hyperin in Semen Cuscutae by HPLC[J]. J Tianjin Univ Tradit Chin Med,2014,33(6):372
[8] 李怀国,叶家宏,李子鸿,等. HPLC 法同时测定菟丝子中5种成分的含量[J]. 中药新药与临床药理,2014,25(1):64LI HG, YE JH, LI ZH, et al. Content determination of five constitutes of Semen Cuscutae by HPLC[J]. Tradit Chin Drug Res Clin Pharm,2014,25(1):64