HPLC法测定山楂红曲胶囊中洛伐他汀
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摘要
目的建立山楂红曲胶囊中洛伐他汀的处理及含量测定方法。方法色谱柱为Kromasil C18柱(250 mm×4.6 mm,5μm),紫外检测波长238 nm,柱温25℃,体积流量1.0 mL/min,流动相为甲醇:0.1%磷酸溶液(75:25)。结果洛伐他汀质量浓度在2.04～306μg/mL与峰面积线性关系良好,平均回收率为98.58%,RSD=1.12%(n=9)。结论该方法准确、简便,可用于山楂红曲胶囊的质量控制。
Objective To establish a method of processing and determining for the lovastatin in Monascus Capsules. Methods Inspecting the influence of water bath temperature to the transformation of lovastatin structure, on the basis of "content determination standards for lovastatin in the health food". Chromatographic separation was achieved on a Kromasil C18 analytical column(250 mm × 4.6 mm, 5 μm), and the detection wavelength was set at 238 nm, maintained at 25 ℃. The prepared mobile phase was delivered at a flow rate of 1.0 mL/min. The mobile phase consisted of 75% Methano and 25% Phosphoric acid(0.1%).Results the linear range was 2.04 to 306 μg/mL. The average recovery was 98.58%. The precision was 1.12%(n = 9). Conclusion The improved method for determination is that transforming acid structure of lovastatin into lactone structure, the method which is accurate and simple can be applied to control the quality of Monascus Capsules.
Objective To establish a method of processing and determining for the lovastatin in Monascus Capsules. Methods Inspecting the influence of water bath temperature to the transformation of lovastatin structure, on the basis of "content determination standards for lovastatin in the health food". Chromatographic separation was achieved on a Kromasil C18 analytical column(250 mm × 4.6 mm, 5 μm), and the detection wavelength was set at 238 nm, maintained at 25 ℃. The prepared mobile phase was delivered at a flow rate of 1.0 mL/min. The mobile phase consisted of 75% Methano and 25% Phosphoric acid(0.1%).Results the linear range was 2.04 to 306 μg/mL. The average recovery was 98.58%. The precision was 1.12%(n = 9). Conclusion The improved method for determination is that transforming acid structure of lovastatin into lactone structure, the method which is accurate and simple can be applied to control the quality of Monascus Capsules.
引文
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[2]王玲,吴军林,吴清平.红曲降血脂功能的研究及应用概况[J].食品工业科技, 2014, 35(8):387-389, 393.
[3]保健食品检验与评价技术规范[S]. 2003.
[4]姜冰洁,许赣荣,张薄博,等.降脂红曲产品质量标准的探讨[J].中草药, 2015, 46(3):453-456.
[5]王丽君,崔海波.洛伐他汀开环工艺条件的研究[J].河北化工, 2008, 31(1):23-24.
[6]廖一民,向世学.高效液相同时测定保健食品中两种结构形式洛伐他汀的含量[J].中国卫生检验杂志, 2006,16(3):286-287, 315.
[7]程杰,刘姣,陈品英,等.高效液相色谱法测定脂必妥胶囊中洛伐他汀含量[J].中国药业, 2011, 20(8):36-37.
[8]陈东洋,冯家力,张昊,等.不同前处理方法对保健食品洛伐他汀含量测定的影响[J].食品工业科技, 2014, 11(35):295-297.
[9]陈发贵,张丹,刘敏,等.高效液相色谱法测定青稞红曲中内酯式和酸式洛伐他汀的含量[J].中国酿造,2016, 35(10):162-165.