姜厚朴饮片标准汤剂研究
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摘要
目的制备16批姜厚朴饮片标准汤剂,测定其出膏率、指标成分含量、转移率及指纹图谱等数据,为其配方颗粒的制备和质量评价提供参考。方法依照中药饮片标准汤剂制备要求,制备16批姜厚朴饮片标准汤剂,参照《中国药典》2015年版方法测定厚朴酚与和厚朴酚含量,计算两者总和的转移率,测定汤剂的出膏率及pH值,并绘制HPLC指纹图谱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱:0～15 min,12%～16%乙腈;15～30 min,16%～28%乙腈;30～42 min,28%～74%乙腈;42～55 min,74%～80%乙腈;柱温40℃;体积流量1 mL/min;检测波长294 nm。结果 16批姜厚朴饮片标准汤剂中厚朴酚与和厚朴酚总和的转移率为6.5%～12.0%,出膏率3.41%～7.14%,pH值4.63～5.43,并用中药色谱指纹图谱相似度评价系统(2012A)软件进行指纹图谱分析,找到了7个共有峰,指认4个,分别为木兰苷B(1号峰)、木兰苷A(2号峰)、和厚朴酚(6号峰)、厚朴酚(7号峰)。对16批姜厚朴饮片标准汤剂进行相似度评价,其相似度均大于0.69。同时,建立了姜厚朴饮片标准汤剂指纹图谱。结论所建立的汤剂制备方法稳定可行,分析方法精密度、稳定性和重复性良好,可用于姜厚朴饮片标准汤剂的质量评价。
Objective To study the dry extract rate, determination and transfer rate of maker compounds, and fingerprint of standard decoction of ginger juice Magnoliae Officinalis Cortex(GJMOC) and provide a reference for the preparation and quality assessment of its dispensing granules by establishing 16 batches of standard decoction of GJMOC. Methods A total of 16 batches of GJMOC standard decoctions were prepared following literature requirements. The quantitative analysis method of magnolol and honokiol was according to Chinese Pharmacopoeia(2015 edition). The transfer rate of total magnolol and honokiol and extraction rate were calculated. the pH value was determined and HPLC fingerprint was established under a flow rate of 1 mL/min and eluted with a mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) in a gradient mode(0—15 min, 12%—16% A; 15—30 min, 16%—28% A; 30—42 min, 28%—74% A; 42—55 min, 74%—80% A). The column temperature was set at 40℃ and the detection wavelength was 294 nm. Results By measuring the of 16 batches of standard decoction, the transfer rate of the sum of magnolol and honokiol ranged from 6.5% to 12.0%, the extraction rate was at a range of 3.41% to 7.14% and pH value was 4.63 to 5.43. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 A) was used to analyze and compare the fingerprints, and seven common peaks were determined and four were identified including magnoloside B(peak 1), magnoloside A(peak 2), honokiol(peak 6), and magnolol(peak 7). The similarity among 16 batches of standard decoction of GJMOC was evaluated, and the similarity was all greater than 0.69. Moreover, this study established an HPLC fingerprint analysis method of GJMOC standard decoction. Conclusion The preparation method established in this study is stable and feasible, and the analysis method shows good precision, stability, and repeatability in fingerprint analysis and it is suitable for evaluating the quality of standard decoction of GJMOC.
Objective To study the dry extract rate, determination and transfer rate of maker compounds, and fingerprint of standard decoction of ginger juice Magnoliae Officinalis Cortex(GJMOC) and provide a reference for the preparation and quality assessment of its dispensing granules by establishing 16 batches of standard decoction of GJMOC. Methods A total of 16 batches of GJMOC standard decoctions were prepared following literature requirements. The quantitative analysis method of magnolol and honokiol was according to Chinese Pharmacopoeia(2015 edition). The transfer rate of total magnolol and honokiol and extraction rate were calculated. the pH value was determined and HPLC fingerprint was established under a flow rate of 1 mL/min and eluted with a mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) in a gradient mode(0—15 min, 12%—16% A; 15—30 min, 16%—28% A; 30—42 min, 28%—74% A; 42—55 min, 74%—80% A). The column temperature was set at 40℃ and the detection wavelength was 294 nm. Results By measuring the of 16 batches of standard decoction, the transfer rate of the sum of magnolol and honokiol ranged from 6.5% to 12.0%, the extraction rate was at a range of 3.41% to 7.14% and pH value was 4.63 to 5.43. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 A) was used to analyze and compare the fingerprints, and seven common peaks were determined and four were identified including magnoloside B(peak 1), magnoloside A(peak 2), honokiol(peak 6), and magnolol(peak 7). The similarity among 16 batches of standard decoction of GJMOC was evaluated, and the similarity was all greater than 0.69. Moreover, this study established an HPLC fingerprint analysis method of GJMOC standard decoction. Conclusion The preparation method established in this study is stable and feasible, and the analysis method shows good precision, stability, and repeatability in fingerprint analysis and it is suitable for evaluating the quality of standard decoction of GJMOC.
引文
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[14]邓哲,焦梦姣,章军,等.含挥发油饮片-肉桂饮片标准汤剂质量标准研究[J].中国中药杂志,2018,43(5):885-890.
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[16]焦梦姣,邓哲,章军,等.含挥发性中药饮片标准汤剂的制备和质量标准研究-以牡丹皮为例[J].中国中药杂志,2018,43(5):891-896.
[17]钟凌云,兰智慧,祝婧,等.姜制前后厚朴毒性及刺激性研究[J].中成药,2013,35(8):1782-1785.
[18]陈淑珍.和厚朴酚的抗肿瘤实验治疗及其分子作用靶点的研究进展[J].药学学报,2016,51(2):202-207.
[19]刘畅,卲淑丽,夏艳,等.和厚朴酚对人肺癌A2细胞增殖和凋亡的影响[J].基因组学和应用生物学,2017,36(5):1797-1803.
[20]陈雄,虞伟慧,龚小花,等.厚朴酚通过MAPK/NF-κB信号通路改善I型糖尿病模型小鼠的心肌损伤[J].中草药,2017,48(22):4719-4725.
[21]盛安琪,刘文涛,张艳,等.和厚朴酚神经保护作用的研究进展[J].药学研究,2017,36(11):660-662.
[22]余盛贤.基于水溶性成分分析的厚朴质量评价[D].北京:中国中医科学院,2011.
[2]袁海建,李国银,姜俊,等.半夏-厚朴药对抗肿瘤功效物质基础及作用机制研究新思路[J].中草药,2018,49(8):1924-1931.
[3]许腊英,翁德会,李霞,等.炮制对厚朴饮片中厚朴酚、和厚朴酚含量变化规律的影响[J].中药材,2007,30(6):641-642.
[4]郭健,晏仁义,杨滨,等.炮制对厚朴主要化学成分影响[J].中国实验方剂学杂志,2012,18(15):117-120.
[5]李婷,杨舒然,陈敏,等.姜厚朴水提物对大肠杆菌和金黄色葡萄球菌的抑菌机理研究[J].现代食品科技,2016,32(2):84-92.
[6]许腊英,胡静,翁德会,等.净厚朴和姜厚朴水煎液主要药理学比较[J].中国医院药学,2007,27(11):1503-1505.
[7]黄大智,王建,田微,等.厚朴水煎液及其总酚提取物对胃肠动力和胃电慢波的影响[J].时珍国医国药,2015,26(3):528-530.
[8]中国药典[S].一部.2015.
[9]陈士林,刘安,李琦,等.中药饮片标准汤剂研究策略[J].中国中药杂志,2016,41(8):1367-1375.
[10]郭健,李化,杨滨,等.厚朴的炮制研究[J].中国实验方剂学,2011,17(2):258-262.
[11]钟凌云,张淑洁,龚千峰,等.生姜、干姜炮制对厚朴挥发性成分影响比较[J].中国实验方剂学杂志,2015,21(20):49-54.
[12]黄健,张辉,张建芳,等.不同炮制方法对厚朴中厚朴酚含量的影响[J].浙江中医药,2015,50(8):622-623.
[13]邓哲,冯伟红,章军,等.含挥发油饮片-桂枝饮片标准汤剂质量标准的建立及探讨[J].中国中药杂志,2017,42(14):2691.
[14]邓哲,焦梦姣,章军,等.含挥发油饮片-肉桂饮片标准汤剂质量标准研究[J].中国中药杂志,2018,43(5):885-890.
[15]邓哲,张凡,焦梦姣,等.姜黄饮片标准汤剂研究[J].中国中药杂志,2018,43(5):879-884.
[16]焦梦姣,邓哲,章军,等.含挥发性中药饮片标准汤剂的制备和质量标准研究-以牡丹皮为例[J].中国中药杂志,2018,43(5):891-896.
[17]钟凌云,兰智慧,祝婧,等.姜制前后厚朴毒性及刺激性研究[J].中成药,2013,35(8):1782-1785.
[18]陈淑珍.和厚朴酚的抗肿瘤实验治疗及其分子作用靶点的研究进展[J].药学学报,2016,51(2):202-207.
[19]刘畅,卲淑丽,夏艳,等.和厚朴酚对人肺癌A2细胞增殖和凋亡的影响[J].基因组学和应用生物学,2017,36(5):1797-1803.
[20]陈雄,虞伟慧,龚小花,等.厚朴酚通过MAPK/NF-κB信号通路改善I型糖尿病模型小鼠的心肌损伤[J].中草药,2017,48(22):4719-4725.
[21]盛安琪,刘文涛,张艳,等.和厚朴酚神经保护作用的研究进展[J].药学研究,2017,36(11):660-662.
[22]余盛贤.基于水溶性成分分析的厚朴质量评价[D].北京:中国中医科学院,2011.