5-氟尿嘧啶/明胶的制备及释药性能
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摘要
以A型明胶为原料、5-氟尿嘧啶为模型药物、异丙醇为凝聚剂,采用单凝聚法制备了5-氟尿嘧啶/明胶纳米载药颗粒(5-FU/GNPs),并测定了纳米颗粒的粒径分布、载药量、体外缓释效果和抗肿瘤性能。结果表明:5-FU/GNPs表面形态良好,分散均一,平均粒径为(75.1±2.1)nm,载药量为23.5%±1.9%;5-FU/GNPs具有良好的缓释性能,Higuchi方程对微球的体外药物释放情况拟合度较高。四甲基偶氮唑蓝实验表明:5-FU/GNPs对胃癌细胞(SGC7901)的抑制率可达71%。
5-Fluorouracil/gelatin nanoparticles(5-FU/GNPs) were prepared by simple coacervation method using type A gelatin as raw material,5-fluorouracil as entrapped pharmaceutical,isopropanol as coacervation reagent. The particle size distribution,drug loading,in vitro drug release and antitumor activities of 5-FU/GNPs were investigated. The results showed that 5-FU/GNPs had excellent surface morphology,and dispersed uniformly with an average particle size of(75.1±2.1) nm. The average drug loading was about 23.5%±1.9%. In vitro release experiments displayed that 5-FU/GNPs exhibited good release performance,the release of 5-fluorouracil from 5-FU/GNPs accorded with the Higuchi equation,indicating that the gelatin could sustain the release of 5-fluorouracil. MTT measurement showed that 5-FU/GNPs had significant cytotoxicity on human gastric cancer cells(SGC7901). Thus,the prepared 5-FU/GNPs could be used as a kind of drug-loading carrier in pharmaceutical field.
5-Fluorouracil/gelatin nanoparticles(5-FU/GNPs) were prepared by simple coacervation method using type A gelatin as raw material,5-fluorouracil as entrapped pharmaceutical,isopropanol as coacervation reagent. The particle size distribution,drug loading,in vitro drug release and antitumor activities of 5-FU/GNPs were investigated. The results showed that 5-FU/GNPs had excellent surface morphology,and dispersed uniformly with an average particle size of(75.1±2.1) nm. The average drug loading was about 23.5%±1.9%. In vitro release experiments displayed that 5-FU/GNPs exhibited good release performance,the release of 5-fluorouracil from 5-FU/GNPs accorded with the Higuchi equation,indicating that the gelatin could sustain the release of 5-fluorouracil. MTT measurement showed that 5-FU/GNPs had significant cytotoxicity on human gastric cancer cells(SGC7901). Thus,the prepared 5-FU/GNPs could be used as a kind of drug-loading carrier in pharmaceutical field.
引文
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