白藜芦醇抑制ApoE~(-/-)小鼠动脉血管氧化应激及炎症损伤的作用研究
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摘要
目的研究白藜芦醇抑制ApoE-/-小鼠动脉血管氧化应激及炎症损伤的作用。方法选取80只ApoE-/-小鼠给予高脂饲料饮食,制备动脉粥样硬化(AS)模型,并选取10只C3H小鼠做对照,给予白藜芦醇进行治疗,观察血脂及AS病变情况。结果白藜芦醇各剂量组小鼠各时间点的总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、动脉硬化指数(AI)水平、主动脉脂质蓄积面积、凋亡细胞指数均显著低于模型组(P<0.05)。白藜芦醇各剂量组丙二醛(MDA)、一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1及IL-6水平均显著低于模型组,超氧化物歧化酶(SOD)和谷胱甘肽(GSH)水平显著高于模型组(P<0.05)。白藜芦醇各剂量组p-JAK1及p-STAT3蛋白均显著低于模型组(P<0.05)。结论白藜芦醇可能通过抑制JAK1/STAT3通路减轻ApoE-/-小鼠动脉血管内的氧化应激及炎症损伤。
Objective To investigate the effects of resveratrol on oxidative stress and inflammatory injury of arterial blood vessels in ApoE-/-mice.Methods Eighty ApoE-/-mice were selected and given the high fat fodder for preparing the atherosclerotic model.Ten C3 H mice were selected as the control and given resveratrol for conducting the treatment.Then the changes of blood flat and atherosclerosis lesion were observed.The levels of malondialdehyde(MDA),nitric oxide(NO),superoxide dismutase(SOD)And glutathione(GSH),the expression of TNF-α,IL-1 and IL-6 and the expressions of IL-6,JAK1 and STAT3 proteins.Results The levels of TC,TG,LDL-C and AI,aortic lipid accumulation area and apoptotic cell index at each time point in the resveratrol doses groups were all significantly lower than those in the model group(P<0.05).The levels of MDA,NO,TNF-α,IL-1 and IL-6 in the resveratrol doses groups were significantly lower than those in model group,while the levels of SOD and GSH were significantly higher than those in the model group(P<0.05).The levels of p-JAK1 and p-STAT3 proteins in the resveratrol doses groups were significantly lower than those in the model group(P<0.05).Conclusion Resveratrol can alleviate the oxidative stress and inflammatory injury of arterial blood vessels in ApoE-/-mice by inhibiting the JAK1/STAT3 pathway.
Objective To investigate the effects of resveratrol on oxidative stress and inflammatory injury of arterial blood vessels in ApoE-/-mice.Methods Eighty ApoE-/-mice were selected and given the high fat fodder for preparing the atherosclerotic model.Ten C3 H mice were selected as the control and given resveratrol for conducting the treatment.Then the changes of blood flat and atherosclerosis lesion were observed.The levels of malondialdehyde(MDA),nitric oxide(NO),superoxide dismutase(SOD)And glutathione(GSH),the expression of TNF-α,IL-1 and IL-6 and the expressions of IL-6,JAK1 and STAT3 proteins.Results The levels of TC,TG,LDL-C and AI,aortic lipid accumulation area and apoptotic cell index at each time point in the resveratrol doses groups were all significantly lower than those in the model group(P<0.05).The levels of MDA,NO,TNF-α,IL-1 and IL-6 in the resveratrol doses groups were significantly lower than those in model group,while the levels of SOD and GSH were significantly higher than those in the model group(P<0.05).The levels of p-JAK1 and p-STAT3 proteins in the resveratrol doses groups were significantly lower than those in the model group(P<0.05).Conclusion Resveratrol can alleviate the oxidative stress and inflammatory injury of arterial blood vessels in ApoE-/-mice by inhibiting the JAK1/STAT3 pathway.
引文
[1]卢克红,潘旭东,马爱军,等.半乳糖凝集素-3与脑动脉粥样硬化广泛程度及大动脉粥样硬化型脑卒中预后的关系[J].中华神经科杂志,2016,49(7):531-535.
[2]常琦,金萍,张晓玲,等.动脉粥样硬化早期干预与预防冠心病中的作用[J].中国老年学杂志,2014,12(9):2367-2369.
[3]李佳军,韩金良,田玉顺.白藜芦醇及其衍生物药理作用的研究现状[J].药学研究,2017,36(3):161-163.
[4]胡洵,庄晓东,周莹.白藜芦醇对过氧化氢诱导人动脉平滑肌细胞的影响及其机制[J].广州医学院学报,2013,41(4):1-4.
[5]秦丽,关莉,林锐珊,等.白藜芦醇通过调节iNOS抑制C57BL/6J小鼠动脉粥样硬化的机制探讨[J].中国病理生理杂志,2015(9):1601-1605.
[6]张彦,马双陶,杨永健.糖尿病合并动脉粥样硬化小鼠模型的建立[J].中国动脉硬化杂志,2014,22(2):186-189.
[7]刘季晨.血清淀粉样P物质对巨噬细胞炎症反应以及氧化应激的影响及机制[D].广州:南方医科大学,2016.
[8]刘应柯,张玉峰,刘尚岭,等.脂脉宁对动脉粥样硬化家兔氧化应激反应及LDL-R,VCAM-1基因表达的影响[J].中国实验方剂学杂志,2011,17(20):214-217.
[9]徐能全,邱启祥,周小龙,等.下丘脑-垂体轴反馈调节方式对内皮细胞MDA代谢和NOS表达的影响[J].重庆医学,2011,40(8):742-743,751.
[10]赵彦,赵美丽,林爱翠.等长收缩运动对动脉粥样硬化兔血管内皮生长因子与一氧化氮的影响[J].中华物理医学与康复杂志,2016,38(10):726-729.
[11]赵洪宇,蒋梦会,曹久妹,等.IL-6/STAT3通路对THP-1单核细胞环氧化酶2表达的影响[J].中国动脉硬化杂志,2016,24(3):251-255,260.
[12]姜红菊,李润智,杜修文,等.宁心解毒汤、血塞通、瑞舒伐他汀对ApoE-/-小鼠炎症因子和As斑块的影响[J].中国动脉硬化杂志,2016,24(12):1214-1218.
[2]常琦,金萍,张晓玲,等.动脉粥样硬化早期干预与预防冠心病中的作用[J].中国老年学杂志,2014,12(9):2367-2369.
[3]李佳军,韩金良,田玉顺.白藜芦醇及其衍生物药理作用的研究现状[J].药学研究,2017,36(3):161-163.
[4]胡洵,庄晓东,周莹.白藜芦醇对过氧化氢诱导人动脉平滑肌细胞的影响及其机制[J].广州医学院学报,2013,41(4):1-4.
[5]秦丽,关莉,林锐珊,等.白藜芦醇通过调节iNOS抑制C57BL/6J小鼠动脉粥样硬化的机制探讨[J].中国病理生理杂志,2015(9):1601-1605.
[6]张彦,马双陶,杨永健.糖尿病合并动脉粥样硬化小鼠模型的建立[J].中国动脉硬化杂志,2014,22(2):186-189.
[7]刘季晨.血清淀粉样P物质对巨噬细胞炎症反应以及氧化应激的影响及机制[D].广州:南方医科大学,2016.
[8]刘应柯,张玉峰,刘尚岭,等.脂脉宁对动脉粥样硬化家兔氧化应激反应及LDL-R,VCAM-1基因表达的影响[J].中国实验方剂学杂志,2011,17(20):214-217.
[9]徐能全,邱启祥,周小龙,等.下丘脑-垂体轴反馈调节方式对内皮细胞MDA代谢和NOS表达的影响[J].重庆医学,2011,40(8):742-743,751.
[10]赵彦,赵美丽,林爱翠.等长收缩运动对动脉粥样硬化兔血管内皮生长因子与一氧化氮的影响[J].中华物理医学与康复杂志,2016,38(10):726-729.
[11]赵洪宇,蒋梦会,曹久妹,等.IL-6/STAT3通路对THP-1单核细胞环氧化酶2表达的影响[J].中国动脉硬化杂志,2016,24(3):251-255,260.
[12]姜红菊,李润智,杜修文,等.宁心解毒汤、血塞通、瑞舒伐他汀对ApoE-/-小鼠炎症因子和As斑块的影响[J].中国动脉硬化杂志,2016,24(12):1214-1218.