显齿蛇葡萄中黄酮类化合物对α-葡萄糖苷酶的抑制作用研究
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摘要
目的:研究显齿蛇葡萄醇提物及8种主要黄酮化合物对α-葡萄糖苷酶的抑制作用。方法:用75%无水乙醇回流提取制备显齿蛇葡萄提取物,对其进行α-葡萄糖苷酶抑制实验。之后选取显齿蛇葡萄中8种主要黄酮化合物对α-葡萄糖苷酶的抑制效果进行分析。结果:醇提物在(0.1~1.0)mg/mL之间对α-葡萄糖苷酶具有明显的抑制作用,其半抑制浓度(IC50)为0.338mg/mL。8种黄酮化合物均对α-葡萄糖苷酶产生不同程度的抑制作用,其中槲皮素的抑制效果最强,半抑制浓度为0.083 mg/mL,是醇提物的1/4。结论:显齿蛇葡萄醇提物与主要黄酮化合物对α-葡萄糖苷酶具有较明显的抑制作用,为显齿蛇葡萄进一步开发与利用提供了理论基础。
Objective: The inhibition of alcohol extract and eight kinds of main flavonoids from Ampelopsis grossedentata on α-glucosidase activity were studied. Methods: Extraction 75% anhydrous ethanol from Ampelopsis grossedentata extract and inhibition of α-glucosidase were investigated. After that, the eight main flavonoids from Ampelopsis grossedentata the were selected to analyze the inhibitory effects of α-glucosidase. Results: The absolute ethanol extract had a significant inhibitory effect on α-glucosidase between 0.1 and 1.0 mg/mL, and its semi-inhibitory concentration(IC50) was 0.338 mg/mL. All the eight flavonoids had different degrees of inhibition on α-glucosidase. Among them, quercetin had the strongest inhibitory effect, the IC50 was 0.083 mg/mL, which was 0.25 times higher that of the alcohol extract. Conclusion: The ethanol extract and flavonoids of Ampelopsis grossedentata had a significant inhibitory effect on α-glucosidase, which provided a theoretical basis for the further development and utilization of Ampelopsis grossedentata.
Objective: The inhibition of alcohol extract and eight kinds of main flavonoids from Ampelopsis grossedentata on α-glucosidase activity were studied. Methods: Extraction 75% anhydrous ethanol from Ampelopsis grossedentata extract and inhibition of α-glucosidase were investigated. After that, the eight main flavonoids from Ampelopsis grossedentata the were selected to analyze the inhibitory effects of α-glucosidase. Results: The absolute ethanol extract had a significant inhibitory effect on α-glucosidase between 0.1 and 1.0 mg/mL, and its semi-inhibitory concentration(IC50) was 0.338 mg/mL. All the eight flavonoids had different degrees of inhibition on α-glucosidase. Among them, quercetin had the strongest inhibitory effect, the IC50 was 0.083 mg/mL, which was 0.25 times higher that of the alcohol extract. Conclusion: The ethanol extract and flavonoids of Ampelopsis grossedentata had a significant inhibitory effect on α-glucosidase, which provided a theoretical basis for the further development and utilization of Ampelopsis grossedentata.
引文
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[2]张学娟.藤茶的形态学、生物学特性及主要化学成分研究[D].武汉:华中农业大学,2008
[3]汲军,王丹丹,赵昱伟.显齿蛇葡萄叶有效成分的提取工艺研究[J].食品安全导刊,2016,26:69
[4]付明,黎晓英,王登宇,等.显齿蛇葡萄叶中黄酮类化合物的研究[J].中国药学杂志,2015,(07)
[5]何桂霞,裴刚,周天达,等.显齿蛇葡萄中总黄酮和二氢杨梅素的含量测定[J].中国中药杂志,2000,(07):39-41
[6]杨丽丽,石治敏,焦思棋,等.显齿蛇葡萄种皮中花色苷及多糖体外抗氧化活性评价[J].食品工业科技,2017,38(16):65-68,87
[7]陈帅,郁建平.藤茶总黄酮抗炎及抑菌作用的实验研究[J].贵阳中医学院学报,2013,35(1):1-3
[8]漆姣媚,蒋燕群,张杰,等.显齿蛇葡萄总黄酮降血糖作用研究[J].中国药学志,2017,52(19):1685-1690
[9]张斌.显齿蛇葡萄提取物的体内和体外降血糖效果[J].中国老年学杂志,2017,37(02):321-323
[10] Lu A, Tang Y, Ran R, et al. Brain genomics of intracerebral hemorrhage[J]. J Cereb Blood Flow Metab,2006,26(2):230-252
[11]廖寅平,王硕,安丰轩,等.藤茶降血压作用研究[J].中国现代药物应用,2013,17(7):229-230
[12] Lei Liu, Jing Wan, Hedong Lang, et al. Dihydromyricetin delays the onset of hyperglycemia and ameliorates insulin resistance without excessive weight gain in Zucker diabetic fatty rats[J]. MoLecular and Cellular Endocrinology,2017,439:105-115
[13] Yao Y,Sang W,Zhou M,et al. Antioxidant and a-Glucosidase inhibitory activity of colored grains in China[J]. Journal of Agricultural and Food Chemistry,2010,58(2):770-774
[14]韩林,刘益,罗梅,等.杨梅素对α-葡萄糖苷酶的抑制活性机制研究[J].食品工业科技,2017,38(21):51-60
[15]王家胜.显齿蛇葡萄中黄酮化合物代谢及相关性研究[D].贵阳:贵州师范大学,2015
[16]曾云想,郁建平.藤茶总黄酮提取方法的优选[J].山地农业生物学报,2013,32(02):153-158
[17]王斯慧,白银花,黄琬凌,等.苦荞黄酮对α-葡萄糖苷酶的抑制作用研究[J].食品科技,2012,37(02):24-26,31
[18]王斯慧,黄琬凌,陈庆松,等.芦丁、槲皮素对α-葡萄糖苷酶活性抑制研究[J].中国酿造,2012,31(01):133-135
[19]屠洁,陈钧,刘冠卉.芹菜素抑制α-葡萄糖苷酶的分子机制研究[J].生物技术,2015,25(04):369-374
[20] Jia Chen, Yuechan Wu, Jianwei Zou, et al. a-Glucosidase inhibition and antihyperglycemic activity of flavonodis from Ampelopsis grossedentata and the flavonoid derivatives[J]. Bioorganic and Medicinal Chemistry,2016,24:1488-1494