Con A建立小鼠急性免疫性肝损伤模型研究
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摘要
目的探讨刀豆蛋白A(Con A)诱导急性免疫性肝损伤小鼠模型的最适剂量及最佳给药途径.方法选用雄性ICR小鼠180只,分为尾静脉注射与腹腔注射两种给药途径,每个给药途径分为6组,对照组、Con A 10,20,30,40和50 mg/kg剂量组,Con A各剂量组注射相应剂量的Con A,对照组注射生理盐水,在注射8 h后,检测各组血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)的水平,采用苏木素-伊红(HE)染色法观察肝组织病理变化.结果与对照组比较,腹腔注射Con A各剂量组的ALT和AST水平无明显变化;尾静脉注射Con A 40 mg/kg剂量组的ALT和AST水平明显升高(P<0. 05);组织学病理显示:肝损伤明显,Con A 10,20,30 mg/kg剂量组ALT和AST水平变化不明显,50 mg/kg剂量组死亡率较高.结论小鼠尾静脉注射40 mg/kg的Con A可成功诱导ICR小鼠的急性肝损伤.
Objective To investigate the optimal dose and route of Con A administration on acute immunological liver injury in mice. Method 180 ICR mice were divided into intraperitoneal and intravenous injection of two routes of administration. Each route was divided into control group,Con A 10,20,30,40,50 mg/kg dose groups.The mice in Con A group were injected with Con A and those in control group were intraperitoneally injected with normal saline. After the administration for 8 h,the levels of Aspartate aminotransferase(AST) and Alanine aminotransferase(ALT) were measured,the histomorphological liver were observed by hematoxylin and eosin(HE) stain. Results Compared with the control group,there were not significantly changed in the levels of AST and ALT by intraperitoneal injection. While in intravenous injection group,the levels of AST and ALT of mice in Con A 40 mg/kg group were increased obviously and the histomorphological examination of liver indicated that there was an obvious injury,and those in Con A 10,20,30 mg/kg groups were not significantly changed. The mortality rate was higher in 50 mg/kg dose group. Conclusion Intravenous injection of 40 mg/kg Con A can induce acute liver injury in ICR mice.
Objective To investigate the optimal dose and route of Con A administration on acute immunological liver injury in mice. Method 180 ICR mice were divided into intraperitoneal and intravenous injection of two routes of administration. Each route was divided into control group,Con A 10,20,30,40,50 mg/kg dose groups.The mice in Con A group were injected with Con A and those in control group were intraperitoneally injected with normal saline. After the administration for 8 h,the levels of Aspartate aminotransferase(AST) and Alanine aminotransferase(ALT) were measured,the histomorphological liver were observed by hematoxylin and eosin(HE) stain. Results Compared with the control group,there were not significantly changed in the levels of AST and ALT by intraperitoneal injection. While in intravenous injection group,the levels of AST and ALT of mice in Con A 40 mg/kg group were increased obviously and the histomorphological examination of liver indicated that there was an obvious injury,and those in Con A 10,20,30 mg/kg groups were not significantly changed. The mortality rate was higher in 50 mg/kg dose group. Conclusion Intravenous injection of 40 mg/kg Con A can induce acute liver injury in ICR mice.
引文
[1]李红,于慧杰,盛国光.刀豆蛋白A诱导的C57BL/6J小鼠急性免疫性肝损伤模型的研究[J].中西医结合肝病杂志,2016,26(2):93-95,131.
[2]于慧杰,李红,郭明星,等. Con A不同给药途径所致C57小鼠急、慢性肝损伤模型的建立与比较[J].湖北中医药大学学报,2014,16(4):5-7.
[3]周琼,刘芳萍,刘颖姝,等.四氯化碳致小鼠急性肝损伤动物模型建立方法的研究[J].东北农业大学学报,2012,43(6):77-81.
[4] Kaneko Y,Harada M,Kawano T,et al. Augmentation of Vα14 NKT cell mediated cytotoxicity by interleukin 4 in an autocrine mechanism resulting in the development of concanavalin A induced hepatits[J]. J Exp Med,2000,191(1):105-114.
[5] Tiegs G,Hentschel J,Wendel A. A T cell-dependent experimental liver injury in mice inducible by concanavalin A[J]. Journal of Clinical Investigation,1992,90(1):196-203.
[6]张雯,黄磊,陈叶香,等.复方龙垂草水煎剂对CCl4致小鼠急性肝损伤的保护作用[J].中药新药与临床药理,2015,26(3):280-284.
[7]刘慧敏,韩延忠,郭玉明,等.六味五灵片对刀豆蛋白A诱导的小鼠急性免疫性肝损伤的保护作用研究[J].中国药理学通报,2017,33(1):133-140.
[8]张炜,陈明,傅涓涓.不同剂量刀豆蛋白A诱导Balb/c小鼠肝纤维化的量效关系[J].徐州医学院学报,2007,27(6):375-378.
[9]马静.刀豆蛋白A致肝损伤模型条件优化及痰热清注射液对其保护作用研究[A].中国毒理学会中药与天然药物毒理专业委员会.中国毒理学会中药与天然药物毒理专业委员会第一次(2016年)学术交流大会论文集[C].中国毒理学会中药与天然药物毒理专业委员会,2016:2.
[10]刘悦晖,范学工,李宁,等.刀豆球蛋白A所致实验性肝损伤模型的构建[J].中国感染控制杂志,2008,7(5):297-301.
[2]于慧杰,李红,郭明星,等. Con A不同给药途径所致C57小鼠急、慢性肝损伤模型的建立与比较[J].湖北中医药大学学报,2014,16(4):5-7.
[3]周琼,刘芳萍,刘颖姝,等.四氯化碳致小鼠急性肝损伤动物模型建立方法的研究[J].东北农业大学学报,2012,43(6):77-81.
[4] Kaneko Y,Harada M,Kawano T,et al. Augmentation of Vα14 NKT cell mediated cytotoxicity by interleukin 4 in an autocrine mechanism resulting in the development of concanavalin A induced hepatits[J]. J Exp Med,2000,191(1):105-114.
[5] Tiegs G,Hentschel J,Wendel A. A T cell-dependent experimental liver injury in mice inducible by concanavalin A[J]. Journal of Clinical Investigation,1992,90(1):196-203.
[6]张雯,黄磊,陈叶香,等.复方龙垂草水煎剂对CCl4致小鼠急性肝损伤的保护作用[J].中药新药与临床药理,2015,26(3):280-284.
[7]刘慧敏,韩延忠,郭玉明,等.六味五灵片对刀豆蛋白A诱导的小鼠急性免疫性肝损伤的保护作用研究[J].中国药理学通报,2017,33(1):133-140.
[8]张炜,陈明,傅涓涓.不同剂量刀豆蛋白A诱导Balb/c小鼠肝纤维化的量效关系[J].徐州医学院学报,2007,27(6):375-378.
[9]马静.刀豆蛋白A致肝损伤模型条件优化及痰热清注射液对其保护作用研究[A].中国毒理学会中药与天然药物毒理专业委员会.中国毒理学会中药与天然药物毒理专业委员会第一次(2016年)学术交流大会论文集[C].中国毒理学会中药与天然药物毒理专业委员会,2016:2.
[10]刘悦晖,范学工,李宁,等.刀豆球蛋白A所致实验性肝损伤模型的构建[J].中国感染控制杂志,2008,7(5):297-301.
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