5种条件下桉树焦枯病菌MAPK基因的表达
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摘要
采用qPCR检测Calonectria pseudoreteaudii YA51的MAPK基因在盐胁迫、氧胁迫、细胞壁干扰物质胁迫、桉树组织诱导以及不同发育时期5种条件下的表达情况.结果表明CpKss1参与致病过程,CpKss1、CpSlt2和CpIme2与菌丝生长发育有关,CpKss1、CpHog1和CpIme2参与病菌高渗胁迫反应,CpSlt2参与调控氧胁迫反应和细胞壁完整性,且4个MAPK基因所介导的级联通路间可能存在既相互独立又相互协作的关系.
Expression of MAPK genes of Calonectria pseudoreteaudii YA51 under salt stress, H_2O_2 stress, CFW stress, infection, and at different developmental stages were quantatively analyzed by qPCR. According to the expression levels, CpKss1 is likely to be involved in the regulation of pathogenicity; CpKss1, CpSlt2 and CpIme2 are involved in the mycelial growth and development; CpKss1, CpHog1 and CpIme2 participate under hypertonic condition; CpSlt2 is involved in the maintaining of cell wall integrity and the regulation under H_2O_2 stress. Furthermore, there were mutually independent and cooperative relationships in the signaling cascades mediated by the 4 genes.
Expression of MAPK genes of Calonectria pseudoreteaudii YA51 under salt stress, H_2O_2 stress, CFW stress, infection, and at different developmental stages were quantatively analyzed by qPCR. According to the expression levels, CpKss1 is likely to be involved in the regulation of pathogenicity; CpKss1, CpSlt2 and CpIme2 are involved in the mycelial growth and development; CpKss1, CpHog1 and CpIme2 participate under hypertonic condition; CpSlt2 is involved in the maintaining of cell wall integrity and the regulation under H_2O_2 stress. Furthermore, there were mutually independent and cooperative relationships in the signaling cascades mediated by the 4 genes.
引文
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[23] KOJIMA K,KIKUCHI T,TAKANO Y,et al.The mitogen-activated protein kinase gene MAF1 is essential for the early differentiation phase of appressorium formation in Colletotrichum lagenarium [J].Mol Plant Microbe Interact,2002,15(12):1 268-1 276.
[24] HOU Z,XUE C,PENG Y,et al.A mitogen-activated protein kinase gene ( MGV 1) in Fusarium graminearum is required for female fertility,heterokaryon formation,and plant infection[J].Mol Plant Microbe Interact,2002,15(11):1 119-1 127.
[25] PARK S M,CHOI E S,KIM M J,et al.Characterization of HOG1 homologue,CpMK1,from Cryphonectria parasitica and evidence for hypovirus-mediated perturbation of its phosphorylation in response to hypertonic stress[J].Mol Microbiol,2004,51(5):1 267-1 277.
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[27] MORIWAKI A,KUBO E,ARASE S,et al.Disruption of SRM1,a mitogen-activated protein kinase gene,affects sensitivity to osmotic and ultraviolet stressors in the phytopathogenic fungus Bipolaris oryzae[J].FEMS Microbiol Lett,2006,257(2):253-261.
[28] IENIGER S.The Ime2 protein kinase family in fungi:more duties than just meiosis[J].Mol Microbiol,2011,80(1):1-13.
[2] LOMBARD L,CHEN S F,MOU X,et al.New species,hyper-diversity and potential importance of Calonectria spp.from Eucalyptus in South China[J].Studies in Mycology,2015,80:88-151.
[3] 朱建华,郭文硕,陈红梅,等.桉树焦枯病对桉树生长量的损失估计研究[J].中国森林病虫,2011,30(5):6-10.
[4] 陈全助,郭文硕,叶小真,等.福建省桉树焦枯病菌分类鉴定[J].福建林学院学报,2013,33(2):176-182.
[5] KOU Y,NAQIV N I.Surface sensing and signaling networks in plant pathogenic fungi[J].Seminars in Cell & Developmental Biology,2016,57:84-92.
[6] JIANG C,ZHANG X ,LIU H,et al.Mitogen-activated protein kinase signaling in plant pathogenic fungi[J].Plos Pathogens,2018,14(3):e1006875.
[7] HAMEL L P,NICOLE M C,DUPEEISS S,et al.Mitogen-activated protein kinase signaling in plant interacting fungi:distinct messages from conserved messengers[J].Plant Cell,2012,24(4):27-51.
[8] 彭静静.植物病原真菌中MAPK级联通路研究进展[J].江苏农业科学,2014(9):11-15.
[9] ONYILO F,TUSIIME G,TRIPHTI J N,et al.Silencing of the mitogen-activated protein kinases (MAPK) Fus3 and Slt2 in Pseudocercospora fijiensis reduces growth and virulence on host plants[J].Frontiers in Plant Science,2018,9:291.
[10] HE P,WANG Y,WANG X,et al.The mitogen-activated protein kinase CgMK1 governs appressorium formation,melanin synthesis,and plant infection of Colletotrichum gloeosporioides[J].Front Microbiol.2017,8:2 216.
[11] KOJIMA K,TAKANO Y,YOSHIMI A,et al.Fungicide activity through activation of a fungal signalling pathway[J].Mol Microbiol,2004,53(6):1 785-1 796.
[12] 李征,刘登义,王育鹏,等.活性氧在植物——病原物相互作用过程中的作用[J].安徽师范大学学报(自然科学版),2006(1):70-74.
[13] 张玉,杨爱国,冯全福,等.植物病程相关蛋白及其在烟草中的研究进展[J].生物技术通报,2012(5):20-24.
[14] 张永军,赵建华,蒋小东,等.球孢白僵菌Hog1 MAPK同源基因BbHog1的克隆及特征分析[J].菌物学报,2007(1):76-83.
[15] 陈慧洁,冯丽贞,李慧敏,等.桉树焦枯病菌内参基因的筛选[J].森林与环境学报,2018,38(1):98-103.
[16] SCHIMITTGEN T D,LIVAK K J.Analyzing real-time PCR data by the comparative C(T) method[J].Nat Protoc,2008,3(6):1 101-1 108.
[17] MULLER P,WEINZIERL G,BRACHMANN A,et al.Mating and pathogenic development of the Smut fungus Ustilago maydis are regulated by one mitogen-activated protein kinase cascade[J].Eukaryot Cell,2003,2(6):1 187-1 199.
[18] TAKANO Y,KIKUCHI T,KUBO Y,et al.The Colletotrichum lagenarium MAP kinase gene CMK1 regulates diverse aspects of fungal pathogenesis[J].Mol Plant Microbe Interact,2000,13(4):374-383.
[19] COUSIN A,MEHRABI R,GUILLEROUX M,et al.The MAP kinase-encoding gene MgFus3 of the non appressorium phytopathogen Mycosphaerella graminicola is required for penetration and in vitro pycnidia formation[J].Molecular Plant Pathology,2006,7(4):269-278.
[20] SOLOMON P S,WATER O D,SIMMONDS J,et al.The Mak2 MAP kinase signal transduction pathway is required for pathogenicity in Stagonospora nodorum[J].Curr Genet,2005,48(1):60-68.
[21] HAMEL L P,NICOLE M C,DUPLESSIS S,et al.Mitogen-activated protein kinase signaling in plant interacting fungi:distinct messages from conserved messengers[J].Plant Cell,2012,24(4):1 327-1 351.
[22] ZENG F,GONG X,HAMID M I,et al.A fungal cell wall integrity-associated MAP kinase cascade in Coniothyrium minitans is required for conidiation and mycoparasitism[J].Fungal Genet Biol,2012,49(5):347-357.
[23] KOJIMA K,KIKUCHI T,TAKANO Y,et al.The mitogen-activated protein kinase gene MAF1 is essential for the early differentiation phase of appressorium formation in Colletotrichum lagenarium [J].Mol Plant Microbe Interact,2002,15(12):1 268-1 276.
[24] HOU Z,XUE C,PENG Y,et al.A mitogen-activated protein kinase gene ( MGV 1) in Fusarium graminearum is required for female fertility,heterokaryon formation,and plant infection[J].Mol Plant Microbe Interact,2002,15(11):1 119-1 127.
[25] PARK S M,CHOI E S,KIM M J,et al.Characterization of HOG1 homologue,CpMK1,from Cryphonectria parasitica and evidence for hypovirus-mediated perturbation of its phosphorylation in response to hypertonic stress[J].Mol Microbiol,2004,51(5):1 267-1 277.
[26] MEHRABI R,ZWIERS L H,DEWAARD M A,et al.MgHog1 regulates dimorphism and pathogenicity in the fungal wheat pathogen Mycosphaerella graminicola [J].Mol Plant Microbe Interact,2006,19(11):1 262-1 269.
[27] MORIWAKI A,KUBO E,ARASE S,et al.Disruption of SRM1,a mitogen-activated protein kinase gene,affects sensitivity to osmotic and ultraviolet stressors in the phytopathogenic fungus Bipolaris oryzae[J].FEMS Microbiol Lett,2006,257(2):253-261.
[28] IENIGER S.The Ime2 protein kinase family in fungi:more duties than just meiosis[J].Mol Microbiol,2011,80(1):1-13.
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