厚朴及其提取物体外抗流感病毒H1N1的初步研究
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摘要
目的观察厚朴及其提取物体外抗流感病毒H1N1的作用。方法经鸡胚扩增流感病毒H1N1,并测定其感染MDCK细胞的TCID50;采用MTT法检测药物对细胞的毒性及三种给药方式下药物对病毒的抑制作用,并进一步通过流式细胞仪初步探讨药物抗病毒机制。结果H1N1的TCID50为1.3×10~(-9)·L~(-1);厚朴酚、厚朴水浸液及厚朴水煎液的最大无毒浓度分别为1.2μg·mL~(-1)、10.8mg·mL~(-1)及2400μg·mL~(-1);三种给药方式厚朴水煎液对病毒均无作用,厚朴酚有一定的治疗效果,厚朴水浸液有预防和治疗效果;流式细胞仪结果说明厚朴酚和厚朴水浸液的治疗作用可能是通过调整细胞周期、提高细胞增殖指数而实现的。结论对流感病毒H1N1而言,厚朴酚和厚朴水浸液对其有一定的治疗效果,厚朴水浸液对其有一定的预防作用,而厚朴水煎液对其没有作用。
OBJECTIVE To explore the inhibitory effects of Mangnolia Officinalis and its extractive against influenza virus H1 N1. METHODS Propagation of virus in chick embryo and the value of TCID50 were calculated; The MTT test assay was used to evaluate the toxicity and the inhibitory effect of drugs against H1 N1,and through Flow Cytometry to discuss the antiviral mechanism of Mangnolia Officinalis and its extractive. RESULTS The TCID50 of H1N1 virus to MDCK cells was 1. 3 × 10~(-9)·L~(-1). The TC0 of Magnolol,Mangnolia Officinalis immersion and decoction in MDCK cells were 1. 2μg·mL~(-1),10. 8 mg·mL~(-1) and 2400μg·mL~(-1). Three kinds of way of magnolia officinalis decoction on virus had no effect. Magnolol had a certain therapeutic effect. Mangnolia Officinalis immersion had prevention and therapeutic effect. Flow cytometry results demonstrated the therapeutic effect of Magnolol and Mangnolia Officinalis immersion may be improved through the adjustment of the cell cycle and cell proliferation index. CONCLUSION For H1 N1 influenza virus,Magnolol and Mangnolia Officinalis immersion had certain therapeutic effect,Mangnolia Officinalis immersion had certain preventive effect,and magnolia officinalis decoction has no effect.
OBJECTIVE To explore the inhibitory effects of Mangnolia Officinalis and its extractive against influenza virus H1 N1. METHODS Propagation of virus in chick embryo and the value of TCID50 were calculated; The MTT test assay was used to evaluate the toxicity and the inhibitory effect of drugs against H1 N1,and through Flow Cytometry to discuss the antiviral mechanism of Mangnolia Officinalis and its extractive. RESULTS The TCID50 of H1N1 virus to MDCK cells was 1. 3 × 10~(-9)·L~(-1). The TC0 of Magnolol,Mangnolia Officinalis immersion and decoction in MDCK cells were 1. 2μg·mL~(-1),10. 8 mg·mL~(-1) and 2400μg·mL~(-1). Three kinds of way of magnolia officinalis decoction on virus had no effect. Magnolol had a certain therapeutic effect. Mangnolia Officinalis immersion had prevention and therapeutic effect. Flow cytometry results demonstrated the therapeutic effect of Magnolol and Mangnolia Officinalis immersion may be improved through the adjustment of the cell cycle and cell proliferation index. CONCLUSION For H1 N1 influenza virus,Magnolol and Mangnolia Officinalis immersion had certain therapeutic effect,Mangnolia Officinalis immersion had certain preventive effect,and magnolia officinalis decoction has no effect.
引文
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[10]张耕实,祁贤,卢协勤,等.栀子苷对甲型H1N1流感病毒的抑制作用[J].中国药科大学学报,2016,47(2):204-209.
[2]陈笈,王伯初.厚朴的药理研究进展[J].重庆大学学报(自然科学版),2005,28(9):136-139.
[3]冯劲立,汪德龙,张奉学.新加香薷饮及其组方药物抗甲1型流感病毒作用的比较研究[J].湖南中医药大学学报,2010,3(1):31-33.
[4]戴自英.临床抗菌药物学[M].第1版.北京:人民卫生出版社,1985,377-378.
[5]王欢,张能英,孙兴泽,等.八角茴香及其提取物莽草酸抗甲型流感病毒的体外研究[J].遵义医学院学报,2013,36(6):503-506.
[6]冷红英,吴昀,胡莹.江苏省脊髓灰质炎实验室细胞系敏感性实验方法建立和监测[J].中国初级卫生保健,2014,28(1):77-78.
[7]莫红缨,柯昌文,郑劲平,等.连花清瘟胶囊体外抗甲型IV的实验研究[J].中药新药与临床药理,2007,18(1):5-9.
[8]于海生,陈文直,王嫣,等.低强度脉冲超声对体外培养的骨髓间充质干细胞增殖效应的研究[J].中国医科大学学报,2011,40(11):971-974.
[9]徐琦,何蕾,罗鹏,等.流行性感冒病毒监测及检测技术的研究进展[J].现代生物医学进展,2014,33(14):6597-6600.
[10]张耕实,祁贤,卢协勤,等.栀子苷对甲型H1N1流感病毒的抑制作用[J].中国药科大学学报,2016,47(2):204-209.