中国小型猪内源性反转录病毒研究进展与对策
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摘要
作者介绍了国内外猪内源性反转录病毒(PERV)研究进展,简述了中国不同品种小型猪PERV存在情况、PERV前病毒全基因克隆与序列分析、细胞水平鉴定方法、感染HEK293细胞研究平台的创建、猪A3F对PERV的抑制作用研究,以及从分子遗传学上揭示不同品系PERV特异性等创新性研究成果,分析了五指山小型猪近交系具有PERV基因拷贝少且没有PERV-C等特异性,以及展望培育中国PERV阴性猪新品系方法等研究,以期为人类异种器官移植和生物医用材料产品安全性研发,解决小型猪PERV疾病传播危险性提供科学对策和新的方向。
The paper is a brief introduction of porcine endogenous retrovirus(PERV)innovative research results in the world and also expounds the passing PERV existence situation on different varieties of miniature pig,analyzes the PERV-virus gene cloning and sequence,appraises method on cell level,create the platform of infection HEK293 cells research,study on pigs A3 Finhibition of PERV,and reveal the innovative research results on the specific molecular genetics in the different strain of PERV,analyzes the advantages of Wuzhishan miniature pig inbred line such as low gene copy of PERV,and there is no passing PERV-C specificity and as well as looking forward to cultivate the methods for new strain of PERV negative pigs.It will provide a scientific counter measure and new perspective to solve the spread of disease risk of miniature pig PERV and product safety for human xenotransplantation and biomedical materials of research and development.
The paper is a brief introduction of porcine endogenous retrovirus(PERV)innovative research results in the world and also expounds the passing PERV existence situation on different varieties of miniature pig,analyzes the PERV-virus gene cloning and sequence,appraises method on cell level,create the platform of infection HEK293 cells research,study on pigs A3 Finhibition of PERV,and reveal the innovative research results on the specific molecular genetics in the different strain of PERV,analyzes the advantages of Wuzhishan miniature pig inbred line such as low gene copy of PERV,and there is no passing PERV-C specificity and as well as looking forward to cultivate the methods for new strain of PERV negative pigs.It will provide a scientific counter measure and new perspective to solve the spread of disease risk of miniature pig PERV and product safety for human xenotransplantation and biomedical materials of research and development.
引文
[1]冯书堂,高倩,刘岚.哺乳动物近交系资源创新百年[J].遗传,2016,38(3):181-195.
[2]陈华.小型猪医学研究模型的建立与应用[M].北京:人民卫生出版社,2015.
[3]Takeuchi Y,Patience C,Magre S,et al.Host range and interference studies of three classes of pig endogenous retrovirus[J].J Virol,1998,72(12):9986-9991.
[4]刘广波.异种移植相关的猪内源性逆转录病毒的调查和实验研究[D].广州:南方医科大学,2012.
[5]冯书堂.中国五指山小型猪[M].北京:中国农业科学出版社,1999.
[6]李幼平.移植免疫生物学[M].北京:北京科学出版社,2000.
[7]Patience C,Takeuchi Y,Weiss R.Infection of human cells by an endogenous retrovirus of pigs[J].Nat Med,1997,3:282-286.
[8]Kuddus R H,Gandhi C R,Rehman K K,et al.Some morphological,growth,and genomic properties of human cells chronically infected with porcine endogenous retrovirus(PERV)[J].Genome,2003,46(5):858-869.
[9]Martin U,Kiessig V,Blusch J H,et a1.Expression of pig endogenous retrovirus by primary porcine endothelial cells and infection of human cells[J].The Lancet,1998,352(29):692-694.
[10]吴敏霞.我国小型猪内源性反转录病毒的检测与五指山毒株全长cDNA克隆的构建[D].南宁:广西大学,2005.
[11]Ramsoondar J,Vaught T,Ball S,et al.Production of transgenic pigs that express porcine endogenous retrovirus small interfering RNAs[J].Xenotransplantation,2009,16(3):164-180.
[12]Watanabe M,Umeyama K,Matsunari H,et al.Knockout of exogenous EGFP gene inporcine somatic cells using zinc-finger nucleases[J].Biochem Biophys Res Commun,2010,402:14-18.
[13]Dieckhoff B,Petersen B,Kues W A,et al.Knockdown of porcineendogenous retrovirus(PERV)expression by PERV-specific shRNA in transgenic pigs[J].Xenotransplantation,2008,15(1):36-45.
[14]Ma Y,Yang Y,Lv M,et al.Real-time quantitative polymerase chain reaction with SYBR greenⅠdetection for estimating copy numbers of porcine endogenous retrovirus from Chinese miniature pigs[J].Transplantation Proceedings,2010,42(5):1949-1952.
[15]何凯,欧江涛,黄礼光,等.内源性逆转录病毒在五指山小型猪体内存在状况的分子微生物学调查[J].中国兽医学报,2008,28(1),68-71.
[16]张念,马玉媛,向思龙,等.五指山小型猪内源性反转录病毒囊膜基因变异特征研究[J].中国预防兽医学报,2013,35(3):248-250.
[17]Lv M M,Xu S,Wu J M,et al.Identification of fulllength proviral DNA of porcine endogenousJingang retrovirus from Chinese Wuzhishan miniature pigs inbred[J].Comp Immunol Microbiol Infect Dis,2010,33(4):323-331.
[18]Dermer J.Recombinant porcine endogenous retroviruses(PERV-A/C):A new risk for xenotransplantation[J].Arch Virol,2008,153(8):1421-1426.
[19]Fang X D,Mou Y L,Huang Z Y,et al.The sequence and analysis of a Chinese pig genome[J].Giga Science,2012,1(1):16.
[20]冯书堂,李奎,牟玉莲,等.五指山小型猪近交系培育与遗传资源创新[J].农业生物技术学报,2012,20(8):849-857.
[2]陈华.小型猪医学研究模型的建立与应用[M].北京:人民卫生出版社,2015.
[3]Takeuchi Y,Patience C,Magre S,et al.Host range and interference studies of three classes of pig endogenous retrovirus[J].J Virol,1998,72(12):9986-9991.
[4]刘广波.异种移植相关的猪内源性逆转录病毒的调查和实验研究[D].广州:南方医科大学,2012.
[5]冯书堂.中国五指山小型猪[M].北京:中国农业科学出版社,1999.
[6]李幼平.移植免疫生物学[M].北京:北京科学出版社,2000.
[7]Patience C,Takeuchi Y,Weiss R.Infection of human cells by an endogenous retrovirus of pigs[J].Nat Med,1997,3:282-286.
[8]Kuddus R H,Gandhi C R,Rehman K K,et al.Some morphological,growth,and genomic properties of human cells chronically infected with porcine endogenous retrovirus(PERV)[J].Genome,2003,46(5):858-869.
[9]Martin U,Kiessig V,Blusch J H,et a1.Expression of pig endogenous retrovirus by primary porcine endothelial cells and infection of human cells[J].The Lancet,1998,352(29):692-694.
[10]吴敏霞.我国小型猪内源性反转录病毒的检测与五指山毒株全长cDNA克隆的构建[D].南宁:广西大学,2005.
[11]Ramsoondar J,Vaught T,Ball S,et al.Production of transgenic pigs that express porcine endogenous retrovirus small interfering RNAs[J].Xenotransplantation,2009,16(3):164-180.
[12]Watanabe M,Umeyama K,Matsunari H,et al.Knockout of exogenous EGFP gene inporcine somatic cells using zinc-finger nucleases[J].Biochem Biophys Res Commun,2010,402:14-18.
[13]Dieckhoff B,Petersen B,Kues W A,et al.Knockdown of porcineendogenous retrovirus(PERV)expression by PERV-specific shRNA in transgenic pigs[J].Xenotransplantation,2008,15(1):36-45.
[14]Ma Y,Yang Y,Lv M,et al.Real-time quantitative polymerase chain reaction with SYBR greenⅠdetection for estimating copy numbers of porcine endogenous retrovirus from Chinese miniature pigs[J].Transplantation Proceedings,2010,42(5):1949-1952.
[15]何凯,欧江涛,黄礼光,等.内源性逆转录病毒在五指山小型猪体内存在状况的分子微生物学调查[J].中国兽医学报,2008,28(1),68-71.
[16]张念,马玉媛,向思龙,等.五指山小型猪内源性反转录病毒囊膜基因变异特征研究[J].中国预防兽医学报,2013,35(3):248-250.
[17]Lv M M,Xu S,Wu J M,et al.Identification of fulllength proviral DNA of porcine endogenousJingang retrovirus from Chinese Wuzhishan miniature pigs inbred[J].Comp Immunol Microbiol Infect Dis,2010,33(4):323-331.
[18]Dermer J.Recombinant porcine endogenous retroviruses(PERV-A/C):A new risk for xenotransplantation[J].Arch Virol,2008,153(8):1421-1426.
[19]Fang X D,Mou Y L,Huang Z Y,et al.The sequence and analysis of a Chinese pig genome[J].Giga Science,2012,1(1):16.
[20]冯书堂,李奎,牟玉莲,等.五指山小型猪近交系培育与遗传资源创新[J].农业生物技术学报,2012,20(8):849-857.