两种多糖对齐口裂腹鱼免疫性能的影响
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摘要
探讨OKGM、OKGMS两种多糖对齐口裂腹鱼免疫性能的影响,将齐口裂腹鱼随机分为7组,两种多糖添加剂量分别为质量分数0.4%、0.8%、1.6%,饲喂60 d,测定齐口裂腹鱼血清免疫及抗氧化指标,并采用RT-PCR技术检测组织MyD88、IRF7基因相对表达量。结果表明,OKGMS_(1.6)组能显著提高齐口裂腹鱼血清IgM活性、ACH50含量;OKGMS_(0.4)、OKGMS_(0.8)、OKGM_(0.4)、OKGM_(0.8)组均能显著降低血清MDA的含量;OKGMS与OKGM均能提高血清SOD活性;OKGMS能上调齐口裂腹鱼肠道MyD88基因mRNA相对表达量及肝脏、脾脏、中肾IRF7基因mRNA相对表达量;OKGM能上调肠道、头肾、中肾MyD88基因mRNA相对表达量及肠道、肝脏、脾脏、中肾IRF7基因m RNA相对表达量。结果表明:适量添加OKGM、OKGMS能提高齐口裂腹鱼抗氧化活性及免疫性能。
The experiment was conducted to investigate the effects of oxidized konjac glucomannan and oxidized konjac glucomannan sulfates on immune performance of Schizothorax prenanti.The Schizothorax prenanti were randomly divided into 7 groups and each group included 3 replicates of20 fish.The experiment lasted for 60 days with the treatment groups were contained 0.4%、0.8%、1.6%of OKGM and OKGMS.And body composition and serum biochemical indices were measured and My D88、IRF7 mRNA level in tissue were determined by RT-PCR.Results:Content of Ig M、ACH50 of OKGMS1.6 were significantly more than the control group;The content of MDA was significantly lower of group OKGMS_(0.4)、OKGMS_(0.8)、OKGM_(0.4)、OKGM_(0.8)than the control group;OKGMS、OKGM both increased the activity of SOD;OKGMS could up-regulate My D88 mRNA level in gut and IRF7 mRNA level in liver、spleen and mesonephros;OKGM could increase My D88mRNA level in gut、headkidney and mesonephros and IRF7 mRNA level in gut、liver、spleen and mesonephros.Conclusions:Add the appropriate amount of OKGM and OKGMS could increase the antioxidant activity and immune functions of Schizothorax prenanti.
The experiment was conducted to investigate the effects of oxidized konjac glucomannan and oxidized konjac glucomannan sulfates on immune performance of Schizothorax prenanti.The Schizothorax prenanti were randomly divided into 7 groups and each group included 3 replicates of20 fish.The experiment lasted for 60 days with the treatment groups were contained 0.4%、0.8%、1.6%of OKGM and OKGMS.And body composition and serum biochemical indices were measured and My D88、IRF7 mRNA level in tissue were determined by RT-PCR.Results:Content of Ig M、ACH50 of OKGMS1.6 were significantly more than the control group;The content of MDA was significantly lower of group OKGMS_(0.4)、OKGMS_(0.8)、OKGM_(0.4)、OKGM_(0.8)than the control group;OKGMS、OKGM both increased the activity of SOD;OKGMS could up-regulate My D88 mRNA level in gut and IRF7 mRNA level in liver、spleen and mesonephros;OKGM could increase My D88mRNA level in gut、headkidney and mesonephros and IRF7 mRNA level in gut、liver、spleen and mesonephros.Conclusions:Add the appropriate amount of OKGM and OKGMS could increase the antioxidant activity and immune functions of Schizothorax prenanti.
引文
[1]LIN Huimin,PANG Jie,FAN Linlin,et al.Advances in immunological activities of Konjac glucomannan[J].Chinese Pharmacological Bulletin,2010(11):1404-1406.(in Chinese)
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[3]SHEN Jie,LIU Yujun,ZHANG Jue.Sulfated modification of extracellular polysaccharide from submerged fermentation of ganoderma lucidum[J].Journal of Food Science and Biotechnology,2015(6):666-671.(in Chinese)
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[8]ZHANG L,WU Y,WANG L,et al.Effects of oxidized konjac glucomannan(OKGM)on growth and immune function of Schizothorax prenanti[J].Fish&Shellfish Immunology,2013,35(4):1105-1110.
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[11]ZHENG Q,WU Y,XU H.Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance,body composition,intestinal morphology and intestinal microflora[J].Fish Physiology and Biochemistry,2015,41(3):733-743.
[12]VAN MUISWINKEL W B,NAKAO M.A short history of research on immunity to infectious diseases in fish[J].Developmental&Comparative Immunology,2014,43(2):130-150.
[13]CEREZUELA R,GUARDIOLA F A,GONZALEZ P,et al.Effects of dietary Bacillus subtilis,Tetraselmis chuii,and Phaeodactylum tricornutum,singularly or in combination,on the immune response and disease resistance of sea bream(Sparus aurata L.)[J].Fish&Shellfish Immunology,2012,33(2):342-349.
[14]ARAMLI M S,KAMANGAR B,NAZARI R M.Effects of dietaryβ-glucan on the growth and innate immune response of juvenile Persian sturgeon,Acipenser persicus[J].Fish&Shellfish Immunology,2015,47(1):606-610.
[15]ZHENG Q,WU Y,XU H,et al.The effects of dietary oxidized konjac glucomannan and its acidolysis products on the immune response,expression of immune related genes and disease resistance of Schizothorax prenanti[J].Fish&Shellfish Immunology,2015,45(2):551-559.
[16]BAI Dongqing,WU Xuan,GUO Yongjun,et al.Effect of astragalus polysaccharides on antioxidant and nonspecific immune indices of yellow catfish(pelteobagrus fulvidraco)over long-term feeding[J].Chinese Journal of Animal Nutrition,2011(9):1622-1630.(in Chinese)
[17]XU F,ZHANG Y,LI J,et al.Expression and function analysis of two naturally truncated My D88 variants in the Pacific oyster Crassostrea gigas[J].Fish&Shellfish Immunology,2015,45(2):510-516.
[18]SUN H,JIANG Z,MAO M,et al.Cloning and expression analysis of interferon regulatory factor 7 in the Pacific cod,Gadus macrocephalus[J].Fish&Shellfish Immunology,2016,49:7-15.
[19]HU G,XIA J,LOU H,et al.Cloning and expression analysis of interferon regulatory factor 7(IRF-7)in turbot,Scophthalmus maximus[J].Developmental&Comparative Immunology,2011,35(4):416-420.
[20]FENG Z,WANG Z,YANG M,et al.Polysaccharopeptide exerts immunoregulatory effects via My D88-dependent signaling pathway[J].International Journal of Biological Macromolecules,2016,82:201-207.
[2]HUANG Mingfa,ZHANG Shenglin.Healthy functions of Konjac dietary fiber[J].Food and Nutrition in China,2010(5):75-77.(in Chinese)
[3]SHEN Jie,LIU Yujun,ZHANG Jue.Sulfated modification of extracellular polysaccharide from submerged fermentation of ganoderma lucidum[J].Journal of Food Science and Biotechnology,2015(6):666-671.(in Chinese)
[4]MAO Gennian,QU Jianbo,LI Xin,et al.Research progress in preparation technique of mannose-oligosaccharides and its derivatives[J].Science and Technology of Food Industry,2011(2):366-368.(in Chinese)
[5]LIU Apeng,JIANG Yongming,ZHANG Li,et al.Effects of oligo-konjac glucomannansulfate on immunological activities of penaeus vannamei[J].Modern Agricultural Science and Technology,2012(13):268-269.(in Chinese)
[6]XIA Xiaojie,WU Yinglong,FENG Jiao,et al.Effect of two kinds of polysaccharide on growth performance,body composition and serum biochemical indices of schizothorax prenanti tchang[J].Food Science,2015(17):240-246.(in Chinese)
[7]ZOU Zhiying,WANG Qian,YANG Hong,et al.Cloning and expression of a myeloid differentiation factor 88 gene in tilapia Oreochromis aureus[J].Chinese Journal of Zoology,2013(6):894-904.(in Chinese)
[8]ZHANG L,WU Y,WANG L,et al.Effects of oxidized konjac glucomannan(OKGM)on growth and immune function of Schizothorax prenanti[J].Fish&Shellfish Immunology,2013,35(4):1105-1110.
[9]WANG J,ZHANG L,YU Y,et al.Enhancement of antitumor activities in sulfated and carboxymethylated polysaccharides of ganoderma lucidum[J].Journal of Agricultural and Food Chemistry,2009,57(22):10565-10572.
[10]ZOU C,DU Y,LI Y,et al.Preparation of lacquer polysaccharide sulfates and their antioxidant activity in vitro[J].Carbohydrate Polymers,2008,73(2):322-331.
[11]ZHENG Q,WU Y,XU H.Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance,body composition,intestinal morphology and intestinal microflora[J].Fish Physiology and Biochemistry,2015,41(3):733-743.
[12]VAN MUISWINKEL W B,NAKAO M.A short history of research on immunity to infectious diseases in fish[J].Developmental&Comparative Immunology,2014,43(2):130-150.
[13]CEREZUELA R,GUARDIOLA F A,GONZALEZ P,et al.Effects of dietary Bacillus subtilis,Tetraselmis chuii,and Phaeodactylum tricornutum,singularly or in combination,on the immune response and disease resistance of sea bream(Sparus aurata L.)[J].Fish&Shellfish Immunology,2012,33(2):342-349.
[14]ARAMLI M S,KAMANGAR B,NAZARI R M.Effects of dietaryβ-glucan on the growth and innate immune response of juvenile Persian sturgeon,Acipenser persicus[J].Fish&Shellfish Immunology,2015,47(1):606-610.
[15]ZHENG Q,WU Y,XU H,et al.The effects of dietary oxidized konjac glucomannan and its acidolysis products on the immune response,expression of immune related genes and disease resistance of Schizothorax prenanti[J].Fish&Shellfish Immunology,2015,45(2):551-559.
[16]BAI Dongqing,WU Xuan,GUO Yongjun,et al.Effect of astragalus polysaccharides on antioxidant and nonspecific immune indices of yellow catfish(pelteobagrus fulvidraco)over long-term feeding[J].Chinese Journal of Animal Nutrition,2011(9):1622-1630.(in Chinese)
[17]XU F,ZHANG Y,LI J,et al.Expression and function analysis of two naturally truncated My D88 variants in the Pacific oyster Crassostrea gigas[J].Fish&Shellfish Immunology,2015,45(2):510-516.
[18]SUN H,JIANG Z,MAO M,et al.Cloning and expression analysis of interferon regulatory factor 7 in the Pacific cod,Gadus macrocephalus[J].Fish&Shellfish Immunology,2016,49:7-15.
[19]HU G,XIA J,LOU H,et al.Cloning and expression analysis of interferon regulatory factor 7(IRF-7)in turbot,Scophthalmus maximus[J].Developmental&Comparative Immunology,2011,35(4):416-420.
[20]FENG Z,WANG Z,YANG M,et al.Polysaccharopeptide exerts immunoregulatory effects via My D88-dependent signaling pathway[J].International Journal of Biological Macromolecules,2016,82:201-207.