基于电力聚焦的光纤光镊细胞分选
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摘要
提出了基于电力聚焦的单模光纤耦合激光分选细胞的方法。基于电力聚焦模型和光镊原理,推导出中心聚焦流宽度公式和光纤光镊对细胞的散射力公式。在Comsol Multiphysics 5.3中建立层流、电流、粒子追踪和电磁波四个物理场,进行了直径10μm和20μm粒子的单细胞流和分选仿真。在此基础上,搭建了光纤光镊分选细胞实验平台,配置了酵母菌细胞和聚苯乙烯微球的混合溶液,进行了实验。仿真和实验结果均表明,在入口电压比1∶1.2的情况下,在主通道中能形成间距大致均匀相等的单细胞流。并在波长为980 nm、功率为300 mW激光条件下,聚焦激光可以通过单模光纤驱动不同直径的细胞沿光轴方向移动,且移动距离不相等,从而迫使细胞进入不同的通道进行分选。
A method of cell sorting by single mode fiber coupled laser based on the electric focusing was proposed.Based on the electric focusing model and optical tweezers principle,the formulas of the central focusing flow width and scattering force of optical tweezers on cells were derived.In Comsol Multiphysics 5.3,the four physical fields of the laminar flow,electric current,particle tracking and electromagnetic wave were set up.The simulations of single cell flow and sorting of particles with the diameters of 10μm and20μm were carried out,respectively.On this basis,an experimental platform for cell sorting by optical tweezers was built,and the mixed solution of yeast cells and polystyrene microspheres was prepared to carry out the experiment.The simulation and experimental results show that the single cell flow with approximately uniform spacing can be formed in the main channel at the input voltage ratio of 1∶1.2.Under the condition of 980 nm wavelength and300 mW laser power,the focused laser can drive the cells with different diameters to move along the optical axis through single-mode optical fibers,and the moving distances are not equal.Thus,the cells are forced to enter different channels for sorting.
A method of cell sorting by single mode fiber coupled laser based on the electric focusing was proposed.Based on the electric focusing model and optical tweezers principle,the formulas of the central focusing flow width and scattering force of optical tweezers on cells were derived.In Comsol Multiphysics 5.3,the four physical fields of the laminar flow,electric current,particle tracking and electromagnetic wave were set up.The simulations of single cell flow and sorting of particles with the diameters of 10μm and20μm were carried out,respectively.On this basis,an experimental platform for cell sorting by optical tweezers was built,and the mixed solution of yeast cells and polystyrene microspheres was prepared to carry out the experiment.The simulation and experimental results show that the single cell flow with approximately uniform spacing can be formed in the main channel at the input voltage ratio of 1∶1.2.Under the condition of 980 nm wavelength and300 mW laser power,the focused laser can drive the cells with different diameters to move along the optical axis through single-mode optical fibers,and the moving distances are not equal.Thus,the cells are forced to enter different channels for sorting.
引文
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[2]林炳承.微纳流控芯片实验室[M].北京:科学出版社,2015:364-366.
[3]李银妹,姚焜.光镊技术[M].北京:科学出版社,2015:223.
[4]CHENG J M,YANG L,ZHOU W,et al.Analysis on influence parameters of cell sorting using optical pressure difference technology[J].Optics and Precision Engineering,2017,25(8):2029-2037.
[5]LANDENBERGER B,HOFEMANN H,WADLE S,et al.Microfluidic sorting of arbitrary cells with dynamic optical tweezers[J].Lab on a Chip,2012,12(17):3177-3183.
[6]WANG X L,CHEN S X,KONG C W,et al.Enhanced cell sorting and manipulation with combined optical tweezer and microfluidic chip technologies[J].Lab on a Chip,2011,11(21):3656-3662.
[7]SUN T,KOVAC J,VOLDMAN J.Image-based single-cell sorting via dual-photopoly merize microwell arrays[J].Analytical Chemistry,2014,86(2):977-981.
[8]LEE G B,HUNG C I,KE B J,et al.Hydrodynamic focusing for a micromachined flow cytometer[J].Journal of Fluids Engineering,2001,123(3):672-679.
[9]JACOBSON S C,RAMSEY J M.Electrokinetic focus in micro-fabricated channel structures[J].Analytical Chemistry,1997,69(16):3212-3217.
[10]张英杰,张思祥,李新冉.用于光镊分选基于电力聚焦的微通道单细胞流[J].微纳电子技术,2018,55(8):583-587.
[11]YANG R J,CHANG C C,HUANG S B,et al.A new focusing model and switching approach for electrokinetic flow inside microchannels[J].Journal of Micromechanics and Microengineering,2005,15(11):2141-2148.
[12]张英杰,张思祥,竭霞.基于T矩阵模型的光纤芯片的制备及其细胞驱动[J].微纳电子技术,2019,56(1):45-50.
[13]CONSTABLE A,KIM J,MERVIS J,et al.Demonstration of fiber-optical light-force trap[J].Optics Letters,1993,18(21):1867-1869.